Evaluation of total protein, peroxidase, and nutrients measured by pXRF for the determination of tissue rejuvenation / reinvigoration of Eucalyptus microcorys
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/265808 |
Resumo: | Ontogenetic aging of tissues and the gradual decrease of adventitious rooting are known challenges for the clonal propagation of woody species, hampering clonal forestry programs. This study examined possible signatures of tissue rejuvenation/reinvigoration in different propagated materials of Eucalyptus microcorys by analyzing the total protein profile, peroxidase activity, macro-and micronutrient contents, and adventitious rooting of mini cuttings. The analyses were performed on E. microcorys shoots which were successfully obtained by seminal and grafting propagation, micropropagation with epicormic shoots, and indirect organogenesis. Among four mature trees used in the propagation, tissues from the one with the best propagation results were investigated for signs of tissue rejuvenation and/or reinvigoration. Five individuals from each technique were randomly selected and transferred to a semi-hydroponic “channel” system. After four weeks in the seedbed, the total protein, peroxidase activity, nutrient content and rooting of the minicuttings were evaluated. SDS-PAGE enabled the differentiation of leaf samples obtained by grafting from the other propagation techniques, as revealed by two distinct bands. Materials obtained by micropropagation with epicormic shoots showed the highest peroxidase activity, while those obtained by seminal propagation and from the selected mature tree showed the lowest peroxidase activity. A portable X-ray fluorescence spectroscope (pXRF) identified adequate nutrient content in most of the nutrients tested in materials obtained by seminal and grafting propagation, and by indirect organogenesis. The analysis of adventitious rooting showed that the highest rooting percentage was observed in mini cuttings from seminal propagation (75%) followed by indirect organogenesis (35%). Based on principal component analysis, it was concluded that rooting of mini cuttings from both seminal propagation and indirect organogenesis organogenesis was associated with phosphorous, sulphur, and potassium contents, which suggests a higher level of tissue rejuvenation/reinvigoration in these propagated plants. Further studies are recommended to search for other methods that present similarities with the responses to adventitious rooting in forest species and thus optimize the rescue and propagation of plants with distinct ontogenetic stages. |
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Faria, Júlio Cézar TannureRibeiro-Kumara, CaiusDelarmelina, William MacedoNamorato, Filipe AiuraMomolli, Dione RicherJosé, Anderson CleitonKonzen, Enéas RicardoCarvalho, Dulcinéia deBrondani, Gilvano Ebling2023-10-11T03:35:30Z20231993-0607http://hdl.handle.net/10183/265808001176625Ontogenetic aging of tissues and the gradual decrease of adventitious rooting are known challenges for the clonal propagation of woody species, hampering clonal forestry programs. This study examined possible signatures of tissue rejuvenation/reinvigoration in different propagated materials of Eucalyptus microcorys by analyzing the total protein profile, peroxidase activity, macro-and micronutrient contents, and adventitious rooting of mini cuttings. The analyses were performed on E. microcorys shoots which were successfully obtained by seminal and grafting propagation, micropropagation with epicormic shoots, and indirect organogenesis. Among four mature trees used in the propagation, tissues from the one with the best propagation results were investigated for signs of tissue rejuvenation and/or reinvigoration. Five individuals from each technique were randomly selected and transferred to a semi-hydroponic “channel” system. After four weeks in the seedbed, the total protein, peroxidase activity, nutrient content and rooting of the minicuttings were evaluated. SDS-PAGE enabled the differentiation of leaf samples obtained by grafting from the other propagation techniques, as revealed by two distinct bands. Materials obtained by micropropagation with epicormic shoots showed the highest peroxidase activity, while those obtained by seminal propagation and from the selected mature tree showed the lowest peroxidase activity. A portable X-ray fluorescence spectroscope (pXRF) identified adequate nutrient content in most of the nutrients tested in materials obtained by seminal and grafting propagation, and by indirect organogenesis. The analysis of adventitious rooting showed that the highest rooting percentage was observed in mini cuttings from seminal propagation (75%) followed by indirect organogenesis (35%). Based on principal component analysis, it was concluded that rooting of mini cuttings from both seminal propagation and indirect organogenesis organogenesis was associated with phosphorous, sulphur, and potassium contents, which suggests a higher level of tissue rejuvenation/reinvigoration in these propagated plants. Further studies are recommended to search for other methods that present similarities with the responses to adventitious rooting in forest species and thus optimize the rescue and propagation of plants with distinct ontogenetic stages.application/pdfengJournalof Forestry Research. Swizerland. Vol. 34, no. 5 (Oct. 2023), p. 1563-1576EucaliptoPropagação vegetativaOntogenéticaIluminação artificialGenética vegetalFisiologia vegetal : LuzNutricao vegetalAdventitious rootingJuvenilityReinvigorationRejuvenationOntogenetic ageEvaluation of total protein, peroxidase, and nutrients measured by pXRF for the determination of tissue rejuvenation / reinvigoration of Eucalyptus microcorysEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001176625.pdf.txt001176625.pdf.txtExtracted Texttext/plain65856http://www.lume.ufrgs.br/bitstream/10183/265808/2/001176625.pdf.txtf8ed40e375ebf9068bc5a41c23adebdeMD52ORIGINAL001176625.pdfTexto completo (inglês)application/pdf1974814http://www.lume.ufrgs.br/bitstream/10183/265808/1/001176625.pdfdeef15d8a66283247fe72900bb94c7d8MD5110183/2658082023-10-12 03:32:39.986138oai:www.lume.ufrgs.br:10183/265808Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-10-12T06:32:39Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Evaluation of total protein, peroxidase, and nutrients measured by pXRF for the determination of tissue rejuvenation / reinvigoration of Eucalyptus microcorys |
title |
Evaluation of total protein, peroxidase, and nutrients measured by pXRF for the determination of tissue rejuvenation / reinvigoration of Eucalyptus microcorys |
spellingShingle |
Evaluation of total protein, peroxidase, and nutrients measured by pXRF for the determination of tissue rejuvenation / reinvigoration of Eucalyptus microcorys Faria, Júlio Cézar Tannure Eucalipto Propagação vegetativa Ontogenética Iluminação artificial Genética vegetal Fisiologia vegetal : Luz Nutricao vegetal Adventitious rooting Juvenility Reinvigoration Rejuvenation Ontogenetic age |
title_short |
Evaluation of total protein, peroxidase, and nutrients measured by pXRF for the determination of tissue rejuvenation / reinvigoration of Eucalyptus microcorys |
title_full |
Evaluation of total protein, peroxidase, and nutrients measured by pXRF for the determination of tissue rejuvenation / reinvigoration of Eucalyptus microcorys |
title_fullStr |
Evaluation of total protein, peroxidase, and nutrients measured by pXRF for the determination of tissue rejuvenation / reinvigoration of Eucalyptus microcorys |
title_full_unstemmed |
Evaluation of total protein, peroxidase, and nutrients measured by pXRF for the determination of tissue rejuvenation / reinvigoration of Eucalyptus microcorys |
title_sort |
Evaluation of total protein, peroxidase, and nutrients measured by pXRF for the determination of tissue rejuvenation / reinvigoration of Eucalyptus microcorys |
author |
Faria, Júlio Cézar Tannure |
author_facet |
Faria, Júlio Cézar Tannure Ribeiro-Kumara, Caius Delarmelina, William Macedo Namorato, Filipe Aiura Momolli, Dione Richer José, Anderson Cleiton Konzen, Enéas Ricardo Carvalho, Dulcinéia de Brondani, Gilvano Ebling |
author_role |
author |
author2 |
Ribeiro-Kumara, Caius Delarmelina, William Macedo Namorato, Filipe Aiura Momolli, Dione Richer José, Anderson Cleiton Konzen, Enéas Ricardo Carvalho, Dulcinéia de Brondani, Gilvano Ebling |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Faria, Júlio Cézar Tannure Ribeiro-Kumara, Caius Delarmelina, William Macedo Namorato, Filipe Aiura Momolli, Dione Richer José, Anderson Cleiton Konzen, Enéas Ricardo Carvalho, Dulcinéia de Brondani, Gilvano Ebling |
dc.subject.por.fl_str_mv |
Eucalipto Propagação vegetativa Ontogenética Iluminação artificial Genética vegetal Fisiologia vegetal : Luz Nutricao vegetal |
topic |
Eucalipto Propagação vegetativa Ontogenética Iluminação artificial Genética vegetal Fisiologia vegetal : Luz Nutricao vegetal Adventitious rooting Juvenility Reinvigoration Rejuvenation Ontogenetic age |
dc.subject.eng.fl_str_mv |
Adventitious rooting Juvenility Reinvigoration Rejuvenation Ontogenetic age |
description |
Ontogenetic aging of tissues and the gradual decrease of adventitious rooting are known challenges for the clonal propagation of woody species, hampering clonal forestry programs. This study examined possible signatures of tissue rejuvenation/reinvigoration in different propagated materials of Eucalyptus microcorys by analyzing the total protein profile, peroxidase activity, macro-and micronutrient contents, and adventitious rooting of mini cuttings. The analyses were performed on E. microcorys shoots which were successfully obtained by seminal and grafting propagation, micropropagation with epicormic shoots, and indirect organogenesis. Among four mature trees used in the propagation, tissues from the one with the best propagation results were investigated for signs of tissue rejuvenation and/or reinvigoration. Five individuals from each technique were randomly selected and transferred to a semi-hydroponic “channel” system. After four weeks in the seedbed, the total protein, peroxidase activity, nutrient content and rooting of the minicuttings were evaluated. SDS-PAGE enabled the differentiation of leaf samples obtained by grafting from the other propagation techniques, as revealed by two distinct bands. Materials obtained by micropropagation with epicormic shoots showed the highest peroxidase activity, while those obtained by seminal propagation and from the selected mature tree showed the lowest peroxidase activity. A portable X-ray fluorescence spectroscope (pXRF) identified adequate nutrient content in most of the nutrients tested in materials obtained by seminal and grafting propagation, and by indirect organogenesis. The analysis of adventitious rooting showed that the highest rooting percentage was observed in mini cuttings from seminal propagation (75%) followed by indirect organogenesis (35%). Based on principal component analysis, it was concluded that rooting of mini cuttings from both seminal propagation and indirect organogenesis organogenesis was associated with phosphorous, sulphur, and potassium contents, which suggests a higher level of tissue rejuvenation/reinvigoration in these propagated plants. Further studies are recommended to search for other methods that present similarities with the responses to adventitious rooting in forest species and thus optimize the rescue and propagation of plants with distinct ontogenetic stages. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-10-11T03:35:30Z |
dc.date.issued.fl_str_mv |
2023 |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/265808 |
dc.identifier.issn.pt_BR.fl_str_mv |
1993-0607 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001176625 |
identifier_str_mv |
1993-0607 001176625 |
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http://hdl.handle.net/10183/265808 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Journalof Forestry Research. Swizerland. Vol. 34, no. 5 (Oct. 2023), p. 1563-1576 |
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info:eu-repo/semantics/openAccess |
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