Isoflavone-aglycone fraction from Glycine max : a promising raw material for isoflavone-based pharmaceutical or nutraceutical products

Detalhes bibliográficos
Autor(a) principal: Yatsu, Francini Kiyono Jorge
Data de Publicação: 2016
Outros Autores: Bassani, Valquiria Linck, Koester, Leticia Scherer
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/230171
Resumo: he present work was designed to obtain a fraction containing high concentration of isoflavone-aglycones from Glycine max (L.) Merr., Fabaceae, named isoflavone enriched-fraction, from a dry extractcontaining isoflavones-glycosides. A simple and low cost method was proposed: extraction of isoflavoneglycosides from the G. max dry extract with a proper solvent, hydrolysis of the glycosides, recovery ofthe aglycones, and purification of the fraction containing high isoflavone-aglycones concentration. Allthe extraction and purification parameters were optimized based on the isoflavones yields, which wereanalyzed by liquid chromatography and expressed as total isoflavone aglycones. The optimization of theprocess conditions was accomplished using the classical one-variable-at-a-time method. The identity andpurity of the isoflavones contained in this enriched fraction were determined by LC/UV/ESI/MS analysis,Fourier transformed-infrared spectroscopy, and1H and13C nuclear magnetic resonance spectroscopy.The physicochemical properties of the isoflavone enriched-fraction were evaluated by scanning elec-tron microscopy, X-ray diffraction and differential scanning calorimetry. The moisture content, particlesize, equilibrium solubility and thermal and photostability were also determined. The high isoflavone-aglycone content (daidzein, 489.35 mg/g; glycitein, 251.02 mg/g and genistein, 158.96 mg/g) as well asthe high purity obtained (90% of total isoflavones) make this fraction a promising novel raw material forthe production of isoflavone-aglycones based pharmaceuticals or functional foods.
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spelling Yatsu, Francini Kiyono JorgeBassani, Valquiria LinckKoester, Leticia Scherer2021-09-24T04:22:18Z20160102-695Xhttp://hdl.handle.net/10183/230171000989414he present work was designed to obtain a fraction containing high concentration of isoflavone-aglycones from Glycine max (L.) Merr., Fabaceae, named isoflavone enriched-fraction, from a dry extractcontaining isoflavones-glycosides. A simple and low cost method was proposed: extraction of isoflavoneglycosides from the G. max dry extract with a proper solvent, hydrolysis of the glycosides, recovery ofthe aglycones, and purification of the fraction containing high isoflavone-aglycones concentration. Allthe extraction and purification parameters were optimized based on the isoflavones yields, which wereanalyzed by liquid chromatography and expressed as total isoflavone aglycones. The optimization of theprocess conditions was accomplished using the classical one-variable-at-a-time method. The identity andpurity of the isoflavones contained in this enriched fraction were determined by LC/UV/ESI/MS analysis,Fourier transformed-infrared spectroscopy, and1H and13C nuclear magnetic resonance spectroscopy.The physicochemical properties of the isoflavone enriched-fraction were evaluated by scanning elec-tron microscopy, X-ray diffraction and differential scanning calorimetry. The moisture content, particlesize, equilibrium solubility and thermal and photostability were also determined. The high isoflavone-aglycone content (daidzein, 489.35 mg/g; glycitein, 251.02 mg/g and genistein, 158.96 mg/g) as well asthe high purity obtained (90% of total isoflavones) make this fraction a promising novel raw material forthe production of isoflavone-aglycones based pharmaceuticals or functional foods.application/pdfengRevista brasileira de farmacognosia. São Paulo: Elsevier, 2016. Vol. 26 (2016), p. 259-267FarmáciaIsoflavonasIsoflavone-aglycone fractionDaidzeinFlavonoidsSoyIsoflavone-aglycone fraction from Glycine max : a promising raw material for isoflavone-based pharmaceutical or nutraceutical productsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000989414.pdf.txt000989414.pdf.txtExtracted Texttext/plain45335http://www.lume.ufrgs.br/bitstream/10183/230171/2/000989414.pdf.txt118e37d45d1f86ad5bb35412cc3efa10MD52ORIGINAL000989414.pdfTexto completo (inglês)application/pdf1865492http://www.lume.ufrgs.br/bitstream/10183/230171/1/000989414.pdfbec189b8350594e3c8be1c7e4b109258MD5110183/2301712021-10-04 04:23:17.681209oai:www.lume.ufrgs.br:10183/230171Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-10-04T07:23:17Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Isoflavone-aglycone fraction from Glycine max : a promising raw material for isoflavone-based pharmaceutical or nutraceutical products
title Isoflavone-aglycone fraction from Glycine max : a promising raw material for isoflavone-based pharmaceutical or nutraceutical products
spellingShingle Isoflavone-aglycone fraction from Glycine max : a promising raw material for isoflavone-based pharmaceutical or nutraceutical products
Yatsu, Francini Kiyono Jorge
Farmácia
Isoflavonas
Isoflavone-aglycone fraction
Daidzein
Flavonoids
Soy
title_short Isoflavone-aglycone fraction from Glycine max : a promising raw material for isoflavone-based pharmaceutical or nutraceutical products
title_full Isoflavone-aglycone fraction from Glycine max : a promising raw material for isoflavone-based pharmaceutical or nutraceutical products
title_fullStr Isoflavone-aglycone fraction from Glycine max : a promising raw material for isoflavone-based pharmaceutical or nutraceutical products
title_full_unstemmed Isoflavone-aglycone fraction from Glycine max : a promising raw material for isoflavone-based pharmaceutical or nutraceutical products
title_sort Isoflavone-aglycone fraction from Glycine max : a promising raw material for isoflavone-based pharmaceutical or nutraceutical products
author Yatsu, Francini Kiyono Jorge
author_facet Yatsu, Francini Kiyono Jorge
Bassani, Valquiria Linck
Koester, Leticia Scherer
author_role author
author2 Bassani, Valquiria Linck
Koester, Leticia Scherer
author2_role author
author
dc.contributor.author.fl_str_mv Yatsu, Francini Kiyono Jorge
Bassani, Valquiria Linck
Koester, Leticia Scherer
dc.subject.por.fl_str_mv Farmácia
Isoflavonas
topic Farmácia
Isoflavonas
Isoflavone-aglycone fraction
Daidzein
Flavonoids
Soy
dc.subject.eng.fl_str_mv Isoflavone-aglycone fraction
Daidzein
Flavonoids
Soy
description he present work was designed to obtain a fraction containing high concentration of isoflavone-aglycones from Glycine max (L.) Merr., Fabaceae, named isoflavone enriched-fraction, from a dry extractcontaining isoflavones-glycosides. A simple and low cost method was proposed: extraction of isoflavoneglycosides from the G. max dry extract with a proper solvent, hydrolysis of the glycosides, recovery ofthe aglycones, and purification of the fraction containing high isoflavone-aglycones concentration. Allthe extraction and purification parameters were optimized based on the isoflavones yields, which wereanalyzed by liquid chromatography and expressed as total isoflavone aglycones. The optimization of theprocess conditions was accomplished using the classical one-variable-at-a-time method. The identity andpurity of the isoflavones contained in this enriched fraction were determined by LC/UV/ESI/MS analysis,Fourier transformed-infrared spectroscopy, and1H and13C nuclear magnetic resonance spectroscopy.The physicochemical properties of the isoflavone enriched-fraction were evaluated by scanning elec-tron microscopy, X-ray diffraction and differential scanning calorimetry. The moisture content, particlesize, equilibrium solubility and thermal and photostability were also determined. The high isoflavone-aglycone content (daidzein, 489.35 mg/g; glycitein, 251.02 mg/g and genistein, 158.96 mg/g) as well asthe high purity obtained (90% of total isoflavones) make this fraction a promising novel raw material forthe production of isoflavone-aglycones based pharmaceuticals or functional foods.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2021-09-24T04:22:18Z
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dc.identifier.issn.pt_BR.fl_str_mv 0102-695X
dc.identifier.nrb.pt_BR.fl_str_mv 000989414
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000989414
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dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Revista brasileira de farmacognosia. São Paulo: Elsevier, 2016. Vol. 26 (2016), p. 259-267
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reponame_str Repositório Institucional da UFRGS
collection Repositório Institucional da UFRGS
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