A potential source for cellulolytic enzyme discovery and environmental aspects revealed through metagenomics of brazilian mangroves
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/110156 |
Resumo: | The mangroves are among the most productive and biologically important environments. The possible presence of cellulolytic enzymes and microorganisms useful for biomass degradation as well as taxonomic and functional aspects of two Brazilian mangroves were evaluated using cultivation and metagenomic approaches. From a total of 296 microorganisms with visual differences in colony morphology and growth (including bacteria, yeast and filamentous fungus), 179 (60.5%) and 117 (39.5%) were isolated from the Rio de Janeiro (RJ) and Bahia (BA) samples, respectively. RJ metagenome showed the higher number of microbial isolates, which is consistent with its most conserved state and higher diversity. The metagenomic sequencing data showed similar predominant bacterial phyla in the BA and RJ mangroves with an abundance of Proteobacteria (57.8% and 44.6%), Firmicutes (11% and 12.3%) and Actinobacteria (8.4% and 7.5%). A higher number of enzymes involved in the degradation of polycyclic aromatic compounds were found in the BA mangrove. Specific sequences involved in the cellulolytic degradation, belonging to cellulases, hemicellulases, carbohydrate binding domains, dockerins and cohesins were identified, and it was possible to isolate cultivable fungi and bacteria related to biomass decomposition and with potential applications for the production of biofuels. These results showed that the mangroves possess all fundamental molecular tools required for building the cellulosome, which is required for the efficient degradation of cellulose material and sugar release. |
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Thompson, Claudia ElizabethSilva, Walter Orlando Beys daSanti, LucéliaOliveira, Markus BergerVainstein, Marilene HenningGuimaraes, Jorge AlmeidaVasconcelos, Ana Tereza Ribeiro de2015-02-14T02:19:48Z20132191-0855http://hdl.handle.net/10183/110156000931565The mangroves are among the most productive and biologically important environments. The possible presence of cellulolytic enzymes and microorganisms useful for biomass degradation as well as taxonomic and functional aspects of two Brazilian mangroves were evaluated using cultivation and metagenomic approaches. From a total of 296 microorganisms with visual differences in colony morphology and growth (including bacteria, yeast and filamentous fungus), 179 (60.5%) and 117 (39.5%) were isolated from the Rio de Janeiro (RJ) and Bahia (BA) samples, respectively. RJ metagenome showed the higher number of microbial isolates, which is consistent with its most conserved state and higher diversity. The metagenomic sequencing data showed similar predominant bacterial phyla in the BA and RJ mangroves with an abundance of Proteobacteria (57.8% and 44.6%), Firmicutes (11% and 12.3%) and Actinobacteria (8.4% and 7.5%). A higher number of enzymes involved in the degradation of polycyclic aromatic compounds were found in the BA mangrove. Specific sequences involved in the cellulolytic degradation, belonging to cellulases, hemicellulases, carbohydrate binding domains, dockerins and cohesins were identified, and it was possible to isolate cultivable fungi and bacteria related to biomass decomposition and with potential applications for the production of biofuels. These results showed that the mangroves possess all fundamental molecular tools required for building the cellulosome, which is required for the efficient degradation of cellulose material and sugar release.application/pdfengAMB Express. Heidelberg, GW. Vol. 3, no. 1 (2013), p. 65-83Mangues : BrasilCelulaseBiomassaBrazilian mangroveCellulaseMetagenomicsBiomass decompositionBacterial diversityA potential source for cellulolytic enzyme discovery and environmental aspects revealed through metagenomics of brazilian mangrovesEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000931565.pdf.txt000931565.pdf.txtExtracted Texttext/plain80476http://www.lume.ufrgs.br/bitstream/10183/110156/2/000931565.pdf.txtf2773d79eb58228004cb77537467909fMD52ORIGINAL000931565.pdf000931565.pdfTexto completo (inglês)application/pdf2609252http://www.lume.ufrgs.br/bitstream/10183/110156/1/000931565.pdf4ba63a0fbc086f90723112429c99179eMD51THUMBNAIL000931565.pdf.jpg000931565.pdf.jpgGenerated Thumbnailimage/jpeg1995http://www.lume.ufrgs.br/bitstream/10183/110156/3/000931565.pdf.jpge8dae0fdf4c0766ab1367fa97f415de0MD5310183/1101562018-10-23 09:19:28.481oai:www.lume.ufrgs.br:10183/110156Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-23T12:19:28Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
A potential source for cellulolytic enzyme discovery and environmental aspects revealed through metagenomics of brazilian mangroves |
title |
A potential source for cellulolytic enzyme discovery and environmental aspects revealed through metagenomics of brazilian mangroves |
spellingShingle |
A potential source for cellulolytic enzyme discovery and environmental aspects revealed through metagenomics of brazilian mangroves Thompson, Claudia Elizabeth Mangues : Brasil Celulase Biomassa Brazilian mangrove Cellulase Metagenomics Biomass decomposition Bacterial diversity |
title_short |
A potential source for cellulolytic enzyme discovery and environmental aspects revealed through metagenomics of brazilian mangroves |
title_full |
A potential source for cellulolytic enzyme discovery and environmental aspects revealed through metagenomics of brazilian mangroves |
title_fullStr |
A potential source for cellulolytic enzyme discovery and environmental aspects revealed through metagenomics of brazilian mangroves |
title_full_unstemmed |
A potential source for cellulolytic enzyme discovery and environmental aspects revealed through metagenomics of brazilian mangroves |
title_sort |
A potential source for cellulolytic enzyme discovery and environmental aspects revealed through metagenomics of brazilian mangroves |
author |
Thompson, Claudia Elizabeth |
author_facet |
Thompson, Claudia Elizabeth Silva, Walter Orlando Beys da Santi, Lucélia Oliveira, Markus Berger Vainstein, Marilene Henning Guimaraes, Jorge Almeida Vasconcelos, Ana Tereza Ribeiro de |
author_role |
author |
author2 |
Silva, Walter Orlando Beys da Santi, Lucélia Oliveira, Markus Berger Vainstein, Marilene Henning Guimaraes, Jorge Almeida Vasconcelos, Ana Tereza Ribeiro de |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Thompson, Claudia Elizabeth Silva, Walter Orlando Beys da Santi, Lucélia Oliveira, Markus Berger Vainstein, Marilene Henning Guimaraes, Jorge Almeida Vasconcelos, Ana Tereza Ribeiro de |
dc.subject.por.fl_str_mv |
Mangues : Brasil Celulase Biomassa |
topic |
Mangues : Brasil Celulase Biomassa Brazilian mangrove Cellulase Metagenomics Biomass decomposition Bacterial diversity |
dc.subject.eng.fl_str_mv |
Brazilian mangrove Cellulase Metagenomics Biomass decomposition Bacterial diversity |
description |
The mangroves are among the most productive and biologically important environments. The possible presence of cellulolytic enzymes and microorganisms useful for biomass degradation as well as taxonomic and functional aspects of two Brazilian mangroves were evaluated using cultivation and metagenomic approaches. From a total of 296 microorganisms with visual differences in colony morphology and growth (including bacteria, yeast and filamentous fungus), 179 (60.5%) and 117 (39.5%) were isolated from the Rio de Janeiro (RJ) and Bahia (BA) samples, respectively. RJ metagenome showed the higher number of microbial isolates, which is consistent with its most conserved state and higher diversity. The metagenomic sequencing data showed similar predominant bacterial phyla in the BA and RJ mangroves with an abundance of Proteobacteria (57.8% and 44.6%), Firmicutes (11% and 12.3%) and Actinobacteria (8.4% and 7.5%). A higher number of enzymes involved in the degradation of polycyclic aromatic compounds were found in the BA mangrove. Specific sequences involved in the cellulolytic degradation, belonging to cellulases, hemicellulases, carbohydrate binding domains, dockerins and cohesins were identified, and it was possible to isolate cultivable fungi and bacteria related to biomass decomposition and with potential applications for the production of biofuels. These results showed that the mangroves possess all fundamental molecular tools required for building the cellulosome, which is required for the efficient degradation of cellulose material and sugar release. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2015-02-14T02:19:48Z |
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http://hdl.handle.net/10183/110156 |
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2191-0855 |
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000931565 |
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http://hdl.handle.net/10183/110156 |
dc.language.iso.fl_str_mv |
eng |
language |
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dc.relation.ispartof.pt_BR.fl_str_mv |
AMB Express. Heidelberg, GW. Vol. 3, no. 1 (2013), p. 65-83 |
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openAccess |
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