Comparative quantification of umbilical cord blood CD34+ and CD34+ bright cells using the procount tm-bd and ishage protocols
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/21197 |
Resumo: | The total number of CD34+ cells is the most relevant clinical parameter when selecting human umbilical cord blood (HUCB) for transplantation. The objective of the present study was to compare the two most commonly used CD34+ cell quantification methods (ISHAGE protocol and ProCount™ - BD) and analyze the CD34+ bright cells whose 7-amino actinomycin D (7AAD) analysis suggests are apoptotic or dead cells. Twenty-six HUCB samples obtained at the Placental Blood Program of New York Blood Center were evaluated. The absolute numbers of CD34+ cells evaluated by the ISHAGE (with exclusion of 7AAD+ cells) and ProCount™ (with exclusion of CD34+ bright cells) were determined. Using the ISHAGE protocol we found 35.6 ± 19.4 CD34+ cells/μL and with the ProCount™ method we found 36.6 ± 23.2 CD34+ cells/μL. With the ProCount™ method, CD34+ bright cell counts were 9.3 ± 8.2 cells/μL. CD34+ bright and regular cells were individually analyzed by the ISHAGE protocol. Only about 1.8% of the bright CD34+ cells are alive, whereas a small part (19.0%) is undergoing apoptosis and most of them (79.2%) are dead cells. Our study showed that the two methods produced similar results and that 7AAD is important to exclude CD34 bright cells. These results will be of value to assist in the correct counting of CD34+ cells and to choose the best HUCB unit for transplantation, i.e., the unit with the greatest number of potentially viable stem cells for the reconstitution of bone marrow. This increases the likelihood of success of the transplant and, therefore, the survival of the patient. |
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Pranke, Patricia Helena LucasHendrikx, JanAlespeite, GabrielNardi, Nance BeyerRubinstein, PabloVisser, Jan2010-04-24T04:15:41Z20060100-879Xhttp://hdl.handle.net/10183/21197000544257The total number of CD34+ cells is the most relevant clinical parameter when selecting human umbilical cord blood (HUCB) for transplantation. The objective of the present study was to compare the two most commonly used CD34+ cell quantification methods (ISHAGE protocol and ProCount™ - BD) and analyze the CD34+ bright cells whose 7-amino actinomycin D (7AAD) analysis suggests are apoptotic or dead cells. Twenty-six HUCB samples obtained at the Placental Blood Program of New York Blood Center were evaluated. The absolute numbers of CD34+ cells evaluated by the ISHAGE (with exclusion of 7AAD+ cells) and ProCount™ (with exclusion of CD34+ bright cells) were determined. Using the ISHAGE protocol we found 35.6 ± 19.4 CD34+ cells/μL and with the ProCount™ method we found 36.6 ± 23.2 CD34+ cells/μL. With the ProCount™ method, CD34+ bright cell counts were 9.3 ± 8.2 cells/μL. CD34+ bright and regular cells were individually analyzed by the ISHAGE protocol. Only about 1.8% of the bright CD34+ cells are alive, whereas a small part (19.0%) is undergoing apoptosis and most of them (79.2%) are dead cells. Our study showed that the two methods produced similar results and that 7AAD is important to exclude CD34 bright cells. These results will be of value to assist in the correct counting of CD34+ cells and to choose the best HUCB unit for transplantation, i.e., the unit with the greatest number of potentially viable stem cells for the reconstitution of bone marrow. This increases the likelihood of success of the transplant and, therefore, the survival of the patient.application/pdfengBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 39, n. 7 (Jul. 2006), p. 901-906Células-tronco hematopoéticasCordão umbilicalHematopoietic stem cellHuman umbilical cord bloodCD34+ISHAGEProCountComparative quantification of umbilical cord blood CD34+ and CD34+ bright cells using the procount tm-bd and ishage protocolsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000544257.pdf000544257.pdfTexto completo (inglês)application/pdf48174http://www.lume.ufrgs.br/bitstream/10183/21197/1/000544257.pdfeeab5f6c8ada120f701c9526bd3abf26MD51TEXT000544257.pdf.txt000544257.pdf.txtExtracted Texttext/plain24623http://www.lume.ufrgs.br/bitstream/10183/21197/2/000544257.pdf.txte519a206b03df8027ec8c2f75a1f4bc0MD52THUMBNAIL000544257.pdf.jpg000544257.pdf.jpgGenerated Thumbnailimage/jpeg1613http://www.lume.ufrgs.br/bitstream/10183/21197/3/000544257.pdf.jpg63327a07f19e21f5cb2a21b054a2cff9MD5310183/211972019-09-04 02:35:05.286255oai:www.lume.ufrgs.br:10183/21197Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-09-04T05:35:05Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Comparative quantification of umbilical cord blood CD34+ and CD34+ bright cells using the procount tm-bd and ishage protocols |
title |
Comparative quantification of umbilical cord blood CD34+ and CD34+ bright cells using the procount tm-bd and ishage protocols |
spellingShingle |
Comparative quantification of umbilical cord blood CD34+ and CD34+ bright cells using the procount tm-bd and ishage protocols Pranke, Patricia Helena Lucas Células-tronco hematopoéticas Cordão umbilical Hematopoietic stem cell Human umbilical cord blood CD34+ ISHAGE ProCount |
title_short |
Comparative quantification of umbilical cord blood CD34+ and CD34+ bright cells using the procount tm-bd and ishage protocols |
title_full |
Comparative quantification of umbilical cord blood CD34+ and CD34+ bright cells using the procount tm-bd and ishage protocols |
title_fullStr |
Comparative quantification of umbilical cord blood CD34+ and CD34+ bright cells using the procount tm-bd and ishage protocols |
title_full_unstemmed |
Comparative quantification of umbilical cord blood CD34+ and CD34+ bright cells using the procount tm-bd and ishage protocols |
title_sort |
Comparative quantification of umbilical cord blood CD34+ and CD34+ bright cells using the procount tm-bd and ishage protocols |
author |
Pranke, Patricia Helena Lucas |
author_facet |
Pranke, Patricia Helena Lucas Hendrikx, Jan Alespeite, Gabriel Nardi, Nance Beyer Rubinstein, Pablo Visser, Jan |
author_role |
author |
author2 |
Hendrikx, Jan Alespeite, Gabriel Nardi, Nance Beyer Rubinstein, Pablo Visser, Jan |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Pranke, Patricia Helena Lucas Hendrikx, Jan Alespeite, Gabriel Nardi, Nance Beyer Rubinstein, Pablo Visser, Jan |
dc.subject.por.fl_str_mv |
Células-tronco hematopoéticas Cordão umbilical |
topic |
Células-tronco hematopoéticas Cordão umbilical Hematopoietic stem cell Human umbilical cord blood CD34+ ISHAGE ProCount |
dc.subject.eng.fl_str_mv |
Hematopoietic stem cell Human umbilical cord blood CD34+ ISHAGE ProCount |
description |
The total number of CD34+ cells is the most relevant clinical parameter when selecting human umbilical cord blood (HUCB) for transplantation. The objective of the present study was to compare the two most commonly used CD34+ cell quantification methods (ISHAGE protocol and ProCount™ - BD) and analyze the CD34+ bright cells whose 7-amino actinomycin D (7AAD) analysis suggests are apoptotic or dead cells. Twenty-six HUCB samples obtained at the Placental Blood Program of New York Blood Center were evaluated. The absolute numbers of CD34+ cells evaluated by the ISHAGE (with exclusion of 7AAD+ cells) and ProCount™ (with exclusion of CD34+ bright cells) were determined. Using the ISHAGE protocol we found 35.6 ± 19.4 CD34+ cells/μL and with the ProCount™ method we found 36.6 ± 23.2 CD34+ cells/μL. With the ProCount™ method, CD34+ bright cell counts were 9.3 ± 8.2 cells/μL. CD34+ bright and regular cells were individually analyzed by the ISHAGE protocol. Only about 1.8% of the bright CD34+ cells are alive, whereas a small part (19.0%) is undergoing apoptosis and most of them (79.2%) are dead cells. Our study showed that the two methods produced similar results and that 7AAD is important to exclude CD34 bright cells. These results will be of value to assist in the correct counting of CD34+ cells and to choose the best HUCB unit for transplantation, i.e., the unit with the greatest number of potentially viable stem cells for the reconstitution of bone marrow. This increases the likelihood of success of the transplant and, therefore, the survival of the patient. |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006 |
dc.date.accessioned.fl_str_mv |
2010-04-24T04:15:41Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/21197 |
dc.identifier.issn.pt_BR.fl_str_mv |
0100-879X |
dc.identifier.nrb.pt_BR.fl_str_mv |
000544257 |
identifier_str_mv |
0100-879X 000544257 |
url |
http://hdl.handle.net/10183/21197 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 39, n. 7 (Jul. 2006), p. 901-906 |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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