Scrapie diagnosis in a goat and four Santa Inês sheep from the same herd in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/263558 |
Resumo: | Scrapie is a fatal and progressive transmissible spongiform encephalopathy (TSE) of natural occurrence in sheep and goats. The suspicion of scrapie may be based on clinical signs; however, the detection of pathological features of the prionic protein (PrP) in target tissues is necessary to diagnose the disease. The presence of an abnormal protein form (PrPSc) in lymphoreticular and nervous tissues is an important characteristic in diagnosis. This paper reports a case of scrapie in a flock of 55 Suffolk crossbred sheep, 19 Santa Inês sheep and 21 goats in the Mato Grosso state, midwestern Brazil. The animals were euthanized after the confirmation of a scrapie case with clinical signs in a Suffolk sheep in the same farm. Samples of brainstem at the level of the obex and lymphoid issues like palatine tonsils, mesenteric lymph nodes, third eyelid fixed in formalin 10% were processed for histological examination. Histological examination with hematoxylin and eosin did not show any microscopic changes in samples. Immunohistochemistry (IHC) examination to detect anti-prion PrPSc was performed in lymphoid tissues. Scrapie diagnosis was confirmed based on IHC positive results for PrPSc in lymphoid tissues of a crossbreed goat and four Santa Inês sheep, without any clinical scrapie signs. IHC showed positive staining in at least three lymphoid germinal centers in goat mesenteric lymph node, palatine tonsil, and third eyelid samples. The mesenteric lymph node, and tonsil samples of all sheep showed positive immunostaining, and only one sheep showed positive staining in lymphoid follicles in the third eyelid. Scrapie diagnosis using IHC in fixed samples of lymphoreticular tissue is technically feasible to detect the disease in both goats and sheep, as a form of pre-clinical diagnosis. The results indicate that the herd was infected by a sheep coming from another herd where scrapie had been diagnosed before. |
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Leal, Juliano de SouzaCorrea, Gabriel Laizola FrainerBoos, Gisele SilvaBianchi, Matheus ViezzerBoabaid, Fabiana M.Lopes, Rui Fernando FelixDriemeier, David2023-08-16T03:30:35Z20150102-0935http://hdl.handle.net/10183/263558000984880Scrapie is a fatal and progressive transmissible spongiform encephalopathy (TSE) of natural occurrence in sheep and goats. The suspicion of scrapie may be based on clinical signs; however, the detection of pathological features of the prionic protein (PrP) in target tissues is necessary to diagnose the disease. The presence of an abnormal protein form (PrPSc) in lymphoreticular and nervous tissues is an important characteristic in diagnosis. This paper reports a case of scrapie in a flock of 55 Suffolk crossbred sheep, 19 Santa Inês sheep and 21 goats in the Mato Grosso state, midwestern Brazil. The animals were euthanized after the confirmation of a scrapie case with clinical signs in a Suffolk sheep in the same farm. Samples of brainstem at the level of the obex and lymphoid issues like palatine tonsils, mesenteric lymph nodes, third eyelid fixed in formalin 10% were processed for histological examination. Histological examination with hematoxylin and eosin did not show any microscopic changes in samples. Immunohistochemistry (IHC) examination to detect anti-prion PrPSc was performed in lymphoid tissues. Scrapie diagnosis was confirmed based on IHC positive results for PrPSc in lymphoid tissues of a crossbreed goat and four Santa Inês sheep, without any clinical scrapie signs. IHC showed positive staining in at least three lymphoid germinal centers in goat mesenteric lymph node, palatine tonsil, and third eyelid samples. The mesenteric lymph node, and tonsil samples of all sheep showed positive immunostaining, and only one sheep showed positive staining in lymphoid follicles in the third eyelid. Scrapie diagnosis using IHC in fixed samples of lymphoreticular tissue is technically feasible to detect the disease in both goats and sheep, as a form of pre-clinical diagnosis. The results indicate that the herd was infected by a sheep coming from another herd where scrapie had been diagnosed before.Scrapie é uma encefalopatia espongiforme transmissível (EET) progressiva e fatal de ocorrência natural em ovinos e caprinos. A suspeita de scrapie é baseada nos sinais clínicos, porém a manifestação patológica da proteína priônica (PrP) nos tecidos-alvo é necessária para a confirmação da doença. A presença de uma forma anormal da proteína (PrPSc) em tecido linforreticular e tecido nervoso constitui uma característica importante para o diagnóstico. Este trabalho é o relato de um foco de scrapie ocorrido em rebanho com 55 ovinos mistos Suffolk, 21 caprinos e 19 ovinos Santa Inês, na região Centro-Oeste do Brasil. Os animais foram eutanasiados após a confirmação de um caso de scrapie com sinais clínicos em um ovino Suffolk nessa propriedade. Amostras de tronco cerebral na altura do obex e tecidos linfoides, que incluíram tonsilas, linfonodos mesentéricos e tecido linfoide da terceira pálpebra foram processados para exame histológico. O exame histológico utilizando a coloração de hematoxilina e eosina não revelou a presença de alterações microscópicas nos tecidos examinados. O diagnóstico de scrapie foi confirmado com base nos resultados positivos de imuno-histoquímica (IHQ) para PrPSc nos tecidos linfoides de um caprino sem raça definida e quatro ovinos da raça Santa Inês, sem sinais clínicos de scrapie. A IHQ apresentou marcação positiva em pelo menos três centros linfoides na tonsila, terceira pálpebra e linfonodo mesentérico do caprino. Em todos os ovinos, a IHQ revelou marcação positiva nos folículos linfoides da tonsila palatínica e linfonodo mesentérico; a marcação positiva nos folículos linfoides da terceira pálpebra só foi observada em um dos ovinos. Este trabalho demonstra a importância da utilização de tecido linforreticular para o diagnóstico pré-clínico de scrapie através de IHQ e é tecnicamente viável em ovinos e caprinos. Os resultados indicam que os animais foram contaminados por um ovino oriundo de um rebanho que apresentava casos clínicos de scrapie.application/pdfengArquivo brasileiro de medicina veterinária e zootecnia. Belo Horizonte. Vol. 67, no. 6 (nov./dec. 2015), p. 1625-1629ScrapieImuno-histoquímicaTecido linfóideScrapiePrionDiseases of small ruminantsImmunohistochemistryLymphoid tissuesScrapie diagnosis in a goat and four Santa Inês sheep from the same herd in Brazilinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000984880.pdf.txt000984880.pdf.txtExtracted Texttext/plain18081http://www.lume.ufrgs.br/bitstream/10183/263558/2/000984880.pdf.txt2434dc0e0fd13db7ae3e9fd713f7e858MD52ORIGINAL000984880.pdfTexto completo (inglês)application/pdf405754http://www.lume.ufrgs.br/bitstream/10183/263558/1/000984880.pdf1611945e0672e28da6ef35e75457eaddMD5110183/2635582023-08-17 03:34:06.294349oai:www.lume.ufrgs.br:10183/263558Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-08-17T06:34:06Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Scrapie diagnosis in a goat and four Santa Inês sheep from the same herd in Brazil |
title |
Scrapie diagnosis in a goat and four Santa Inês sheep from the same herd in Brazil |
spellingShingle |
Scrapie diagnosis in a goat and four Santa Inês sheep from the same herd in Brazil Leal, Juliano de Souza Scrapie Imuno-histoquímica Tecido linfóide Scrapie Prion Diseases of small ruminants Immunohistochemistry Lymphoid tissues |
title_short |
Scrapie diagnosis in a goat and four Santa Inês sheep from the same herd in Brazil |
title_full |
Scrapie diagnosis in a goat and four Santa Inês sheep from the same herd in Brazil |
title_fullStr |
Scrapie diagnosis in a goat and four Santa Inês sheep from the same herd in Brazil |
title_full_unstemmed |
Scrapie diagnosis in a goat and four Santa Inês sheep from the same herd in Brazil |
title_sort |
Scrapie diagnosis in a goat and four Santa Inês sheep from the same herd in Brazil |
author |
Leal, Juliano de Souza |
author_facet |
Leal, Juliano de Souza Correa, Gabriel Laizola Frainer Boos, Gisele Silva Bianchi, Matheus Viezzer Boabaid, Fabiana M. Lopes, Rui Fernando Felix Driemeier, David |
author_role |
author |
author2 |
Correa, Gabriel Laizola Frainer Boos, Gisele Silva Bianchi, Matheus Viezzer Boabaid, Fabiana M. Lopes, Rui Fernando Felix Driemeier, David |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Leal, Juliano de Souza Correa, Gabriel Laizola Frainer Boos, Gisele Silva Bianchi, Matheus Viezzer Boabaid, Fabiana M. Lopes, Rui Fernando Felix Driemeier, David |
dc.subject.por.fl_str_mv |
Scrapie Imuno-histoquímica Tecido linfóide |
topic |
Scrapie Imuno-histoquímica Tecido linfóide Scrapie Prion Diseases of small ruminants Immunohistochemistry Lymphoid tissues |
dc.subject.eng.fl_str_mv |
Scrapie Prion Diseases of small ruminants Immunohistochemistry Lymphoid tissues |
description |
Scrapie is a fatal and progressive transmissible spongiform encephalopathy (TSE) of natural occurrence in sheep and goats. The suspicion of scrapie may be based on clinical signs; however, the detection of pathological features of the prionic protein (PrP) in target tissues is necessary to diagnose the disease. The presence of an abnormal protein form (PrPSc) in lymphoreticular and nervous tissues is an important characteristic in diagnosis. This paper reports a case of scrapie in a flock of 55 Suffolk crossbred sheep, 19 Santa Inês sheep and 21 goats in the Mato Grosso state, midwestern Brazil. The animals were euthanized after the confirmation of a scrapie case with clinical signs in a Suffolk sheep in the same farm. Samples of brainstem at the level of the obex and lymphoid issues like palatine tonsils, mesenteric lymph nodes, third eyelid fixed in formalin 10% were processed for histological examination. Histological examination with hematoxylin and eosin did not show any microscopic changes in samples. Immunohistochemistry (IHC) examination to detect anti-prion PrPSc was performed in lymphoid tissues. Scrapie diagnosis was confirmed based on IHC positive results for PrPSc in lymphoid tissues of a crossbreed goat and four Santa Inês sheep, without any clinical scrapie signs. IHC showed positive staining in at least three lymphoid germinal centers in goat mesenteric lymph node, palatine tonsil, and third eyelid samples. The mesenteric lymph node, and tonsil samples of all sheep showed positive immunostaining, and only one sheep showed positive staining in lymphoid follicles in the third eyelid. Scrapie diagnosis using IHC in fixed samples of lymphoreticular tissue is technically feasible to detect the disease in both goats and sheep, as a form of pre-clinical diagnosis. The results indicate that the herd was infected by a sheep coming from another herd where scrapie had been diagnosed before. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
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2023-08-16T03:30:35Z |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/263558 |
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0102-0935 |
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000984880 |
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language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Arquivo brasileiro de medicina veterinária e zootecnia. Belo Horizonte. Vol. 67, no. 6 (nov./dec. 2015), p. 1625-1629 |
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