Comparison between DNA detection in trigeminal nerve ganglia and serology to detect cattle infected with bovine herpesviruses types 1 and 5

Detalhes bibliográficos
Autor(a) principal: Puentes, Rodrigo
Data de Publicação: 2016
Outros Autores: Campos, Fabrício Souza, Furtado, Agustín, Torres, Fabrício Dias, Franco, Ana Claudia, Maisonnave, Jaqueline, Roehe, Paulo Michel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/224603
Resumo: Bovine herpesviruses (BoHVs) types 1 (BoHV-1) and 5 (BoHV-5) are alphaherpesviruses of major importance to the bovine production chain. Such viruses are capable of establishing latent infections in neuronal tissues. Infected animals tend to develop a serological response to infection; however, such response—usually investigated by antibody assays in serum—may eventually not be detected in laboratory assays. Nevertheless, serological tests such as virus neutralization (VN) and various enzyme-linked immunosorbent assays (ELISAs) are widely employed to check individual or herd status of BoHV infections. The correlation between detection of antibodies and the presence of viral nucleic acids as indicatives of infection in infected cattle has not been deeply examined. In order to investigate such correlation, 248 bovine serum samples were tested by VN to BoHV-1 and BoHV-5, as well as in a widely employed (though not type-differential) gB ELISA (IDEXX IBR gB X2 Ab Test) in search for antibodies to BoHVs. Immediately after blood withdrawal, cattle were slaughtered and trigeminal ganglia (TG) excised for DNA extraction and viral nucleic acid detection (NAD) by nested PCR. Neutralizing antibodies to BoHV-1 and/or BoHV-5 were detected in 44.8% (111/248) of sera, whereas the gB ELISA detected antibodies in 51.2% (127/248) of the samples. However, genomes of either BoHV-1, BoHV-5, or both, were detected in TGs of 85.9% (213/248) of the animals. These findings reveal that the assays designed to detect antibodies to BoHV-1 and/or BoHV-5 employed here may fail to detect a significant number of latently infected animals (in this study, 35.7%). From such data, it is clear that antibody assays are poorly correlated with detection of viral genomes in BoHV-1 and BoHV-5-infected animals.
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spelling Puentes, RodrigoCampos, Fabrício SouzaFurtado, AgustínTorres, Fabrício DiasFranco, Ana ClaudiaMaisonnave, JaquelineRoehe, Paulo Michel2021-07-28T04:38:40Z20161932-6203http://hdl.handle.net/10183/224603000996097Bovine herpesviruses (BoHVs) types 1 (BoHV-1) and 5 (BoHV-5) are alphaherpesviruses of major importance to the bovine production chain. Such viruses are capable of establishing latent infections in neuronal tissues. Infected animals tend to develop a serological response to infection; however, such response—usually investigated by antibody assays in serum—may eventually not be detected in laboratory assays. Nevertheless, serological tests such as virus neutralization (VN) and various enzyme-linked immunosorbent assays (ELISAs) are widely employed to check individual or herd status of BoHV infections. The correlation between detection of antibodies and the presence of viral nucleic acids as indicatives of infection in infected cattle has not been deeply examined. In order to investigate such correlation, 248 bovine serum samples were tested by VN to BoHV-1 and BoHV-5, as well as in a widely employed (though not type-differential) gB ELISA (IDEXX IBR gB X2 Ab Test) in search for antibodies to BoHVs. Immediately after blood withdrawal, cattle were slaughtered and trigeminal ganglia (TG) excised for DNA extraction and viral nucleic acid detection (NAD) by nested PCR. Neutralizing antibodies to BoHV-1 and/or BoHV-5 were detected in 44.8% (111/248) of sera, whereas the gB ELISA detected antibodies in 51.2% (127/248) of the samples. However, genomes of either BoHV-1, BoHV-5, or both, were detected in TGs of 85.9% (213/248) of the animals. These findings reveal that the assays designed to detect antibodies to BoHV-1 and/or BoHV-5 employed here may fail to detect a significant number of latently infected animals (in this study, 35.7%). From such data, it is clear that antibody assays are poorly correlated with detection of viral genomes in BoHV-1 and BoHV-5-infected animals.application/pdfengPLoS ONE. San Francisco. Vol. 11, no. 5 (May 2016), e0155941, 9 f.Testes imunológicosInfecções por HerpesviridaeHerpesvirus bovino 1Herpesvírus bovino tipo 5Comparison between DNA detection in trigeminal nerve ganglia and serology to detect cattle infected with bovine herpesviruses types 1 and 5Estrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000996097.pdf.txt000996097.pdf.txtExtracted Texttext/plain31453http://www.lume.ufrgs.br/bitstream/10183/224603/2/000996097.pdf.txt9d6d0b8047a06ac53da315dfbf4c12c2MD52ORIGINAL000996097.pdfTexto completo (inglês)application/pdf306741http://www.lume.ufrgs.br/bitstream/10183/224603/1/000996097.pdf0a84e9f174d276935f6bed5dcd4997efMD5110183/2246032023-01-18 06:01:18.275267oai:www.lume.ufrgs.br:10183/224603Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-01-18T08:01:18Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Comparison between DNA detection in trigeminal nerve ganglia and serology to detect cattle infected with bovine herpesviruses types 1 and 5
title Comparison between DNA detection in trigeminal nerve ganglia and serology to detect cattle infected with bovine herpesviruses types 1 and 5
spellingShingle Comparison between DNA detection in trigeminal nerve ganglia and serology to detect cattle infected with bovine herpesviruses types 1 and 5
Puentes, Rodrigo
Testes imunológicos
Infecções por Herpesviridae
Herpesvirus bovino 1
Herpesvírus bovino tipo 5
title_short Comparison between DNA detection in trigeminal nerve ganglia and serology to detect cattle infected with bovine herpesviruses types 1 and 5
title_full Comparison between DNA detection in trigeminal nerve ganglia and serology to detect cattle infected with bovine herpesviruses types 1 and 5
title_fullStr Comparison between DNA detection in trigeminal nerve ganglia and serology to detect cattle infected with bovine herpesviruses types 1 and 5
title_full_unstemmed Comparison between DNA detection in trigeminal nerve ganglia and serology to detect cattle infected with bovine herpesviruses types 1 and 5
title_sort Comparison between DNA detection in trigeminal nerve ganglia and serology to detect cattle infected with bovine herpesviruses types 1 and 5
author Puentes, Rodrigo
author_facet Puentes, Rodrigo
Campos, Fabrício Souza
Furtado, Agustín
Torres, Fabrício Dias
Franco, Ana Claudia
Maisonnave, Jaqueline
Roehe, Paulo Michel
author_role author
author2 Campos, Fabrício Souza
Furtado, Agustín
Torres, Fabrício Dias
Franco, Ana Claudia
Maisonnave, Jaqueline
Roehe, Paulo Michel
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Puentes, Rodrigo
Campos, Fabrício Souza
Furtado, Agustín
Torres, Fabrício Dias
Franco, Ana Claudia
Maisonnave, Jaqueline
Roehe, Paulo Michel
dc.subject.por.fl_str_mv Testes imunológicos
Infecções por Herpesviridae
Herpesvirus bovino 1
Herpesvírus bovino tipo 5
topic Testes imunológicos
Infecções por Herpesviridae
Herpesvirus bovino 1
Herpesvírus bovino tipo 5
description Bovine herpesviruses (BoHVs) types 1 (BoHV-1) and 5 (BoHV-5) are alphaherpesviruses of major importance to the bovine production chain. Such viruses are capable of establishing latent infections in neuronal tissues. Infected animals tend to develop a serological response to infection; however, such response—usually investigated by antibody assays in serum—may eventually not be detected in laboratory assays. Nevertheless, serological tests such as virus neutralization (VN) and various enzyme-linked immunosorbent assays (ELISAs) are widely employed to check individual or herd status of BoHV infections. The correlation between detection of antibodies and the presence of viral nucleic acids as indicatives of infection in infected cattle has not been deeply examined. In order to investigate such correlation, 248 bovine serum samples were tested by VN to BoHV-1 and BoHV-5, as well as in a widely employed (though not type-differential) gB ELISA (IDEXX IBR gB X2 Ab Test) in search for antibodies to BoHVs. Immediately after blood withdrawal, cattle were slaughtered and trigeminal ganglia (TG) excised for DNA extraction and viral nucleic acid detection (NAD) by nested PCR. Neutralizing antibodies to BoHV-1 and/or BoHV-5 were detected in 44.8% (111/248) of sera, whereas the gB ELISA detected antibodies in 51.2% (127/248) of the samples. However, genomes of either BoHV-1, BoHV-5, or both, were detected in TGs of 85.9% (213/248) of the animals. These findings reveal that the assays designed to detect antibodies to BoHV-1 and/or BoHV-5 employed here may fail to detect a significant number of latently infected animals (in this study, 35.7%). From such data, it is clear that antibody assays are poorly correlated with detection of viral genomes in BoHV-1 and BoHV-5-infected animals.
publishDate 2016
dc.date.issued.fl_str_mv 2016
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dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
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dc.relation.ispartof.pt_BR.fl_str_mv PLoS ONE. San Francisco. Vol. 11, no. 5 (May 2016), e0155941, 9 f.
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