Isoflavone-aglycone fraction from Glycine max : a promising raw material for isoflavone-based pharmaceutical or nutraceutical products
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/230171 |
Resumo: | he present work was designed to obtain a fraction containing high concentration of isoflavone-aglycones from Glycine max (L.) Merr., Fabaceae, named isoflavone enriched-fraction, from a dry extractcontaining isoflavones-glycosides. A simple and low cost method was proposed: extraction of isoflavoneglycosides from the G. max dry extract with a proper solvent, hydrolysis of the glycosides, recovery ofthe aglycones, and purification of the fraction containing high isoflavone-aglycones concentration. Allthe extraction and purification parameters were optimized based on the isoflavones yields, which wereanalyzed by liquid chromatography and expressed as total isoflavone aglycones. The optimization of theprocess conditions was accomplished using the classical one-variable-at-a-time method. The identity andpurity of the isoflavones contained in this enriched fraction were determined by LC/UV/ESI/MS analysis,Fourier transformed-infrared spectroscopy, and1H and13C nuclear magnetic resonance spectroscopy.The physicochemical properties of the isoflavone enriched-fraction were evaluated by scanning elec-tron microscopy, X-ray diffraction and differential scanning calorimetry. The moisture content, particlesize, equilibrium solubility and thermal and photostability were also determined. The high isoflavone-aglycone content (daidzein, 489.35 mg/g; glycitein, 251.02 mg/g and genistein, 158.96 mg/g) as well asthe high purity obtained (90% of total isoflavones) make this fraction a promising novel raw material forthe production of isoflavone-aglycones based pharmaceuticals or functional foods. |
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Yatsu, Francini Kiyono JorgeBassani, Valquiria LinckKoester, Leticia Scherer2021-09-24T04:22:18Z20160102-695Xhttp://hdl.handle.net/10183/230171000989414he present work was designed to obtain a fraction containing high concentration of isoflavone-aglycones from Glycine max (L.) Merr., Fabaceae, named isoflavone enriched-fraction, from a dry extractcontaining isoflavones-glycosides. A simple and low cost method was proposed: extraction of isoflavoneglycosides from the G. max dry extract with a proper solvent, hydrolysis of the glycosides, recovery ofthe aglycones, and purification of the fraction containing high isoflavone-aglycones concentration. Allthe extraction and purification parameters were optimized based on the isoflavones yields, which wereanalyzed by liquid chromatography and expressed as total isoflavone aglycones. The optimization of theprocess conditions was accomplished using the classical one-variable-at-a-time method. The identity andpurity of the isoflavones contained in this enriched fraction were determined by LC/UV/ESI/MS analysis,Fourier transformed-infrared spectroscopy, and1H and13C nuclear magnetic resonance spectroscopy.The physicochemical properties of the isoflavone enriched-fraction were evaluated by scanning elec-tron microscopy, X-ray diffraction and differential scanning calorimetry. The moisture content, particlesize, equilibrium solubility and thermal and photostability were also determined. The high isoflavone-aglycone content (daidzein, 489.35 mg/g; glycitein, 251.02 mg/g and genistein, 158.96 mg/g) as well asthe high purity obtained (90% of total isoflavones) make this fraction a promising novel raw material forthe production of isoflavone-aglycones based pharmaceuticals or functional foods.application/pdfengRevista brasileira de farmacognosia. São Paulo: Elsevier, 2016. Vol. 26 (2016), p. 259-267FarmáciaIsoflavonasIsoflavone-aglycone fractionDaidzeinFlavonoidsSoyIsoflavone-aglycone fraction from Glycine max : a promising raw material for isoflavone-based pharmaceutical or nutraceutical productsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000989414.pdf.txt000989414.pdf.txtExtracted Texttext/plain45335http://www.lume.ufrgs.br/bitstream/10183/230171/2/000989414.pdf.txt118e37d45d1f86ad5bb35412cc3efa10MD52ORIGINAL000989414.pdfTexto completo (inglês)application/pdf1865492http://www.lume.ufrgs.br/bitstream/10183/230171/1/000989414.pdfbec189b8350594e3c8be1c7e4b109258MD5110183/2301712021-10-04 04:23:17.681209oai:www.lume.ufrgs.br:10183/230171Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-10-04T07:23:17Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Isoflavone-aglycone fraction from Glycine max : a promising raw material for isoflavone-based pharmaceutical or nutraceutical products |
title |
Isoflavone-aglycone fraction from Glycine max : a promising raw material for isoflavone-based pharmaceutical or nutraceutical products |
spellingShingle |
Isoflavone-aglycone fraction from Glycine max : a promising raw material for isoflavone-based pharmaceutical or nutraceutical products Yatsu, Francini Kiyono Jorge Farmácia Isoflavonas Isoflavone-aglycone fraction Daidzein Flavonoids Soy |
title_short |
Isoflavone-aglycone fraction from Glycine max : a promising raw material for isoflavone-based pharmaceutical or nutraceutical products |
title_full |
Isoflavone-aglycone fraction from Glycine max : a promising raw material for isoflavone-based pharmaceutical or nutraceutical products |
title_fullStr |
Isoflavone-aglycone fraction from Glycine max : a promising raw material for isoflavone-based pharmaceutical or nutraceutical products |
title_full_unstemmed |
Isoflavone-aglycone fraction from Glycine max : a promising raw material for isoflavone-based pharmaceutical or nutraceutical products |
title_sort |
Isoflavone-aglycone fraction from Glycine max : a promising raw material for isoflavone-based pharmaceutical or nutraceutical products |
author |
Yatsu, Francini Kiyono Jorge |
author_facet |
Yatsu, Francini Kiyono Jorge Bassani, Valquiria Linck Koester, Leticia Scherer |
author_role |
author |
author2 |
Bassani, Valquiria Linck Koester, Leticia Scherer |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Yatsu, Francini Kiyono Jorge Bassani, Valquiria Linck Koester, Leticia Scherer |
dc.subject.por.fl_str_mv |
Farmácia Isoflavonas |
topic |
Farmácia Isoflavonas Isoflavone-aglycone fraction Daidzein Flavonoids Soy |
dc.subject.eng.fl_str_mv |
Isoflavone-aglycone fraction Daidzein Flavonoids Soy |
description |
he present work was designed to obtain a fraction containing high concentration of isoflavone-aglycones from Glycine max (L.) Merr., Fabaceae, named isoflavone enriched-fraction, from a dry extractcontaining isoflavones-glycosides. A simple and low cost method was proposed: extraction of isoflavoneglycosides from the G. max dry extract with a proper solvent, hydrolysis of the glycosides, recovery ofthe aglycones, and purification of the fraction containing high isoflavone-aglycones concentration. Allthe extraction and purification parameters were optimized based on the isoflavones yields, which wereanalyzed by liquid chromatography and expressed as total isoflavone aglycones. The optimization of theprocess conditions was accomplished using the classical one-variable-at-a-time method. The identity andpurity of the isoflavones contained in this enriched fraction were determined by LC/UV/ESI/MS analysis,Fourier transformed-infrared spectroscopy, and1H and13C nuclear magnetic resonance spectroscopy.The physicochemical properties of the isoflavone enriched-fraction were evaluated by scanning elec-tron microscopy, X-ray diffraction and differential scanning calorimetry. The moisture content, particlesize, equilibrium solubility and thermal and photostability were also determined. The high isoflavone-aglycone content (daidzein, 489.35 mg/g; glycitein, 251.02 mg/g and genistein, 158.96 mg/g) as well asthe high purity obtained (90% of total isoflavones) make this fraction a promising novel raw material forthe production of isoflavone-aglycones based pharmaceuticals or functional foods. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2021-09-24T04:22:18Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/230171 |
dc.identifier.issn.pt_BR.fl_str_mv |
0102-695X |
dc.identifier.nrb.pt_BR.fl_str_mv |
000989414 |
identifier_str_mv |
0102-695X 000989414 |
url |
http://hdl.handle.net/10183/230171 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Revista brasileira de farmacognosia. São Paulo: Elsevier, 2016. Vol. 26 (2016), p. 259-267 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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