Liquid chromatography method to assay tretinoin in skin layers : validation and application in skin penetration/retention studies
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/231549 |
Resumo: | A liquid chromatography (LC) method for the quantification of tretinoin (TTN) in different matrices (adhesive tape, cotton and porcine skin layers, stratum corneum, viable epidermis, and dermis) was validated and applied in in vitro porcine skin penetration/retention studies. This study proposes, for the first time, a method for assaying TTN in separated porcine skin layers (stratum corneum, viable epidermis, and dermis). The skin studies were carried out using tape stripping and cutaneous retention techniques. The procedures for the extraction of TTN from dermatological formulations (creams and gels) and biological and non-biological matrices used with the tape stripping and retention techniques were also evaluated. The LC method consisted of a mobile phase composed of a mixture of methanol, water, and glacial acetic acid (85:15:1, v/v); a C18 column used as the stationary phase; a flow rate of 1.0 mL min 1; an injection volume of 100 μL; and TTN detection at 342 nm. The method was linear in the range of 0.05–15.00 μg mL 1 (r ¼ 0.9999), and it was precise and accurate. The limit of detection (LOD) and limit of quantification (LOQ) were 0.0165 μg mL 1 and 0.0495 μg mL 1, respectively. TTN was extracted from different matrices, showing good precision [relative standard deviation (RSD) of <5%] and accuracy (89.4–113.9%). This method was successfully applied in the evaluation of TTN skin retention/permeation from dermatological formulations (cream and gel). A higher penetration of TTN through the skin was achieved with the gel rather than the cream, showing the influence of the dosage form. Therefore, the developed method can easily be applied in porcine skin penetration/retention studies of dermatological formulations containing TTN, and it is able to discriminate the behaviours of the different formulations. |
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Oliveira, Dileusa deAndrade, Diego Fontana deOliveira, Edilene Gadelha deBeck, Ruy Carlos Ruver2021-11-04T04:24:36Z20202405-8440http://hdl.handle.net/10183/231549001131022A liquid chromatography (LC) method for the quantification of tretinoin (TTN) in different matrices (adhesive tape, cotton and porcine skin layers, stratum corneum, viable epidermis, and dermis) was validated and applied in in vitro porcine skin penetration/retention studies. This study proposes, for the first time, a method for assaying TTN in separated porcine skin layers (stratum corneum, viable epidermis, and dermis). The skin studies were carried out using tape stripping and cutaneous retention techniques. The procedures for the extraction of TTN from dermatological formulations (creams and gels) and biological and non-biological matrices used with the tape stripping and retention techniques were also evaluated. The LC method consisted of a mobile phase composed of a mixture of methanol, water, and glacial acetic acid (85:15:1, v/v); a C18 column used as the stationary phase; a flow rate of 1.0 mL min 1; an injection volume of 100 μL; and TTN detection at 342 nm. The method was linear in the range of 0.05–15.00 μg mL 1 (r ¼ 0.9999), and it was precise and accurate. The limit of detection (LOD) and limit of quantification (LOQ) were 0.0165 μg mL 1 and 0.0495 μg mL 1, respectively. TTN was extracted from different matrices, showing good precision [relative standard deviation (RSD) of <5%] and accuracy (89.4–113.9%). This method was successfully applied in the evaluation of TTN skin retention/permeation from dermatological formulations (cream and gel). A higher penetration of TTN through the skin was achieved with the gel rather than the cream, showing the influence of the dosage form. Therefore, the developed method can easily be applied in porcine skin penetration/retention studies of dermatological formulations containing TTN, and it is able to discriminate the behaviours of the different formulations.application/pdfengHeliyon. London. Vol. 6, n. 1 (Jan. 2020), e03098, 7 p.Cromatografia líquidaTretinoínaAbsorção cutâneaLiquid chromatographyTretinoinValidationSkin penetration and retentionAnalytical chemistryChromatographyPharmaceutical chemistryPharmaceutical scienceDrug deliveryLiquid chromatography method to assay tretinoin in skin layers : validation and application in skin penetration/retention studiesEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001131022.pdf.txt001131022.pdf.txtExtracted Texttext/plain40377http://www.lume.ufrgs.br/bitstream/10183/231549/2/001131022.pdf.txt2fda5baf1c93d911f2711f4c51cf38e2MD52ORIGINAL001131022.pdfTexto completo (inglês)application/pdf535555http://www.lume.ufrgs.br/bitstream/10183/231549/1/001131022.pdf29f97e561065b3f7e714ee92f166dfafMD5110183/2315492021-11-20 06:17:26.633025oai:www.lume.ufrgs.br:10183/231549Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-11-20T08:17:26Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Liquid chromatography method to assay tretinoin in skin layers : validation and application in skin penetration/retention studies |
title |
Liquid chromatography method to assay tretinoin in skin layers : validation and application in skin penetration/retention studies |
spellingShingle |
Liquid chromatography method to assay tretinoin in skin layers : validation and application in skin penetration/retention studies Oliveira, Dileusa de Cromatografia líquida Tretinoína Absorção cutânea Liquid chromatography Tretinoin Validation Skin penetration and retention Analytical chemistry Chromatography Pharmaceutical chemistry Pharmaceutical science Drug delivery |
title_short |
Liquid chromatography method to assay tretinoin in skin layers : validation and application in skin penetration/retention studies |
title_full |
Liquid chromatography method to assay tretinoin in skin layers : validation and application in skin penetration/retention studies |
title_fullStr |
Liquid chromatography method to assay tretinoin in skin layers : validation and application in skin penetration/retention studies |
title_full_unstemmed |
Liquid chromatography method to assay tretinoin in skin layers : validation and application in skin penetration/retention studies |
title_sort |
Liquid chromatography method to assay tretinoin in skin layers : validation and application in skin penetration/retention studies |
author |
Oliveira, Dileusa de |
author_facet |
Oliveira, Dileusa de Andrade, Diego Fontana de Oliveira, Edilene Gadelha de Beck, Ruy Carlos Ruver |
author_role |
author |
author2 |
Andrade, Diego Fontana de Oliveira, Edilene Gadelha de Beck, Ruy Carlos Ruver |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Dileusa de Andrade, Diego Fontana de Oliveira, Edilene Gadelha de Beck, Ruy Carlos Ruver |
dc.subject.por.fl_str_mv |
Cromatografia líquida Tretinoína Absorção cutânea |
topic |
Cromatografia líquida Tretinoína Absorção cutânea Liquid chromatography Tretinoin Validation Skin penetration and retention Analytical chemistry Chromatography Pharmaceutical chemistry Pharmaceutical science Drug delivery |
dc.subject.eng.fl_str_mv |
Liquid chromatography Tretinoin Validation Skin penetration and retention Analytical chemistry Chromatography Pharmaceutical chemistry Pharmaceutical science Drug delivery |
description |
A liquid chromatography (LC) method for the quantification of tretinoin (TTN) in different matrices (adhesive tape, cotton and porcine skin layers, stratum corneum, viable epidermis, and dermis) was validated and applied in in vitro porcine skin penetration/retention studies. This study proposes, for the first time, a method for assaying TTN in separated porcine skin layers (stratum corneum, viable epidermis, and dermis). The skin studies were carried out using tape stripping and cutaneous retention techniques. The procedures for the extraction of TTN from dermatological formulations (creams and gels) and biological and non-biological matrices used with the tape stripping and retention techniques were also evaluated. The LC method consisted of a mobile phase composed of a mixture of methanol, water, and glacial acetic acid (85:15:1, v/v); a C18 column used as the stationary phase; a flow rate of 1.0 mL min 1; an injection volume of 100 μL; and TTN detection at 342 nm. The method was linear in the range of 0.05–15.00 μg mL 1 (r ¼ 0.9999), and it was precise and accurate. The limit of detection (LOD) and limit of quantification (LOQ) were 0.0165 μg mL 1 and 0.0495 μg mL 1, respectively. TTN was extracted from different matrices, showing good precision [relative standard deviation (RSD) of <5%] and accuracy (89.4–113.9%). This method was successfully applied in the evaluation of TTN skin retention/permeation from dermatological formulations (cream and gel). A higher penetration of TTN through the skin was achieved with the gel rather than the cream, showing the influence of the dosage form. Therefore, the developed method can easily be applied in porcine skin penetration/retention studies of dermatological formulations containing TTN, and it is able to discriminate the behaviours of the different formulations. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020 |
dc.date.accessioned.fl_str_mv |
2021-11-04T04:24:36Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
format |
article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/231549 |
dc.identifier.issn.pt_BR.fl_str_mv |
2405-8440 |
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001131022 |
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2405-8440 001131022 |
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http://hdl.handle.net/10183/231549 |
dc.language.iso.fl_str_mv |
eng |
language |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Heliyon. London. Vol. 6, n. 1 (Jan. 2020), e03098, 7 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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