Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) : characterization and comparison

Detalhes bibliográficos
Autor(a) principal: Rocha, Daniela Tonini da
Data de Publicação: 2021
Outros Autores: Salle, Felipe de Oliveira, Borges, Karen Apellanis, Furian, Thales Quedi, Nascimento, Vladimir Pinheiro do, Moraes, Hamilton Luiz de Souza, Salle, Carlos Tadeu Pippi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/225542
Resumo: Introduction: Avian pathogenic E. coli (APEC) and uropathogenic E. coli (UPEC) are responsible for avian colibacillosis and human urinary tract infections, respectively. There are genetic similarities between the APEC and UPEC pathotypes, suggesting the APEC strains could be a potential reservoir of virulence and antimicrobial-resistance genes for the UPEC strains. This study aimed to characterize and compare APEC and UPEC strains regarding the phylogroup classification, pathogenicity and antimicrobial susceptibility. Methodology: A total of 238 APEC and 184 UPEC strains were selected and characterized. The strains were assayed for antimicrobial susceptibility and classified into phylogenetic groups using a multiplex-PCR protocol. In addition, the APEC strains had previously been classified according to their in vivo pathogenicity. Results: The results showed that both pathotypes had variation in their susceptibility to most of the antimicrobial agents evaluated, with few strains classified as multidrug resistant. The highest resistance rate for both pathotypes was to amoxicillin. Classifying the APEC and UPEC strains into phylogenetic groups determined that the most frequently frequencies were for groups D and B2, respectively. These results reflect the pathogenic potential of these strains, as all the UPEC strains were isolated from unhealthy patients, and most of the APEC strains were previously classified as pathogenic. Conclusions: The results indicate that distribution into phylogenetic groups provided, in part, similar classification to those of in vivo pathogenicity index, as it was possible to adequately differentiate most of the pathogenic and commensal or low-pathogenicity bacteria. However, no relationship could be found between the specific antimicrobial agents and pathogenicity or phylogenetic group for either pathotype.
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spelling Rocha, Daniela Tonini daSalle, Felipe de OliveiraBorges, Karen ApellanisFurian, Thales QuediNascimento, Vladimir Pinheiro doMoraes, Hamilton Luiz de SouzaSalle, Carlos Tadeu Pippi2021-08-11T04:48:17Z20211972-2680http://hdl.handle.net/10183/225542001130048Introduction: Avian pathogenic E. coli (APEC) and uropathogenic E. coli (UPEC) are responsible for avian colibacillosis and human urinary tract infections, respectively. There are genetic similarities between the APEC and UPEC pathotypes, suggesting the APEC strains could be a potential reservoir of virulence and antimicrobial-resistance genes for the UPEC strains. This study aimed to characterize and compare APEC and UPEC strains regarding the phylogroup classification, pathogenicity and antimicrobial susceptibility. Methodology: A total of 238 APEC and 184 UPEC strains were selected and characterized. The strains were assayed for antimicrobial susceptibility and classified into phylogenetic groups using a multiplex-PCR protocol. In addition, the APEC strains had previously been classified according to their in vivo pathogenicity. Results: The results showed that both pathotypes had variation in their susceptibility to most of the antimicrobial agents evaluated, with few strains classified as multidrug resistant. The highest resistance rate for both pathotypes was to amoxicillin. Classifying the APEC and UPEC strains into phylogenetic groups determined that the most frequently frequencies were for groups D and B2, respectively. These results reflect the pathogenic potential of these strains, as all the UPEC strains were isolated from unhealthy patients, and most of the APEC strains were previously classified as pathogenic. Conclusions: The results indicate that distribution into phylogenetic groups provided, in part, similar classification to those of in vivo pathogenicity index, as it was possible to adequately differentiate most of the pathogenic and commensal or low-pathogenicity bacteria. However, no relationship could be found between the specific antimicrobial agents and pathogenicity or phylogenetic group for either pathotype.application/pdfengJournal of Infection in Developing Countries. [Sassari, Italy]. Vol. 15, no. 7 (July 2021), p. 962-971Escherichia coli patogênia aviária (APEC)Escherichia coli uropatogênica (UPEC)Resistência a antimicrobianosPatogenicidadeFilogeniaAPECUPECPathogenicityAntimicrobial susceptibilityPhylogenetic groupsAvian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) : characterization and comparisonEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001130048.pdf.txt001130048.pdf.txtExtracted Texttext/plain48322http://www.lume.ufrgs.br/bitstream/10183/225542/2/001130048.pdf.txt905aadfed3c2d80eed21f9b32db3f680MD52ORIGINAL001130048.pdfTexto completo (inglês)application/pdf594877http://www.lume.ufrgs.br/bitstream/10183/225542/1/001130048.pdf5d4769cf9ca35f1f92567844bae45fd6MD5110183/2255422021-08-18 04:44:18.20129oai:www.lume.ufrgs.br:10183/225542Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-08-18T07:44:18Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) : characterization and comparison
title Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) : characterization and comparison
spellingShingle Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) : characterization and comparison
Rocha, Daniela Tonini da
Escherichia coli patogênia aviária (APEC)
Escherichia coli uropatogênica (UPEC)
Resistência a antimicrobianos
Patogenicidade
Filogenia
APEC
UPEC
Pathogenicity
Antimicrobial susceptibility
Phylogenetic groups
title_short Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) : characterization and comparison
title_full Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) : characterization and comparison
title_fullStr Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) : characterization and comparison
title_full_unstemmed Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) : characterization and comparison
title_sort Avian pathogenic Escherichia coli (APEC) and uropathogenic Escherichia coli (UPEC) : characterization and comparison
author Rocha, Daniela Tonini da
author_facet Rocha, Daniela Tonini da
Salle, Felipe de Oliveira
Borges, Karen Apellanis
Furian, Thales Quedi
Nascimento, Vladimir Pinheiro do
Moraes, Hamilton Luiz de Souza
Salle, Carlos Tadeu Pippi
author_role author
author2 Salle, Felipe de Oliveira
Borges, Karen Apellanis
Furian, Thales Quedi
Nascimento, Vladimir Pinheiro do
Moraes, Hamilton Luiz de Souza
Salle, Carlos Tadeu Pippi
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rocha, Daniela Tonini da
Salle, Felipe de Oliveira
Borges, Karen Apellanis
Furian, Thales Quedi
Nascimento, Vladimir Pinheiro do
Moraes, Hamilton Luiz de Souza
Salle, Carlos Tadeu Pippi
dc.subject.por.fl_str_mv Escherichia coli patogênia aviária (APEC)
Escherichia coli uropatogênica (UPEC)
Resistência a antimicrobianos
Patogenicidade
Filogenia
topic Escherichia coli patogênia aviária (APEC)
Escherichia coli uropatogênica (UPEC)
Resistência a antimicrobianos
Patogenicidade
Filogenia
APEC
UPEC
Pathogenicity
Antimicrobial susceptibility
Phylogenetic groups
dc.subject.eng.fl_str_mv APEC
UPEC
Pathogenicity
Antimicrobial susceptibility
Phylogenetic groups
description Introduction: Avian pathogenic E. coli (APEC) and uropathogenic E. coli (UPEC) are responsible for avian colibacillosis and human urinary tract infections, respectively. There are genetic similarities between the APEC and UPEC pathotypes, suggesting the APEC strains could be a potential reservoir of virulence and antimicrobial-resistance genes for the UPEC strains. This study aimed to characterize and compare APEC and UPEC strains regarding the phylogroup classification, pathogenicity and antimicrobial susceptibility. Methodology: A total of 238 APEC and 184 UPEC strains were selected and characterized. The strains were assayed for antimicrobial susceptibility and classified into phylogenetic groups using a multiplex-PCR protocol. In addition, the APEC strains had previously been classified according to their in vivo pathogenicity. Results: The results showed that both pathotypes had variation in their susceptibility to most of the antimicrobial agents evaluated, with few strains classified as multidrug resistant. The highest resistance rate for both pathotypes was to amoxicillin. Classifying the APEC and UPEC strains into phylogenetic groups determined that the most frequently frequencies were for groups D and B2, respectively. These results reflect the pathogenic potential of these strains, as all the UPEC strains were isolated from unhealthy patients, and most of the APEC strains were previously classified as pathogenic. Conclusions: The results indicate that distribution into phylogenetic groups provided, in part, similar classification to those of in vivo pathogenicity index, as it was possible to adequately differentiate most of the pathogenic and commensal or low-pathogenicity bacteria. However, no relationship could be found between the specific antimicrobial agents and pathogenicity or phylogenetic group for either pathotype.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-08-11T04:48:17Z
dc.date.issued.fl_str_mv 2021
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dc.identifier.issn.pt_BR.fl_str_mv 1972-2680
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dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Journal of Infection in Developing Countries. [Sassari, Italy]. Vol. 15, no. 7 (July 2021), p. 962-971
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