A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/173408 |
Resumo: | The cattle tick Rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. Different species of ticks harbor a symbiont bacterium of the genus Coxiella. It was showed that a Coxiella endosymbiont from R. microplus (CERM) is a vertically transmitted mutualist symbiont, comprising 98% of the 16S rRNA sequences in both eggs and larvae. Sequencing of the bacterial genome revealed genes for biosynthetic pathways for several vitamins and key metabolic cofactors that may provide a nutritional complement to the tick host. The CERM was abundant in ovary and Malpighian tubule of fully engorged female. Tetracycline treatment of either the tick or the vertebrate host reduced levels of bacteria in progeny in 74% for eggs and 90% for larvae without major impact neither on the reproductive fitness of the adult female or on embryo development. However, CERM proved to be essential for the tick to reach the adult life stage, as under antibiotic treatment no tick was able to progress beyond the metanymph stage. Data presented here suggest that interference in the symbiotic CERM-R. microplus relationship may be useful to the development of alternative control methods, highlighting the interdependence between ticks and their endosymbionts. |
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Guizzo, Melina GarciaParizi, Luis FernandoNunes, Rodrigo DutraSchama, RenataAlbano, Rodolpho MattosTirloni, LucasOldiges, Daiane PatríciaVieira, Ricardo PilzOliveira, Wanderson Henrique CruzLeite, Milane de SouzaGonzales, Sergio AlbertoFarber, Marisa DianaMartins, OrlandoVaz Junior, Itabajara da SilvaOliveira, Pedro Lagerblad de2018-03-15T02:32:05Z20172045-2322http://hdl.handle.net/10183/173408001056056The cattle tick Rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. Different species of ticks harbor a symbiont bacterium of the genus Coxiella. It was showed that a Coxiella endosymbiont from R. microplus (CERM) is a vertically transmitted mutualist symbiont, comprising 98% of the 16S rRNA sequences in both eggs and larvae. Sequencing of the bacterial genome revealed genes for biosynthetic pathways for several vitamins and key metabolic cofactors that may provide a nutritional complement to the tick host. The CERM was abundant in ovary and Malpighian tubule of fully engorged female. Tetracycline treatment of either the tick or the vertebrate host reduced levels of bacteria in progeny in 74% for eggs and 90% for larvae without major impact neither on the reproductive fitness of the adult female or on embryo development. However, CERM proved to be essential for the tick to reach the adult life stage, as under antibiotic treatment no tick was able to progress beyond the metanymph stage. Data presented here suggest that interference in the symbiotic CERM-R. microplus relationship may be useful to the development of alternative control methods, highlighting the interdependence between ticks and their endosymbionts.application/pdfengScientific reports. London. Vol. 7 (2017), 17554 , [10] p.Parasitologia veterináriaMicrobiologia aplicadaRhipicephalus microplusCarrapato bovinoSimbioseGenômicaBacterias gram-negativasA Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplusEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001056056.pdf001056056.pdfTexto completo (inglês)application/pdf2237822http://www.lume.ufrgs.br/bitstream/10183/173408/1/001056056.pdfb3b2fee9e963cee2244c2678d6f5cd9aMD51TEXT001056056.pdf.txt001056056.pdf.txtExtracted Texttext/plain53586http://www.lume.ufrgs.br/bitstream/10183/173408/2/001056056.pdf.txt765085a7967316eb1b560d8c3f2ed147MD5210183/1734082018-03-16 02:31:50.863768oai:www.lume.ufrgs.br:10183/173408Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-03-16T05:31:50Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus |
title |
A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus |
spellingShingle |
A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus Guizzo, Melina Garcia Parasitologia veterinária Microbiologia aplicada Rhipicephalus microplus Carrapato bovino Simbiose Genômica Bacterias gram-negativas |
title_short |
A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus |
title_full |
A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus |
title_fullStr |
A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus |
title_full_unstemmed |
A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus |
title_sort |
A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus |
author |
Guizzo, Melina Garcia |
author_facet |
Guizzo, Melina Garcia Parizi, Luis Fernando Nunes, Rodrigo Dutra Schama, Renata Albano, Rodolpho Mattos Tirloni, Lucas Oldiges, Daiane Patrícia Vieira, Ricardo Pilz Oliveira, Wanderson Henrique Cruz Leite, Milane de Souza Gonzales, Sergio Alberto Farber, Marisa Diana Martins, Orlando Vaz Junior, Itabajara da Silva Oliveira, Pedro Lagerblad de |
author_role |
author |
author2 |
Parizi, Luis Fernando Nunes, Rodrigo Dutra Schama, Renata Albano, Rodolpho Mattos Tirloni, Lucas Oldiges, Daiane Patrícia Vieira, Ricardo Pilz Oliveira, Wanderson Henrique Cruz Leite, Milane de Souza Gonzales, Sergio Alberto Farber, Marisa Diana Martins, Orlando Vaz Junior, Itabajara da Silva Oliveira, Pedro Lagerblad de |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Guizzo, Melina Garcia Parizi, Luis Fernando Nunes, Rodrigo Dutra Schama, Renata Albano, Rodolpho Mattos Tirloni, Lucas Oldiges, Daiane Patrícia Vieira, Ricardo Pilz Oliveira, Wanderson Henrique Cruz Leite, Milane de Souza Gonzales, Sergio Alberto Farber, Marisa Diana Martins, Orlando Vaz Junior, Itabajara da Silva Oliveira, Pedro Lagerblad de |
dc.subject.por.fl_str_mv |
Parasitologia veterinária Microbiologia aplicada Rhipicephalus microplus Carrapato bovino Simbiose Genômica Bacterias gram-negativas |
topic |
Parasitologia veterinária Microbiologia aplicada Rhipicephalus microplus Carrapato bovino Simbiose Genômica Bacterias gram-negativas |
description |
The cattle tick Rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. Different species of ticks harbor a symbiont bacterium of the genus Coxiella. It was showed that a Coxiella endosymbiont from R. microplus (CERM) is a vertically transmitted mutualist symbiont, comprising 98% of the 16S rRNA sequences in both eggs and larvae. Sequencing of the bacterial genome revealed genes for biosynthetic pathways for several vitamins and key metabolic cofactors that may provide a nutritional complement to the tick host. The CERM was abundant in ovary and Malpighian tubule of fully engorged female. Tetracycline treatment of either the tick or the vertebrate host reduced levels of bacteria in progeny in 74% for eggs and 90% for larvae without major impact neither on the reproductive fitness of the adult female or on embryo development. However, CERM proved to be essential for the tick to reach the adult life stage, as under antibiotic treatment no tick was able to progress beyond the metanymph stage. Data presented here suggest that interference in the symbiotic CERM-R. microplus relationship may be useful to the development of alternative control methods, highlighting the interdependence between ticks and their endosymbionts. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2018-03-15T02:32:05Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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http://hdl.handle.net/10183/173408 |
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2045-2322 |
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001056056 |
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2045-2322 001056056 |
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http://hdl.handle.net/10183/173408 |
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eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Scientific reports. London. Vol. 7 (2017), 17554 , [10] p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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