N-acetylcysteine downregulates phosphorylated p-38 expression but does not reverse the increased superoxide anion levels in the spinal cord of rats with neuropathic pain
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/225947 |
Resumo: | We determined the effect of N-acetylcysteine (NAC) on the expression of the phosphorylated p38 (p-p38) protein and superoxide anion generation (SAG), two important players in the processing of neuropathic pain, in the lumbosacral spinal cord of rats with chronic constriction injury (CCI)-induced neuropathic pain. The sciatic functional index (SFI) was also measured to assess the functional recovery post-nerve lesion. Thirty-six male Wistar rats were divided equally into the following groups: Naive (rats did not undergo surgical manipulation); Sham (rats in which all surgical procedures involved in CCI were used except the ligature), and CCI (rats in which four ligatures were tied loosely around the right common sciatic nerve), which received 2, 4, or 8 intraperitoneal injections of NAC (150 mg kg–1 day–1 ) or saline beginning 4 h after CCI. Rats were sacrificed 1, 3, and 7 days after CCI. The SFI was measured on these days and the lumbosacral spinal cord was used for analysis of p-p38 expression and SAG. CCI induced a decrease in SFI as well as an increase in p-p38 expression and SAG in the spinal cord. The SFI showed a partial recovery at day 7 in saline-treated CCI rats, but recovery was improved in NAC-treated CCI rats. NAC induced a downregulation in p-p38 expression at all time-points evaluated, but did not reverse the increased SAG induced by CCI. Since p-p38 is a mediator in neuropathic pain and/or nerve regeneration, modulation of this protein may play a role in NAC-induced effects in CCI rats. |
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Horst, AndréaSouza, Jéssica Araújo deSantos, Maria do Carmo QuevedoRiffel, Ana Paula KonzenKolberg, CarolinaRibeiro, Maria Flavia MarquesFraga, Luciano Sturmer dePartata, Wania Aparecida2021-08-20T04:10:05Z20170100-879Xhttp://hdl.handle.net/10183/225947001024399We determined the effect of N-acetylcysteine (NAC) on the expression of the phosphorylated p38 (p-p38) protein and superoxide anion generation (SAG), two important players in the processing of neuropathic pain, in the lumbosacral spinal cord of rats with chronic constriction injury (CCI)-induced neuropathic pain. The sciatic functional index (SFI) was also measured to assess the functional recovery post-nerve lesion. Thirty-six male Wistar rats were divided equally into the following groups: Naive (rats did not undergo surgical manipulation); Sham (rats in which all surgical procedures involved in CCI were used except the ligature), and CCI (rats in which four ligatures were tied loosely around the right common sciatic nerve), which received 2, 4, or 8 intraperitoneal injections of NAC (150 mg kg–1 day–1 ) or saline beginning 4 h after CCI. Rats were sacrificed 1, 3, and 7 days after CCI. The SFI was measured on these days and the lumbosacral spinal cord was used for analysis of p-p38 expression and SAG. CCI induced a decrease in SFI as well as an increase in p-p38 expression and SAG in the spinal cord. The SFI showed a partial recovery at day 7 in saline-treated CCI rats, but recovery was improved in NAC-treated CCI rats. NAC induced a downregulation in p-p38 expression at all time-points evaluated, but did not reverse the increased SAG induced by CCI. Since p-p38 is a mediator in neuropathic pain and/or nerve regeneration, modulation of this protein may play a role in NAC-induced effects in CCI rats.application/pdfengBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 50, no. 2 (2017), e5801, 6 p.AcetilcisteínaNeuralgiaNociceptividadeNervo isquiáticoSciatic functional indexChronic constrictionSciatic nerveMitogen-activated protein kinaseAntinociceptionMechanical thresholdN-acetylcysteine downregulates phosphorylated p-38 expression but does not reverse the increased superoxide anion levels in the spinal cord of rats with neuropathic paininfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001024399.pdf.txt001024399.pdf.txtExtracted Texttext/plain25306http://www.lume.ufrgs.br/bitstream/10183/225947/2/001024399.pdf.txtddd49ab47438e109162c359c52ddd1f8MD52ORIGINAL001024399.pdfTexto completo (inglês)application/pdf1006366http://www.lume.ufrgs.br/bitstream/10183/225947/1/001024399.pdfde9c44cf388e66fe90a303194ef6d702MD5110183/2259472021-11-20 05:55:46.717779oai:www.lume.ufrgs.br:10183/225947Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-11-20T07:55:46Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
N-acetylcysteine downregulates phosphorylated p-38 expression but does not reverse the increased superoxide anion levels in the spinal cord of rats with neuropathic pain |
title |
N-acetylcysteine downregulates phosphorylated p-38 expression but does not reverse the increased superoxide anion levels in the spinal cord of rats with neuropathic pain |
spellingShingle |
N-acetylcysteine downregulates phosphorylated p-38 expression but does not reverse the increased superoxide anion levels in the spinal cord of rats with neuropathic pain Horst, Andréa Acetilcisteína Neuralgia Nociceptividade Nervo isquiático Sciatic functional index Chronic constriction Sciatic nerve Mitogen-activated protein kinase Antinociception Mechanical threshold |
title_short |
N-acetylcysteine downregulates phosphorylated p-38 expression but does not reverse the increased superoxide anion levels in the spinal cord of rats with neuropathic pain |
title_full |
N-acetylcysteine downregulates phosphorylated p-38 expression but does not reverse the increased superoxide anion levels in the spinal cord of rats with neuropathic pain |
title_fullStr |
N-acetylcysteine downregulates phosphorylated p-38 expression but does not reverse the increased superoxide anion levels in the spinal cord of rats with neuropathic pain |
title_full_unstemmed |
N-acetylcysteine downregulates phosphorylated p-38 expression but does not reverse the increased superoxide anion levels in the spinal cord of rats with neuropathic pain |
title_sort |
N-acetylcysteine downregulates phosphorylated p-38 expression but does not reverse the increased superoxide anion levels in the spinal cord of rats with neuropathic pain |
author |
Horst, Andréa |
author_facet |
Horst, Andréa Souza, Jéssica Araújo de Santos, Maria do Carmo Quevedo Riffel, Ana Paula Konzen Kolberg, Carolina Ribeiro, Maria Flavia Marques Fraga, Luciano Sturmer de Partata, Wania Aparecida |
author_role |
author |
author2 |
Souza, Jéssica Araújo de Santos, Maria do Carmo Quevedo Riffel, Ana Paula Konzen Kolberg, Carolina Ribeiro, Maria Flavia Marques Fraga, Luciano Sturmer de Partata, Wania Aparecida |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Horst, Andréa Souza, Jéssica Araújo de Santos, Maria do Carmo Quevedo Riffel, Ana Paula Konzen Kolberg, Carolina Ribeiro, Maria Flavia Marques Fraga, Luciano Sturmer de Partata, Wania Aparecida |
dc.subject.por.fl_str_mv |
Acetilcisteína Neuralgia Nociceptividade Nervo isquiático |
topic |
Acetilcisteína Neuralgia Nociceptividade Nervo isquiático Sciatic functional index Chronic constriction Sciatic nerve Mitogen-activated protein kinase Antinociception Mechanical threshold |
dc.subject.eng.fl_str_mv |
Sciatic functional index Chronic constriction Sciatic nerve Mitogen-activated protein kinase Antinociception Mechanical threshold |
description |
We determined the effect of N-acetylcysteine (NAC) on the expression of the phosphorylated p38 (p-p38) protein and superoxide anion generation (SAG), two important players in the processing of neuropathic pain, in the lumbosacral spinal cord of rats with chronic constriction injury (CCI)-induced neuropathic pain. The sciatic functional index (SFI) was also measured to assess the functional recovery post-nerve lesion. Thirty-six male Wistar rats were divided equally into the following groups: Naive (rats did not undergo surgical manipulation); Sham (rats in which all surgical procedures involved in CCI were used except the ligature), and CCI (rats in which four ligatures were tied loosely around the right common sciatic nerve), which received 2, 4, or 8 intraperitoneal injections of NAC (150 mg kg–1 day–1 ) or saline beginning 4 h after CCI. Rats were sacrificed 1, 3, and 7 days after CCI. The SFI was measured on these days and the lumbosacral spinal cord was used for analysis of p-p38 expression and SAG. CCI induced a decrease in SFI as well as an increase in p-p38 expression and SAG in the spinal cord. The SFI showed a partial recovery at day 7 in saline-treated CCI rats, but recovery was improved in NAC-treated CCI rats. NAC induced a downregulation in p-p38 expression at all time-points evaluated, but did not reverse the increased SAG induced by CCI. Since p-p38 is a mediator in neuropathic pain and/or nerve regeneration, modulation of this protein may play a role in NAC-induced effects in CCI rats. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2021-08-20T04:10:05Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/225947 |
dc.identifier.issn.pt_BR.fl_str_mv |
0100-879X |
dc.identifier.nrb.pt_BR.fl_str_mv |
001024399 |
identifier_str_mv |
0100-879X 001024399 |
url |
http://hdl.handle.net/10183/225947 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 50, no. 2 (2017), e5801, 6 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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