Nanoformulation shows cytotoxicity against glioblastoma cell lines and antiangiogenic activity in chicken chorioallantoic membrane

Detalhes bibliográficos
Autor(a) principal: Dallemole, Danieli Rosane
Data de Publicação: 2021
Outros Autores: Terroso, Thatiana Ferreira, Alves, Aline de Cristo Soares, Scholl, Juliete Nathali, Onzi, Giovana Ravizzoni, Cé, Rodrigo, Paese, Karina, Battastini, Ana Maria Oliveira, Guterres, Silvia Stanisçuaski, Figueiró, Fabrício, Pohlmann, Adriana Raffin
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/229504
Resumo: Glioblastoma (GB) is a histological and genetically heterogeneous brain tumor that is highly proliferative and vascularized. The prognosis is poor with currently available treatment. In this study, we evaluated the cytotoxicity and antiangiogenic activity of doxorubicin-loaded-chitosan-coated-arginylglycylaspartic acid-functionalized-poly(ε-caprolactone)-alpha bisabolol-LNC (AB-DOX-LNC-L-C-RGD). The nanoformulation was prepared by self-assembling followed by interfacial reactions, physicochemically characterized and evaluated in vitro against GB cell lines (U87MG and U138MG) and in vivo using the chicken chorioallantoic membrane assay (CAM). Spherical shape nanocapsules had a hydrodynamic mean diameter of 138 nm, zeta potential of +13.4 mV, doxorubicin encapsulation of 65%, and RGD conjugation of 92%. After 24 h of treatment (U87MG and U138MG), the median inhibition concentrations (IC50) were 520 and 490 nmol L−1 doxorubicin-equivalent concentrations, respectively. The treatment induced antiproliferative activity with S-phase cell-cycle arrest and apoptosis in the GB cells. Furthermore, after 48 h of exposure, evaluation of antiangiogenic activity (CAM) showed that the relative vessel growth following treatment with the nanocapsules was 5.4 times lower than that with the control treatment. The results support the therapeutic potential of the nanoformulation against GB and, thereby, pave the way for future preclinical studies.
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spelling Dallemole, Danieli RosaneTerroso, Thatiana FerreiraAlves, Aline de Cristo SoaresScholl, Juliete NathaliOnzi, Giovana RavizzoniCé, RodrigoPaese, KarinaBattastini, Ana Maria OliveiraGuterres, Silvia StanisçuaskiFigueiró, FabrícioPohlmann, Adriana Raffin2021-09-03T04:24:20Z20211999-4923http://hdl.handle.net/10183/229504001131042Glioblastoma (GB) is a histological and genetically heterogeneous brain tumor that is highly proliferative and vascularized. The prognosis is poor with currently available treatment. In this study, we evaluated the cytotoxicity and antiangiogenic activity of doxorubicin-loaded-chitosan-coated-arginylglycylaspartic acid-functionalized-poly(ε-caprolactone)-alpha bisabolol-LNC (AB-DOX-LNC-L-C-RGD). The nanoformulation was prepared by self-assembling followed by interfacial reactions, physicochemically characterized and evaluated in vitro against GB cell lines (U87MG and U138MG) and in vivo using the chicken chorioallantoic membrane assay (CAM). Spherical shape nanocapsules had a hydrodynamic mean diameter of 138 nm, zeta potential of +13.4 mV, doxorubicin encapsulation of 65%, and RGD conjugation of 92%. After 24 h of treatment (U87MG and U138MG), the median inhibition concentrations (IC50) were 520 and 490 nmol L−1 doxorubicin-equivalent concentrations, respectively. The treatment induced antiproliferative activity with S-phase cell-cycle arrest and apoptosis in the GB cells. Furthermore, after 48 h of exposure, evaluation of antiangiogenic activity (CAM) showed that the relative vessel growth following treatment with the nanocapsules was 5.4 times lower than that with the control treatment. The results support the therapeutic potential of the nanoformulation against GB and, thereby, pave the way for future preclinical studies.application/pdfengPharmaceutics. Basel. Vol. 13, no. 6 (June 2021), 862, 20 p.Membrana corioalantoideGalinhasGlioblastomaLinhagem celularNanocápsulasNeovascularização patológicaDoxorrubicinaÁcido aspárticoGlioblastomaMulti-drug delivery systemsLipid-core nanocapsulesSurface functionalizationCAM assayNanoformulation shows cytotoxicity against glioblastoma cell lines and antiangiogenic activity in chicken chorioallantoic membraneEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001131042.pdf.txt001131042.pdf.txtExtracted Texttext/plain74340http://www.lume.ufrgs.br/bitstream/10183/229504/2/001131042.pdf.txta98c8d14ca1a1d2eaef8eaf22154a7ffMD52ORIGINAL001131042.pdfTexto completo (inglês)application/pdf5145001http://www.lume.ufrgs.br/bitstream/10183/229504/1/001131042.pdf3ba71b2da05ce8e026bce033ec86a810MD5110183/2295042021-09-19 04:28:00.674169oai:www.lume.ufrgs.br:10183/229504Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-09-19T07:28Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Nanoformulation shows cytotoxicity against glioblastoma cell lines and antiangiogenic activity in chicken chorioallantoic membrane
title Nanoformulation shows cytotoxicity against glioblastoma cell lines and antiangiogenic activity in chicken chorioallantoic membrane
spellingShingle Nanoformulation shows cytotoxicity against glioblastoma cell lines and antiangiogenic activity in chicken chorioallantoic membrane
Dallemole, Danieli Rosane
Membrana corioalantoide
Galinhas
Glioblastoma
Linhagem celular
Nanocápsulas
Neovascularização patológica
Doxorrubicina
Ácido aspártico
Glioblastoma
Multi-drug delivery systems
Lipid-core nanocapsules
Surface functionalization
CAM assay
title_short Nanoformulation shows cytotoxicity against glioblastoma cell lines and antiangiogenic activity in chicken chorioallantoic membrane
title_full Nanoformulation shows cytotoxicity against glioblastoma cell lines and antiangiogenic activity in chicken chorioallantoic membrane
title_fullStr Nanoformulation shows cytotoxicity against glioblastoma cell lines and antiangiogenic activity in chicken chorioallantoic membrane
title_full_unstemmed Nanoformulation shows cytotoxicity against glioblastoma cell lines and antiangiogenic activity in chicken chorioallantoic membrane
title_sort Nanoformulation shows cytotoxicity against glioblastoma cell lines and antiangiogenic activity in chicken chorioallantoic membrane
author Dallemole, Danieli Rosane
author_facet Dallemole, Danieli Rosane
Terroso, Thatiana Ferreira
Alves, Aline de Cristo Soares
Scholl, Juliete Nathali
Onzi, Giovana Ravizzoni
Cé, Rodrigo
Paese, Karina
Battastini, Ana Maria Oliveira
Guterres, Silvia Stanisçuaski
Figueiró, Fabrício
Pohlmann, Adriana Raffin
author_role author
author2 Terroso, Thatiana Ferreira
Alves, Aline de Cristo Soares
Scholl, Juliete Nathali
Onzi, Giovana Ravizzoni
Cé, Rodrigo
Paese, Karina
Battastini, Ana Maria Oliveira
Guterres, Silvia Stanisçuaski
Figueiró, Fabrício
Pohlmann, Adriana Raffin
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Dallemole, Danieli Rosane
Terroso, Thatiana Ferreira
Alves, Aline de Cristo Soares
Scholl, Juliete Nathali
Onzi, Giovana Ravizzoni
Cé, Rodrigo
Paese, Karina
Battastini, Ana Maria Oliveira
Guterres, Silvia Stanisçuaski
Figueiró, Fabrício
Pohlmann, Adriana Raffin
dc.subject.por.fl_str_mv Membrana corioalantoide
Galinhas
Glioblastoma
Linhagem celular
Nanocápsulas
Neovascularização patológica
Doxorrubicina
Ácido aspártico
topic Membrana corioalantoide
Galinhas
Glioblastoma
Linhagem celular
Nanocápsulas
Neovascularização patológica
Doxorrubicina
Ácido aspártico
Glioblastoma
Multi-drug delivery systems
Lipid-core nanocapsules
Surface functionalization
CAM assay
dc.subject.eng.fl_str_mv Glioblastoma
Multi-drug delivery systems
Lipid-core nanocapsules
Surface functionalization
CAM assay
description Glioblastoma (GB) is a histological and genetically heterogeneous brain tumor that is highly proliferative and vascularized. The prognosis is poor with currently available treatment. In this study, we evaluated the cytotoxicity and antiangiogenic activity of doxorubicin-loaded-chitosan-coated-arginylglycylaspartic acid-functionalized-poly(ε-caprolactone)-alpha bisabolol-LNC (AB-DOX-LNC-L-C-RGD). The nanoformulation was prepared by self-assembling followed by interfacial reactions, physicochemically characterized and evaluated in vitro against GB cell lines (U87MG and U138MG) and in vivo using the chicken chorioallantoic membrane assay (CAM). Spherical shape nanocapsules had a hydrodynamic mean diameter of 138 nm, zeta potential of +13.4 mV, doxorubicin encapsulation of 65%, and RGD conjugation of 92%. After 24 h of treatment (U87MG and U138MG), the median inhibition concentrations (IC50) were 520 and 490 nmol L−1 doxorubicin-equivalent concentrations, respectively. The treatment induced antiproliferative activity with S-phase cell-cycle arrest and apoptosis in the GB cells. Furthermore, after 48 h of exposure, evaluation of antiangiogenic activity (CAM) showed that the relative vessel growth following treatment with the nanocapsules was 5.4 times lower than that with the control treatment. The results support the therapeutic potential of the nanoformulation against GB and, thereby, pave the way for future preclinical studies.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-09-03T04:24:20Z
dc.date.issued.fl_str_mv 2021
dc.type.driver.fl_str_mv Estrangeiro
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/229504
dc.identifier.issn.pt_BR.fl_str_mv 1999-4923
dc.identifier.nrb.pt_BR.fl_str_mv 001131042
identifier_str_mv 1999-4923
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url http://hdl.handle.net/10183/229504
dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Pharmaceutics. Basel. Vol. 13, no. 6 (June 2021), 862, 20 p.
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