Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice (Oryza sativa L.) ionome
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/273871 |
Resumo: | NAC transcription factors are plant-specific proteins involved in many processes during the plant life cycle and responses to biotic and abiotic stresses. Previous studies have shown that stress-induced OsNAC5 from rice (Oryza sativa L.) is up-regulated by senescence and might be involved in control of iron (Fe) and zinc (Zn) concentrations in rice seeds. Aiming a better understanding of the role of OsNAC5 in rice plants, we investigated a mutant line carrying a T-DNA insertion in the promoter of OsNAC5, which resulted in enhanced expression of the transcription factor. Plants with OsNAC5 enhanced expression were shorter at the seedling stage and had reduced yield at maturity. In addition, we evaluated the expression level of OsNAC6, which is co-expressed with OsNAC5, and found that enhanced expression of OsNAC5 leads to increased expression of OsNAC6, suggesting that OsNAC5 might regulate OsNAC6 expression. Ionomic analysis of leaves and seeds from the OsNAC5 enhanced expression line revealed lower Fe and Zn concentrations in leaves and higher Fe concentrations in seeds than in WT plants, further suggesting that OsNAC5 may be involved in regulating the ionome in rice plants. Our work shows that fine-tuning of transcription factors is key when aiming at crop improvement. |
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Wairich, AndrieleVitali, ArianeAdamski, Janete MarizaLopes, Karina LetíciaDuarte, Guilherme LeitãoPonte, Lucas RoaniCosta, Henrique KellerMenguer, Paloma KoprovskiSantos, Rinaldo Pires dosFett, Janette PalmaSperotto, Raul AntonioRicachenevsky, Felipe Klein2024-03-19T05:06:23Z20231415-4757http://hdl.handle.net/10183/273871001172389NAC transcription factors are plant-specific proteins involved in many processes during the plant life cycle and responses to biotic and abiotic stresses. Previous studies have shown that stress-induced OsNAC5 from rice (Oryza sativa L.) is up-regulated by senescence and might be involved in control of iron (Fe) and zinc (Zn) concentrations in rice seeds. Aiming a better understanding of the role of OsNAC5 in rice plants, we investigated a mutant line carrying a T-DNA insertion in the promoter of OsNAC5, which resulted in enhanced expression of the transcription factor. Plants with OsNAC5 enhanced expression were shorter at the seedling stage and had reduced yield at maturity. In addition, we evaluated the expression level of OsNAC6, which is co-expressed with OsNAC5, and found that enhanced expression of OsNAC5 leads to increased expression of OsNAC6, suggesting that OsNAC5 might regulate OsNAC6 expression. Ionomic analysis of leaves and seeds from the OsNAC5 enhanced expression line revealed lower Fe and Zn concentrations in leaves and higher Fe concentrations in seeds than in WT plants, further suggesting that OsNAC5 may be involved in regulating the ionome in rice plants. Our work shows that fine-tuning of transcription factors is key when aiming at crop improvement.application/pdfengGenetics and molecular biology. Ribeirão Preto, SP. Vol. 46, no. 1 suppl. 1 (2023), e20220190, 12 p.Oryza sativaFerroZincoEstresseEnhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice (Oryza sativa L.) ionomeinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001172389.pdf.txt001172389.pdf.txtExtracted Texttext/plain63383http://www.lume.ufrgs.br/bitstream/10183/273871/2/001172389.pdf.txta07e4558a9b9e01c99316966a7bee066MD52ORIGINAL001172389.pdfTexto completo (inglês)application/pdf2493999http://www.lume.ufrgs.br/bitstream/10183/273871/1/001172389.pdf9c652f724f831ca1523c4024fc257691MD5110183/2738712024-03-20 04:51:10.442133oai:www.lume.ufrgs.br:10183/273871Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-03-20T07:51:10Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice (Oryza sativa L.) ionome |
title |
Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice (Oryza sativa L.) ionome |
spellingShingle |
Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice (Oryza sativa L.) ionome Wairich, Andriele Oryza sativa Ferro Zinco Estresse |
title_short |
Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice (Oryza sativa L.) ionome |
title_full |
Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice (Oryza sativa L.) ionome |
title_fullStr |
Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice (Oryza sativa L.) ionome |
title_full_unstemmed |
Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice (Oryza sativa L.) ionome |
title_sort |
Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice (Oryza sativa L.) ionome |
author |
Wairich, Andriele |
author_facet |
Wairich, Andriele Vitali, Ariane Adamski, Janete Mariza Lopes, Karina Letícia Duarte, Guilherme Leitão Ponte, Lucas Roani Costa, Henrique Keller Menguer, Paloma Koprovski Santos, Rinaldo Pires dos Fett, Janette Palma Sperotto, Raul Antonio Ricachenevsky, Felipe Klein |
author_role |
author |
author2 |
Vitali, Ariane Adamski, Janete Mariza Lopes, Karina Letícia Duarte, Guilherme Leitão Ponte, Lucas Roani Costa, Henrique Keller Menguer, Paloma Koprovski Santos, Rinaldo Pires dos Fett, Janette Palma Sperotto, Raul Antonio Ricachenevsky, Felipe Klein |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Wairich, Andriele Vitali, Ariane Adamski, Janete Mariza Lopes, Karina Letícia Duarte, Guilherme Leitão Ponte, Lucas Roani Costa, Henrique Keller Menguer, Paloma Koprovski Santos, Rinaldo Pires dos Fett, Janette Palma Sperotto, Raul Antonio Ricachenevsky, Felipe Klein |
dc.subject.por.fl_str_mv |
Oryza sativa Ferro Zinco Estresse |
topic |
Oryza sativa Ferro Zinco Estresse |
description |
NAC transcription factors are plant-specific proteins involved in many processes during the plant life cycle and responses to biotic and abiotic stresses. Previous studies have shown that stress-induced OsNAC5 from rice (Oryza sativa L.) is up-regulated by senescence and might be involved in control of iron (Fe) and zinc (Zn) concentrations in rice seeds. Aiming a better understanding of the role of OsNAC5 in rice plants, we investigated a mutant line carrying a T-DNA insertion in the promoter of OsNAC5, which resulted in enhanced expression of the transcription factor. Plants with OsNAC5 enhanced expression were shorter at the seedling stage and had reduced yield at maturity. In addition, we evaluated the expression level of OsNAC6, which is co-expressed with OsNAC5, and found that enhanced expression of OsNAC5 leads to increased expression of OsNAC6, suggesting that OsNAC5 might regulate OsNAC6 expression. Ionomic analysis of leaves and seeds from the OsNAC5 enhanced expression line revealed lower Fe and Zn concentrations in leaves and higher Fe concentrations in seeds than in WT plants, further suggesting that OsNAC5 may be involved in regulating the ionome in rice plants. Our work shows that fine-tuning of transcription factors is key when aiming at crop improvement. |
publishDate |
2023 |
dc.date.issued.fl_str_mv |
2023 |
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2024-03-19T05:06:23Z |
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1415-4757 |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Genetics and molecular biology. Ribeirão Preto, SP. Vol. 46, no. 1 suppl. 1 (2023), e20220190, 12 p. |
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