Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets

Detalhes bibliográficos
Autor(a) principal: Morais, Guilherme Loss de
Data de Publicação: 2013
Outros Autores: Waterhouse, Peter M., Margis, Rogerio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/110062
Resumo: tRNA-derived RNA fragments (tRFs) are 19mer small RNAs that associate with Argonaute (AGO) proteins in humans. However, in plants, it is unknown if tRFs bind with AGO proteins. Here, using public deep sequencing libraries of immunoprecipitated Argonaute proteins (AGO-IP) and bioinformatics approaches, we identified the Arabidopsis thaliana AGO-IP tRFs. Moreover, using three degradome deep sequencing libraries, we identified four putative tRF targets. The expression pattern of tRFs, based on deep sequencing data, was also analyzed under abiotic and biotic stresses. The results obtained here represent a useful starting point for future studies on tRFs in plants.
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spelling Morais, Guilherme Loss deWaterhouse, Peter M.Margis, Rogerio2015-02-12T02:15:46Z20131745-6150http://hdl.handle.net/10183/110062000893284tRNA-derived RNA fragments (tRFs) are 19mer small RNAs that associate with Argonaute (AGO) proteins in humans. However, in plants, it is unknown if tRFs bind with AGO proteins. Here, using public deep sequencing libraries of immunoprecipitated Argonaute proteins (AGO-IP) and bioinformatics approaches, we identified the Arabidopsis thaliana AGO-IP tRFs. Moreover, using three degradome deep sequencing libraries, we identified four putative tRF targets. The expression pattern of tRFs, based on deep sequencing data, was also analyzed under abiotic and biotic stresses. The results obtained here represent a useful starting point for future studies on tRFs in plants.application/pdfengBiology direct. London. Vol. 8, no. 6 (17 May 2013), 5 p.Arabidopsis thalianatRNAsSmall RNAtRFstRNA-derived RNA fragmentsArgonaute and ArabidopsisDescription of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targetsEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000893284.pdf000893284.pdfTexto completo (inglês)application/pdf983753http://www.lume.ufrgs.br/bitstream/10183/110062/1/000893284.pdf35a7e23a2bb1a44414117a5c103d7840MD51TEXT000893284.pdf.txt000893284.pdf.txtExtracted Texttext/plain19094http://www.lume.ufrgs.br/bitstream/10183/110062/2/000893284.pdf.txtecab38205c45109c092220da0fbd7489MD52THUMBNAIL000893284.pdf.jpg000893284.pdf.jpgGenerated Thumbnailimage/jpeg2059http://www.lume.ufrgs.br/bitstream/10183/110062/3/000893284.pdf.jpg916352f0d594caa0cd1c718841cebe94MD5310183/1100622018-10-23 09:17:34.592oai:www.lume.ufrgs.br:10183/110062Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-23T12:17:34Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
title Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
spellingShingle Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
Morais, Guilherme Loss de
Arabidopsis thaliana
tRNAs
Small RNA
tRFs
tRNA-derived RNA fragments
Argonaute and Arabidopsis
title_short Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
title_full Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
title_fullStr Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
title_full_unstemmed Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
title_sort Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets
author Morais, Guilherme Loss de
author_facet Morais, Guilherme Loss de
Waterhouse, Peter M.
Margis, Rogerio
author_role author
author2 Waterhouse, Peter M.
Margis, Rogerio
author2_role author
author
dc.contributor.author.fl_str_mv Morais, Guilherme Loss de
Waterhouse, Peter M.
Margis, Rogerio
dc.subject.por.fl_str_mv Arabidopsis thaliana
topic Arabidopsis thaliana
tRNAs
Small RNA
tRFs
tRNA-derived RNA fragments
Argonaute and Arabidopsis
dc.subject.eng.fl_str_mv tRNAs
Small RNA
tRFs
tRNA-derived RNA fragments
Argonaute and Arabidopsis
description tRNA-derived RNA fragments (tRFs) are 19mer small RNAs that associate with Argonaute (AGO) proteins in humans. However, in plants, it is unknown if tRFs bind with AGO proteins. Here, using public deep sequencing libraries of immunoprecipitated Argonaute proteins (AGO-IP) and bioinformatics approaches, we identified the Arabidopsis thaliana AGO-IP tRFs. Moreover, using three degradome deep sequencing libraries, we identified four putative tRF targets. The expression pattern of tRFs, based on deep sequencing data, was also analyzed under abiotic and biotic stresses. The results obtained here represent a useful starting point for future studies on tRFs in plants.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2015-02-12T02:15:46Z
dc.type.driver.fl_str_mv Estrangeiro
info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/110062
dc.identifier.issn.pt_BR.fl_str_mv 1745-6150
dc.identifier.nrb.pt_BR.fl_str_mv 000893284
identifier_str_mv 1745-6150
000893284
url http://hdl.handle.net/10183/110062
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Biology direct. London. Vol. 8, no. 6 (17 May 2013), 5 p.
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