Genotypes and pathogenicity of cellulitis isolates reveal traits that modulate APEC virulence
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/115264 |
Resumo: | We characterized 144 Escherichia coli isolates from severe cellulitis lesions in broiler chickens from South Brazil. Analysis of susceptibility to 15 antimicrobials revealed frequencies of resistance of less than 30% for most antimicrobials except tetracycline (70%) and sulphonamides (60%). The genotyping of 34 virulence-associated genes revealed that all the isolates harbored virulence factors related to adhesion, iron acquisition and serum resistance, which are characteristic of the avian pathogenic E. coli (APEC) pathotype. ColV plasmid-associated genes (cvi/cva, iroN, iss, iucD, sitD, traT, tsh) were especially frequent among the isolates (from 66.6% to 89.6%). According to the Clermont method of ECOR phylogenetic typing, isolates belonged to group D (47.2%), to group A (27.8%), to group B2 (17.4%) and to group B1 (7.6%); the group B2 isolates contained the highest number of virulence-associated genes. Clonal relationship analysis using the ARDRA method revealed a similarity level of 57% or higher among isolates, but no endemic clone. The virulence of the isolates was confirmed in vivo in one-day-old chicks. Most isolates (72.9%) killed all infected chicks within 7 days, and 65 isolates (38.1%) killed most of them within 24 hours. In order to analyze differences in virulence among the APEC isolates, we created a pathogenicity score by combining the times of death with the clinical symptoms noted. By looking for significant associations between the presence of virulence-associated genes and the pathogenicity score, we found that the presence of genes for invasins ibeA and gimB and for group II capsule KpsMTII increased virulence, while the presence of pic decreased virulence. The fact that ibeA, gimB and KpsMTII are characteristic of neonatal meningitis E. coli (NMEC) suggests that genes of NMEC in APEC increase virulence of strains. |
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Barbieri, Nicolle LimaOliveira, Aline Luísa deTejkowski, Thiago MoreiraPavanelo, Daniel BrisottoRocha, Débora AssumpçãoMatter, Leticia BeatrizCallegari-Jacques, Sidia MariaBrito, Benito Guimarães deHorn, Fabiana2015-04-14T01:57:40Z20131932-6203http://hdl.handle.net/10183/115264000897978We characterized 144 Escherichia coli isolates from severe cellulitis lesions in broiler chickens from South Brazil. Analysis of susceptibility to 15 antimicrobials revealed frequencies of resistance of less than 30% for most antimicrobials except tetracycline (70%) and sulphonamides (60%). The genotyping of 34 virulence-associated genes revealed that all the isolates harbored virulence factors related to adhesion, iron acquisition and serum resistance, which are characteristic of the avian pathogenic E. coli (APEC) pathotype. ColV plasmid-associated genes (cvi/cva, iroN, iss, iucD, sitD, traT, tsh) were especially frequent among the isolates (from 66.6% to 89.6%). According to the Clermont method of ECOR phylogenetic typing, isolates belonged to group D (47.2%), to group A (27.8%), to group B2 (17.4%) and to group B1 (7.6%); the group B2 isolates contained the highest number of virulence-associated genes. Clonal relationship analysis using the ARDRA method revealed a similarity level of 57% or higher among isolates, but no endemic clone. The virulence of the isolates was confirmed in vivo in one-day-old chicks. Most isolates (72.9%) killed all infected chicks within 7 days, and 65 isolates (38.1%) killed most of them within 24 hours. In order to analyze differences in virulence among the APEC isolates, we created a pathogenicity score by combining the times of death with the clinical symptoms noted. By looking for significant associations between the presence of virulence-associated genes and the pathogenicity score, we found that the presence of genes for invasins ibeA and gimB and for group II capsule KpsMTII increased virulence, while the presence of pic decreased virulence. The fact that ibeA, gimB and KpsMTII are characteristic of neonatal meningitis E. coli (NMEC) suggests that genes of NMEC in APEC increase virulence of strains.application/pdfengPloS one. San Francisco. Vol. 8, no. 8 (Aug. 2013), e72322, 9 p.Estatística médicaGenotypes and pathogenicity of cellulitis isolates reveal traits that modulate APEC virulenceEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000897978.pdf.txt000897978.pdf.txtExtracted Texttext/plain49404http://www.lume.ufrgs.br/bitstream/10183/115264/2/000897978.pdf.txt8c0f811055216dbe587d523acfeccf65MD52ORIGINAL000897978.pdf000897978.pdfTexto completo (inglês)application/pdf631764http://www.lume.ufrgs.br/bitstream/10183/115264/1/000897978.pdfd922353420237cedcb221bc55aca54efMD51THUMBNAIL000897978.pdf.jpg000897978.pdf.jpgGenerated Thumbnailimage/jpeg2130http://www.lume.ufrgs.br/bitstream/10183/115264/3/000897978.pdf.jpg7418241dd5cbbd219699c3e18c250164MD5310183/1152642023-09-23 03:36:09.962223oai:www.lume.ufrgs.br:10183/115264Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-09-23T06:36:09Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Genotypes and pathogenicity of cellulitis isolates reveal traits that modulate APEC virulence |
title |
Genotypes and pathogenicity of cellulitis isolates reveal traits that modulate APEC virulence |
spellingShingle |
Genotypes and pathogenicity of cellulitis isolates reveal traits that modulate APEC virulence Barbieri, Nicolle Lima Estatística médica |
title_short |
Genotypes and pathogenicity of cellulitis isolates reveal traits that modulate APEC virulence |
title_full |
Genotypes and pathogenicity of cellulitis isolates reveal traits that modulate APEC virulence |
title_fullStr |
Genotypes and pathogenicity of cellulitis isolates reveal traits that modulate APEC virulence |
title_full_unstemmed |
Genotypes and pathogenicity of cellulitis isolates reveal traits that modulate APEC virulence |
title_sort |
Genotypes and pathogenicity of cellulitis isolates reveal traits that modulate APEC virulence |
author |
Barbieri, Nicolle Lima |
author_facet |
Barbieri, Nicolle Lima Oliveira, Aline Luísa de Tejkowski, Thiago Moreira Pavanelo, Daniel Brisotto Rocha, Débora Assumpção Matter, Leticia Beatriz Callegari-Jacques, Sidia Maria Brito, Benito Guimarães de Horn, Fabiana |
author_role |
author |
author2 |
Oliveira, Aline Luísa de Tejkowski, Thiago Moreira Pavanelo, Daniel Brisotto Rocha, Débora Assumpção Matter, Leticia Beatriz Callegari-Jacques, Sidia Maria Brito, Benito Guimarães de Horn, Fabiana |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Barbieri, Nicolle Lima Oliveira, Aline Luísa de Tejkowski, Thiago Moreira Pavanelo, Daniel Brisotto Rocha, Débora Assumpção Matter, Leticia Beatriz Callegari-Jacques, Sidia Maria Brito, Benito Guimarães de Horn, Fabiana |
dc.subject.por.fl_str_mv |
Estatística médica |
topic |
Estatística médica |
description |
We characterized 144 Escherichia coli isolates from severe cellulitis lesions in broiler chickens from South Brazil. Analysis of susceptibility to 15 antimicrobials revealed frequencies of resistance of less than 30% for most antimicrobials except tetracycline (70%) and sulphonamides (60%). The genotyping of 34 virulence-associated genes revealed that all the isolates harbored virulence factors related to adhesion, iron acquisition and serum resistance, which are characteristic of the avian pathogenic E. coli (APEC) pathotype. ColV plasmid-associated genes (cvi/cva, iroN, iss, iucD, sitD, traT, tsh) were especially frequent among the isolates (from 66.6% to 89.6%). According to the Clermont method of ECOR phylogenetic typing, isolates belonged to group D (47.2%), to group A (27.8%), to group B2 (17.4%) and to group B1 (7.6%); the group B2 isolates contained the highest number of virulence-associated genes. Clonal relationship analysis using the ARDRA method revealed a similarity level of 57% or higher among isolates, but no endemic clone. The virulence of the isolates was confirmed in vivo in one-day-old chicks. Most isolates (72.9%) killed all infected chicks within 7 days, and 65 isolates (38.1%) killed most of them within 24 hours. In order to analyze differences in virulence among the APEC isolates, we created a pathogenicity score by combining the times of death with the clinical symptoms noted. By looking for significant associations between the presence of virulence-associated genes and the pathogenicity score, we found that the presence of genes for invasins ibeA and gimB and for group II capsule KpsMTII increased virulence, while the presence of pic decreased virulence. The fact that ibeA, gimB and KpsMTII are characteristic of neonatal meningitis E. coli (NMEC) suggests that genes of NMEC in APEC increase virulence of strains. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
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2015-04-14T01:57:40Z |
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1932-6203 |
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PloS one. San Francisco. Vol. 8, no. 8 (Aug. 2013), e72322, 9 p. |
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