Virulence factors and antimicrobial resistance of escherichia coli isolated from urinary tract of swine in southern of Brazil
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/20838 |
Resumo: | The present study determined the molecular and resistance patterns of E. coli isolates from urinary tract of swine in Southern of Brazil. Molecular characterization of urinary vesicle samples was performed by PCR detection of virulence factors from ETEC, STEC and UPEC. From a total of 82 E. coli isolates, 34 (38.63%) harbored one or more virulence factors. The frequency of virulence factors genes detected by PCR were: pap (10.97%), hlyA (10.97%), iha (9.75%), lt (8.53%), sta (7.31%) sfa (6.09%), f4 (4.87%), f5 (4.87%), stb (4.87%), f6 (1.21%) and f41 (1.21%). Isolates were resistant to penicillin (95.12%), lincomycin (93.9%), erythromycin (92.68%), tetracycline (90.24%), amoxicillin (82.92%), ampicillin (74.39%), josamycin (79.26%), norfloxacin (58.53%), enrofloxacin (57.31%), gentamicin (39.02%), neomycin (37.8%), apramycin (30.48%), colistine (30.48%) and cefalexin (6.09%). A number of 32 (39.02%) E. coli isolates harbored plasmids. |
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Costa, Mateus Matiuzzi daDrescher, GuilhermeMaboni, FrancieleWeber, Shana de SoutoBotton, SoniaVainstein, Marilene HenningSchrank, Irene SilveiraVargas, Agueda Castagna de2010-04-16T09:16:51Z20081517-8382http://hdl.handle.net/10183/20838000719330The present study determined the molecular and resistance patterns of E. coli isolates from urinary tract of swine in Southern of Brazil. Molecular characterization of urinary vesicle samples was performed by PCR detection of virulence factors from ETEC, STEC and UPEC. From a total of 82 E. coli isolates, 34 (38.63%) harbored one or more virulence factors. The frequency of virulence factors genes detected by PCR were: pap (10.97%), hlyA (10.97%), iha (9.75%), lt (8.53%), sta (7.31%) sfa (6.09%), f4 (4.87%), f5 (4.87%), stb (4.87%), f6 (1.21%) and f41 (1.21%). Isolates were resistant to penicillin (95.12%), lincomycin (93.9%), erythromycin (92.68%), tetracycline (90.24%), amoxicillin (82.92%), ampicillin (74.39%), josamycin (79.26%), norfloxacin (58.53%), enrofloxacin (57.31%), gentamicin (39.02%), neomycin (37.8%), apramycin (30.48%), colistine (30.48%) and cefalexin (6.09%). A number of 32 (39.02%) E. coli isolates harbored plasmids.application/pdfengBrazilian journal of microbiology. São Paulo. Vol. 39, no. 4 (Dec. 2008), p. 741-743Escherichia coliFatores de virulênciaResistência antimicrobianaE. coliPlasmidsVirulence factorsAntimicrobial resistanceSwineVirulence factors and antimicrobial resistance of escherichia coli isolated from urinary tract of swine in southern of Brazilinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000719330.pdf000719330.pdfTexto completo (inglês)application/pdf199344http://www.lume.ufrgs.br/bitstream/10183/20838/1/000719330.pdf282124839a25beb22088d6052688b1b7MD51TEXT000719330.pdf.txt000719330.pdf.txtExtracted Texttext/plain15573http://www.lume.ufrgs.br/bitstream/10183/20838/2/000719330.pdf.txtbd6d2dda2f0ddcac1d2a45003b0df688MD52THUMBNAIL000719330.pdf.jpg000719330.pdf.jpgGenerated Thumbnailimage/jpeg2162http://www.lume.ufrgs.br/bitstream/10183/20838/3/000719330.pdf.jpg2939c99d252b0fc685ee13315be71656MD5310183/208382019-01-11 04:10:37.537049oai:www.lume.ufrgs.br:10183/20838Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-01-11T06:10:37Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Virulence factors and antimicrobial resistance of escherichia coli isolated from urinary tract of swine in southern of Brazil |
title |
Virulence factors and antimicrobial resistance of escherichia coli isolated from urinary tract of swine in southern of Brazil |
spellingShingle |
Virulence factors and antimicrobial resistance of escherichia coli isolated from urinary tract of swine in southern of Brazil Costa, Mateus Matiuzzi da Escherichia coli Fatores de virulência Resistência antimicrobiana E. coli Plasmids Virulence factors Antimicrobial resistance Swine |
title_short |
Virulence factors and antimicrobial resistance of escherichia coli isolated from urinary tract of swine in southern of Brazil |
title_full |
Virulence factors and antimicrobial resistance of escherichia coli isolated from urinary tract of swine in southern of Brazil |
title_fullStr |
Virulence factors and antimicrobial resistance of escherichia coli isolated from urinary tract of swine in southern of Brazil |
title_full_unstemmed |
Virulence factors and antimicrobial resistance of escherichia coli isolated from urinary tract of swine in southern of Brazil |
title_sort |
Virulence factors and antimicrobial resistance of escherichia coli isolated from urinary tract of swine in southern of Brazil |
author |
Costa, Mateus Matiuzzi da |
author_facet |
Costa, Mateus Matiuzzi da Drescher, Guilherme Maboni, Franciele Weber, Shana de Souto Botton, Sonia Vainstein, Marilene Henning Schrank, Irene Silveira Vargas, Agueda Castagna de |
author_role |
author |
author2 |
Drescher, Guilherme Maboni, Franciele Weber, Shana de Souto Botton, Sonia Vainstein, Marilene Henning Schrank, Irene Silveira Vargas, Agueda Castagna de |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Costa, Mateus Matiuzzi da Drescher, Guilherme Maboni, Franciele Weber, Shana de Souto Botton, Sonia Vainstein, Marilene Henning Schrank, Irene Silveira Vargas, Agueda Castagna de |
dc.subject.por.fl_str_mv |
Escherichia coli Fatores de virulência Resistência antimicrobiana |
topic |
Escherichia coli Fatores de virulência Resistência antimicrobiana E. coli Plasmids Virulence factors Antimicrobial resistance Swine |
dc.subject.eng.fl_str_mv |
E. coli Plasmids Virulence factors Antimicrobial resistance Swine |
description |
The present study determined the molecular and resistance patterns of E. coli isolates from urinary tract of swine in Southern of Brazil. Molecular characterization of urinary vesicle samples was performed by PCR detection of virulence factors from ETEC, STEC and UPEC. From a total of 82 E. coli isolates, 34 (38.63%) harbored one or more virulence factors. The frequency of virulence factors genes detected by PCR were: pap (10.97%), hlyA (10.97%), iha (9.75%), lt (8.53%), sta (7.31%) sfa (6.09%), f4 (4.87%), f5 (4.87%), stb (4.87%), f6 (1.21%) and f41 (1.21%). Isolates were resistant to penicillin (95.12%), lincomycin (93.9%), erythromycin (92.68%), tetracycline (90.24%), amoxicillin (82.92%), ampicillin (74.39%), josamycin (79.26%), norfloxacin (58.53%), enrofloxacin (57.31%), gentamicin (39.02%), neomycin (37.8%), apramycin (30.48%), colistine (30.48%) and cefalexin (6.09%). A number of 32 (39.02%) E. coli isolates harbored plasmids. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008 |
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2010-04-16T09:16:51Z |
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1517-8382 |
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000719330 |
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http://hdl.handle.net/10183/20838 |
dc.language.iso.fl_str_mv |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of microbiology. São Paulo. Vol. 39, no. 4 (Dec. 2008), p. 741-743 |
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