New insights of the local immune response against both fertile and infertile hydatid cysts

Detalhes bibliográficos
Autor(a) principal: Hidalgo, Christian
Data de Publicação: 2019
Outros Autores: Stoore, Caroll, Strull, Karen, Franco, Carmen, Corrêa, Felipe, Jiménez, Maurício, Hernández, Marcela, Lorenzatto, Karina Rodrigues, Ferreira, Henrique Bunselmeyer, Alvarez Rojas, Christian, Galanti, Norbel, Paredes, Rodolfo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/221532
Resumo: Background Cystic echinococcosis is caused by the metacestode of the zoonotic flatworm Echinococcus granulosus. Within the viscera of the intermediate host, the metacestode grows as a unilocular cyst known as hydatid cyst. This cyst is comprised of two layers of parasite origin: germinal and laminated layers, and one of host origin: the adventitial layer, that encapsulates the parasite. This adventitial layer is composed of collagen fibers, epithelioid cells, eosinophils and lymphocytes. To establish itself inside the host, the germinal layer produces the laminated layer, and to continue its life cycle, generates protoscoleces. Some cysts are unable to produce protoscoleces, and are defined as infertile cysts. The molecular mechanisms involved in cyst fertility are not clear, however, the host immune response could play a crucial role. Methodology/Principal findings We collected hydatid cysts from both liver and lungs of slaughtered cattle, and histological sections of fertile, infertile and small hydatid cysts were stained with haematoxylin-eosin. A common feature observed in infertile cysts was the disorganization of the laminated layer by the infiltration of host immune cells. These infiltrating cells eventually destroy parts of laminated layer. Immunohistochemical analysis of both parasite and host antigens, identify these cells as cattle macrophages and are present inside the cysts associated to germinal layer. Conclusions/Significance This is the first report that indicates to cell from immune system present in adventitial layer of infertile bovine hydatid cysts could disrupt the laminated layer, infiltrating and probably causing the infertility of cyst.
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spelling Hidalgo, ChristianStoore, CarollStrull, KarenFranco, CarmenCorrêa, FelipeJiménez, MaurícioHernández, MarcelaLorenzatto, Karina RodriguesFerreira, Henrique BunselmeyerAlvarez Rojas, ChristianGalanti, NorbelParedes, Rodolfo2021-05-26T04:40:51Z20191932-6203http://hdl.handle.net/10183/221532001105663Background Cystic echinococcosis is caused by the metacestode of the zoonotic flatworm Echinococcus granulosus. Within the viscera of the intermediate host, the metacestode grows as a unilocular cyst known as hydatid cyst. This cyst is comprised of two layers of parasite origin: germinal and laminated layers, and one of host origin: the adventitial layer, that encapsulates the parasite. This adventitial layer is composed of collagen fibers, epithelioid cells, eosinophils and lymphocytes. To establish itself inside the host, the germinal layer produces the laminated layer, and to continue its life cycle, generates protoscoleces. Some cysts are unable to produce protoscoleces, and are defined as infertile cysts. The molecular mechanisms involved in cyst fertility are not clear, however, the host immune response could play a crucial role. Methodology/Principal findings We collected hydatid cysts from both liver and lungs of slaughtered cattle, and histological sections of fertile, infertile and small hydatid cysts were stained with haematoxylin-eosin. A common feature observed in infertile cysts was the disorganization of the laminated layer by the infiltration of host immune cells. These infiltrating cells eventually destroy parts of laminated layer. Immunohistochemical analysis of both parasite and host antigens, identify these cells as cattle macrophages and are present inside the cysts associated to germinal layer. Conclusions/Significance This is the first report that indicates to cell from immune system present in adventitial layer of infertile bovine hydatid cysts could disrupt the laminated layer, infiltrating and probably causing the infertility of cyst.application/pdfengPLoS ONE. San Francisco. Vol. 14, no. 1 (Jan. 2019), e0211542, 10 p.Echinococcus granulosusEquinococoseNew insights of the local immune response against both fertile and infertile hydatid cystsEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001105663.pdf.txt001105663.pdf.txtExtracted Texttext/plain47150http://www.lume.ufrgs.br/bitstream/10183/221532/2/001105663.pdf.txt69c6a3b66a4182bdf7553f31acf16a33MD52ORIGINAL001105663.pdfTexto completo (inglês)application/pdf3952168http://www.lume.ufrgs.br/bitstream/10183/221532/1/001105663.pdf7cf4870008a19a38aec518ad64614395MD5110183/2215322023-09-24 03:39:15.256411oai:www.lume.ufrgs.br:10183/221532Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-09-24T06:39:15Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv New insights of the local immune response against both fertile and infertile hydatid cysts
title New insights of the local immune response against both fertile and infertile hydatid cysts
spellingShingle New insights of the local immune response against both fertile and infertile hydatid cysts
Hidalgo, Christian
Echinococcus granulosus
Equinococose
title_short New insights of the local immune response against both fertile and infertile hydatid cysts
title_full New insights of the local immune response against both fertile and infertile hydatid cysts
title_fullStr New insights of the local immune response against both fertile and infertile hydatid cysts
title_full_unstemmed New insights of the local immune response against both fertile and infertile hydatid cysts
title_sort New insights of the local immune response against both fertile and infertile hydatid cysts
author Hidalgo, Christian
author_facet Hidalgo, Christian
Stoore, Caroll
Strull, Karen
Franco, Carmen
Corrêa, Felipe
Jiménez, Maurício
Hernández, Marcela
Lorenzatto, Karina Rodrigues
Ferreira, Henrique Bunselmeyer
Alvarez Rojas, Christian
Galanti, Norbel
Paredes, Rodolfo
author_role author
author2 Stoore, Caroll
Strull, Karen
Franco, Carmen
Corrêa, Felipe
Jiménez, Maurício
Hernández, Marcela
Lorenzatto, Karina Rodrigues
Ferreira, Henrique Bunselmeyer
Alvarez Rojas, Christian
Galanti, Norbel
Paredes, Rodolfo
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Hidalgo, Christian
Stoore, Caroll
Strull, Karen
Franco, Carmen
Corrêa, Felipe
Jiménez, Maurício
Hernández, Marcela
Lorenzatto, Karina Rodrigues
Ferreira, Henrique Bunselmeyer
Alvarez Rojas, Christian
Galanti, Norbel
Paredes, Rodolfo
dc.subject.por.fl_str_mv Echinococcus granulosus
Equinococose
topic Echinococcus granulosus
Equinococose
description Background Cystic echinococcosis is caused by the metacestode of the zoonotic flatworm Echinococcus granulosus. Within the viscera of the intermediate host, the metacestode grows as a unilocular cyst known as hydatid cyst. This cyst is comprised of two layers of parasite origin: germinal and laminated layers, and one of host origin: the adventitial layer, that encapsulates the parasite. This adventitial layer is composed of collagen fibers, epithelioid cells, eosinophils and lymphocytes. To establish itself inside the host, the germinal layer produces the laminated layer, and to continue its life cycle, generates protoscoleces. Some cysts are unable to produce protoscoleces, and are defined as infertile cysts. The molecular mechanisms involved in cyst fertility are not clear, however, the host immune response could play a crucial role. Methodology/Principal findings We collected hydatid cysts from both liver and lungs of slaughtered cattle, and histological sections of fertile, infertile and small hydatid cysts were stained with haematoxylin-eosin. A common feature observed in infertile cysts was the disorganization of the laminated layer by the infiltration of host immune cells. These infiltrating cells eventually destroy parts of laminated layer. Immunohistochemical analysis of both parasite and host antigens, identify these cells as cattle macrophages and are present inside the cysts associated to germinal layer. Conclusions/Significance This is the first report that indicates to cell from immune system present in adventitial layer of infertile bovine hydatid cysts could disrupt the laminated layer, infiltrating and probably causing the infertility of cyst.
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2021-05-26T04:40:51Z
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dc.relation.ispartof.pt_BR.fl_str_mv PLoS ONE. San Francisco. Vol. 14, no. 1 (Jan. 2019), e0211542, 10 p.
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