Flow cytometry evaluation of CD14/CD16 monocyte subpopulations in systemic sclerosis patients : a cross sectional controlled study
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/229342 |
Resumo: | Background: Systemic sclerosis (SSc) is a chronic autoimmune disease characterized by vasculopathy and fibrosis, which can be subclassified into diffuse cutaneous (dSSc) and limited cutaneous (lSSc) subtypes. Previous studies suggest that an increase in monocytes can be a hallmark of various inflammatory diseases, including SSc. Our aim was to evaluate circulating blood monocyte subpopulations (classical, intermediate and non-classical) of SSc patients and their possible association with disease manifestations. Methods: Fifty consecutive patients fulfilling the 2013 ACR/EULAR classification criteria for SSc were included in a cross-sectional study. Monocyte subpopulations were identified based on their expression of CD64, CD14 and CD16, evaluated by flow cytometry, and were correlated with the clinical characteristics of the patients; furthermore, the expression of HLA-DR, CD163, CD169 and CD206 in the monocytes was studied. Thirty-eight age- and sex-matched healthy individuals were recruited as a control group. Results: SSc patients had an increased number of circulating peripheral blood monocytes with an activated phenotypic profile compared to healthy subjects. Absolute counts of CD16+ (intermediary and non-classical) monocyte subpopulations were higher in SSc patients. There was no association between monocyte subpopulations and the clinical manifestations evaluated. Conclusion: We identified higher counts of all monocyte subpopulations in SSc patients compared to the control group. There was no association between monocyte subpopulations and major fibrotic manifestations. CD169 was shown to be more representative in dSSc, being a promising marker for differentiating disease subtypes. |
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Schneider, LaianaMarcondes, Natália AydosHax, VanessaMoreira, Isadora Flesch da SilvaUeda, Carolina YukaPiovesan, Rafaella RomeiroXavier, Ricardo MachadoChakr, Rafael Mendonça da Silva2021-09-01T04:27:21Z20212523-3106http://hdl.handle.net/10183/229342001130271Background: Systemic sclerosis (SSc) is a chronic autoimmune disease characterized by vasculopathy and fibrosis, which can be subclassified into diffuse cutaneous (dSSc) and limited cutaneous (lSSc) subtypes. Previous studies suggest that an increase in monocytes can be a hallmark of various inflammatory diseases, including SSc. Our aim was to evaluate circulating blood monocyte subpopulations (classical, intermediate and non-classical) of SSc patients and their possible association with disease manifestations. Methods: Fifty consecutive patients fulfilling the 2013 ACR/EULAR classification criteria for SSc were included in a cross-sectional study. Monocyte subpopulations were identified based on their expression of CD64, CD14 and CD16, evaluated by flow cytometry, and were correlated with the clinical characteristics of the patients; furthermore, the expression of HLA-DR, CD163, CD169 and CD206 in the monocytes was studied. Thirty-eight age- and sex-matched healthy individuals were recruited as a control group. Results: SSc patients had an increased number of circulating peripheral blood monocytes with an activated phenotypic profile compared to healthy subjects. Absolute counts of CD16+ (intermediary and non-classical) monocyte subpopulations were higher in SSc patients. There was no association between monocyte subpopulations and the clinical manifestations evaluated. Conclusion: We identified higher counts of all monocyte subpopulations in SSc patients compared to the control group. There was no association between monocyte subpopulations and major fibrotic manifestations. CD169 was shown to be more representative in dSSc, being a promising marker for differentiating disease subtypes.application/pdfengAdvances in rheumatology. São Paulo. Vol. 61 (2021), 27, 11 p.Escleroderma sistêmicoMonocitosCitometria de fluxoSystemic sclerosisMonocytesMonocyte subpopulationsFlow cytometryPathogenesisFlow cytometry evaluation of CD14/CD16 monocyte subpopulations in systemic sclerosis patients : a cross sectional controlled studyinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001130271.pdf.txt001130271.pdf.txtExtracted Texttext/plain43490http://www.lume.ufrgs.br/bitstream/10183/229342/2/001130271.pdf.txt920be9fff297a7a2eaa15f82608ec157MD52ORIGINAL001130271.pdfTexto completo (inglês)application/pdf1618535http://www.lume.ufrgs.br/bitstream/10183/229342/1/001130271.pdfeb98e011a4dae1a4ab72d8ce60f6bfe6MD5110183/2293422023-05-24 03:25:44.006492oai:www.lume.ufrgs.br:10183/229342Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-05-24T06:25:44Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Flow cytometry evaluation of CD14/CD16 monocyte subpopulations in systemic sclerosis patients : a cross sectional controlled study |
title |
Flow cytometry evaluation of CD14/CD16 monocyte subpopulations in systemic sclerosis patients : a cross sectional controlled study |
spellingShingle |
Flow cytometry evaluation of CD14/CD16 monocyte subpopulations in systemic sclerosis patients : a cross sectional controlled study Schneider, Laiana Escleroderma sistêmico Monocitos Citometria de fluxo Systemic sclerosis Monocytes Monocyte subpopulations Flow cytometry Pathogenesis |
title_short |
Flow cytometry evaluation of CD14/CD16 monocyte subpopulations in systemic sclerosis patients : a cross sectional controlled study |
title_full |
Flow cytometry evaluation of CD14/CD16 monocyte subpopulations in systemic sclerosis patients : a cross sectional controlled study |
title_fullStr |
Flow cytometry evaluation of CD14/CD16 monocyte subpopulations in systemic sclerosis patients : a cross sectional controlled study |
title_full_unstemmed |
Flow cytometry evaluation of CD14/CD16 monocyte subpopulations in systemic sclerosis patients : a cross sectional controlled study |
title_sort |
Flow cytometry evaluation of CD14/CD16 monocyte subpopulations in systemic sclerosis patients : a cross sectional controlled study |
author |
Schneider, Laiana |
author_facet |
Schneider, Laiana Marcondes, Natália Aydos Hax, Vanessa Moreira, Isadora Flesch da Silva Ueda, Carolina Yuka Piovesan, Rafaella Romeiro Xavier, Ricardo Machado Chakr, Rafael Mendonça da Silva |
author_role |
author |
author2 |
Marcondes, Natália Aydos Hax, Vanessa Moreira, Isadora Flesch da Silva Ueda, Carolina Yuka Piovesan, Rafaella Romeiro Xavier, Ricardo Machado Chakr, Rafael Mendonça da Silva |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Schneider, Laiana Marcondes, Natália Aydos Hax, Vanessa Moreira, Isadora Flesch da Silva Ueda, Carolina Yuka Piovesan, Rafaella Romeiro Xavier, Ricardo Machado Chakr, Rafael Mendonça da Silva |
dc.subject.por.fl_str_mv |
Escleroderma sistêmico Monocitos Citometria de fluxo |
topic |
Escleroderma sistêmico Monocitos Citometria de fluxo Systemic sclerosis Monocytes Monocyte subpopulations Flow cytometry Pathogenesis |
dc.subject.eng.fl_str_mv |
Systemic sclerosis Monocytes Monocyte subpopulations Flow cytometry Pathogenesis |
description |
Background: Systemic sclerosis (SSc) is a chronic autoimmune disease characterized by vasculopathy and fibrosis, which can be subclassified into diffuse cutaneous (dSSc) and limited cutaneous (lSSc) subtypes. Previous studies suggest that an increase in monocytes can be a hallmark of various inflammatory diseases, including SSc. Our aim was to evaluate circulating blood monocyte subpopulations (classical, intermediate and non-classical) of SSc patients and their possible association with disease manifestations. Methods: Fifty consecutive patients fulfilling the 2013 ACR/EULAR classification criteria for SSc were included in a cross-sectional study. Monocyte subpopulations were identified based on their expression of CD64, CD14 and CD16, evaluated by flow cytometry, and were correlated with the clinical characteristics of the patients; furthermore, the expression of HLA-DR, CD163, CD169 and CD206 in the monocytes was studied. Thirty-eight age- and sex-matched healthy individuals were recruited as a control group. Results: SSc patients had an increased number of circulating peripheral blood monocytes with an activated phenotypic profile compared to healthy subjects. Absolute counts of CD16+ (intermediary and non-classical) monocyte subpopulations were higher in SSc patients. There was no association between monocyte subpopulations and the clinical manifestations evaluated. Conclusion: We identified higher counts of all monocyte subpopulations in SSc patients compared to the control group. There was no association between monocyte subpopulations and major fibrotic manifestations. CD169 was shown to be more representative in dSSc, being a promising marker for differentiating disease subtypes. |
publishDate |
2021 |
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2021-09-01T04:27:21Z |
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2021 |
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http://hdl.handle.net/10183/229342 |
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2523-3106 |
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001130271 |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Advances in rheumatology. São Paulo. Vol. 61 (2021), 27, 11 p. |
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openAccess |
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