Comparative microbiome analysis in cystic fbrosis and non-cystic fbrosis bronchiectasis

• Autor(a) principal: Souza, Heryk Motta de
• Data de Publicação: 2024
• Outros Autores: Reuwsaat, Júlia Catarina Vieira, Lopes, Fernanda Cortez, Viezzer, Graciele, Volpato, Fabiana Caroline Zempulski, Barth, Afonso Luis, Dalcin, Paulo de Tarso Roth, Staats, Charley Christian, Vainstein, Marilene Henning, Silva, Lívia Kmetzsch Rosa e
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/280612
• topic: Bronquiectasia; Fibrose cística; Bronquiectasia; Abnormal and irreversible bronchial dilation; Non-CF bronchiectasis

Background: Bronchiectasis is a condition characterized by abnormal and irreversible bronchial dilation resulting from lung tissue damage and can be categorized into two main groups: cystic fibrosis (CF) and non-CF bronchiectasis (NCFB). Both diseases are marked by recurrent infections, inflammatory exacerbations, and lung damage. Given that infections are the primary drivers of disease progression, characterization of the respiratory microbiome can shed light on compositional alterations and susceptibility to antimicrobial drugs in these cases compared to healthy individuals. Methods: To assess the microbiota in the two studied diseases, 35 subjects were recruited, comprising 10 NCFB and 13 CF patients and 12 healthy individuals. Nasopharyngeal swabs and induced sputum were collected, and total DNA was extracted. The DNA was then sequenced by the shotgun method and evaluated using the SqueezeMeta pipeline and R.