Citotoxic activity evaluation of essential oils and nanoemulsions of drimys angustifolia and d. brasiliensis on human glioblastoma (u-138 mg) and human bladder carcinoma (t24) cell lines in vitro
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/72266 |
Resumo: | The species Drimys angustifolia Miers and D. brasiliensis Miers, commonly known as "casca-de-anta", have in their leaves essential oils that can confer cytotoxic effects. In this study, we evaluated the citotoxic effects of the volatile oils from these two species. We also proposed a nanoemulsion formulation for each of the species and assessed the in vitro cytotoxicity on U-138 MG (human glioblastoma) and T24 (human bladder carcinoma) cell lines. The plant chemical composition was evaluated by gas chromatography coupled to mass spectrometer. Furthermore, the nanoemulsions were prepared and characterized. Our results showed that; bicyclogermacrene (19.6%) and cyclocolorenone (18.2%) were the most abundant for the D angustifolia oil and D brasiliensis oil, respectively. Both nanoemulsions, D angustifolia and D brasiliensis appeared macroscopically homogeneous and opalescent bluish liquids, with nanometric mean diameters of 168 nm for D brasiliensis and 181 nm for D angustifolia. The polydispersity indices were below 0.10, with an acid pH of 4.7-6.3, and negative zeta potentials about -34 mV. The results of transmission electron microscopy showed that droplets are present in the nanometer range. Only the D brasiliensis oil was efficient in reducing the cell viability of both U-138 MG (42.5%±7.0 and 67.8%±7.8) and T24 (33.2%±2.8, 60.3%±1.6 and 80.5%±8.8) cell lines, as assessed by MTT assay. Noteworthy, similar results were obtained with cell counting. Finally, D brasiliensis oil incubation caused an increase of annexin-V and propidium iodite population, according to evaluation by cytometry analysis, what is characteristic of late apoptosis. The results presented herein lead us to consider the potential therapeutic effects of the essential oils and nanoformulations as novel strategies to inhibit tumor growth. |
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Gomes, Madson Ralide FonsecaSchuh, Roselena SilvestriJacques, Ana Laura BemvenutiAugustin, Otávio AméricoBordignon, Sergio Augusto de LoretoDias, Daiane de OliveiraKelmann, Regina GendzelevskiKoester, Leticia SchererGehring, Marina PetersenMorrone, Fernanda BuenoCampos, Maria MarthaLimberger, Renata Pereira2013-06-07T01:45:15Z20130102-695Xhttp://hdl.handle.net/10183/72266000876993The species Drimys angustifolia Miers and D. brasiliensis Miers, commonly known as "casca-de-anta", have in their leaves essential oils that can confer cytotoxic effects. In this study, we evaluated the citotoxic effects of the volatile oils from these two species. We also proposed a nanoemulsion formulation for each of the species and assessed the in vitro cytotoxicity on U-138 MG (human glioblastoma) and T24 (human bladder carcinoma) cell lines. The plant chemical composition was evaluated by gas chromatography coupled to mass spectrometer. Furthermore, the nanoemulsions were prepared and characterized. Our results showed that; bicyclogermacrene (19.6%) and cyclocolorenone (18.2%) were the most abundant for the D angustifolia oil and D brasiliensis oil, respectively. Both nanoemulsions, D angustifolia and D brasiliensis appeared macroscopically homogeneous and opalescent bluish liquids, with nanometric mean diameters of 168 nm for D brasiliensis and 181 nm for D angustifolia. The polydispersity indices were below 0.10, with an acid pH of 4.7-6.3, and negative zeta potentials about -34 mV. The results of transmission electron microscopy showed that droplets are present in the nanometer range. Only the D brasiliensis oil was efficient in reducing the cell viability of both U-138 MG (42.5%±7.0 and 67.8%±7.8) and T24 (33.2%±2.8, 60.3%±1.6 and 80.5%±8.8) cell lines, as assessed by MTT assay. Noteworthy, similar results were obtained with cell counting. Finally, D brasiliensis oil incubation caused an increase of annexin-V and propidium iodite population, according to evaluation by cytometry analysis, what is characteristic of late apoptosis. The results presented herein lead us to consider the potential therapeutic effects of the essential oils and nanoformulations as novel strategies to inhibit tumor growth.application/pdfengRevista brasileira de farmacognosia. Vol. 23, n. 2 (mar./abr. 2013), p. 259-267Drimys angustifoliaDrimys brasiliensisÓleos essenciaisBladder carcinomaDrimys angustifoliaDrimys brasiliensisEssential oilsGlioblastomaNanoemulsionsCitotoxic activity evaluation of essential oils and nanoemulsions of drimys angustifolia and d. brasiliensis on human glioblastoma (u-138 mg) and human bladder carcinoma (t24) cell lines in vitroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000876993.pdf000876993.pdfTexto completo (inglês)application/pdf971744http://www.lume.ufrgs.br/bitstream/10183/72266/1/000876993.pdf25ca3781029e38b25504435af2a57f88MD51TEXT000876993.pdf.txt000876993.pdf.txtExtracted Texttext/plain38398http://www.lume.ufrgs.br/bitstream/10183/72266/2/000876993.pdf.txt856b766a533d7848f135655ec10fe609MD52THUMBNAIL000876993.pdf.jpg000876993.pdf.jpgGenerated Thumbnailimage/jpeg2063http://www.lume.ufrgs.br/bitstream/10183/72266/3/000876993.pdf.jpg746de8b90cfad3296ecf09a4c47f8106MD5310183/722662018-10-05 07:57:14.86oai:www.lume.ufrgs.br:10183/72266Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-05T10:57:14Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Citotoxic activity evaluation of essential oils and nanoemulsions of drimys angustifolia and d. brasiliensis on human glioblastoma (u-138 mg) and human bladder carcinoma (t24) cell lines in vitro |
title |
Citotoxic activity evaluation of essential oils and nanoemulsions of drimys angustifolia and d. brasiliensis on human glioblastoma (u-138 mg) and human bladder carcinoma (t24) cell lines in vitro |
spellingShingle |
Citotoxic activity evaluation of essential oils and nanoemulsions of drimys angustifolia and d. brasiliensis on human glioblastoma (u-138 mg) and human bladder carcinoma (t24) cell lines in vitro Gomes, Madson Ralide Fonseca Drimys angustifolia Drimys brasiliensis Óleos essenciais Bladder carcinoma Drimys angustifolia Drimys brasiliensis Essential oils Glioblastoma Nanoemulsions |
title_short |
Citotoxic activity evaluation of essential oils and nanoemulsions of drimys angustifolia and d. brasiliensis on human glioblastoma (u-138 mg) and human bladder carcinoma (t24) cell lines in vitro |
title_full |
Citotoxic activity evaluation of essential oils and nanoemulsions of drimys angustifolia and d. brasiliensis on human glioblastoma (u-138 mg) and human bladder carcinoma (t24) cell lines in vitro |
title_fullStr |
Citotoxic activity evaluation of essential oils and nanoemulsions of drimys angustifolia and d. brasiliensis on human glioblastoma (u-138 mg) and human bladder carcinoma (t24) cell lines in vitro |
title_full_unstemmed |
Citotoxic activity evaluation of essential oils and nanoemulsions of drimys angustifolia and d. brasiliensis on human glioblastoma (u-138 mg) and human bladder carcinoma (t24) cell lines in vitro |
title_sort |
Citotoxic activity evaluation of essential oils and nanoemulsions of drimys angustifolia and d. brasiliensis on human glioblastoma (u-138 mg) and human bladder carcinoma (t24) cell lines in vitro |
author |
Gomes, Madson Ralide Fonseca |
author_facet |
Gomes, Madson Ralide Fonseca Schuh, Roselena Silvestri Jacques, Ana Laura Bemvenuti Augustin, Otávio Américo Bordignon, Sergio Augusto de Loreto Dias, Daiane de Oliveira Kelmann, Regina Gendzelevski Koester, Leticia Scherer Gehring, Marina Petersen Morrone, Fernanda Bueno Campos, Maria Martha Limberger, Renata Pereira |
author_role |
author |
author2 |
Schuh, Roselena Silvestri Jacques, Ana Laura Bemvenuti Augustin, Otávio Américo Bordignon, Sergio Augusto de Loreto Dias, Daiane de Oliveira Kelmann, Regina Gendzelevski Koester, Leticia Scherer Gehring, Marina Petersen Morrone, Fernanda Bueno Campos, Maria Martha Limberger, Renata Pereira |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Gomes, Madson Ralide Fonseca Schuh, Roselena Silvestri Jacques, Ana Laura Bemvenuti Augustin, Otávio Américo Bordignon, Sergio Augusto de Loreto Dias, Daiane de Oliveira Kelmann, Regina Gendzelevski Koester, Leticia Scherer Gehring, Marina Petersen Morrone, Fernanda Bueno Campos, Maria Martha Limberger, Renata Pereira |
dc.subject.por.fl_str_mv |
Drimys angustifolia Drimys brasiliensis Óleos essenciais |
topic |
Drimys angustifolia Drimys brasiliensis Óleos essenciais Bladder carcinoma Drimys angustifolia Drimys brasiliensis Essential oils Glioblastoma Nanoemulsions |
dc.subject.eng.fl_str_mv |
Bladder carcinoma Drimys angustifolia Drimys brasiliensis Essential oils Glioblastoma Nanoemulsions |
description |
The species Drimys angustifolia Miers and D. brasiliensis Miers, commonly known as "casca-de-anta", have in their leaves essential oils that can confer cytotoxic effects. In this study, we evaluated the citotoxic effects of the volatile oils from these two species. We also proposed a nanoemulsion formulation for each of the species and assessed the in vitro cytotoxicity on U-138 MG (human glioblastoma) and T24 (human bladder carcinoma) cell lines. The plant chemical composition was evaluated by gas chromatography coupled to mass spectrometer. Furthermore, the nanoemulsions were prepared and characterized. Our results showed that; bicyclogermacrene (19.6%) and cyclocolorenone (18.2%) were the most abundant for the D angustifolia oil and D brasiliensis oil, respectively. Both nanoemulsions, D angustifolia and D brasiliensis appeared macroscopically homogeneous and opalescent bluish liquids, with nanometric mean diameters of 168 nm for D brasiliensis and 181 nm for D angustifolia. The polydispersity indices were below 0.10, with an acid pH of 4.7-6.3, and negative zeta potentials about -34 mV. The results of transmission electron microscopy showed that droplets are present in the nanometer range. Only the D brasiliensis oil was efficient in reducing the cell viability of both U-138 MG (42.5%±7.0 and 67.8%±7.8) and T24 (33.2%±2.8, 60.3%±1.6 and 80.5%±8.8) cell lines, as assessed by MTT assay. Noteworthy, similar results were obtained with cell counting. Finally, D brasiliensis oil incubation caused an increase of annexin-V and propidium iodite population, according to evaluation by cytometry analysis, what is characteristic of late apoptosis. The results presented herein lead us to consider the potential therapeutic effects of the essential oils and nanoformulations as novel strategies to inhibit tumor growth. |
publishDate |
2013 |
dc.date.accessioned.fl_str_mv |
2013-06-07T01:45:15Z |
dc.date.issued.fl_str_mv |
2013 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
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0102-695X |
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000876993 |
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http://hdl.handle.net/10183/72266 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Revista brasileira de farmacognosia. Vol. 23, n. 2 (mar./abr. 2013), p. 259-267 |
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