Flow cytometry evaluation of CD14/CD16 monocyte subpopulations in systemic sclerosis patients : a cross sectional controlled study

Detalhes bibliográficos
Autor(a) principal: Schneider, Laiana
Data de Publicação: 2021
Outros Autores: Marcondes, Natália Aydos, Hax, Vanessa, Moreira, Isadora Flesch da Silva, Ueda, Carolina Yuka, Piovesan, Rafaella Romeiro, Xavier, Ricardo Machado, Chakr, Rafael Mendonça da Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/229342
Resumo: Background: Systemic sclerosis (SSc) is a chronic autoimmune disease characterized by vasculopathy and fibrosis, which can be subclassified into diffuse cutaneous (dSSc) and limited cutaneous (lSSc) subtypes. Previous studies suggest that an increase in monocytes can be a hallmark of various inflammatory diseases, including SSc. Our aim was to evaluate circulating blood monocyte subpopulations (classical, intermediate and non-classical) of SSc patients and their possible association with disease manifestations. Methods: Fifty consecutive patients fulfilling the 2013 ACR/EULAR classification criteria for SSc were included in a cross-sectional study. Monocyte subpopulations were identified based on their expression of CD64, CD14 and CD16, evaluated by flow cytometry, and were correlated with the clinical characteristics of the patients; furthermore, the expression of HLA-DR, CD163, CD169 and CD206 in the monocytes was studied. Thirty-eight age- and sex-matched healthy individuals were recruited as a control group. Results: SSc patients had an increased number of circulating peripheral blood monocytes with an activated phenotypic profile compared to healthy subjects. Absolute counts of CD16+ (intermediary and non-classical) monocyte subpopulations were higher in SSc patients. There was no association between monocyte subpopulations and the clinical manifestations evaluated. Conclusion: We identified higher counts of all monocyte subpopulations in SSc patients compared to the control group. There was no association between monocyte subpopulations and major fibrotic manifestations. CD169 was shown to be more representative in dSSc, being a promising marker for differentiating disease subtypes.
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spelling Schneider, LaianaMarcondes, Natália AydosHax, VanessaMoreira, Isadora Flesch da SilvaUeda, Carolina YukaPiovesan, Rafaella RomeiroXavier, Ricardo MachadoChakr, Rafael Mendonça da Silva2021-09-01T04:27:21Z20212523-3106http://hdl.handle.net/10183/229342001130271Background: Systemic sclerosis (SSc) is a chronic autoimmune disease characterized by vasculopathy and fibrosis, which can be subclassified into diffuse cutaneous (dSSc) and limited cutaneous (lSSc) subtypes. Previous studies suggest that an increase in monocytes can be a hallmark of various inflammatory diseases, including SSc. Our aim was to evaluate circulating blood monocyte subpopulations (classical, intermediate and non-classical) of SSc patients and their possible association with disease manifestations. Methods: Fifty consecutive patients fulfilling the 2013 ACR/EULAR classification criteria for SSc were included in a cross-sectional study. Monocyte subpopulations were identified based on their expression of CD64, CD14 and CD16, evaluated by flow cytometry, and were correlated with the clinical characteristics of the patients; furthermore, the expression of HLA-DR, CD163, CD169 and CD206 in the monocytes was studied. Thirty-eight age- and sex-matched healthy individuals were recruited as a control group. Results: SSc patients had an increased number of circulating peripheral blood monocytes with an activated phenotypic profile compared to healthy subjects. Absolute counts of CD16+ (intermediary and non-classical) monocyte subpopulations were higher in SSc patients. There was no association between monocyte subpopulations and the clinical manifestations evaluated. Conclusion: We identified higher counts of all monocyte subpopulations in SSc patients compared to the control group. There was no association between monocyte subpopulations and major fibrotic manifestations. CD169 was shown to be more representative in dSSc, being a promising marker for differentiating disease subtypes.application/pdfengAdvances in rheumatology. São Paulo. Vol. 61 (2021), 27, 11 p.Escleroderma sistêmicoMonocitosCitometria de fluxoSystemic sclerosisMonocytesMonocyte subpopulationsFlow cytometryPathogenesisFlow cytometry evaluation of CD14/CD16 monocyte subpopulations in systemic sclerosis patients : a cross sectional controlled studyinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001130271.pdf.txt001130271.pdf.txtExtracted Texttext/plain43490http://www.lume.ufrgs.br/bitstream/10183/229342/2/001130271.pdf.txt920be9fff297a7a2eaa15f82608ec157MD52ORIGINAL001130271.pdfTexto completo (inglês)application/pdf1618535http://www.lume.ufrgs.br/bitstream/10183/229342/1/001130271.pdfeb98e011a4dae1a4ab72d8ce60f6bfe6MD5110183/2293422023-05-24 03:25:44.006492oai:www.lume.ufrgs.br:10183/229342Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-05-24T06:25:44Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Flow cytometry evaluation of CD14/CD16 monocyte subpopulations in systemic sclerosis patients : a cross sectional controlled study
title Flow cytometry evaluation of CD14/CD16 monocyte subpopulations in systemic sclerosis patients : a cross sectional controlled study
spellingShingle Flow cytometry evaluation of CD14/CD16 monocyte subpopulations in systemic sclerosis patients : a cross sectional controlled study
Schneider, Laiana
Escleroderma sistêmico
Monocitos
Citometria de fluxo
Systemic sclerosis
Monocytes
Monocyte subpopulations
Flow cytometry
Pathogenesis
title_short Flow cytometry evaluation of CD14/CD16 monocyte subpopulations in systemic sclerosis patients : a cross sectional controlled study
title_full Flow cytometry evaluation of CD14/CD16 monocyte subpopulations in systemic sclerosis patients : a cross sectional controlled study
title_fullStr Flow cytometry evaluation of CD14/CD16 monocyte subpopulations in systemic sclerosis patients : a cross sectional controlled study
title_full_unstemmed Flow cytometry evaluation of CD14/CD16 monocyte subpopulations in systemic sclerosis patients : a cross sectional controlled study
title_sort Flow cytometry evaluation of CD14/CD16 monocyte subpopulations in systemic sclerosis patients : a cross sectional controlled study
author Schneider, Laiana
author_facet Schneider, Laiana
Marcondes, Natália Aydos
Hax, Vanessa
Moreira, Isadora Flesch da Silva
Ueda, Carolina Yuka
Piovesan, Rafaella Romeiro
Xavier, Ricardo Machado
Chakr, Rafael Mendonça da Silva
author_role author
author2 Marcondes, Natália Aydos
Hax, Vanessa
Moreira, Isadora Flesch da Silva
Ueda, Carolina Yuka
Piovesan, Rafaella Romeiro
Xavier, Ricardo Machado
Chakr, Rafael Mendonça da Silva
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Schneider, Laiana
Marcondes, Natália Aydos
Hax, Vanessa
Moreira, Isadora Flesch da Silva
Ueda, Carolina Yuka
Piovesan, Rafaella Romeiro
Xavier, Ricardo Machado
Chakr, Rafael Mendonça da Silva
dc.subject.por.fl_str_mv Escleroderma sistêmico
Monocitos
Citometria de fluxo
topic Escleroderma sistêmico
Monocitos
Citometria de fluxo
Systemic sclerosis
Monocytes
Monocyte subpopulations
Flow cytometry
Pathogenesis
dc.subject.eng.fl_str_mv Systemic sclerosis
Monocytes
Monocyte subpopulations
Flow cytometry
Pathogenesis
description Background: Systemic sclerosis (SSc) is a chronic autoimmune disease characterized by vasculopathy and fibrosis, which can be subclassified into diffuse cutaneous (dSSc) and limited cutaneous (lSSc) subtypes. Previous studies suggest that an increase in monocytes can be a hallmark of various inflammatory diseases, including SSc. Our aim was to evaluate circulating blood monocyte subpopulations (classical, intermediate and non-classical) of SSc patients and their possible association with disease manifestations. Methods: Fifty consecutive patients fulfilling the 2013 ACR/EULAR classification criteria for SSc were included in a cross-sectional study. Monocyte subpopulations were identified based on their expression of CD64, CD14 and CD16, evaluated by flow cytometry, and were correlated with the clinical characteristics of the patients; furthermore, the expression of HLA-DR, CD163, CD169 and CD206 in the monocytes was studied. Thirty-eight age- and sex-matched healthy individuals were recruited as a control group. Results: SSc patients had an increased number of circulating peripheral blood monocytes with an activated phenotypic profile compared to healthy subjects. Absolute counts of CD16+ (intermediary and non-classical) monocyte subpopulations were higher in SSc patients. There was no association between monocyte subpopulations and the clinical manifestations evaluated. Conclusion: We identified higher counts of all monocyte subpopulations in SSc patients compared to the control group. There was no association between monocyte subpopulations and major fibrotic manifestations. CD169 was shown to be more representative in dSSc, being a promising marker for differentiating disease subtypes.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-09-01T04:27:21Z
dc.date.issued.fl_str_mv 2021
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dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Advances in rheumatology. São Paulo. Vol. 61 (2021), 27, 11 p.
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reponame_str Repositório Institucional da UFRGS
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