Treatment of limbal melanocytoma with full excision and a fresh homologous corneoscleral graft in a dog

Detalhes bibliográficos
Autor(a) principal: Pigatto, João Antonio Tadeu
Data de Publicação: 2020
Outros Autores: Albuquerque, Luciane, Pigatto, Anita Marchionatti, Silva, Alessandra Fernandez da, Torikachvili, Marcela, Vargas, Eduarda Valim Borges de, Driemeier, David
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/211827
Resumo: Background: Limbal melanoma has been diagnosed in dogs and due to progression may cause vision loss and eyeball removal. Definitive diagnosis is made through histopathological examination. Therapeutic options include full thickness resection and repair by homologous corneal tissue, synthetic graft material, and enucleation. In this report, we describe a case of limbal melanocitoma in a dog that has been treated successfully with fresh homologous corneoscleral graft. Case: A 5-year-old female Labrador was referred to the Ophthalmology Veterinary Section of the Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil, with a history of a pigmented mass located on the left eye. Ophthalmic examination revealed a pigmented mass located at the left temporal limbus with corneal involvement. Surgical excision followed by reconstruction using fresh homologous corneoscleral was recommended. The patient was premedicated with acepromazine (0.05 mg/kg, IM) and meperidine (20 mg/kg, IM). Anaesthesia was induced with propofol (10 mg/kg, IV) and maintained with isoflurane. Atracurium (0.2 mg/kg, IV) was administered to maintain a central eye position. The mass and a free margin were removed by full-thickness corneoscleral resection. A corneoscleral graft was harvested from a dog that had been euthanised for reasons unrelated to this study and sutured with 9-0 polyglactin 910 using a simple interrupted pattern. The mass was immediately fixed in 10% neutral buffered formalin and submitted for histological sectioning and routine staining. Based on the histopathological analysis it was confirmed limbal melanocytoma. Postoperative treatment consisted of topical administration of 0.3% flurbiprofen every 6 h for 15 days, and a combination of topical ciprofloxacin/ dexamethasone eye drops every 6 h for 30 days. Systemic carprofen (4 mg/kg per day, VO) was prescribed for 10 days. Topical tropicamide was used twice daily for 1 week. Cyclosporin 0.2% eye drops were applied twice daily for 2 months. Examination of the left eye two months after surgery revealed decreased corneal vascularization, and the results of pupillary light response and vision testing were normal. The values of intraocular pressure remained normal in all postoperative evaluations. The patient was followed for 36 months postoperatively, during which time there was no recurrence. Discussion: Limbal melanomas are the most common ocular melanomas in dogs. Most of them develop slowly and are located closely to the superior limbus. The tumors tend to grow more rapidly in younger dogs and more slowly in older dogs. Labrador Retrievers and German shepherds appear to be affected more frequently than other breeds. The present case involved a 5-year-old Labrador breed. The clinical presentation included a pigmented limbal mass extending into the adjacent cornea, sclera and conjunctival tissue. A tissue biopsy is necessary to confirm the diagnosis. In the present case, the diagnosis of limbal melanocytoma was based on clinical signs and confirmed by histopathological examination. The choice of treatment is influenced by the tumour size and location, availability of equipment and materials, clinician expertise and the cost of treatment. Some techniques for removing the mass and repairing the resultant corneoscleral defect has been described. In the present case, due to the location and size of the mass and the absence of intraocular invasion, surgical removal including a margin of normal tissue was performed. With this surgical procedure, the intention was to preserve the eyeball and maintain vision. In this case, surgical excision of a limbal melanocytoma combined with homologous corneoscleral graft was effective for repairing a full-thickness corneoscleral defect and preserving ocular function.
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spelling Pigatto, João Antonio TadeuAlbuquerque, LucianePigatto, Anita MarchionattiSilva, Alessandra Fernandez daTorikachvili, MarcelaVargas, Eduarda Valim Borges deDriemeier, David2020-07-11T03:53:23Z20201678-0345http://hdl.handle.net/10183/211827001115661Background: Limbal melanoma has been diagnosed in dogs and due to progression may cause vision loss and eyeball removal. Definitive diagnosis is made through histopathological examination. Therapeutic options include full thickness resection and repair by homologous corneal tissue, synthetic graft material, and enucleation. In this report, we describe a case of limbal melanocitoma in a dog that has been treated successfully with fresh homologous corneoscleral graft. Case: A 5-year-old female Labrador was referred to the Ophthalmology Veterinary Section of the Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil, with a history of a pigmented mass located on the left eye. Ophthalmic examination revealed a pigmented mass located at the left temporal limbus with corneal involvement. Surgical excision followed by reconstruction using fresh homologous corneoscleral was recommended. The patient was premedicated with acepromazine (0.05 mg/kg, IM) and meperidine (20 mg/kg, IM). Anaesthesia was induced with propofol (10 mg/kg, IV) and maintained with isoflurane. Atracurium (0.2 mg/kg, IV) was administered to maintain a central eye position. The mass and a free margin were removed by full-thickness corneoscleral resection. A corneoscleral graft was harvested from a dog that had been euthanised for reasons unrelated to this study and sutured with 9-0 polyglactin 910 using a simple interrupted pattern. The mass was immediately fixed in 10% neutral buffered formalin and submitted for histological sectioning and routine staining. Based on the histopathological analysis it was confirmed limbal melanocytoma. Postoperative treatment consisted of topical administration of 0.3% flurbiprofen every 6 h for 15 days, and a combination of topical ciprofloxacin/ dexamethasone eye drops every 6 h for 30 days. Systemic carprofen (4 mg/kg per day, VO) was prescribed for 10 days. Topical tropicamide was used twice daily for 1 week. Cyclosporin 0.2% eye drops were applied twice daily for 2 months. Examination of the left eye two months after surgery revealed decreased corneal vascularization, and the results of pupillary light response and vision testing were normal. The values of intraocular pressure remained normal in all postoperative evaluations. The patient was followed for 36 months postoperatively, during which time there was no recurrence. Discussion: Limbal melanomas are the most common ocular melanomas in dogs. Most of them develop slowly and are located closely to the superior limbus. The tumors tend to grow more rapidly in younger dogs and more slowly in older dogs. Labrador Retrievers and German shepherds appear to be affected more frequently than other breeds. The present case involved a 5-year-old Labrador breed. The clinical presentation included a pigmented limbal mass extending into the adjacent cornea, sclera and conjunctival tissue. A tissue biopsy is necessary to confirm the diagnosis. In the present case, the diagnosis of limbal melanocytoma was based on clinical signs and confirmed by histopathological examination. The choice of treatment is influenced by the tumour size and location, availability of equipment and materials, clinician expertise and the cost of treatment. Some techniques for removing the mass and repairing the resultant corneoscleral defect has been described. In the present case, due to the location and size of the mass and the absence of intraocular invasion, surgical removal including a margin of normal tissue was performed. With this surgical procedure, the intention was to preserve the eyeball and maintain vision. In this case, surgical excision of a limbal melanocytoma combined with homologous corneoscleral graft was effective for repairing a full-thickness corneoscleral defect and preserving ocular function.application/pdfengActa scientiae veterinariae. Porto Alegre, RS. Vol. 48, supl. 1 (2020), Pub. 504, 5 p.NeoplasiasLimbo da córneaTransplante de córneaCãesCanineFresh corneoscleral graftMelanocytic neoplasiaTreatment of limbal melanocytoma with full excision and a fresh homologous corneoscleral graft in a doginfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001115661.pdf.txt001115661.pdf.txtExtracted Texttext/plain21413http://www.lume.ufrgs.br/bitstream/10183/211827/2/001115661.pdf.txt6e0fcb2a59767f7e3ff9e5661f80a6e9MD52ORIGINAL001115661.pdfTexto completo (inglês)application/pdf938337http://www.lume.ufrgs.br/bitstream/10183/211827/1/001115661.pdf3b2fc858740c5588db9ebfd49c4d0027MD5110183/2118272020-07-12 03:42:11.793587oai:www.lume.ufrgs.br:10183/211827Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2020-07-12T06:42:11Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Treatment of limbal melanocytoma with full excision and a fresh homologous corneoscleral graft in a dog
title Treatment of limbal melanocytoma with full excision and a fresh homologous corneoscleral graft in a dog
spellingShingle Treatment of limbal melanocytoma with full excision and a fresh homologous corneoscleral graft in a dog
Pigatto, João Antonio Tadeu
Neoplasias
Limbo da córnea
Transplante de córnea
Cães
Canine
Fresh corneoscleral graft
Melanocytic neoplasia
title_short Treatment of limbal melanocytoma with full excision and a fresh homologous corneoscleral graft in a dog
title_full Treatment of limbal melanocytoma with full excision and a fresh homologous corneoscleral graft in a dog
title_fullStr Treatment of limbal melanocytoma with full excision and a fresh homologous corneoscleral graft in a dog
title_full_unstemmed Treatment of limbal melanocytoma with full excision and a fresh homologous corneoscleral graft in a dog
title_sort Treatment of limbal melanocytoma with full excision and a fresh homologous corneoscleral graft in a dog
author Pigatto, João Antonio Tadeu
author_facet Pigatto, João Antonio Tadeu
Albuquerque, Luciane
Pigatto, Anita Marchionatti
Silva, Alessandra Fernandez da
Torikachvili, Marcela
Vargas, Eduarda Valim Borges de
Driemeier, David
author_role author
author2 Albuquerque, Luciane
Pigatto, Anita Marchionatti
Silva, Alessandra Fernandez da
Torikachvili, Marcela
Vargas, Eduarda Valim Borges de
Driemeier, David
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pigatto, João Antonio Tadeu
Albuquerque, Luciane
Pigatto, Anita Marchionatti
Silva, Alessandra Fernandez da
Torikachvili, Marcela
Vargas, Eduarda Valim Borges de
Driemeier, David
dc.subject.por.fl_str_mv Neoplasias
Limbo da córnea
Transplante de córnea
Cães
topic Neoplasias
Limbo da córnea
Transplante de córnea
Cães
Canine
Fresh corneoscleral graft
Melanocytic neoplasia
dc.subject.eng.fl_str_mv Canine
Fresh corneoscleral graft
Melanocytic neoplasia
description Background: Limbal melanoma has been diagnosed in dogs and due to progression may cause vision loss and eyeball removal. Definitive diagnosis is made through histopathological examination. Therapeutic options include full thickness resection and repair by homologous corneal tissue, synthetic graft material, and enucleation. In this report, we describe a case of limbal melanocitoma in a dog that has been treated successfully with fresh homologous corneoscleral graft. Case: A 5-year-old female Labrador was referred to the Ophthalmology Veterinary Section of the Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil, with a history of a pigmented mass located on the left eye. Ophthalmic examination revealed a pigmented mass located at the left temporal limbus with corneal involvement. Surgical excision followed by reconstruction using fresh homologous corneoscleral was recommended. The patient was premedicated with acepromazine (0.05 mg/kg, IM) and meperidine (20 mg/kg, IM). Anaesthesia was induced with propofol (10 mg/kg, IV) and maintained with isoflurane. Atracurium (0.2 mg/kg, IV) was administered to maintain a central eye position. The mass and a free margin were removed by full-thickness corneoscleral resection. A corneoscleral graft was harvested from a dog that had been euthanised for reasons unrelated to this study and sutured with 9-0 polyglactin 910 using a simple interrupted pattern. The mass was immediately fixed in 10% neutral buffered formalin and submitted for histological sectioning and routine staining. Based on the histopathological analysis it was confirmed limbal melanocytoma. Postoperative treatment consisted of topical administration of 0.3% flurbiprofen every 6 h for 15 days, and a combination of topical ciprofloxacin/ dexamethasone eye drops every 6 h for 30 days. Systemic carprofen (4 mg/kg per day, VO) was prescribed for 10 days. Topical tropicamide was used twice daily for 1 week. Cyclosporin 0.2% eye drops were applied twice daily for 2 months. Examination of the left eye two months after surgery revealed decreased corneal vascularization, and the results of pupillary light response and vision testing were normal. The values of intraocular pressure remained normal in all postoperative evaluations. The patient was followed for 36 months postoperatively, during which time there was no recurrence. Discussion: Limbal melanomas are the most common ocular melanomas in dogs. Most of them develop slowly and are located closely to the superior limbus. The tumors tend to grow more rapidly in younger dogs and more slowly in older dogs. Labrador Retrievers and German shepherds appear to be affected more frequently than other breeds. The present case involved a 5-year-old Labrador breed. The clinical presentation included a pigmented limbal mass extending into the adjacent cornea, sclera and conjunctival tissue. A tissue biopsy is necessary to confirm the diagnosis. In the present case, the diagnosis of limbal melanocytoma was based on clinical signs and confirmed by histopathological examination. The choice of treatment is influenced by the tumour size and location, availability of equipment and materials, clinician expertise and the cost of treatment. Some techniques for removing the mass and repairing the resultant corneoscleral defect has been described. In the present case, due to the location and size of the mass and the absence of intraocular invasion, surgical removal including a margin of normal tissue was performed. With this surgical procedure, the intention was to preserve the eyeball and maintain vision. In this case, surgical excision of a limbal melanocytoma combined with homologous corneoscleral graft was effective for repairing a full-thickness corneoscleral defect and preserving ocular function.
publishDate 2020
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dc.relation.ispartof.pt_BR.fl_str_mv Acta scientiae veterinariae. Porto Alegre, RS. Vol. 48, supl. 1 (2020), Pub. 504, 5 p.
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