Production and evaluation of albino mutants of Sprorothrix schenckii
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/19934 |
Resumo: | Melanin is an important factor intrinsically related to the virulence of the Sporothrix schenckii. Mutant albino conidia are usually used as inoculants for the evaluation of the properties of melanin in experimental models for the study of sporotrichosis. The goals of this study were to analyze the presence of melanin in S. schenckii conidia, and to produce and evaluate mutant albino forms of S. Schenckii conidia. Four distinct isolates were cultured in PDA, supplemented or not with triciclazole, and in YEPD. A portion of the samples was exposed to 300 ergs/mm2 of UV radiation to generate mutants albino forms. Following the incubation, cultures were analyzed, CFU were determined, and fragments were removed for the ultrastructural analysis, to be cultured once more in YEPD e BHI. Albino colonies were exposed to visible light; all S. schenckii isolates produced melanin, and survival and viability were 66% and 96% for albino conidia. Melanin granules were visible in the cell wall of pigmented conidia, but not in albino conidia. All conidia reverted to both the pigmented and the yeast forms, and albino colonies photoreactivated. All isolates analyzed in this study generated DHN melanin. Moreover, despite the exposure to UV radiation (300 ergs/mm2) being lethal to a significant portion of the colonies, the viability and conversions to the pigmented and yeast forms in surviving colonies did not appear to be affected. Also, albino colonies photoreactivated upon exposure to visible light. |
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Nobre, Márcia de OliveiraAntunes, Tatiana de ÁvilaMeireles, Mário Carlos AraújoFerreiro, Laerte2010-04-16T09:12:17Z20041678-0345http://hdl.handle.net/10183/19934000469603Melanin is an important factor intrinsically related to the virulence of the Sporothrix schenckii. Mutant albino conidia are usually used as inoculants for the evaluation of the properties of melanin in experimental models for the study of sporotrichosis. The goals of this study were to analyze the presence of melanin in S. schenckii conidia, and to produce and evaluate mutant albino forms of S. Schenckii conidia. Four distinct isolates were cultured in PDA, supplemented or not with triciclazole, and in YEPD. A portion of the samples was exposed to 300 ergs/mm2 of UV radiation to generate mutants albino forms. Following the incubation, cultures were analyzed, CFU were determined, and fragments were removed for the ultrastructural analysis, to be cultured once more in YEPD e BHI. Albino colonies were exposed to visible light; all S. schenckii isolates produced melanin, and survival and viability were 66% and 96% for albino conidia. Melanin granules were visible in the cell wall of pigmented conidia, but not in albino conidia. All conidia reverted to both the pigmented and the yeast forms, and albino colonies photoreactivated. All isolates analyzed in this study generated DHN melanin. Moreover, despite the exposure to UV radiation (300 ergs/mm2) being lethal to a significant portion of the colonies, the viability and conversions to the pigmented and yeast forms in surviving colonies did not appear to be affected. Also, albino colonies photoreactivated upon exposure to visible light.application/pdfengActa scientiae veterinariae. Porto Alegre, RS. Vol. 32, n. 2 (2004), p. 119-123Sporothrix schenckiiConídios mutantes albinosSporothrix schenckiiAlbino mutantsUV lightSurvivalViabilityProduction and evaluation of albino mutants of Sprorothrix schenckiiProdução e avaliação de mutantes albinos de Sporothrix schenckii info:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000469603.pdf000469603.pdfTexto completo (inglês)application/pdf279814http://www.lume.ufrgs.br/bitstream/10183/19934/1/000469603.pdfd01d39eeefaa00c5e0a2380ae1c99113MD51TEXT000469603.pdf.txt000469603.pdf.txtExtracted Texttext/plain18893http://www.lume.ufrgs.br/bitstream/10183/19934/2/000469603.pdf.txt84648f60267b643d3ca7c0ad665119f8MD52THUMBNAIL000469603.pdf.jpg000469603.pdf.jpgGenerated Thumbnailimage/jpeg1717http://www.lume.ufrgs.br/bitstream/10183/19934/3/000469603.pdf.jpg362a4a04b3ad6712a9520384ba8f53d1MD5310183/199342021-06-13 04:30:13.477194oai:www.lume.ufrgs.br:10183/19934Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-06-13T07:30:13Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Production and evaluation of albino mutants of Sprorothrix schenckii |
dc.title.alternative.pt.fl_str_mv |
Produção e avaliação de mutantes albinos de Sporothrix schenckii |
title |
Production and evaluation of albino mutants of Sprorothrix schenckii |
spellingShingle |
Production and evaluation of albino mutants of Sprorothrix schenckii Nobre, Márcia de Oliveira Sporothrix schenckii Conídios mutantes albinos Sporothrix schenckii Albino mutants UV light Survival Viability |
title_short |
Production and evaluation of albino mutants of Sprorothrix schenckii |
title_full |
Production and evaluation of albino mutants of Sprorothrix schenckii |
title_fullStr |
Production and evaluation of albino mutants of Sprorothrix schenckii |
title_full_unstemmed |
Production and evaluation of albino mutants of Sprorothrix schenckii |
title_sort |
Production and evaluation of albino mutants of Sprorothrix schenckii |
author |
Nobre, Márcia de Oliveira |
author_facet |
Nobre, Márcia de Oliveira Antunes, Tatiana de Ávila Meireles, Mário Carlos Araújo Ferreiro, Laerte |
author_role |
author |
author2 |
Antunes, Tatiana de Ávila Meireles, Mário Carlos Araújo Ferreiro, Laerte |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Nobre, Márcia de Oliveira Antunes, Tatiana de Ávila Meireles, Mário Carlos Araújo Ferreiro, Laerte |
dc.subject.por.fl_str_mv |
Sporothrix schenckii Conídios mutantes albinos |
topic |
Sporothrix schenckii Conídios mutantes albinos Sporothrix schenckii Albino mutants UV light Survival Viability |
dc.subject.eng.fl_str_mv |
Sporothrix schenckii Albino mutants UV light Survival Viability |
description |
Melanin is an important factor intrinsically related to the virulence of the Sporothrix schenckii. Mutant albino conidia are usually used as inoculants for the evaluation of the properties of melanin in experimental models for the study of sporotrichosis. The goals of this study were to analyze the presence of melanin in S. schenckii conidia, and to produce and evaluate mutant albino forms of S. Schenckii conidia. Four distinct isolates were cultured in PDA, supplemented or not with triciclazole, and in YEPD. A portion of the samples was exposed to 300 ergs/mm2 of UV radiation to generate mutants albino forms. Following the incubation, cultures were analyzed, CFU were determined, and fragments were removed for the ultrastructural analysis, to be cultured once more in YEPD e BHI. Albino colonies were exposed to visible light; all S. schenckii isolates produced melanin, and survival and viability were 66% and 96% for albino conidia. Melanin granules were visible in the cell wall of pigmented conidia, but not in albino conidia. All conidia reverted to both the pigmented and the yeast forms, and albino colonies photoreactivated. All isolates analyzed in this study generated DHN melanin. Moreover, despite the exposure to UV radiation (300 ergs/mm2) being lethal to a significant portion of the colonies, the viability and conversions to the pigmented and yeast forms in surviving colonies did not appear to be affected. Also, albino colonies photoreactivated upon exposure to visible light. |
publishDate |
2004 |
dc.date.issued.fl_str_mv |
2004 |
dc.date.accessioned.fl_str_mv |
2010-04-16T09:12:17Z |
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http://hdl.handle.net/10183/19934 |
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1678-0345 |
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000469603 |
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http://hdl.handle.net/10183/19934 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Acta scientiae veterinariae. Porto Alegre, RS. Vol. 32, n. 2 (2004), p. 119-123 |
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