Citotoxic activity evaluation of essential oils and nanoemulsions of drimys angustifolia and d. brasiliensis on human glioblastoma (u-138 mg) and human bladder carcinoma (t24) cell lines in vitro

Detalhes bibliográficos
Autor(a) principal: Gomes, Madson Ralide Fonseca
Data de Publicação: 2013
Outros Autores: Schuh, Roselena Silvestri, Jacques, Ana Laura Bemvenuti, Augustin, Otávio Américo, Bordignon, Sergio Augusto de Loreto, Dias, Daiane de Oliveira, Kelmann, Regina Gendzelevski, Koester, Leticia Scherer, Gehring, Marina Petersen, Morrone, Fernanda Bueno, Campos, Maria Martha, Limberger, Renata Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/72266
Resumo: The species Drimys angustifolia Miers and D. brasiliensis Miers, commonly known as "casca-de-anta", have in their leaves essential oils that can confer cytotoxic effects. In this study, we evaluated the citotoxic effects of the volatile oils from these two species. We also proposed a nanoemulsion formulation for each of the species and assessed the in vitro cytotoxicity on U-138 MG (human glioblastoma) and T24 (human bladder carcinoma) cell lines. The plant chemical composition was evaluated by gas chromatography coupled to mass spectrometer. Furthermore, the nanoemulsions were prepared and characterized. Our results showed that; bicyclogermacrene (19.6%) and cyclocolorenone (18.2%) were the most abundant for the D angustifolia oil and D brasiliensis oil, respectively. Both nanoemulsions, D angustifolia and D brasiliensis appeared macroscopically homogeneous and opalescent bluish liquids, with nanometric mean diameters of 168 nm for D brasiliensis and 181 nm for D angustifolia. The polydispersity indices were below 0.10, with an acid pH of 4.7-6.3, and negative zeta potentials about -34 mV. The results of transmission electron microscopy showed that droplets are present in the nanometer range. Only the D brasiliensis oil was efficient in reducing the cell viability of both U-138 MG (42.5%±7.0 and 67.8%±7.8) and T24 (33.2%±2.8, 60.3%±1.6 and 80.5%±8.8) cell lines, as assessed by MTT assay. Noteworthy, similar results were obtained with cell counting. Finally, D brasiliensis oil incubation caused an increase of annexin-V and propidium iodite population, according to evaluation by cytometry analysis, what is characteristic of late apoptosis. The results presented herein lead us to consider the potential therapeutic effects of the essential oils and nanoformulations as novel strategies to inhibit tumor growth.
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spelling Gomes, Madson Ralide FonsecaSchuh, Roselena SilvestriJacques, Ana Laura BemvenutiAugustin, Otávio AméricoBordignon, Sergio Augusto de LoretoDias, Daiane de OliveiraKelmann, Regina GendzelevskiKoester, Leticia SchererGehring, Marina PetersenMorrone, Fernanda BuenoCampos, Maria MarthaLimberger, Renata Pereira2013-06-07T01:45:15Z20130102-695Xhttp://hdl.handle.net/10183/72266000876993The species Drimys angustifolia Miers and D. brasiliensis Miers, commonly known as "casca-de-anta", have in their leaves essential oils that can confer cytotoxic effects. In this study, we evaluated the citotoxic effects of the volatile oils from these two species. We also proposed a nanoemulsion formulation for each of the species and assessed the in vitro cytotoxicity on U-138 MG (human glioblastoma) and T24 (human bladder carcinoma) cell lines. The plant chemical composition was evaluated by gas chromatography coupled to mass spectrometer. Furthermore, the nanoemulsions were prepared and characterized. Our results showed that; bicyclogermacrene (19.6%) and cyclocolorenone (18.2%) were the most abundant for the D angustifolia oil and D brasiliensis oil, respectively. Both nanoemulsions, D angustifolia and D brasiliensis appeared macroscopically homogeneous and opalescent bluish liquids, with nanometric mean diameters of 168 nm for D brasiliensis and 181 nm for D angustifolia. The polydispersity indices were below 0.10, with an acid pH of 4.7-6.3, and negative zeta potentials about -34 mV. The results of transmission electron microscopy showed that droplets are present in the nanometer range. Only the D brasiliensis oil was efficient in reducing the cell viability of both U-138 MG (42.5%±7.0 and 67.8%±7.8) and T24 (33.2%±2.8, 60.3%±1.6 and 80.5%±8.8) cell lines, as assessed by MTT assay. Noteworthy, similar results were obtained with cell counting. Finally, D brasiliensis oil incubation caused an increase of annexin-V and propidium iodite population, according to evaluation by cytometry analysis, what is characteristic of late apoptosis. The results presented herein lead us to consider the potential therapeutic effects of the essential oils and nanoformulations as novel strategies to inhibit tumor growth.application/pdfengRevista brasileira de farmacognosia. Vol. 23, n. 2 (mar./abr. 2013), p. 259-267Drimys angustifoliaDrimys brasiliensisÓleos essenciaisBladder carcinomaDrimys angustifoliaDrimys brasiliensisEssential oilsGlioblastomaNanoemulsionsCitotoxic activity evaluation of essential oils and nanoemulsions of drimys angustifolia and d. brasiliensis on human glioblastoma (u-138 mg) and human bladder carcinoma (t24) cell lines in vitroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000876993.pdf000876993.pdfTexto completo (inglês)application/pdf971744http://www.lume.ufrgs.br/bitstream/10183/72266/1/000876993.pdf25ca3781029e38b25504435af2a57f88MD51TEXT000876993.pdf.txt000876993.pdf.txtExtracted Texttext/plain38398http://www.lume.ufrgs.br/bitstream/10183/72266/2/000876993.pdf.txt856b766a533d7848f135655ec10fe609MD52THUMBNAIL000876993.pdf.jpg000876993.pdf.jpgGenerated Thumbnailimage/jpeg2063http://www.lume.ufrgs.br/bitstream/10183/72266/3/000876993.pdf.jpg746de8b90cfad3296ecf09a4c47f8106MD5310183/722662018-10-05 07:57:14.86oai:www.lume.ufrgs.br:10183/72266Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-05T10:57:14Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Citotoxic activity evaluation of essential oils and nanoemulsions of drimys angustifolia and d. brasiliensis on human glioblastoma (u-138 mg) and human bladder carcinoma (t24) cell lines in vitro
title Citotoxic activity evaluation of essential oils and nanoemulsions of drimys angustifolia and d. brasiliensis on human glioblastoma (u-138 mg) and human bladder carcinoma (t24) cell lines in vitro
spellingShingle Citotoxic activity evaluation of essential oils and nanoemulsions of drimys angustifolia and d. brasiliensis on human glioblastoma (u-138 mg) and human bladder carcinoma (t24) cell lines in vitro
Gomes, Madson Ralide Fonseca
Drimys angustifolia
Drimys brasiliensis
Óleos essenciais
Bladder carcinoma
Drimys angustifolia
Drimys brasiliensis
Essential oils
Glioblastoma
Nanoemulsions
title_short Citotoxic activity evaluation of essential oils and nanoemulsions of drimys angustifolia and d. brasiliensis on human glioblastoma (u-138 mg) and human bladder carcinoma (t24) cell lines in vitro
title_full Citotoxic activity evaluation of essential oils and nanoemulsions of drimys angustifolia and d. brasiliensis on human glioblastoma (u-138 mg) and human bladder carcinoma (t24) cell lines in vitro
title_fullStr Citotoxic activity evaluation of essential oils and nanoemulsions of drimys angustifolia and d. brasiliensis on human glioblastoma (u-138 mg) and human bladder carcinoma (t24) cell lines in vitro
title_full_unstemmed Citotoxic activity evaluation of essential oils and nanoemulsions of drimys angustifolia and d. brasiliensis on human glioblastoma (u-138 mg) and human bladder carcinoma (t24) cell lines in vitro
title_sort Citotoxic activity evaluation of essential oils and nanoemulsions of drimys angustifolia and d. brasiliensis on human glioblastoma (u-138 mg) and human bladder carcinoma (t24) cell lines in vitro
author Gomes, Madson Ralide Fonseca
author_facet Gomes, Madson Ralide Fonseca
Schuh, Roselena Silvestri
Jacques, Ana Laura Bemvenuti
Augustin, Otávio Américo
Bordignon, Sergio Augusto de Loreto
Dias, Daiane de Oliveira
Kelmann, Regina Gendzelevski
Koester, Leticia Scherer
Gehring, Marina Petersen
Morrone, Fernanda Bueno
Campos, Maria Martha
Limberger, Renata Pereira
author_role author
author2 Schuh, Roselena Silvestri
Jacques, Ana Laura Bemvenuti
Augustin, Otávio Américo
Bordignon, Sergio Augusto de Loreto
Dias, Daiane de Oliveira
Kelmann, Regina Gendzelevski
Koester, Leticia Scherer
Gehring, Marina Petersen
Morrone, Fernanda Bueno
Campos, Maria Martha
Limberger, Renata Pereira
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gomes, Madson Ralide Fonseca
Schuh, Roselena Silvestri
Jacques, Ana Laura Bemvenuti
Augustin, Otávio Américo
Bordignon, Sergio Augusto de Loreto
Dias, Daiane de Oliveira
Kelmann, Regina Gendzelevski
Koester, Leticia Scherer
Gehring, Marina Petersen
Morrone, Fernanda Bueno
Campos, Maria Martha
Limberger, Renata Pereira
dc.subject.por.fl_str_mv Drimys angustifolia
Drimys brasiliensis
Óleos essenciais
topic Drimys angustifolia
Drimys brasiliensis
Óleos essenciais
Bladder carcinoma
Drimys angustifolia
Drimys brasiliensis
Essential oils
Glioblastoma
Nanoemulsions
dc.subject.eng.fl_str_mv Bladder carcinoma
Drimys angustifolia
Drimys brasiliensis
Essential oils
Glioblastoma
Nanoemulsions
description The species Drimys angustifolia Miers and D. brasiliensis Miers, commonly known as "casca-de-anta", have in their leaves essential oils that can confer cytotoxic effects. In this study, we evaluated the citotoxic effects of the volatile oils from these two species. We also proposed a nanoemulsion formulation for each of the species and assessed the in vitro cytotoxicity on U-138 MG (human glioblastoma) and T24 (human bladder carcinoma) cell lines. The plant chemical composition was evaluated by gas chromatography coupled to mass spectrometer. Furthermore, the nanoemulsions were prepared and characterized. Our results showed that; bicyclogermacrene (19.6%) and cyclocolorenone (18.2%) were the most abundant for the D angustifolia oil and D brasiliensis oil, respectively. Both nanoemulsions, D angustifolia and D brasiliensis appeared macroscopically homogeneous and opalescent bluish liquids, with nanometric mean diameters of 168 nm for D brasiliensis and 181 nm for D angustifolia. The polydispersity indices were below 0.10, with an acid pH of 4.7-6.3, and negative zeta potentials about -34 mV. The results of transmission electron microscopy showed that droplets are present in the nanometer range. Only the D brasiliensis oil was efficient in reducing the cell viability of both U-138 MG (42.5%±7.0 and 67.8%±7.8) and T24 (33.2%±2.8, 60.3%±1.6 and 80.5%±8.8) cell lines, as assessed by MTT assay. Noteworthy, similar results were obtained with cell counting. Finally, D brasiliensis oil incubation caused an increase of annexin-V and propidium iodite population, according to evaluation by cytometry analysis, what is characteristic of late apoptosis. The results presented herein lead us to consider the potential therapeutic effects of the essential oils and nanoformulations as novel strategies to inhibit tumor growth.
publishDate 2013
dc.date.accessioned.fl_str_mv 2013-06-07T01:45:15Z
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dc.relation.ispartof.pt_BR.fl_str_mv Revista brasileira de farmacognosia. Vol. 23, n. 2 (mar./abr. 2013), p. 259-267
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