Peripheral oxidative stress biomarkers in spinocerebellar ataxia type 3/Machado–Joseph disease
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/182527 |
Resumo: | Objectives: Spinocerebellar ataxia type 3/Machado–Joseph disease (SCA3/MJD) is a polyglutamine disorder with no current disease-modifying treatment. Conformational changes in mutant ataxin-3 trigger different pathogenic cascades, including reactive oxygen species (ROS) generation; however, the clinical relevance of oxidative stress elements as peripheral biomarkers of SCA3/MJD remains unknown. We aimed to evaluate ROS production and antioxidant defense capacity in symptomatic and presymptomatic SCA3/MJD individuals and correlate these markers with clinical and molecular data with the goal of assessing their properties as disease biomarkers. Methods: Molecularly confirmed SCA3/MJD carriers and controls were included in an exploratory case–control study. Serum ROS, measured by 2′,7′-dichlorofluorescein diacetate (DCFH-DA) as well as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) antioxidant enzyme activities, levels were assessed. Results: Fifty-eight early/moderate stage symptomatic SCA3/MJD, 12 presymptomatic SCA3/MJD, and 47 control individuals were assessed. The DCFH-DA levels in the symptomatic group were 152.82 nmol/mg of protein [95% confidence interval (CI), 82.57–223.08, p < 0.001] higher than in the control and 243.80 nmol/mg of protein (95% CI, 130.64–356.96, p < 0.001) higher than in the presymptomatic group. The SOD activity in the symptomatic group was 3 U/mg of protein (95% CI, 0.015–6.00, p = 0.048) lower than in the presymptomatic group. The GSH-Px activity in the symptomatic group was 13.96 U/mg of protein (95% CI, 5.90–22.03, p < 0.001) lower than in the control group and 20.52 U/mg of protein (95% CI, 6.79–34.24, p < 0.001) lower than in the presymptomatic group and was inversely correlated with the neurological examination score for spinocerebellar ataxias (R = −0.309, p = 0.049). Conclusion: Early/moderate stage SCA3/MJD patients presented a decreased antioxidant capacity and increased ROS generation. GSH-Px activity was the most promising oxidative stress disease biomarker in SCA3/MJD. Further longitudinal studies are necessary to identify both the roles of redox parameters in SCA3/MJD pathophysiology and as surrogate outcomes for clinical trials. |
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Assis, Adriano Martimbianco deSaute, Jonas Alex MoralesSantos, Aline Longoni dosHaas, Clarissa BrancoTorrez, Vitor RoccoBrochier, Andressa WignerSouza, Gabriele NunesFurtado, Gabriel VasataGheno, Tailise ConteRusso, AlineMonte, Thais LampertCastilhos, Raphael Machado deSchuh, Artur Francisco SchumacherDavila, RuiDonis, Karina CarvalhoRieder, Carlos Roberto de MelloSouza, Diogo Onofre Gomes deCamey, Suzi AlvesLeotti, Vanessa BielefeldtJardim, Laura BannachPortela, Luis Valmor Cruz2018-09-25T02:34:13Z20171664-2295http://hdl.handle.net/10183/182527001049856Objectives: Spinocerebellar ataxia type 3/Machado–Joseph disease (SCA3/MJD) is a polyglutamine disorder with no current disease-modifying treatment. Conformational changes in mutant ataxin-3 trigger different pathogenic cascades, including reactive oxygen species (ROS) generation; however, the clinical relevance of oxidative stress elements as peripheral biomarkers of SCA3/MJD remains unknown. We aimed to evaluate ROS production and antioxidant defense capacity in symptomatic and presymptomatic SCA3/MJD individuals and correlate these markers with clinical and molecular data with the goal of assessing their properties as disease biomarkers. Methods: Molecularly confirmed SCA3/MJD carriers and controls were included in an exploratory case–control study. Serum ROS, measured by 2′,7′-dichlorofluorescein diacetate (DCFH-DA) as well as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) antioxidant enzyme activities, levels were assessed. Results: Fifty-eight early/moderate stage symptomatic SCA3/MJD, 12 presymptomatic SCA3/MJD, and 47 control individuals were assessed. The DCFH-DA levels in the symptomatic group were 152.82 nmol/mg of protein [95% confidence interval (CI), 82.57–223.08, p < 0.001] higher than in the control and 243.80 nmol/mg of protein (95% CI, 130.64–356.96, p < 0.001) higher than in the presymptomatic group. The SOD activity in the symptomatic group was 3 U/mg of protein (95% CI, 0.015–6.00, p = 0.048) lower than in the presymptomatic group. The GSH-Px activity in the symptomatic group was 13.96 U/mg of protein (95% CI, 5.90–22.03, p < 0.001) lower than in the control group and 20.52 U/mg of protein (95% CI, 6.79–34.24, p < 0.001) lower than in the presymptomatic group and was inversely correlated with the neurological examination score for spinocerebellar ataxias (R = −0.309, p = 0.049). Conclusion: Early/moderate stage SCA3/MJD patients presented a decreased antioxidant capacity and increased ROS generation. GSH-Px activity was the most promising oxidative stress disease biomarker in SCA3/MJD. Further longitudinal studies are necessary to identify both the roles of redox parameters in SCA3/MJD pathophysiology and as surrogate outcomes for clinical trials.application/pdfengFrontiers in neurology. Lausanne. Vol. 8, article 485 (Sept. 2017), p. 1-8Doença de Machado-JosephEstresse oxidativoEspécies reativas de oxigênioEstatística médicaSpinocerebellar ataxia type 3Machado–Joseph diseaseOxidative stressReactive oxygen speciesPolyglutamine disordersPeripheral oxidative stress biomarkers in spinocerebellar ataxia type 3/Machado–Joseph diseaseEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001049856.pdfTexto completo (inglês)application/pdf717114http://www.lume.ufrgs.br/bitstream/10183/182527/1/001049856.pdf521f956fe19341cbf63aa46a57ca2f48MD51TEXT001049856.pdf.txt001049856.pdf.txtExtracted Texttext/plain36384http://www.lume.ufrgs.br/bitstream/10183/182527/2/001049856.pdf.txt52d574cb2f4c257ae73e96d6801a6dd3MD52THUMBNAIL001049856.pdf.jpg001049856.pdf.jpgGenerated Thumbnailimage/jpeg1897http://www.lume.ufrgs.br/bitstream/10183/182527/3/001049856.pdf.jpg7158067e7d79d116adc4458c6e66c535MD5310183/1825272018-10-05 07:57:05.248oai:www.lume.ufrgs.br:10183/182527Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-05T10:57:05Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Peripheral oxidative stress biomarkers in spinocerebellar ataxia type 3/Machado–Joseph disease |
title |
Peripheral oxidative stress biomarkers in spinocerebellar ataxia type 3/Machado–Joseph disease |
spellingShingle |
Peripheral oxidative stress biomarkers in spinocerebellar ataxia type 3/Machado–Joseph disease Assis, Adriano Martimbianco de Doença de Machado-Joseph Estresse oxidativo Espécies reativas de oxigênio Estatística médica Spinocerebellar ataxia type 3 Machado–Joseph disease Oxidative stress Reactive oxygen species Polyglutamine disorders |
title_short |
Peripheral oxidative stress biomarkers in spinocerebellar ataxia type 3/Machado–Joseph disease |
title_full |
Peripheral oxidative stress biomarkers in spinocerebellar ataxia type 3/Machado–Joseph disease |
title_fullStr |
Peripheral oxidative stress biomarkers in spinocerebellar ataxia type 3/Machado–Joseph disease |
title_full_unstemmed |
Peripheral oxidative stress biomarkers in spinocerebellar ataxia type 3/Machado–Joseph disease |
title_sort |
Peripheral oxidative stress biomarkers in spinocerebellar ataxia type 3/Machado–Joseph disease |
author |
Assis, Adriano Martimbianco de |
author_facet |
Assis, Adriano Martimbianco de Saute, Jonas Alex Morales Santos, Aline Longoni dos Haas, Clarissa Branco Torrez, Vitor Rocco Brochier, Andressa Wigner Souza, Gabriele Nunes Furtado, Gabriel Vasata Gheno, Tailise Conte Russo, Aline Monte, Thais Lampert Castilhos, Raphael Machado de Schuh, Artur Francisco Schumacher Davila, Rui Donis, Karina Carvalho Rieder, Carlos Roberto de Mello Souza, Diogo Onofre Gomes de Camey, Suzi Alves Leotti, Vanessa Bielefeldt Jardim, Laura Bannach Portela, Luis Valmor Cruz |
author_role |
author |
author2 |
Saute, Jonas Alex Morales Santos, Aline Longoni dos Haas, Clarissa Branco Torrez, Vitor Rocco Brochier, Andressa Wigner Souza, Gabriele Nunes Furtado, Gabriel Vasata Gheno, Tailise Conte Russo, Aline Monte, Thais Lampert Castilhos, Raphael Machado de Schuh, Artur Francisco Schumacher Davila, Rui Donis, Karina Carvalho Rieder, Carlos Roberto de Mello Souza, Diogo Onofre Gomes de Camey, Suzi Alves Leotti, Vanessa Bielefeldt Jardim, Laura Bannach Portela, Luis Valmor Cruz |
author2_role |
author author author author author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Assis, Adriano Martimbianco de Saute, Jonas Alex Morales Santos, Aline Longoni dos Haas, Clarissa Branco Torrez, Vitor Rocco Brochier, Andressa Wigner Souza, Gabriele Nunes Furtado, Gabriel Vasata Gheno, Tailise Conte Russo, Aline Monte, Thais Lampert Castilhos, Raphael Machado de Schuh, Artur Francisco Schumacher Davila, Rui Donis, Karina Carvalho Rieder, Carlos Roberto de Mello Souza, Diogo Onofre Gomes de Camey, Suzi Alves Leotti, Vanessa Bielefeldt Jardim, Laura Bannach Portela, Luis Valmor Cruz |
dc.subject.por.fl_str_mv |
Doença de Machado-Joseph Estresse oxidativo Espécies reativas de oxigênio Estatística médica |
topic |
Doença de Machado-Joseph Estresse oxidativo Espécies reativas de oxigênio Estatística médica Spinocerebellar ataxia type 3 Machado–Joseph disease Oxidative stress Reactive oxygen species Polyglutamine disorders |
dc.subject.eng.fl_str_mv |
Spinocerebellar ataxia type 3 Machado–Joseph disease Oxidative stress Reactive oxygen species Polyglutamine disorders |
description |
Objectives: Spinocerebellar ataxia type 3/Machado–Joseph disease (SCA3/MJD) is a polyglutamine disorder with no current disease-modifying treatment. Conformational changes in mutant ataxin-3 trigger different pathogenic cascades, including reactive oxygen species (ROS) generation; however, the clinical relevance of oxidative stress elements as peripheral biomarkers of SCA3/MJD remains unknown. We aimed to evaluate ROS production and antioxidant defense capacity in symptomatic and presymptomatic SCA3/MJD individuals and correlate these markers with clinical and molecular data with the goal of assessing their properties as disease biomarkers. Methods: Molecularly confirmed SCA3/MJD carriers and controls were included in an exploratory case–control study. Serum ROS, measured by 2′,7′-dichlorofluorescein diacetate (DCFH-DA) as well as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) antioxidant enzyme activities, levels were assessed. Results: Fifty-eight early/moderate stage symptomatic SCA3/MJD, 12 presymptomatic SCA3/MJD, and 47 control individuals were assessed. The DCFH-DA levels in the symptomatic group were 152.82 nmol/mg of protein [95% confidence interval (CI), 82.57–223.08, p < 0.001] higher than in the control and 243.80 nmol/mg of protein (95% CI, 130.64–356.96, p < 0.001) higher than in the presymptomatic group. The SOD activity in the symptomatic group was 3 U/mg of protein (95% CI, 0.015–6.00, p = 0.048) lower than in the presymptomatic group. The GSH-Px activity in the symptomatic group was 13.96 U/mg of protein (95% CI, 5.90–22.03, p < 0.001) lower than in the control group and 20.52 U/mg of protein (95% CI, 6.79–34.24, p < 0.001) lower than in the presymptomatic group and was inversely correlated with the neurological examination score for spinocerebellar ataxias (R = −0.309, p = 0.049). Conclusion: Early/moderate stage SCA3/MJD patients presented a decreased antioxidant capacity and increased ROS generation. GSH-Px activity was the most promising oxidative stress disease biomarker in SCA3/MJD. Further longitudinal studies are necessary to identify both the roles of redox parameters in SCA3/MJD pathophysiology and as surrogate outcomes for clinical trials. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2018-09-25T02:34:13Z |
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Estrangeiro info:eu-repo/semantics/article |
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001049856 |
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eng |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Frontiers in neurology. Lausanne. Vol. 8, article 485 (Sept. 2017), p. 1-8 |
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