Determination of germ tube, phospholipase, and proteinase production by bloodstream isolates of Candida albicans
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/94965 |
Resumo: | Candida albicans is a commensal and opportunistic agent that causes infection in immunocompromised individuals. Several attributes contribute to the virulence and pathogenicity of this yeast, including the production of germ tubes (GTs) and extracellular hydrolytic enzymes, particularly phospholipase and proteinase. This study aimed to investigate GT production and phospholipase and proteinase activities in bloodstream isolates of C. albicans. Methods: One hundred fi fty-three C. albicans isolates were obtained from blood samples and analyzed for GT, phospholipase, and proteinase production. The assays were performed in duplicate in egg yolk medium containing bovine serum albumin and human serum. Results: Detectable amounts of proteinase were produced by 97% of the isolates, and 78% of the isolates produced phospholipase. GTs were produced by 95% of the isolates. A majority of the isolates exhibited low levels of phospholipase production and high levels of proteinase production. Conclusions: Bloodstream isolates of C. albicans produce virulence factors such as GT and hydrolytic enzymes that enable them to cause infection under favorable conditions. |
id |
UFRGS-2_d6cd99fa5597a8ee5b457b317639b887 |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/94965 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Mattei, Antonella SouzaAlves, Sydney HartzSevero, Cecília BittencourtGuazzelli, Luciana SilvaOliveira, Flávio de MattosSevero, Luiz Carlos2014-05-07T02:04:32Z20130037-8682http://hdl.handle.net/10183/94965000898157Candida albicans is a commensal and opportunistic agent that causes infection in immunocompromised individuals. Several attributes contribute to the virulence and pathogenicity of this yeast, including the production of germ tubes (GTs) and extracellular hydrolytic enzymes, particularly phospholipase and proteinase. This study aimed to investigate GT production and phospholipase and proteinase activities in bloodstream isolates of C. albicans. Methods: One hundred fi fty-three C. albicans isolates were obtained from blood samples and analyzed for GT, phospholipase, and proteinase production. The assays were performed in duplicate in egg yolk medium containing bovine serum albumin and human serum. Results: Detectable amounts of proteinase were produced by 97% of the isolates, and 78% of the isolates produced phospholipase. GTs were produced by 95% of the isolates. A majority of the isolates exhibited low levels of phospholipase production and high levels of proteinase production. Conclusions: Bloodstream isolates of C. albicans produce virulence factors such as GT and hydrolytic enzymes that enable them to cause infection under favorable conditions.application/pdfengRevista da Sociedade Brasileira de Medicina Tropical. Brasilia. Vol. 46, n. 3 (maio/jun. 2013), p. 340-342.VirulênciaFosfolipasesPeptídeo hidrolasesCandida albicansCandidemiaPhospholipaseCandida albicansCandidemiaProteinaseGerm tubeVirulenceDetermination of germ tube, phospholipase, and proteinase production by bloodstream isolates of Candida albicansEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000898157.pdf000898157.pdfTexto completo (inglês)application/pdf676841http://www.lume.ufrgs.br/bitstream/10183/94965/1/000898157.pdf0250315a482688b335839f1e6d476eb4MD51TEXT000898157.pdf.txt000898157.pdf.txtExtracted Texttext/plain15421http://www.lume.ufrgs.br/bitstream/10183/94965/2/000898157.pdf.txt03645ed688206693d2bed6e5ece8136aMD52THUMBNAIL000898157.pdf.jpg000898157.pdf.jpgGenerated Thumbnailimage/jpeg2332http://www.lume.ufrgs.br/bitstream/10183/94965/3/000898157.pdf.jpgf64090aecb3a47e0a6495d65d9c6e48eMD5310183/949652021-07-09 04:33:39.851239oai:www.lume.ufrgs.br:10183/94965Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-07-09T07:33:39Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Determination of germ tube, phospholipase, and proteinase production by bloodstream isolates of Candida albicans |
title |
Determination of germ tube, phospholipase, and proteinase production by bloodstream isolates of Candida albicans |
spellingShingle |
Determination of germ tube, phospholipase, and proteinase production by bloodstream isolates of Candida albicans Mattei, Antonella Souza Virulência Fosfolipases Peptídeo hidrolases Candida albicans Candidemia Phospholipase Candida albicans Candidemia Proteinase Germ tube Virulence |
title_short |
Determination of germ tube, phospholipase, and proteinase production by bloodstream isolates of Candida albicans |
title_full |
Determination of germ tube, phospholipase, and proteinase production by bloodstream isolates of Candida albicans |
title_fullStr |
Determination of germ tube, phospholipase, and proteinase production by bloodstream isolates of Candida albicans |
title_full_unstemmed |
Determination of germ tube, phospholipase, and proteinase production by bloodstream isolates of Candida albicans |
title_sort |
Determination of germ tube, phospholipase, and proteinase production by bloodstream isolates of Candida albicans |
author |
Mattei, Antonella Souza |
author_facet |
Mattei, Antonella Souza Alves, Sydney Hartz Severo, Cecília Bittencourt Guazzelli, Luciana Silva Oliveira, Flávio de Mattos Severo, Luiz Carlos |
author_role |
author |
author2 |
Alves, Sydney Hartz Severo, Cecília Bittencourt Guazzelli, Luciana Silva Oliveira, Flávio de Mattos Severo, Luiz Carlos |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Mattei, Antonella Souza Alves, Sydney Hartz Severo, Cecília Bittencourt Guazzelli, Luciana Silva Oliveira, Flávio de Mattos Severo, Luiz Carlos |
dc.subject.por.fl_str_mv |
Virulência Fosfolipases Peptídeo hidrolases Candida albicans Candidemia |
topic |
Virulência Fosfolipases Peptídeo hidrolases Candida albicans Candidemia Phospholipase Candida albicans Candidemia Proteinase Germ tube Virulence |
dc.subject.eng.fl_str_mv |
Phospholipase Candida albicans Candidemia Proteinase Germ tube Virulence |
description |
Candida albicans is a commensal and opportunistic agent that causes infection in immunocompromised individuals. Several attributes contribute to the virulence and pathogenicity of this yeast, including the production of germ tubes (GTs) and extracellular hydrolytic enzymes, particularly phospholipase and proteinase. This study aimed to investigate GT production and phospholipase and proteinase activities in bloodstream isolates of C. albicans. Methods: One hundred fi fty-three C. albicans isolates were obtained from blood samples and analyzed for GT, phospholipase, and proteinase production. The assays were performed in duplicate in egg yolk medium containing bovine serum albumin and human serum. Results: Detectable amounts of proteinase were produced by 97% of the isolates, and 78% of the isolates produced phospholipase. GTs were produced by 95% of the isolates. A majority of the isolates exhibited low levels of phospholipase production and high levels of proteinase production. Conclusions: Bloodstream isolates of C. albicans produce virulence factors such as GT and hydrolytic enzymes that enable them to cause infection under favorable conditions. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2014-05-07T02:04:32Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/94965 |
dc.identifier.issn.pt_BR.fl_str_mv |
0037-8682 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000898157 |
identifier_str_mv |
0037-8682 000898157 |
url |
http://hdl.handle.net/10183/94965 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical. Brasilia. Vol. 46, n. 3 (maio/jun. 2013), p. 340-342. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/94965/1/000898157.pdf http://www.lume.ufrgs.br/bitstream/10183/94965/2/000898157.pdf.txt http://www.lume.ufrgs.br/bitstream/10183/94965/3/000898157.pdf.jpg |
bitstream.checksum.fl_str_mv |
0250315a482688b335839f1e6d476eb4 03645ed688206693d2bed6e5ece8136a f64090aecb3a47e0a6495d65d9c6e48e |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
|
_version_ |
1815447541328642048 |