Effect of electrospun Phb and Hap-Phb composite scaffolds characteristics on mesenchymal stem cell growth viability
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/173745 |
Resumo: | Cell-cell and material-cell interactions have been identified as key factors modulating cellular differentiation and extracellular matrix (ECM) production. Stem cells, in vivo and in vitro cells cultures, respond to biological signals received by their microenvironment surroundings. Via this stimulation, cells can secrete many factors to modulate their microenvironment and activate progenitor cells. This induction results in an increase in the expression of ECM proteins that are associated with cellular adhesion and proliferation and matrix mineralization. Based on the hypothesis that nanostructures can influence steam cell signalization, a long-standing goal has been fabricating a scaffold of polyhydroxybutyrate (PHB) with hydroxyapatite (HAp) incorporated to imitate a bone ECM. Using the electrospinning technique, it is possible to fabricate micro and nanostructured scaffolds. Structural characterization and viability evaluation of these scaffolds, such as adhesion and cellular proliferation, have demonstrated that scaffolds exhibit biocompatibility. Scaffolds containing HAp produce a mineralized matrix, which shows that mesenchymal stem cells differentiate to the osteogenic lineage. These scaffolds have accordingly been shown to have bone regeneration applicability in sites that are not subject to excessive loads. |
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Viezzer, ChristianForte, Maria Madalena de CamargoBerutti, Felipe AmorimAlves, Annelise KoppBergmann, Carlos Perez2018-03-23T02:26:24Z20172576-4519http://hdl.handle.net/10183/173745001060477Cell-cell and material-cell interactions have been identified as key factors modulating cellular differentiation and extracellular matrix (ECM) production. Stem cells, in vivo and in vitro cells cultures, respond to biological signals received by their microenvironment surroundings. Via this stimulation, cells can secrete many factors to modulate their microenvironment and activate progenitor cells. This induction results in an increase in the expression of ECM proteins that are associated with cellular adhesion and proliferation and matrix mineralization. Based on the hypothesis that nanostructures can influence steam cell signalization, a long-standing goal has been fabricating a scaffold of polyhydroxybutyrate (PHB) with hydroxyapatite (HAp) incorporated to imitate a bone ECM. Using the electrospinning technique, it is possible to fabricate micro and nanostructured scaffolds. Structural characterization and viability evaluation of these scaffolds, such as adhesion and cellular proliferation, have demonstrated that scaffolds exhibit biocompatibility. Scaffolds containing HAp produce a mineralized matrix, which shows that mesenchymal stem cells differentiate to the osteogenic lineage. These scaffolds have accordingly been shown to have bone regeneration applicability in sites that are not subject to excessive loads.application/pdfengMOJ Applied bionics and biomechanics [recurso eletrônico]. Edmond. vol. 1, no. 6 (2017), art. 00035, 8 p.Engenharia de tecidosOsso e ossosHidroxiapatitaPolihidroxibutiratoCélulas-troncoNanoestruturasBone tissue engineeringNanostructuresHydroxyapatitePolyhydroxybutyrateStem cellsMineralized matrixEffect of electrospun Phb and Hap-Phb composite scaffolds characteristics on mesenchymal stem cell growth viabilityEffect of electrospun polyhydroxybutyrate and hydroxyapatite-polyhydroxybutyrate composite scaffolds characteristics on mesenchymal stem cell growth viability Estrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001060477.pdf001060477.pdfTexto completo (inglês)application/pdf1834280http://www.lume.ufrgs.br/bitstream/10183/173745/1/001060477.pdfebdb4a320d4362223ed07239ac76f7ebMD51TEXT001060477.pdf.txt001060477.pdf.txtExtracted Texttext/plain36808http://www.lume.ufrgs.br/bitstream/10183/173745/2/001060477.pdf.txt72d34396fed766725363238605817520MD52THUMBNAIL001060477.pdf.jpg001060477.pdf.jpgGenerated Thumbnailimage/jpeg2091http://www.lume.ufrgs.br/bitstream/10183/173745/3/001060477.pdf.jpg01df34a78529778fc9d82ac4e51f9930MD5310183/1737452018-10-05 07:29:06.881oai:www.lume.ufrgs.br:10183/173745Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-05T10:29:06Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Effect of electrospun Phb and Hap-Phb composite scaffolds characteristics on mesenchymal stem cell growth viability |
dc.title.alternative.pt_BR.fl_str_mv |
Effect of electrospun polyhydroxybutyrate and hydroxyapatite-polyhydroxybutyrate composite scaffolds characteristics on mesenchymal stem cell growth viability |
title |
Effect of electrospun Phb and Hap-Phb composite scaffolds characteristics on mesenchymal stem cell growth viability |
spellingShingle |
Effect of electrospun Phb and Hap-Phb composite scaffolds characteristics on mesenchymal stem cell growth viability Viezzer, Christian Engenharia de tecidos Osso e ossos Hidroxiapatita Polihidroxibutirato Células-tronco Nanoestruturas Bone tissue engineering Nanostructures Hydroxyapatite Polyhydroxybutyrate Stem cells Mineralized matrix |
title_short |
Effect of electrospun Phb and Hap-Phb composite scaffolds characteristics on mesenchymal stem cell growth viability |
title_full |
Effect of electrospun Phb and Hap-Phb composite scaffolds characteristics on mesenchymal stem cell growth viability |
title_fullStr |
Effect of electrospun Phb and Hap-Phb composite scaffolds characteristics on mesenchymal stem cell growth viability |
title_full_unstemmed |
Effect of electrospun Phb and Hap-Phb composite scaffolds characteristics on mesenchymal stem cell growth viability |
title_sort |
Effect of electrospun Phb and Hap-Phb composite scaffolds characteristics on mesenchymal stem cell growth viability |
author |
Viezzer, Christian |
author_facet |
Viezzer, Christian Forte, Maria Madalena de Camargo Berutti, Felipe Amorim Alves, Annelise Kopp Bergmann, Carlos Perez |
author_role |
author |
author2 |
Forte, Maria Madalena de Camargo Berutti, Felipe Amorim Alves, Annelise Kopp Bergmann, Carlos Perez |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Viezzer, Christian Forte, Maria Madalena de Camargo Berutti, Felipe Amorim Alves, Annelise Kopp Bergmann, Carlos Perez |
dc.subject.por.fl_str_mv |
Engenharia de tecidos Osso e ossos Hidroxiapatita Polihidroxibutirato Células-tronco Nanoestruturas |
topic |
Engenharia de tecidos Osso e ossos Hidroxiapatita Polihidroxibutirato Células-tronco Nanoestruturas Bone tissue engineering Nanostructures Hydroxyapatite Polyhydroxybutyrate Stem cells Mineralized matrix |
dc.subject.eng.fl_str_mv |
Bone tissue engineering Nanostructures Hydroxyapatite Polyhydroxybutyrate Stem cells Mineralized matrix |
description |
Cell-cell and material-cell interactions have been identified as key factors modulating cellular differentiation and extracellular matrix (ECM) production. Stem cells, in vivo and in vitro cells cultures, respond to biological signals received by their microenvironment surroundings. Via this stimulation, cells can secrete many factors to modulate their microenvironment and activate progenitor cells. This induction results in an increase in the expression of ECM proteins that are associated with cellular adhesion and proliferation and matrix mineralization. Based on the hypothesis that nanostructures can influence steam cell signalization, a long-standing goal has been fabricating a scaffold of polyhydroxybutyrate (PHB) with hydroxyapatite (HAp) incorporated to imitate a bone ECM. Using the electrospinning technique, it is possible to fabricate micro and nanostructured scaffolds. Structural characterization and viability evaluation of these scaffolds, such as adhesion and cellular proliferation, have demonstrated that scaffolds exhibit biocompatibility. Scaffolds containing HAp produce a mineralized matrix, which shows that mesenchymal stem cells differentiate to the osteogenic lineage. These scaffolds have accordingly been shown to have bone regeneration applicability in sites that are not subject to excessive loads. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2018-03-23T02:26:24Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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2576-4519 |
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001060477 |
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http://hdl.handle.net/10183/173745 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
MOJ Applied bionics and biomechanics [recurso eletrônico]. Edmond. vol. 1, no. 6 (2017), art. 00035, 8 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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