Neonatal morphine exposure and maternal deprivation alter nociceptive response and central biomarkers’ levels throughout the life of rats
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/216736 |
Resumo: | In the present study, we investigated the effect of repeated neonatal morphine exposure and/or maternal deprivation(MD) on the nociceptive response and central biomarkers’ BDNF, IL-1β, and IL-4 levels at postnatal days 16(PND16), 30(PND30), and 60(PND60). At birth, the litters were standardized to contain 8 pups/dam (n = 58). From PND1 to PND10, the pups of the deprived groups were separated daily from their mothers for 3 h and divided into 5 groups: control(C), saline(S), morphine(M), deprived-saline(DS), and deprived-morphine (DM). The pups received subcutaneous injections of saline/morphine (5 μg) in the mid-scapular area between PND8 and PND14. Nociceptive responses were assessed by hot plate(HP) and tail-flick(TFL) tests and biomarker levels by ELISA. Thermal hyperalgesia(HP) was found in all assessments for the M, DS, and DM groups, and a decrease in nociceptive threshold(TFL) was found in the DS group at PND16; M and DM groups at PND30; and M, DS, and DM groups at PND60. There were interactions between treatment/deprivation/timepoint in all central biomarkers’ levels. The current study indicates that neonatal exposure to morphine and MD, which occurs in the pediatric ICU, can alter the nociceptive and neuroinflammatory responses. |
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Oliveira, Carla deStröher, RobertaScarabelot, Vanessa LealVercelino, RafaelSilva, Lisiane Santos daRegner, Gabriela GregorySouza, Andressa deSilveira, Natália P.Torres, Iraci Lucena da SilvaCaumo, Wolnei2020-12-19T04:19:37Z20201872-7972http://hdl.handle.net/10183/216736001119815In the present study, we investigated the effect of repeated neonatal morphine exposure and/or maternal deprivation(MD) on the nociceptive response and central biomarkers’ BDNF, IL-1β, and IL-4 levels at postnatal days 16(PND16), 30(PND30), and 60(PND60). At birth, the litters were standardized to contain 8 pups/dam (n = 58). From PND1 to PND10, the pups of the deprived groups were separated daily from their mothers for 3 h and divided into 5 groups: control(C), saline(S), morphine(M), deprived-saline(DS), and deprived-morphine (DM). The pups received subcutaneous injections of saline/morphine (5 μg) in the mid-scapular area between PND8 and PND14. Nociceptive responses were assessed by hot plate(HP) and tail-flick(TFL) tests and biomarker levels by ELISA. Thermal hyperalgesia(HP) was found in all assessments for the M, DS, and DM groups, and a decrease in nociceptive threshold(TFL) was found in the DS group at PND16; M and DM groups at PND30; and M, DS, and DM groups at PND60. There were interactions between treatment/deprivation/timepoint in all central biomarkers’ levels. The current study indicates that neonatal exposure to morphine and MD, which occurs in the pediatric ICU, can alter the nociceptive and neuroinflammatory responses.application/pdfengNeuroscience letters. Limerick. Vol. 738 (2020), 135350, 8 p.Analgesicos opióidesPrivação maternaNociceptividadeModelos animaisOpioidMaternal-separationNeonate ratsNociceptionNeonatal morphine exposure and maternal deprivation alter nociceptive response and central biomarkers’ levels throughout the life of ratsEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001119815.pdf.txt001119815.pdf.txtExtracted Texttext/plain37818http://www.lume.ufrgs.br/bitstream/10183/216736/2/001119815.pdf.txtbd966f32c58b2a9902986d64901b4835MD52ORIGINAL001119815.pdfTexto completo (inglês)application/pdf2734387http://www.lume.ufrgs.br/bitstream/10183/216736/1/001119815.pdf16368ab731f054f96db0fc0e1b0c98caMD5110183/2167362021-08-18 04:40:34.217983oai:www.lume.ufrgs.br:10183/216736Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-08-18T07:40:34Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Neonatal morphine exposure and maternal deprivation alter nociceptive response and central biomarkers’ levels throughout the life of rats |
title |
Neonatal morphine exposure and maternal deprivation alter nociceptive response and central biomarkers’ levels throughout the life of rats |
spellingShingle |
Neonatal morphine exposure and maternal deprivation alter nociceptive response and central biomarkers’ levels throughout the life of rats Oliveira, Carla de Analgesicos opióides Privação materna Nociceptividade Modelos animais Opioid Maternal-separation Neonate rats Nociception |
title_short |
Neonatal morphine exposure and maternal deprivation alter nociceptive response and central biomarkers’ levels throughout the life of rats |
title_full |
Neonatal morphine exposure and maternal deprivation alter nociceptive response and central biomarkers’ levels throughout the life of rats |
title_fullStr |
Neonatal morphine exposure and maternal deprivation alter nociceptive response and central biomarkers’ levels throughout the life of rats |
title_full_unstemmed |
Neonatal morphine exposure and maternal deprivation alter nociceptive response and central biomarkers’ levels throughout the life of rats |
title_sort |
Neonatal morphine exposure and maternal deprivation alter nociceptive response and central biomarkers’ levels throughout the life of rats |
author |
Oliveira, Carla de |
author_facet |
Oliveira, Carla de Ströher, Roberta Scarabelot, Vanessa Leal Vercelino, Rafael Silva, Lisiane Santos da Regner, Gabriela Gregory Souza, Andressa de Silveira, Natália P. Torres, Iraci Lucena da Silva Caumo, Wolnei |
author_role |
author |
author2 |
Ströher, Roberta Scarabelot, Vanessa Leal Vercelino, Rafael Silva, Lisiane Santos da Regner, Gabriela Gregory Souza, Andressa de Silveira, Natália P. Torres, Iraci Lucena da Silva Caumo, Wolnei |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Carla de Ströher, Roberta Scarabelot, Vanessa Leal Vercelino, Rafael Silva, Lisiane Santos da Regner, Gabriela Gregory Souza, Andressa de Silveira, Natália P. Torres, Iraci Lucena da Silva Caumo, Wolnei |
dc.subject.por.fl_str_mv |
Analgesicos opióides Privação materna Nociceptividade Modelos animais |
topic |
Analgesicos opióides Privação materna Nociceptividade Modelos animais Opioid Maternal-separation Neonate rats Nociception |
dc.subject.eng.fl_str_mv |
Opioid Maternal-separation Neonate rats Nociception |
description |
In the present study, we investigated the effect of repeated neonatal morphine exposure and/or maternal deprivation(MD) on the nociceptive response and central biomarkers’ BDNF, IL-1β, and IL-4 levels at postnatal days 16(PND16), 30(PND30), and 60(PND60). At birth, the litters were standardized to contain 8 pups/dam (n = 58). From PND1 to PND10, the pups of the deprived groups were separated daily from their mothers for 3 h and divided into 5 groups: control(C), saline(S), morphine(M), deprived-saline(DS), and deprived-morphine (DM). The pups received subcutaneous injections of saline/morphine (5 μg) in the mid-scapular area between PND8 and PND14. Nociceptive responses were assessed by hot plate(HP) and tail-flick(TFL) tests and biomarker levels by ELISA. Thermal hyperalgesia(HP) was found in all assessments for the M, DS, and DM groups, and a decrease in nociceptive threshold(TFL) was found in the DS group at PND16; M and DM groups at PND30; and M, DS, and DM groups at PND60. There were interactions between treatment/deprivation/timepoint in all central biomarkers’ levels. The current study indicates that neonatal exposure to morphine and MD, which occurs in the pediatric ICU, can alter the nociceptive and neuroinflammatory responses. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-12-19T04:19:37Z |
dc.date.issued.fl_str_mv |
2020 |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
format |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/216736 |
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1872-7972 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001119815 |
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1872-7972 001119815 |
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http://hdl.handle.net/10183/216736 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Neuroscience letters. Limerick. Vol. 738 (2020), 135350, 8 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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