Quantitative and qualitative evaluation of resin-dentin bond strengths : in vitro effects of surface treatment with metalloproteinase inhibitors
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/107002 |
Resumo: | AIM: The objective of this study was to evaluate the use of MMPs inhibitors (chlorhexidine and EDTA) in bond strength and quality of the hybrid layer of adhesive restorations in normal dentin using two ethanol-based total-etch adhesive systems. MATERIAL AND METHODS: Thirty-two extracted human molars were coronally sectioned and randomly divided into 8 groups (n=4), depending on the surface pre-treatment and adhesive system used. The total-etch adhesive systems Single Bond 2 (2-step) and Adper Scotchbond Multi-Purpose Plus (3-step) were used as follows: 1) according to manufacturer's instructions (etching with 37% phosphoric acid (H₃P0₄) for 15 s); 2) etching with H₃P0₄ for 15 s, followed by 2% chlorhexidine for 120 s; 3) etching with 0.1 M EDTA for 60 s; 4) etching with 0.1 M EDTA followed by 2% chlorhexidine for 120 s. Teeth were incrementally restored with composite resin (Filtek Z350XT). After water storage for 24 h, teeth were double-sectioned, yielding stick specimens of 1.0 mm² bonded area, and then subjected to microtensile bond strength (MTBS) test at 0.5 mm/min. Additional specimens were gold-sputtered to be analyzed under scanning electron microscopy (SEM). Data (in Mega Pascal) were subjected to Kruskal-Wallis and Dunn (p <0.05) tests. RESULTS: The etching protocol (37% H₃P0₄ or EDTA) interfered with hybrid layer formation, monomer penetration and the MTBS. Funnel shaped resin tags were observed when dentin was etched with 37% H₃P0₄. In these specimens, MTBS were also higher. EDTA conditioning produced thin hybrid layers and smaller MTBS, regardless the adhesive system used. Chlorhexidine application after conditioning resulted in no apparent differences between both evaluated techniques (37% H₃P0₄ or EDTA). CONCLUSION: The use of chlorhexidine as a MMP inhibitor does not alter immediate bond strength values and does not interfere with hybrid layer formation. |
id |
UFRGS-2_da3e7c2edcc28c6ff8972f814f785688 |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/107002 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Totti, MarceloSilva, Marcelo Goulart Rodrigues daFagundes, Laerte OhseFeldens, Thaís ThoméConceição, Ewerton NocchiCoelho de Souza, Fábio HerrmannErhardt, Maria Carolina Guilherme2014-11-15T02:16:00Z20142317-5907http://hdl.handle.net/10183/107002000931839AIM: The objective of this study was to evaluate the use of MMPs inhibitors (chlorhexidine and EDTA) in bond strength and quality of the hybrid layer of adhesive restorations in normal dentin using two ethanol-based total-etch adhesive systems. MATERIAL AND METHODS: Thirty-two extracted human molars were coronally sectioned and randomly divided into 8 groups (n=4), depending on the surface pre-treatment and adhesive system used. The total-etch adhesive systems Single Bond 2 (2-step) and Adper Scotchbond Multi-Purpose Plus (3-step) were used as follows: 1) according to manufacturer's instructions (etching with 37% phosphoric acid (H₃P0₄) for 15 s); 2) etching with H₃P0₄ for 15 s, followed by 2% chlorhexidine for 120 s; 3) etching with 0.1 M EDTA for 60 s; 4) etching with 0.1 M EDTA followed by 2% chlorhexidine for 120 s. Teeth were incrementally restored with composite resin (Filtek Z350XT). After water storage for 24 h, teeth were double-sectioned, yielding stick specimens of 1.0 mm² bonded area, and then subjected to microtensile bond strength (MTBS) test at 0.5 mm/min. Additional specimens were gold-sputtered to be analyzed under scanning electron microscopy (SEM). Data (in Mega Pascal) were subjected to Kruskal-Wallis and Dunn (p <0.05) tests. RESULTS: The etching protocol (37% H₃P0₄ or EDTA) interfered with hybrid layer formation, monomer penetration and the MTBS. Funnel shaped resin tags were observed when dentin was etched with 37% H₃P0₄. In these specimens, MTBS were also higher. EDTA conditioning produced thin hybrid layers and smaller MTBS, regardless the adhesive system used. Chlorhexidine application after conditioning resulted in no apparent differences between both evaluated techniques (37% H₃P0₄ or EDTA). CONCLUSION: The use of chlorhexidine as a MMP inhibitor does not alter immediate bond strength values and does not interfere with hybrid layer formation.application/pdfengJournal of Research in Dentistry. Tubarão, SC. Vol. 2, no. 3 (May/June 2014), p. 251-260Materiais odontológicosCimentos dentáriosClorexidinaDentin-Bonding agentsMetalloproteinasesChlorexidineEDTAQuantitative and qualitative evaluation of resin-dentin bond strengths : in vitro effects of surface treatment with metalloproteinase inhibitorsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000931839.pdf.txt000931839.pdf.txtExtracted Texttext/plain26256http://www.lume.ufrgs.br/bitstream/10183/107002/2/000931839.pdf.txt295815326ff748a733b883ea5d5c3bacMD52ORIGINAL000931839.pdfTexto completo (inglês)application/pdf1637257http://www.lume.ufrgs.br/bitstream/10183/107002/1/000931839.pdfbb5347e2b76832b03d14ecc258fafd40MD5110183/1070022022-03-26 05:11:29.730152oai:www.lume.ufrgs.br:10183/107002Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2022-03-26T08:11:29Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Quantitative and qualitative evaluation of resin-dentin bond strengths : in vitro effects of surface treatment with metalloproteinase inhibitors |
title |
Quantitative and qualitative evaluation of resin-dentin bond strengths : in vitro effects of surface treatment with metalloproteinase inhibitors |
spellingShingle |
Quantitative and qualitative evaluation of resin-dentin bond strengths : in vitro effects of surface treatment with metalloproteinase inhibitors Totti, Marcelo Materiais odontológicos Cimentos dentários Clorexidina Dentin-Bonding agents Metalloproteinases Chlorexidine EDTA |
title_short |
Quantitative and qualitative evaluation of resin-dentin bond strengths : in vitro effects of surface treatment with metalloproteinase inhibitors |
title_full |
Quantitative and qualitative evaluation of resin-dentin bond strengths : in vitro effects of surface treatment with metalloproteinase inhibitors |
title_fullStr |
Quantitative and qualitative evaluation of resin-dentin bond strengths : in vitro effects of surface treatment with metalloproteinase inhibitors |
title_full_unstemmed |
Quantitative and qualitative evaluation of resin-dentin bond strengths : in vitro effects of surface treatment with metalloproteinase inhibitors |
title_sort |
Quantitative and qualitative evaluation of resin-dentin bond strengths : in vitro effects of surface treatment with metalloproteinase inhibitors |
author |
Totti, Marcelo |
author_facet |
Totti, Marcelo Silva, Marcelo Goulart Rodrigues da Fagundes, Laerte Ohse Feldens, Thaís Thomé Conceição, Ewerton Nocchi Coelho de Souza, Fábio Herrmann Erhardt, Maria Carolina Guilherme |
author_role |
author |
author2 |
Silva, Marcelo Goulart Rodrigues da Fagundes, Laerte Ohse Feldens, Thaís Thomé Conceição, Ewerton Nocchi Coelho de Souza, Fábio Herrmann Erhardt, Maria Carolina Guilherme |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Totti, Marcelo Silva, Marcelo Goulart Rodrigues da Fagundes, Laerte Ohse Feldens, Thaís Thomé Conceição, Ewerton Nocchi Coelho de Souza, Fábio Herrmann Erhardt, Maria Carolina Guilherme |
dc.subject.por.fl_str_mv |
Materiais odontológicos Cimentos dentários Clorexidina |
topic |
Materiais odontológicos Cimentos dentários Clorexidina Dentin-Bonding agents Metalloproteinases Chlorexidine EDTA |
dc.subject.eng.fl_str_mv |
Dentin-Bonding agents Metalloproteinases Chlorexidine EDTA |
description |
AIM: The objective of this study was to evaluate the use of MMPs inhibitors (chlorhexidine and EDTA) in bond strength and quality of the hybrid layer of adhesive restorations in normal dentin using two ethanol-based total-etch adhesive systems. MATERIAL AND METHODS: Thirty-two extracted human molars were coronally sectioned and randomly divided into 8 groups (n=4), depending on the surface pre-treatment and adhesive system used. The total-etch adhesive systems Single Bond 2 (2-step) and Adper Scotchbond Multi-Purpose Plus (3-step) were used as follows: 1) according to manufacturer's instructions (etching with 37% phosphoric acid (H₃P0₄) for 15 s); 2) etching with H₃P0₄ for 15 s, followed by 2% chlorhexidine for 120 s; 3) etching with 0.1 M EDTA for 60 s; 4) etching with 0.1 M EDTA followed by 2% chlorhexidine for 120 s. Teeth were incrementally restored with composite resin (Filtek Z350XT). After water storage for 24 h, teeth were double-sectioned, yielding stick specimens of 1.0 mm² bonded area, and then subjected to microtensile bond strength (MTBS) test at 0.5 mm/min. Additional specimens were gold-sputtered to be analyzed under scanning electron microscopy (SEM). Data (in Mega Pascal) were subjected to Kruskal-Wallis and Dunn (p <0.05) tests. RESULTS: The etching protocol (37% H₃P0₄ or EDTA) interfered with hybrid layer formation, monomer penetration and the MTBS. Funnel shaped resin tags were observed when dentin was etched with 37% H₃P0₄. In these specimens, MTBS were also higher. EDTA conditioning produced thin hybrid layers and smaller MTBS, regardless the adhesive system used. Chlorhexidine application after conditioning resulted in no apparent differences between both evaluated techniques (37% H₃P0₄ or EDTA). CONCLUSION: The use of chlorhexidine as a MMP inhibitor does not alter immediate bond strength values and does not interfere with hybrid layer formation. |
publishDate |
2014 |
dc.date.accessioned.fl_str_mv |
2014-11-15T02:16:00Z |
dc.date.issued.fl_str_mv |
2014 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/107002 |
dc.identifier.issn.pt_BR.fl_str_mv |
2317-5907 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000931839 |
identifier_str_mv |
2317-5907 000931839 |
url |
http://hdl.handle.net/10183/107002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Journal of Research in Dentistry. Tubarão, SC. Vol. 2, no. 3 (May/June 2014), p. 251-260 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/107002/2/000931839.pdf.txt http://www.lume.ufrgs.br/bitstream/10183/107002/1/000931839.pdf |
bitstream.checksum.fl_str_mv |
295815326ff748a733b883ea5d5c3bac bb5347e2b76832b03d14ecc258fafd40 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
|
_version_ |
1801224856091492352 |