Molecular evolution and structural analyses of proteins involved in metabolic pathways of volatile organic compounds in Petunia hybrida (Solanaceae)
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/266630 |
Resumo: | The association between plants and their pollinators is essential for increasing the diversity in angiosperms. Morphological and physiological traits, mainly floral scent, can influence the pollination dynamics and select pollinators for each plant species. In this work, we studied two proteins involved in producing volatile organic compounds in plants, conyferyl alcohol acyltransferase (CFAT) and benzoyl-CoA:benzyl alcohol/phenyl ethanol benzoyl transferase (BPBT) genes. We aimed to understand these proteins with respect to evolutionary and structural aspects and functions in Solanaceae using phylogenetic methods and comparative molecular modeling. We used Bayesian inference to describe the proteins’ evolutionary history using Petunia x hybrida as a query to search for homologs in the Solanaceae family. Theoretical 3D models were obtained for both proteins using Panicum virgatum as a template. The phylogenetic tree included several different enzymes with diverse biological roles in Solanaceae, displaying the transferase domain. We identified only one sequence of CFAT in the databases, which belongs to Petunia x hybrida, and found several BPBT sequences from the genera Nicotiana, Solanum, and Capsicum. The 3D structures of CFAT and BPBT have two different domains, and we have identified the amino acid residues essential for the enzymatic activity and interaction with substrates. |
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Beltrame, Lucas CafferatiThompson, Claudia ElizabethFreitas, Loreta Brandão de2023-11-04T03:35:26Z20231415-4757http://hdl.handle.net/10183/266630001170420The association between plants and their pollinators is essential for increasing the diversity in angiosperms. Morphological and physiological traits, mainly floral scent, can influence the pollination dynamics and select pollinators for each plant species. In this work, we studied two proteins involved in producing volatile organic compounds in plants, conyferyl alcohol acyltransferase (CFAT) and benzoyl-CoA:benzyl alcohol/phenyl ethanol benzoyl transferase (BPBT) genes. We aimed to understand these proteins with respect to evolutionary and structural aspects and functions in Solanaceae using phylogenetic methods and comparative molecular modeling. We used Bayesian inference to describe the proteins’ evolutionary history using Petunia x hybrida as a query to search for homologs in the Solanaceae family. Theoretical 3D models were obtained for both proteins using Panicum virgatum as a template. The phylogenetic tree included several different enzymes with diverse biological roles in Solanaceae, displaying the transferase domain. We identified only one sequence of CFAT in the databases, which belongs to Petunia x hybrida, and found several BPBT sequences from the genera Nicotiana, Solanum, and Capsicum. The 3D structures of CFAT and BPBT have two different domains, and we have identified the amino acid residues essential for the enzymatic activity and interaction with substrates.application/pdfengGenetics and molecular biology. Ribeirão Preto. Vol. 46, no. 1 suppl. 1 (2023), e20220114, 10 p.Compostos orgânicos voláteisSolanaceaeEvolução molecularProtein structureMolecular evolution and structural analyses of proteins involved in metabolic pathways of volatile organic compounds in Petunia hybrida (Solanaceae)info:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001170420.pdf.txt001170420.pdf.txtExtracted Texttext/plain43874http://www.lume.ufrgs.br/bitstream/10183/266630/2/001170420.pdf.txt7b7f71b211c9e4709a9a750b676cb50eMD52ORIGINAL001170420.pdfTexto completo (inglês)application/pdf2482827http://www.lume.ufrgs.br/bitstream/10183/266630/1/001170420.pdf3aea34de625e839790a5cc3664faf9e3MD5110183/2666302024-05-23 06:43:26.545221oai:www.lume.ufrgs.br:10183/266630Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-05-23T09:43:26Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Molecular evolution and structural analyses of proteins involved in metabolic pathways of volatile organic compounds in Petunia hybrida (Solanaceae) |
title |
Molecular evolution and structural analyses of proteins involved in metabolic pathways of volatile organic compounds in Petunia hybrida (Solanaceae) |
spellingShingle |
Molecular evolution and structural analyses of proteins involved in metabolic pathways of volatile organic compounds in Petunia hybrida (Solanaceae) Beltrame, Lucas Cafferati Compostos orgânicos voláteis Solanaceae Evolução molecular Protein structure |
title_short |
Molecular evolution and structural analyses of proteins involved in metabolic pathways of volatile organic compounds in Petunia hybrida (Solanaceae) |
title_full |
Molecular evolution and structural analyses of proteins involved in metabolic pathways of volatile organic compounds in Petunia hybrida (Solanaceae) |
title_fullStr |
Molecular evolution and structural analyses of proteins involved in metabolic pathways of volatile organic compounds in Petunia hybrida (Solanaceae) |
title_full_unstemmed |
Molecular evolution and structural analyses of proteins involved in metabolic pathways of volatile organic compounds in Petunia hybrida (Solanaceae) |
title_sort |
Molecular evolution and structural analyses of proteins involved in metabolic pathways of volatile organic compounds in Petunia hybrida (Solanaceae) |
author |
Beltrame, Lucas Cafferati |
author_facet |
Beltrame, Lucas Cafferati Thompson, Claudia Elizabeth Freitas, Loreta Brandão de |
author_role |
author |
author2 |
Thompson, Claudia Elizabeth Freitas, Loreta Brandão de |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Beltrame, Lucas Cafferati Thompson, Claudia Elizabeth Freitas, Loreta Brandão de |
dc.subject.por.fl_str_mv |
Compostos orgânicos voláteis Solanaceae Evolução molecular |
topic |
Compostos orgânicos voláteis Solanaceae Evolução molecular Protein structure |
dc.subject.eng.fl_str_mv |
Protein structure |
description |
The association between plants and their pollinators is essential for increasing the diversity in angiosperms. Morphological and physiological traits, mainly floral scent, can influence the pollination dynamics and select pollinators for each plant species. In this work, we studied two proteins involved in producing volatile organic compounds in plants, conyferyl alcohol acyltransferase (CFAT) and benzoyl-CoA:benzyl alcohol/phenyl ethanol benzoyl transferase (BPBT) genes. We aimed to understand these proteins with respect to evolutionary and structural aspects and functions in Solanaceae using phylogenetic methods and comparative molecular modeling. We used Bayesian inference to describe the proteins’ evolutionary history using Petunia x hybrida as a query to search for homologs in the Solanaceae family. Theoretical 3D models were obtained for both proteins using Panicum virgatum as a template. The phylogenetic tree included several different enzymes with diverse biological roles in Solanaceae, displaying the transferase domain. We identified only one sequence of CFAT in the databases, which belongs to Petunia x hybrida, and found several BPBT sequences from the genera Nicotiana, Solanum, and Capsicum. The 3D structures of CFAT and BPBT have two different domains, and we have identified the amino acid residues essential for the enzymatic activity and interaction with substrates. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-11-04T03:35:26Z |
dc.date.issued.fl_str_mv |
2023 |
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info:eu-repo/semantics/article info:eu-repo/semantics/other |
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article |
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http://hdl.handle.net/10183/266630 |
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1415-4757 |
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001170420 |
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http://hdl.handle.net/10183/266630 |
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eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Genetics and molecular biology. Ribeirão Preto. Vol. 46, no. 1 suppl. 1 (2023), e20220114, 10 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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