Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) : characterization and comparison
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/225542 |
Resumo: | Introduction: Avian pathogenic E. coli (APEC) and uropathogenic E. coli (UPEC) are responsible for avian colibacillosis and human urinary tract infections, respectively. There are genetic similarities between the APEC and UPEC pathotypes, suggesting the APEC strains could be a potential reservoir of virulence and antimicrobial-resistance genes for the UPEC strains. This study aimed to characterize and compare APEC and UPEC strains regarding the phylogroup classification, pathogenicity and antimicrobial susceptibility. Methodology: A total of 238 APEC and 184 UPEC strains were selected and characterized. The strains were assayed for antimicrobial susceptibility and classified into phylogenetic groups using a multiplex-PCR protocol. In addition, the APEC strains had previously been classified according to their in vivo pathogenicity. Results: The results showed that both pathotypes had variation in their susceptibility to most of the antimicrobial agents evaluated, with few strains classified as multidrug resistant. The highest resistance rate for both pathotypes was to amoxicillin. Classifying the APEC and UPEC strains into phylogenetic groups determined that the most frequently frequencies were for groups D and B2, respectively. These results reflect the pathogenic potential of these strains, as all the UPEC strains were isolated from unhealthy patients, and most of the APEC strains were previously classified as pathogenic. Conclusions: The results indicate that distribution into phylogenetic groups provided, in part, similar classification to those of in vivo pathogenicity index, as it was possible to adequately differentiate most of the pathogenic and commensal or low-pathogenicity bacteria. However, no relationship could be found between the specific antimicrobial agents and pathogenicity or phylogenetic group for either pathotype. |
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Rocha, Daniela Tonini daSalle, Felipe de OliveiraBorges, Karen ApellanisFurian, Thales QuediNascimento, Vladimir Pinheiro doMoraes, Hamilton Luiz de SouzaSalle, Carlos Tadeu Pippi2021-08-11T04:48:17Z20211972-2680http://hdl.handle.net/10183/225542001130048Introduction: Avian pathogenic E. coli (APEC) and uropathogenic E. coli (UPEC) are responsible for avian colibacillosis and human urinary tract infections, respectively. There are genetic similarities between the APEC and UPEC pathotypes, suggesting the APEC strains could be a potential reservoir of virulence and antimicrobial-resistance genes for the UPEC strains. This study aimed to characterize and compare APEC and UPEC strains regarding the phylogroup classification, pathogenicity and antimicrobial susceptibility. Methodology: A total of 238 APEC and 184 UPEC strains were selected and characterized. The strains were assayed for antimicrobial susceptibility and classified into phylogenetic groups using a multiplex-PCR protocol. In addition, the APEC strains had previously been classified according to their in vivo pathogenicity. Results: The results showed that both pathotypes had variation in their susceptibility to most of the antimicrobial agents evaluated, with few strains classified as multidrug resistant. The highest resistance rate for both pathotypes was to amoxicillin. Classifying the APEC and UPEC strains into phylogenetic groups determined that the most frequently frequencies were for groups D and B2, respectively. These results reflect the pathogenic potential of these strains, as all the UPEC strains were isolated from unhealthy patients, and most of the APEC strains were previously classified as pathogenic. Conclusions: The results indicate that distribution into phylogenetic groups provided, in part, similar classification to those of in vivo pathogenicity index, as it was possible to adequately differentiate most of the pathogenic and commensal or low-pathogenicity bacteria. However, no relationship could be found between the specific antimicrobial agents and pathogenicity or phylogenetic group for either pathotype.application/pdfengJournal of Infection in Developing Countries. [Sassari, Italy]. Vol. 15, no. 7 (July 2021), p. 962-971Escherichia coli patogênia aviária (APEC)Escherichia coli uropatogênica (UPEC)Resistência a antimicrobianosPatogenicidadeFilogeniaAPECUPECPathogenicityAntimicrobial susceptibilityPhylogenetic groupsAvian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) : characterization and comparisonEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001130048.pdf.txt001130048.pdf.txtExtracted Texttext/plain48322http://www.lume.ufrgs.br/bitstream/10183/225542/2/001130048.pdf.txt905aadfed3c2d80eed21f9b32db3f680MD52ORIGINAL001130048.pdfTexto completo (inglês)application/pdf594877http://www.lume.ufrgs.br/bitstream/10183/225542/1/001130048.pdf5d4769cf9ca35f1f92567844bae45fd6MD5110183/2255422021-08-18 04:44:18.20129oai:www.lume.ufrgs.br:10183/225542Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-08-18T07:44:18Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) : characterization and comparison |
title |
Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) : characterization and comparison |
spellingShingle |
Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) : characterization and comparison Rocha, Daniela Tonini da Escherichia coli patogênia aviária (APEC) Escherichia coli uropatogênica (UPEC) Resistência a antimicrobianos Patogenicidade Filogenia APEC UPEC Pathogenicity Antimicrobial susceptibility Phylogenetic groups |
title_short |
Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) : characterization and comparison |
title_full |
Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) : characterization and comparison |
title_fullStr |
Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) : characterization and comparison |
title_full_unstemmed |
Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) : characterization and comparison |
title_sort |
Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) : characterization and comparison |
author |
Rocha, Daniela Tonini da |
author_facet |
Rocha, Daniela Tonini da Salle, Felipe de Oliveira Borges, Karen Apellanis Furian, Thales Quedi Nascimento, Vladimir Pinheiro do Moraes, Hamilton Luiz de Souza Salle, Carlos Tadeu Pippi |
author_role |
author |
author2 |
Salle, Felipe de Oliveira Borges, Karen Apellanis Furian, Thales Quedi Nascimento, Vladimir Pinheiro do Moraes, Hamilton Luiz de Souza Salle, Carlos Tadeu Pippi |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Rocha, Daniela Tonini da Salle, Felipe de Oliveira Borges, Karen Apellanis Furian, Thales Quedi Nascimento, Vladimir Pinheiro do Moraes, Hamilton Luiz de Souza Salle, Carlos Tadeu Pippi |
dc.subject.por.fl_str_mv |
Escherichia coli patogênia aviária (APEC) Escherichia coli uropatogênica (UPEC) Resistência a antimicrobianos Patogenicidade Filogenia |
topic |
Escherichia coli patogênia aviária (APEC) Escherichia coli uropatogênica (UPEC) Resistência a antimicrobianos Patogenicidade Filogenia APEC UPEC Pathogenicity Antimicrobial susceptibility Phylogenetic groups |
dc.subject.eng.fl_str_mv |
APEC UPEC Pathogenicity Antimicrobial susceptibility Phylogenetic groups |
description |
Introduction: Avian pathogenic E. coli (APEC) and uropathogenic E. coli (UPEC) are responsible for avian colibacillosis and human urinary tract infections, respectively. There are genetic similarities between the APEC and UPEC pathotypes, suggesting the APEC strains could be a potential reservoir of virulence and antimicrobial-resistance genes for the UPEC strains. This study aimed to characterize and compare APEC and UPEC strains regarding the phylogroup classification, pathogenicity and antimicrobial susceptibility. Methodology: A total of 238 APEC and 184 UPEC strains were selected and characterized. The strains were assayed for antimicrobial susceptibility and classified into phylogenetic groups using a multiplex-PCR protocol. In addition, the APEC strains had previously been classified according to their in vivo pathogenicity. Results: The results showed that both pathotypes had variation in their susceptibility to most of the antimicrobial agents evaluated, with few strains classified as multidrug resistant. The highest resistance rate for both pathotypes was to amoxicillin. Classifying the APEC and UPEC strains into phylogenetic groups determined that the most frequently frequencies were for groups D and B2, respectively. These results reflect the pathogenic potential of these strains, as all the UPEC strains were isolated from unhealthy patients, and most of the APEC strains were previously classified as pathogenic. Conclusions: The results indicate that distribution into phylogenetic groups provided, in part, similar classification to those of in vivo pathogenicity index, as it was possible to adequately differentiate most of the pathogenic and commensal or low-pathogenicity bacteria. However, no relationship could be found between the specific antimicrobial agents and pathogenicity or phylogenetic group for either pathotype. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-08-11T04:48:17Z |
dc.date.issued.fl_str_mv |
2021 |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://hdl.handle.net/10183/225542 |
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1972-2680 |
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001130048 |
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http://hdl.handle.net/10183/225542 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Journal of Infection in Developing Countries. [Sassari, Italy]. Vol. 15, no. 7 (July 2021), p. 962-971 |
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