Apolipoprotein CIII polymorphism and triglyceride levels of a japanese population living in southern Brazil

Detalhes bibliográficos
Autor(a) principal: Parzianello, Leandro
Data de Publicação: 2008
Outros Autores: Volpe, Graziela de Oliveira, Coelho, Janice Carneiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/21228
Resumo: Apolipoprotein CIII (apo-CIII) participates in the regulation of triglyceride-rich lipoprotein metabolism. Several polymorphic sites have been detected within and around the apo-CIII gene. Here, we examined the relationship between apo-CIII Sst I polymorphism (CC, CG, GG genotypes) and plasma triglyceride (TG) levels in a group of 159 Japanese individuals living in Southern Brazil. The sample was divided into a group of Japanese descendants (N = 51) with high TG (HTG; >200 mg/dL) and a group of Japanese descendants (N = 108) with normal TG (NTG; <200 mg/dL). TG and total cholesterol levels were analyzed by an enzymatic method using the Labtest-Diagnostic kit and high- and low-density lipoproteins by a direct method using the Labtest-Diagnostic kit and DiaSys Diagnostic System International kit, respectively. A 428-bp sequence of apo-CIII gene was amplified using oligonucleotide primers 5' GGT GAC CGA TGG CTT CAG TTC CCT GA 3' and 5' CAG AAG GTG GAT AGA GCG CTG GCC T 3'. The PCR products were digested with a restriction endonuclease Sst I. Rare G allele was highly prevalent in our study population (0.416) compared to Caucasians (0.00-0.11). G allele was almost two times more prevalent in the HTG group compared to the NTG group (P < 0.001). The genotype distribution was consistent with the Hardy-Weinberg equilibrium. There was a significant association between rare G allele and HTG in Japanese individuals living in Southern Brazil as indicated by one-way ANOVA, P < 0.05.
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spelling Parzianello, LeandroVolpe, Graziela de OliveiraCoelho, Janice Carneiro2010-04-24T04:15:52Z20080100-879Xhttp://hdl.handle.net/10183/21228000695594Apolipoprotein CIII (apo-CIII) participates in the regulation of triglyceride-rich lipoprotein metabolism. Several polymorphic sites have been detected within and around the apo-CIII gene. Here, we examined the relationship between apo-CIII Sst I polymorphism (CC, CG, GG genotypes) and plasma triglyceride (TG) levels in a group of 159 Japanese individuals living in Southern Brazil. The sample was divided into a group of Japanese descendants (N = 51) with high TG (HTG; >200 mg/dL) and a group of Japanese descendants (N = 108) with normal TG (NTG; <200 mg/dL). TG and total cholesterol levels were analyzed by an enzymatic method using the Labtest-Diagnostic kit and high- and low-density lipoproteins by a direct method using the Labtest-Diagnostic kit and DiaSys Diagnostic System International kit, respectively. A 428-bp sequence of apo-CIII gene was amplified using oligonucleotide primers 5' GGT GAC CGA TGG CTT CAG TTC CCT GA 3' and 5' CAG AAG GTG GAT AGA GCG CTG GCC T 3'. The PCR products were digested with a restriction endonuclease Sst I. Rare G allele was highly prevalent in our study population (0.416) compared to Caucasians (0.00-0.11). G allele was almost two times more prevalent in the HTG group compared to the NTG group (P < 0.001). The genotype distribution was consistent with the Hardy-Weinberg equilibrium. There was a significant association between rare G allele and HTG in Japanese individuals living in Southern Brazil as indicated by one-way ANOVA, P < 0.05.application/pdfengBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 41, n.6 (June 2008), p. 462-467Apolipoproteína C-IIIPolimorfismoTriglicerídeosTriglyceridesSst I polymorphismApolipoprotein CIIIJapanese populationApolipoprotein CIII polymorphism and triglyceride levels of a japanese population living in southern Brazilinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000695594.pdf000695594.pdfTexto completo (inglês)application/pdf62894http://www.lume.ufrgs.br/bitstream/10183/21228/1/000695594.pdf90f0e5f29bcbfa0f2d8b93ba42bc991aMD51TEXT000695594.pdf.txt000695594.pdf.txtExtracted Texttext/plain26468http://www.lume.ufrgs.br/bitstream/10183/21228/2/000695594.pdf.txtff0adf5d41614be4134e3558fb894f53MD52THUMBNAIL000695594.pdf.jpg000695594.pdf.jpgGenerated Thumbnailimage/jpeg2150http://www.lume.ufrgs.br/bitstream/10183/21228/3/000695594.pdf.jpg8ecb83b254b039c451f179988b328b71MD5310183/212282021-11-20 05:44:08.742711oai:www.lume.ufrgs.br:10183/21228Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-11-20T07:44:08Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Apolipoprotein CIII polymorphism and triglyceride levels of a japanese population living in southern Brazil
title Apolipoprotein CIII polymorphism and triglyceride levels of a japanese population living in southern Brazil
spellingShingle Apolipoprotein CIII polymorphism and triglyceride levels of a japanese population living in southern Brazil
Parzianello, Leandro
Apolipoproteína C-III
Polimorfismo
Triglicerídeos
Triglycerides
Sst I polymorphism
Apolipoprotein CIII
Japanese population
title_short Apolipoprotein CIII polymorphism and triglyceride levels of a japanese population living in southern Brazil
title_full Apolipoprotein CIII polymorphism and triglyceride levels of a japanese population living in southern Brazil
title_fullStr Apolipoprotein CIII polymorphism and triglyceride levels of a japanese population living in southern Brazil
title_full_unstemmed Apolipoprotein CIII polymorphism and triglyceride levels of a japanese population living in southern Brazil
title_sort Apolipoprotein CIII polymorphism and triglyceride levels of a japanese population living in southern Brazil
author Parzianello, Leandro
author_facet Parzianello, Leandro
Volpe, Graziela de Oliveira
Coelho, Janice Carneiro
author_role author
author2 Volpe, Graziela de Oliveira
Coelho, Janice Carneiro
author2_role author
author
dc.contributor.author.fl_str_mv Parzianello, Leandro
Volpe, Graziela de Oliveira
Coelho, Janice Carneiro
dc.subject.por.fl_str_mv Apolipoproteína C-III
Polimorfismo
Triglicerídeos
topic Apolipoproteína C-III
Polimorfismo
Triglicerídeos
Triglycerides
Sst I polymorphism
Apolipoprotein CIII
Japanese population
dc.subject.eng.fl_str_mv Triglycerides
Sst I polymorphism
Apolipoprotein CIII
Japanese population
description Apolipoprotein CIII (apo-CIII) participates in the regulation of triglyceride-rich lipoprotein metabolism. Several polymorphic sites have been detected within and around the apo-CIII gene. Here, we examined the relationship between apo-CIII Sst I polymorphism (CC, CG, GG genotypes) and plasma triglyceride (TG) levels in a group of 159 Japanese individuals living in Southern Brazil. The sample was divided into a group of Japanese descendants (N = 51) with high TG (HTG; >200 mg/dL) and a group of Japanese descendants (N = 108) with normal TG (NTG; <200 mg/dL). TG and total cholesterol levels were analyzed by an enzymatic method using the Labtest-Diagnostic kit and high- and low-density lipoproteins by a direct method using the Labtest-Diagnostic kit and DiaSys Diagnostic System International kit, respectively. A 428-bp sequence of apo-CIII gene was amplified using oligonucleotide primers 5' GGT GAC CGA TGG CTT CAG TTC CCT GA 3' and 5' CAG AAG GTG GAT AGA GCG CTG GCC T 3'. The PCR products were digested with a restriction endonuclease Sst I. Rare G allele was highly prevalent in our study population (0.416) compared to Caucasians (0.00-0.11). G allele was almost two times more prevalent in the HTG group compared to the NTG group (P < 0.001). The genotype distribution was consistent with the Hardy-Weinberg equilibrium. There was a significant association between rare G allele and HTG in Japanese individuals living in Southern Brazil as indicated by one-way ANOVA, P < 0.05.
publishDate 2008
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dc.relation.ispartof.pt_BR.fl_str_mv Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 41, n.6 (June 2008), p. 462-467
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