Antifungal susceptibility of yeasts isolated from anthropogenic watershed
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/194657 |
Resumo: | Yeasts are unicellular fungi widespread in the environment, and studies suggest that there is a positive correlation between yeast and polluted aquatic environments. The aim of this study was to analyze the diversity and resistance of yeasts isolated from water samples collected along the Arroio Dilúvio in Porto Alegre to antifungals. Yeast isolates from the Arroio Dilúvio were subjected to susceptibility assays against antifungals using the minimum inhibitory concentration (MIC) test, and amplification of the ITS1- 5.8S-IT2 region; in addition, the ITS-5.8S region was sequenced for some of the isolates. The amplification product was subjected to PCR-RFLP, and the restriction profile allowed the construction of a dendrogram. Susceptibility tests showed a high prevalence of resistance to azole antifungals, where 16.8% of the isolates had a resistance profile to amphotericin B. The sequence analysis allowed the identification of Candida species, including potentially pathogenic species, and species of the Debaryomyces spp. The resistance to antifungals in yeasts isolated from Arroio Dilúvio reinforces the importance of studies of environmental microbiota, and indicates that environmental degradation influences the phenotype displayed. |
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Milanezi, Ana Carolina MedeirosWitusk, João Paulo DuarteVan der Sand, Sueli Terezinha2019-05-28T02:36:59Z20190001-3765http://hdl.handle.net/10183/194657001088363Yeasts are unicellular fungi widespread in the environment, and studies suggest that there is a positive correlation between yeast and polluted aquatic environments. The aim of this study was to analyze the diversity and resistance of yeasts isolated from water samples collected along the Arroio Dilúvio in Porto Alegre to antifungals. Yeast isolates from the Arroio Dilúvio were subjected to susceptibility assays against antifungals using the minimum inhibitory concentration (MIC) test, and amplification of the ITS1- 5.8S-IT2 region; in addition, the ITS-5.8S region was sequenced for some of the isolates. The amplification product was subjected to PCR-RFLP, and the restriction profile allowed the construction of a dendrogram. Susceptibility tests showed a high prevalence of resistance to azole antifungals, where 16.8% of the isolates had a resistance profile to amphotericin B. The sequence analysis allowed the identification of Candida species, including potentially pathogenic species, and species of the Debaryomyces spp. The resistance to antifungals in yeasts isolated from Arroio Dilúvio reinforces the importance of studies of environmental microbiota, and indicates that environmental degradation influences the phenotype displayed.application/pdfengAnais da Academia Brasileira de Ciências. Rio de Janeiro, RJ. Vol. 91, n. 1 (2019), e20170369, 12 p.AntifúngicosLevedurasPoluição da águaResistência microbiana a medicamentosYeastResistanceWatershedAntifungalAntifungal susceptibility of yeasts isolated from anthropogenic watershedinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001088363.pdf.txt001088363.pdf.txtExtracted Texttext/plain40691http://www.lume.ufrgs.br/bitstream/10183/194657/2/001088363.pdf.txtd1c892e48909f8011060ef09c3c30b1eMD52ORIGINAL001088363.pdfTexto completo (inglês)application/pdf418111http://www.lume.ufrgs.br/bitstream/10183/194657/1/001088363.pdf682437950d97874aaa95abd66eaa7a7cMD5110183/1946572023-07-08 03:34:35.784345oai:www.lume.ufrgs.br:10183/194657Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-07-08T06:34:35Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Antifungal susceptibility of yeasts isolated from anthropogenic watershed |
title |
Antifungal susceptibility of yeasts isolated from anthropogenic watershed |
spellingShingle |
Antifungal susceptibility of yeasts isolated from anthropogenic watershed Milanezi, Ana Carolina Medeiros Antifúngicos Leveduras Poluição da água Resistência microbiana a medicamentos Yeast Resistance Watershed Antifungal |
title_short |
Antifungal susceptibility of yeasts isolated from anthropogenic watershed |
title_full |
Antifungal susceptibility of yeasts isolated from anthropogenic watershed |
title_fullStr |
Antifungal susceptibility of yeasts isolated from anthropogenic watershed |
title_full_unstemmed |
Antifungal susceptibility of yeasts isolated from anthropogenic watershed |
title_sort |
Antifungal susceptibility of yeasts isolated from anthropogenic watershed |
author |
Milanezi, Ana Carolina Medeiros |
author_facet |
Milanezi, Ana Carolina Medeiros Witusk, João Paulo Duarte Van der Sand, Sueli Terezinha |
author_role |
author |
author2 |
Witusk, João Paulo Duarte Van der Sand, Sueli Terezinha |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Milanezi, Ana Carolina Medeiros Witusk, João Paulo Duarte Van der Sand, Sueli Terezinha |
dc.subject.por.fl_str_mv |
Antifúngicos Leveduras Poluição da água Resistência microbiana a medicamentos |
topic |
Antifúngicos Leveduras Poluição da água Resistência microbiana a medicamentos Yeast Resistance Watershed Antifungal |
dc.subject.eng.fl_str_mv |
Yeast Resistance Watershed Antifungal |
description |
Yeasts are unicellular fungi widespread in the environment, and studies suggest that there is a positive correlation between yeast and polluted aquatic environments. The aim of this study was to analyze the diversity and resistance of yeasts isolated from water samples collected along the Arroio Dilúvio in Porto Alegre to antifungals. Yeast isolates from the Arroio Dilúvio were subjected to susceptibility assays against antifungals using the minimum inhibitory concentration (MIC) test, and amplification of the ITS1- 5.8S-IT2 region; in addition, the ITS-5.8S region was sequenced for some of the isolates. The amplification product was subjected to PCR-RFLP, and the restriction profile allowed the construction of a dendrogram. Susceptibility tests showed a high prevalence of resistance to azole antifungals, where 16.8% of the isolates had a resistance profile to amphotericin B. The sequence analysis allowed the identification of Candida species, including potentially pathogenic species, and species of the Debaryomyces spp. The resistance to antifungals in yeasts isolated from Arroio Dilúvio reinforces the importance of studies of environmental microbiota, and indicates that environmental degradation influences the phenotype displayed. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-05-28T02:36:59Z |
dc.date.issued.fl_str_mv |
2019 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/194657 |
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0001-3765 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001088363 |
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0001-3765 001088363 |
url |
http://hdl.handle.net/10183/194657 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Anais da Academia Brasileira de Ciências. Rio de Janeiro, RJ. Vol. 91, n. 1 (2019), e20170369, 12 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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