Somatic embryogenesis as an alternative for in vitro multiplication of Butia odorata from mature zygotic embryos
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/253150 |
Resumo: | Butia odorata is a palm native to southern Brazil and Uruguay, not domesticated, much appreciated for its fruits and economic potential. However, the extractivism and the diffi culty of propagation have led to the decline of natural populations. The objective of this work was to prove the possibility of induction of somatic embryogenesis in B. odorata. Mature zygotic embryos were induced in two media, MS and Y3, combined with auxin 2,4-D and picloram in fi ve concentrations (2,4-D: 0, 361.99, 452.49, 542.99 and 633.48 μM/L, picloram: 0, 50, 150, 300 and 450 μM/L). The results promising during induction with the formation of embryogenic calli and somatic embryos, however the regeneration of them was not effi cient, this may be due to the occurrence of somatic embryos fused during its development. The roots were formed, but the aerial part remained molten, not completing its development. Auxin picloram and Y3 medium provided the most adequate conditions for calogenesis, formation of embryogenic callus and somatic embryos, with concentrations of 150, 300 and 450 μM/L. This is the fi rst description of somatic embryogenesis in B. odorata that will serve as the basis for future research and adjustments of the methodology proposed here. |
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Campos, Samanta Siqueira deScherwinski-Pereira, Jonny EversonBernd, Regina BeatrizFior, Claudimar SidneiSchwarz, Sergio Francisco2022-12-24T05:05:05Z20200001-3765http://hdl.handle.net/10183/253150001149888Butia odorata is a palm native to southern Brazil and Uruguay, not domesticated, much appreciated for its fruits and economic potential. However, the extractivism and the diffi culty of propagation have led to the decline of natural populations. The objective of this work was to prove the possibility of induction of somatic embryogenesis in B. odorata. Mature zygotic embryos were induced in two media, MS and Y3, combined with auxin 2,4-D and picloram in fi ve concentrations (2,4-D: 0, 361.99, 452.49, 542.99 and 633.48 μM/L, picloram: 0, 50, 150, 300 and 450 μM/L). The results promising during induction with the formation of embryogenic calli and somatic embryos, however the regeneration of them was not effi cient, this may be due to the occurrence of somatic embryos fused during its development. The roots were formed, but the aerial part remained molten, not completing its development. Auxin picloram and Y3 medium provided the most adequate conditions for calogenesis, formation of embryogenic callus and somatic embryos, with concentrations of 150, 300 and 450 μM/L. This is the fi rst description of somatic embryogenesis in B. odorata that will serve as the basis for future research and adjustments of the methodology proposed here.application/pdfengAnais da Academia Brasileira de Ciências, Rio de Janeiro, RJ,. Vol. 92, Suppl. 1 (2020), e20181215, 13 p.ButiáEmbriogeneseInducaoArecaceaePicloramPindo palmSomatic embryoSomatic embryogenesis as an alternative for in vitro multiplication of Butia odorata from mature zygotic embryosinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001149888.pdf.txt001149888.pdf.txtExtracted Texttext/plain42466http://www.lume.ufrgs.br/bitstream/10183/253150/2/001149888.pdf.txt765af40545560f3300da40977ed1e21aMD52ORIGINAL001149888.pdfTexto completo (inglês)application/pdf1692630http://www.lume.ufrgs.br/bitstream/10183/253150/1/001149888.pdfd9e1c7957a556a1ec3d6c8dbdb93e5ffMD5110183/2531502022-12-25 05:56:05.237659oai:www.lume.ufrgs.br:10183/253150Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2022-12-25T07:56:05Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Somatic embryogenesis as an alternative for in vitro multiplication of Butia odorata from mature zygotic embryos |
title |
Somatic embryogenesis as an alternative for in vitro multiplication of Butia odorata from mature zygotic embryos |
spellingShingle |
Somatic embryogenesis as an alternative for in vitro multiplication of Butia odorata from mature zygotic embryos Campos, Samanta Siqueira de Butiá Embriogenese Inducao Arecaceae Picloram Pindo palm Somatic embryo |
title_short |
Somatic embryogenesis as an alternative for in vitro multiplication of Butia odorata from mature zygotic embryos |
title_full |
Somatic embryogenesis as an alternative for in vitro multiplication of Butia odorata from mature zygotic embryos |
title_fullStr |
Somatic embryogenesis as an alternative for in vitro multiplication of Butia odorata from mature zygotic embryos |
title_full_unstemmed |
Somatic embryogenesis as an alternative for in vitro multiplication of Butia odorata from mature zygotic embryos |
title_sort |
Somatic embryogenesis as an alternative for in vitro multiplication of Butia odorata from mature zygotic embryos |
author |
Campos, Samanta Siqueira de |
author_facet |
Campos, Samanta Siqueira de Scherwinski-Pereira, Jonny Everson Bernd, Regina Beatriz Fior, Claudimar Sidnei Schwarz, Sergio Francisco |
author_role |
author |
author2 |
Scherwinski-Pereira, Jonny Everson Bernd, Regina Beatriz Fior, Claudimar Sidnei Schwarz, Sergio Francisco |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Campos, Samanta Siqueira de Scherwinski-Pereira, Jonny Everson Bernd, Regina Beatriz Fior, Claudimar Sidnei Schwarz, Sergio Francisco |
dc.subject.por.fl_str_mv |
Butiá Embriogenese Inducao |
topic |
Butiá Embriogenese Inducao Arecaceae Picloram Pindo palm Somatic embryo |
dc.subject.eng.fl_str_mv |
Arecaceae Picloram Pindo palm Somatic embryo |
description |
Butia odorata is a palm native to southern Brazil and Uruguay, not domesticated, much appreciated for its fruits and economic potential. However, the extractivism and the diffi culty of propagation have led to the decline of natural populations. The objective of this work was to prove the possibility of induction of somatic embryogenesis in B. odorata. Mature zygotic embryos were induced in two media, MS and Y3, combined with auxin 2,4-D and picloram in fi ve concentrations (2,4-D: 0, 361.99, 452.49, 542.99 and 633.48 μM/L, picloram: 0, 50, 150, 300 and 450 μM/L). The results promising during induction with the formation of embryogenic calli and somatic embryos, however the regeneration of them was not effi cient, this may be due to the occurrence of somatic embryos fused during its development. The roots were formed, but the aerial part remained molten, not completing its development. Auxin picloram and Y3 medium provided the most adequate conditions for calogenesis, formation of embryogenic callus and somatic embryos, with concentrations of 150, 300 and 450 μM/L. This is the fi rst description of somatic embryogenesis in B. odorata that will serve as the basis for future research and adjustments of the methodology proposed here. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020 |
dc.date.accessioned.fl_str_mv |
2022-12-24T05:05:05Z |
dc.type.driver.fl_str_mv |
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0001-3765 |
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001149888 |
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http://hdl.handle.net/10183/253150 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Anais da Academia Brasileira de Ciências, Rio de Janeiro, RJ,. Vol. 92, Suppl. 1 (2020), e20181215, 13 p. |
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