Imunobloqueio de RAGE reduz a sinalização de NFkB e aumenta a atividade de catalase na retina após Uveíte induzida por Endotoxina

• Autor(a) principal: Gomes, Henrique Mautone
• Data de Publicação: 2019
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/237552
• topic: Endotoxin Induced Uveitis; Anti-RAGE IgG; Retinal tissue; Catalase activity; NFKB signaling

The receptor for advanced glycation end products (RAGE) is a multiligand membrane receptor associated with a variety of roles depending on the tissue evaluated and its activation is believed to sustain a proinflammatory state in the system. Also, it has been shown that blockage of RAGE can somehow display a protective effect against insults induced by Lipopolysaccharide (LPS) endotoxemia. LPS is widely used to mimic strong and acute inflammation in mammals and also as a model for Endotoxin Induced Uveitis (EIU) in rodents. Materials and Methods: In this work we evaluated if pre-treatment with anti-RAGE IgG could have a protective effect in the retinas of Wistar Rats. Animals (60 days old) were randomly distributed into 2 treated groups and 2 control groups and had their retinas collected 24 hours after induction of EIU via LPS endotoxemia. Catalase activity and also the levels of protein and lipid oxidation were used as oxidative stress markers and some RAGE downstream signaling molecules were quantified by western blotting. Results: We observed that RAGE blockage associated with LPS reduced the phosphorylation of ERK1/2, Stat3 and P65 and increased Catalase activity while not altering oxidative damage markers. Conclusion: Our results indicate us that RAGE has a pivotal role in retinal tissue mainly mediating signal transducing factors. Oxidative damage markers were not elevated in retinal tissue however this may be a time-dependent issue since we used a short-term protocol for evaluating LPS endotoxemia and phosphorylation of NFKB ligands was observed.