Antimicrobial resistance and molecular characterization of Salmonella enterica serotypes isolated from poultry sources in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/196140 |
Resumo: | Salmonella spp. remain among the most important agents of foodborne diseases worldwide. The importance of Salmonella spp. in public health is linked to their wide range of antimicrobial resistance and to their pathogenicity and virulence in both human and animal hosts. The aim of this study was to determine the antimicrobial resistance patterns for Salmonella serotypes isolated from poultry sources in Brazil and to detect virulence-associated genes and verify their association with specific serotypes. A total of 163 strains of Salmonella enterica isolated from poultry sources in Southern Brazil were selected, and each belonged to one of 11 different serotypes. They were tested against ten antibiotics and examined for the presence of 26 virulence-associated genes by PCR. S. Typhimurium, S. Bredeney, S. Schwarzengrund and S. Tennessee showed the highest overall resistance rates. Approximately 18% of Salmonella strains were classified as multidrug-resistant strains. Our results indicate associations between antimicrobial resistance and specific serotypes. Most of the investigated genes presented a high frequency and a regular distribution, regardless of the serotype. Eight genes are positively or negatively associated with at least one serotype. The observed associations between antimicrobial resistance and specific serotypes are useful in developing specific control and treatment measures for each serotype. Despite the virulence genes being evenly distributed among the serotypes, some of these genes are associated with specific serotypes, and sefA, sopEand lpfA were selected as possible markers of Salmonella serotypes. |
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Borges, Karen ApellanisFurian, Thales QuediSouza, Sara Neves deSalle, Carlos Tadeu PippiMoraes, Hamilton Luiz de SouzaNascimento, Vladimir Pinheiro do2019-06-22T02:35:14Z20191516-635Xhttp://hdl.handle.net/10183/196140001093789Salmonella spp. remain among the most important agents of foodborne diseases worldwide. The importance of Salmonella spp. in public health is linked to their wide range of antimicrobial resistance and to their pathogenicity and virulence in both human and animal hosts. The aim of this study was to determine the antimicrobial resistance patterns for Salmonella serotypes isolated from poultry sources in Brazil and to detect virulence-associated genes and verify their association with specific serotypes. A total of 163 strains of Salmonella enterica isolated from poultry sources in Southern Brazil were selected, and each belonged to one of 11 different serotypes. They were tested against ten antibiotics and examined for the presence of 26 virulence-associated genes by PCR. S. Typhimurium, S. Bredeney, S. Schwarzengrund and S. Tennessee showed the highest overall resistance rates. Approximately 18% of Salmonella strains were classified as multidrug-resistant strains. Our results indicate associations between antimicrobial resistance and specific serotypes. Most of the investigated genes presented a high frequency and a regular distribution, regardless of the serotype. Eight genes are positively or negatively associated with at least one serotype. The observed associations between antimicrobial resistance and specific serotypes are useful in developing specific control and treatment measures for each serotype. Despite the virulence genes being evenly distributed among the serotypes, some of these genes are associated with specific serotypes, and sefA, sopEand lpfA were selected as possible markers of Salmonella serotypes.application/pdfengRevista brasileira de ciência avícola = Brazilian journal of poultry science. Campinas, SP. Vol. 21, no. 1, (Jan./Mar. 2019), 8 p.Resistência antimicrobianaFatores de virulênciaSorogrupoSalmonella entericaAvesAntibiotic susceptibilityGenetic virulenceChickenSalmonellosisSerovarsAntimicrobial resistance and molecular characterization of Salmonella enterica serotypes isolated from poultry sources in Brazilinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001093789.pdf.txt001093789.pdf.txtExtracted Texttext/plain39630http://www.lume.ufrgs.br/bitstream/10183/196140/2/001093789.pdf.txtcaa565aeaa315d544066d6d7c5772530MD52ORIGINAL001093789.pdfTexto completo (inglês)application/pdf268632http://www.lume.ufrgs.br/bitstream/10183/196140/1/001093789.pdf59b2d37068e9cc08ed511900cc233b46MD5110183/1961402019-06-23 02:34:04.830249oai:www.lume.ufrgs.br:10183/196140Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-06-23T05:34:04Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Antimicrobial resistance and molecular characterization of Salmonella enterica serotypes isolated from poultry sources in Brazil |
title |
Antimicrobial resistance and molecular characterization of Salmonella enterica serotypes isolated from poultry sources in Brazil |
spellingShingle |
Antimicrobial resistance and molecular characterization of Salmonella enterica serotypes isolated from poultry sources in Brazil Borges, Karen Apellanis Resistência antimicrobiana Fatores de virulência Sorogrupo Salmonella enterica Aves Antibiotic susceptibility Genetic virulence Chicken Salmonellosis Serovars |
title_short |
Antimicrobial resistance and molecular characterization of Salmonella enterica serotypes isolated from poultry sources in Brazil |
title_full |
Antimicrobial resistance and molecular characterization of Salmonella enterica serotypes isolated from poultry sources in Brazil |
title_fullStr |
Antimicrobial resistance and molecular characterization of Salmonella enterica serotypes isolated from poultry sources in Brazil |
title_full_unstemmed |
Antimicrobial resistance and molecular characterization of Salmonella enterica serotypes isolated from poultry sources in Brazil |
title_sort |
Antimicrobial resistance and molecular characterization of Salmonella enterica serotypes isolated from poultry sources in Brazil |
author |
Borges, Karen Apellanis |
author_facet |
Borges, Karen Apellanis Furian, Thales Quedi Souza, Sara Neves de Salle, Carlos Tadeu Pippi Moraes, Hamilton Luiz de Souza Nascimento, Vladimir Pinheiro do |
author_role |
author |
author2 |
Furian, Thales Quedi Souza, Sara Neves de Salle, Carlos Tadeu Pippi Moraes, Hamilton Luiz de Souza Nascimento, Vladimir Pinheiro do |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Borges, Karen Apellanis Furian, Thales Quedi Souza, Sara Neves de Salle, Carlos Tadeu Pippi Moraes, Hamilton Luiz de Souza Nascimento, Vladimir Pinheiro do |
dc.subject.por.fl_str_mv |
Resistência antimicrobiana Fatores de virulência Sorogrupo Salmonella enterica Aves |
topic |
Resistência antimicrobiana Fatores de virulência Sorogrupo Salmonella enterica Aves Antibiotic susceptibility Genetic virulence Chicken Salmonellosis Serovars |
dc.subject.eng.fl_str_mv |
Antibiotic susceptibility Genetic virulence Chicken Salmonellosis Serovars |
description |
Salmonella spp. remain among the most important agents of foodborne diseases worldwide. The importance of Salmonella spp. in public health is linked to their wide range of antimicrobial resistance and to their pathogenicity and virulence in both human and animal hosts. The aim of this study was to determine the antimicrobial resistance patterns for Salmonella serotypes isolated from poultry sources in Brazil and to detect virulence-associated genes and verify their association with specific serotypes. A total of 163 strains of Salmonella enterica isolated from poultry sources in Southern Brazil were selected, and each belonged to one of 11 different serotypes. They were tested against ten antibiotics and examined for the presence of 26 virulence-associated genes by PCR. S. Typhimurium, S. Bredeney, S. Schwarzengrund and S. Tennessee showed the highest overall resistance rates. Approximately 18% of Salmonella strains were classified as multidrug-resistant strains. Our results indicate associations between antimicrobial resistance and specific serotypes. Most of the investigated genes presented a high frequency and a regular distribution, regardless of the serotype. Eight genes are positively or negatively associated with at least one serotype. The observed associations between antimicrobial resistance and specific serotypes are useful in developing specific control and treatment measures for each serotype. Despite the virulence genes being evenly distributed among the serotypes, some of these genes are associated with specific serotypes, and sefA, sopEand lpfA were selected as possible markers of Salmonella serotypes. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-06-22T02:35:14Z |
dc.date.issued.fl_str_mv |
2019 |
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info:eu-repo/semantics/article info:eu-repo/semantics/other |
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article |
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http://hdl.handle.net/10183/196140 |
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1516-635X |
dc.identifier.nrb.pt_BR.fl_str_mv |
001093789 |
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http://hdl.handle.net/10183/196140 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Revista brasileira de ciência avícola = Brazilian journal of poultry science. Campinas, SP. Vol. 21, no. 1, (Jan./Mar. 2019), 8 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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