Nanoformulation shows cytotoxicity against glioblastoma cell lines and antiangiogenic activity in chicken chorioallantoic membrane
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/229504 |
Resumo: | Glioblastoma (GB) is a histological and genetically heterogeneous brain tumor that is highly proliferative and vascularized. The prognosis is poor with currently available treatment. In this study, we evaluated the cytotoxicity and antiangiogenic activity of doxorubicin-loaded-chitosan-coated-arginylglycylaspartic acid-functionalized-poly(ε-caprolactone)-alpha bisabolol-LNC (AB-DOX-LNC-L-C-RGD). The nanoformulation was prepared by self-assembling followed by interfacial reactions, physicochemically characterized and evaluated in vitro against GB cell lines (U87MG and U138MG) and in vivo using the chicken chorioallantoic membrane assay (CAM). Spherical shape nanocapsules had a hydrodynamic mean diameter of 138 nm, zeta potential of +13.4 mV, doxorubicin encapsulation of 65%, and RGD conjugation of 92%. After 24 h of treatment (U87MG and U138MG), the median inhibition concentrations (IC50) were 520 and 490 nmol L−1 doxorubicin-equivalent concentrations, respectively. The treatment induced antiproliferative activity with S-phase cell-cycle arrest and apoptosis in the GB cells. Furthermore, after 48 h of exposure, evaluation of antiangiogenic activity (CAM) showed that the relative vessel growth following treatment with the nanocapsules was 5.4 times lower than that with the control treatment. The results support the therapeutic potential of the nanoformulation against GB and, thereby, pave the way for future preclinical studies. |
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Dallemole, Danieli RosaneTerroso, Thatiana FerreiraAlves, Aline de Cristo SoaresScholl, Juliete NathaliOnzi, Giovana RavizzoniCé, RodrigoPaese, KarinaBattastini, Ana Maria OliveiraGuterres, Silvia StanisçuaskiFigueiró, FabrícioPohlmann, Adriana Raffin2021-09-03T04:24:20Z20211999-4923http://hdl.handle.net/10183/229504001131042Glioblastoma (GB) is a histological and genetically heterogeneous brain tumor that is highly proliferative and vascularized. The prognosis is poor with currently available treatment. In this study, we evaluated the cytotoxicity and antiangiogenic activity of doxorubicin-loaded-chitosan-coated-arginylglycylaspartic acid-functionalized-poly(ε-caprolactone)-alpha bisabolol-LNC (AB-DOX-LNC-L-C-RGD). The nanoformulation was prepared by self-assembling followed by interfacial reactions, physicochemically characterized and evaluated in vitro against GB cell lines (U87MG and U138MG) and in vivo using the chicken chorioallantoic membrane assay (CAM). Spherical shape nanocapsules had a hydrodynamic mean diameter of 138 nm, zeta potential of +13.4 mV, doxorubicin encapsulation of 65%, and RGD conjugation of 92%. After 24 h of treatment (U87MG and U138MG), the median inhibition concentrations (IC50) were 520 and 490 nmol L−1 doxorubicin-equivalent concentrations, respectively. The treatment induced antiproliferative activity with S-phase cell-cycle arrest and apoptosis in the GB cells. Furthermore, after 48 h of exposure, evaluation of antiangiogenic activity (CAM) showed that the relative vessel growth following treatment with the nanocapsules was 5.4 times lower than that with the control treatment. The results support the therapeutic potential of the nanoformulation against GB and, thereby, pave the way for future preclinical studies.application/pdfengPharmaceutics. Basel. Vol. 13, no. 6 (June 2021), 862, 20 p.Membrana corioalantoideGalinhasGlioblastomaLinhagem celularNanocápsulasNeovascularização patológicaDoxorrubicinaÁcido aspárticoGlioblastomaMulti-drug delivery systemsLipid-core nanocapsulesSurface functionalizationCAM assayNanoformulation shows cytotoxicity against glioblastoma cell lines and antiangiogenic activity in chicken chorioallantoic membraneEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001131042.pdf.txt001131042.pdf.txtExtracted Texttext/plain74340http://www.lume.ufrgs.br/bitstream/10183/229504/2/001131042.pdf.txta98c8d14ca1a1d2eaef8eaf22154a7ffMD52ORIGINAL001131042.pdfTexto completo (inglês)application/pdf5145001http://www.lume.ufrgs.br/bitstream/10183/229504/1/001131042.pdf3ba71b2da05ce8e026bce033ec86a810MD5110183/2295042021-09-19 04:28:00.674169oai:www.lume.ufrgs.br:10183/229504Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-09-19T07:28Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Nanoformulation shows cytotoxicity against glioblastoma cell lines and antiangiogenic activity in chicken chorioallantoic membrane |
title |
Nanoformulation shows cytotoxicity against glioblastoma cell lines and antiangiogenic activity in chicken chorioallantoic membrane |
spellingShingle |
Nanoformulation shows cytotoxicity against glioblastoma cell lines and antiangiogenic activity in chicken chorioallantoic membrane Dallemole, Danieli Rosane Membrana corioalantoide Galinhas Glioblastoma Linhagem celular Nanocápsulas Neovascularização patológica Doxorrubicina Ácido aspártico Glioblastoma Multi-drug delivery systems Lipid-core nanocapsules Surface functionalization CAM assay |
title_short |
Nanoformulation shows cytotoxicity against glioblastoma cell lines and antiangiogenic activity in chicken chorioallantoic membrane |
title_full |
Nanoformulation shows cytotoxicity against glioblastoma cell lines and antiangiogenic activity in chicken chorioallantoic membrane |
title_fullStr |
Nanoformulation shows cytotoxicity against glioblastoma cell lines and antiangiogenic activity in chicken chorioallantoic membrane |
title_full_unstemmed |
Nanoformulation shows cytotoxicity against glioblastoma cell lines and antiangiogenic activity in chicken chorioallantoic membrane |
title_sort |
Nanoformulation shows cytotoxicity against glioblastoma cell lines and antiangiogenic activity in chicken chorioallantoic membrane |
author |
Dallemole, Danieli Rosane |
author_facet |
Dallemole, Danieli Rosane Terroso, Thatiana Ferreira Alves, Aline de Cristo Soares Scholl, Juliete Nathali Onzi, Giovana Ravizzoni Cé, Rodrigo Paese, Karina Battastini, Ana Maria Oliveira Guterres, Silvia Stanisçuaski Figueiró, Fabrício Pohlmann, Adriana Raffin |
author_role |
author |
author2 |
Terroso, Thatiana Ferreira Alves, Aline de Cristo Soares Scholl, Juliete Nathali Onzi, Giovana Ravizzoni Cé, Rodrigo Paese, Karina Battastini, Ana Maria Oliveira Guterres, Silvia Stanisçuaski Figueiró, Fabrício Pohlmann, Adriana Raffin |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Dallemole, Danieli Rosane Terroso, Thatiana Ferreira Alves, Aline de Cristo Soares Scholl, Juliete Nathali Onzi, Giovana Ravizzoni Cé, Rodrigo Paese, Karina Battastini, Ana Maria Oliveira Guterres, Silvia Stanisçuaski Figueiró, Fabrício Pohlmann, Adriana Raffin |
dc.subject.por.fl_str_mv |
Membrana corioalantoide Galinhas Glioblastoma Linhagem celular Nanocápsulas Neovascularização patológica Doxorrubicina Ácido aspártico |
topic |
Membrana corioalantoide Galinhas Glioblastoma Linhagem celular Nanocápsulas Neovascularização patológica Doxorrubicina Ácido aspártico Glioblastoma Multi-drug delivery systems Lipid-core nanocapsules Surface functionalization CAM assay |
dc.subject.eng.fl_str_mv |
Glioblastoma Multi-drug delivery systems Lipid-core nanocapsules Surface functionalization CAM assay |
description |
Glioblastoma (GB) is a histological and genetically heterogeneous brain tumor that is highly proliferative and vascularized. The prognosis is poor with currently available treatment. In this study, we evaluated the cytotoxicity and antiangiogenic activity of doxorubicin-loaded-chitosan-coated-arginylglycylaspartic acid-functionalized-poly(ε-caprolactone)-alpha bisabolol-LNC (AB-DOX-LNC-L-C-RGD). The nanoformulation was prepared by self-assembling followed by interfacial reactions, physicochemically characterized and evaluated in vitro against GB cell lines (U87MG and U138MG) and in vivo using the chicken chorioallantoic membrane assay (CAM). Spherical shape nanocapsules had a hydrodynamic mean diameter of 138 nm, zeta potential of +13.4 mV, doxorubicin encapsulation of 65%, and RGD conjugation of 92%. After 24 h of treatment (U87MG and U138MG), the median inhibition concentrations (IC50) were 520 and 490 nmol L−1 doxorubicin-equivalent concentrations, respectively. The treatment induced antiproliferative activity with S-phase cell-cycle arrest and apoptosis in the GB cells. Furthermore, after 48 h of exposure, evaluation of antiangiogenic activity (CAM) showed that the relative vessel growth following treatment with the nanocapsules was 5.4 times lower than that with the control treatment. The results support the therapeutic potential of the nanoformulation against GB and, thereby, pave the way for future preclinical studies. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-09-03T04:24:20Z |
dc.date.issued.fl_str_mv |
2021 |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/229504 |
dc.identifier.issn.pt_BR.fl_str_mv |
1999-4923 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001131042 |
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1999-4923 001131042 |
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http://hdl.handle.net/10183/229504 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Pharmaceutics. Basel. Vol. 13, no. 6 (June 2021), 862, 20 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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