Potential cross-contamination of similar Giardia duodenalis assemblage in children and pet dogs in southern Brazil, as determined by PCR-RFLP

Detalhes bibliográficos
Autor(a) principal: Quadros, Rosiléia Marinho de
Data de Publicação: 2016
Outros Autores: Weiss, Paulo Henrique Exterckotter, Marques, Sandra Marcia Tietz, Miletti, Luiz Claudio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/152692
Resumo: Giardia duodenalis is an enteric parasite that has distinct genetic groups. Human infections are mainly caused by assemblages A and B, although sporadic infections by assemblages C and D have also been reported. Animals can be infected by a wide range of assemblages (A to H). The aim of this study is to identify the assemblages and sub-assemblages of G. duodenalis with zoonotic features in fecal samples of school-aged children, and in dogs that coexist in the same households in Lages, Santa Catarina, Brazil. Fecal samples of 91 children and 108 dogs were obtained and G. duodenalis cysts were detected in samples from 11 (12.08%) children and 10 (9.25%) dogs. DNA extracted from the 21 positive samples was analyzed by PCR-RFLP, using the gdh gene. Results showed the presence of sub-assemblages AI (2/11), AII (4/11), BIII (2/11), and BIV(3/11) among children and AI (5/10) and BIV(3/10) in dogs, with zoonotic characteristics, and the carnivore specific assemblage C (2/10). G. duodenalis was found to infect both children and dogs living in the same household, with the same sub-assemblage (BIV) indicating that pet dogs are a potential risk of transmission of G. duodenalis to humans.
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spelling Quadros, Rosiléia Marinho deWeiss, Paulo Henrique ExterckotterMarques, Sandra Marcia TietzMiletti, Luiz Claudio2017-02-17T02:33:05Z20160036-4665http://hdl.handle.net/10183/152692001009131Giardia duodenalis is an enteric parasite that has distinct genetic groups. Human infections are mainly caused by assemblages A and B, although sporadic infections by assemblages C and D have also been reported. Animals can be infected by a wide range of assemblages (A to H). The aim of this study is to identify the assemblages and sub-assemblages of G. duodenalis with zoonotic features in fecal samples of school-aged children, and in dogs that coexist in the same households in Lages, Santa Catarina, Brazil. Fecal samples of 91 children and 108 dogs were obtained and G. duodenalis cysts were detected in samples from 11 (12.08%) children and 10 (9.25%) dogs. DNA extracted from the 21 positive samples was analyzed by PCR-RFLP, using the gdh gene. Results showed the presence of sub-assemblages AI (2/11), AII (4/11), BIII (2/11), and BIV(3/11) among children and AI (5/10) and BIV(3/10) in dogs, with zoonotic characteristics, and the carnivore specific assemblage C (2/10). G. duodenalis was found to infect both children and dogs living in the same household, with the same sub-assemblage (BIV) indicating that pet dogs are a potential risk of transmission of G. duodenalis to humans.application/pdfengRevista do Instituto de Medicina Tropical de São Paulo = Journal of the São Paulo Institute of Tropical Medicine. São Paulo. Vol. 58 (2016), [7] p.Giardia duodenalisZoonoses : Animais domesticosParasitologia veterinaria : Animais domesticosGiardia duodenalisPet dogsZoonotic genotypesPCR-RFLPPotential cross-contamination of similar Giardia duodenalis assemblage in children and pet dogs in southern Brazil, as determined by PCR-RFLPinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001009131.pdf001009131.pdfTexto completo (inglês)application/pdf309638http://www.lume.ufrgs.br/bitstream/10183/152692/1/001009131.pdf28bd329be0b4dae5620ed15b883eb66dMD51TEXT001009131.pdf.txt001009131.pdf.txtExtracted Texttext/plain32208http://www.lume.ufrgs.br/bitstream/10183/152692/2/001009131.pdf.txta6adfd31ff55c82ba9d038c0cd84c8bbMD5210183/1526922017-02-18 02:43:09.499542oai:www.lume.ufrgs.br:10183/152692Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2017-02-18T04:43:09Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Potential cross-contamination of similar Giardia duodenalis assemblage in children and pet dogs in southern Brazil, as determined by PCR-RFLP
title Potential cross-contamination of similar Giardia duodenalis assemblage in children and pet dogs in southern Brazil, as determined by PCR-RFLP
spellingShingle Potential cross-contamination of similar Giardia duodenalis assemblage in children and pet dogs in southern Brazil, as determined by PCR-RFLP
Quadros, Rosiléia Marinho de
Giardia duodenalis
Zoonoses : Animais domesticos
Parasitologia veterinaria : Animais domesticos
Giardia duodenalis
Pet dogs
Zoonotic genotypes
PCR-RFLP
title_short Potential cross-contamination of similar Giardia duodenalis assemblage in children and pet dogs in southern Brazil, as determined by PCR-RFLP
title_full Potential cross-contamination of similar Giardia duodenalis assemblage in children and pet dogs in southern Brazil, as determined by PCR-RFLP
title_fullStr Potential cross-contamination of similar Giardia duodenalis assemblage in children and pet dogs in southern Brazil, as determined by PCR-RFLP
title_full_unstemmed Potential cross-contamination of similar Giardia duodenalis assemblage in children and pet dogs in southern Brazil, as determined by PCR-RFLP
title_sort Potential cross-contamination of similar Giardia duodenalis assemblage in children and pet dogs in southern Brazil, as determined by PCR-RFLP
author Quadros, Rosiléia Marinho de
author_facet Quadros, Rosiléia Marinho de
Weiss, Paulo Henrique Exterckotter
Marques, Sandra Marcia Tietz
Miletti, Luiz Claudio
author_role author
author2 Weiss, Paulo Henrique Exterckotter
Marques, Sandra Marcia Tietz
Miletti, Luiz Claudio
author2_role author
author
author
dc.contributor.author.fl_str_mv Quadros, Rosiléia Marinho de
Weiss, Paulo Henrique Exterckotter
Marques, Sandra Marcia Tietz
Miletti, Luiz Claudio
dc.subject.por.fl_str_mv Giardia duodenalis
Zoonoses : Animais domesticos
Parasitologia veterinaria : Animais domesticos
topic Giardia duodenalis
Zoonoses : Animais domesticos
Parasitologia veterinaria : Animais domesticos
Giardia duodenalis
Pet dogs
Zoonotic genotypes
PCR-RFLP
dc.subject.eng.fl_str_mv Giardia duodenalis
Pet dogs
Zoonotic genotypes
PCR-RFLP
description Giardia duodenalis is an enteric parasite that has distinct genetic groups. Human infections are mainly caused by assemblages A and B, although sporadic infections by assemblages C and D have also been reported. Animals can be infected by a wide range of assemblages (A to H). The aim of this study is to identify the assemblages and sub-assemblages of G. duodenalis with zoonotic features in fecal samples of school-aged children, and in dogs that coexist in the same households in Lages, Santa Catarina, Brazil. Fecal samples of 91 children and 108 dogs were obtained and G. duodenalis cysts were detected in samples from 11 (12.08%) children and 10 (9.25%) dogs. DNA extracted from the 21 positive samples was analyzed by PCR-RFLP, using the gdh gene. Results showed the presence of sub-assemblages AI (2/11), AII (4/11), BIII (2/11), and BIV(3/11) among children and AI (5/10) and BIV(3/10) in dogs, with zoonotic characteristics, and the carnivore specific assemblage C (2/10). G. duodenalis was found to infect both children and dogs living in the same household, with the same sub-assemblage (BIV) indicating that pet dogs are a potential risk of transmission of G. duodenalis to humans.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-02-17T02:33:05Z
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dc.identifier.nrb.pt_BR.fl_str_mv 001009131
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dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo = Journal of the São Paulo Institute of Tropical Medicine. São Paulo. Vol. 58 (2016), [7] p.
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