Cryptococcus spp., identification and susceptibility to antifungals in isolated in laboratory of reference in The State of Rio Grande Do Sul, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/212593 |
Resumo: | Introduction: The Cryptococcus spp., fungus with opportunistic behavior, present in environmental sources, bird excreta, remains of trees, among others. It is inhaled by the individual, causing infection that can spread to other organs and systems with tropism through the central nervous system, causing meningoencephalitis. It is estimated that C. neoformans causes more than one million new cases of cryptococcosis per year, about 625,000 deaths. Meningoencephalitis, the most common clinical manifestation, is estimated to cause 120,000 to 240,000 deaths per year worldwide. Methods: This study was conducted from January 2012 to June 2019. Biological materials were processed, mainly the cerebrospinal fluid of patients with suspected cryptococcosis. All the phenotypically identified isolates were confirmed by the Polymerase Chain Reaction (PCR) molecular technique. The antifungals fluconazole, itraconazole and amphotericin B were also tested to verify the sensitivity profile of the isolates. Conclusions and discussion: In this study it was possible to observe and confirm a higher prevalence of Cryptococcus neoformans species than C. gattii. Azole resistance was also observed by the fungus Cryptococcus spp., suggesting a possible synergism of azoles with anti-inflammatory substances, but further studies are needed both in vitro and in vivo. The imminent biological risk from the presence of the two fungal species in the environment, including the confirmation of antifungal resistant strains, corroborates the need for continuous environmental monitoring, including the presence of new pathogenic species and consequently how to conduct treatment in the various clinic presentations by this yeast. |
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Silva, Leonardo de Paulo Recuero daGarcia, Emily dos SantosCharles, Fernanda CostaMartinez, Karina de VargasLeandro, Renata NevesGiudice, Victoria MarconNascimento, Caroline Gabriela Santos doPereira, Patrícia AbreuBorges, Júlia SpierGonçalves, Stela Maris BottinRodrigues, Diana Mara GarciaPicanço, João Marcelo AstolfiCalil, Luciane NoalMezzari, Adelina2020-08-04T03:39:12Z20202230-7605http://hdl.handle.net/10183/212593001114965Introduction: The Cryptococcus spp., fungus with opportunistic behavior, present in environmental sources, bird excreta, remains of trees, among others. It is inhaled by the individual, causing infection that can spread to other organs and systems with tropism through the central nervous system, causing meningoencephalitis. It is estimated that C. neoformans causes more than one million new cases of cryptococcosis per year, about 625,000 deaths. Meningoencephalitis, the most common clinical manifestation, is estimated to cause 120,000 to 240,000 deaths per year worldwide. Methods: This study was conducted from January 2012 to June 2019. Biological materials were processed, mainly the cerebrospinal fluid of patients with suspected cryptococcosis. All the phenotypically identified isolates were confirmed by the Polymerase Chain Reaction (PCR) molecular technique. The antifungals fluconazole, itraconazole and amphotericin B were also tested to verify the sensitivity profile of the isolates. Conclusions and discussion: In this study it was possible to observe and confirm a higher prevalence of Cryptococcus neoformans species than C. gattii. Azole resistance was also observed by the fungus Cryptococcus spp., suggesting a possible synergism of azoles with anti-inflammatory substances, but further studies are needed both in vitro and in vivo. The imminent biological risk from the presence of the two fungal species in the environment, including the confirmation of antifungal resistant strains, corroborates the need for continuous environmental monitoring, including the presence of new pathogenic species and consequently how to conduct treatment in the various clinic presentations by this yeast.application/pdfengInternational journal of pharmacy and biological sciences. Índia. Vol. 10, n. 2 (Apr. 2020), p. 128-134FarmáciaCryptococcusCryptococcus spp.IdentificationSusceptibilityRio Grande do SulCryptococcus spp., identification and susceptibility to antifungals in isolated in laboratory of reference in The State of Rio Grande Do Sul, BrazilEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001114965.pdf.txt001114965.pdf.txtExtracted Texttext/plain25015http://www.lume.ufrgs.br/bitstream/10183/212593/2/001114965.pdf.txt495e7c242097896a80112374ba8e36c2MD52ORIGINAL001114965.pdfTexto completo (inglês)application/pdf407558http://www.lume.ufrgs.br/bitstream/10183/212593/1/001114965.pdf40d8523fb7b1799d7550e9c1e7a64c7dMD5110183/2125932020-08-05 03:39:22.424163oai:www.lume.ufrgs.br:10183/212593Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2020-08-05T06:39:22Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Cryptococcus spp., identification and susceptibility to antifungals in isolated in laboratory of reference in The State of Rio Grande Do Sul, Brazil |
title |
Cryptococcus spp., identification and susceptibility to antifungals in isolated in laboratory of reference in The State of Rio Grande Do Sul, Brazil |
spellingShingle |
Cryptococcus spp., identification and susceptibility to antifungals in isolated in laboratory of reference in The State of Rio Grande Do Sul, Brazil Silva, Leonardo de Paulo Recuero da Farmácia Cryptococcus Cryptococcus spp. Identification Susceptibility Rio Grande do Sul |
title_short |
Cryptococcus spp., identification and susceptibility to antifungals in isolated in laboratory of reference in The State of Rio Grande Do Sul, Brazil |
title_full |
Cryptococcus spp., identification and susceptibility to antifungals in isolated in laboratory of reference in The State of Rio Grande Do Sul, Brazil |
title_fullStr |
Cryptococcus spp., identification and susceptibility to antifungals in isolated in laboratory of reference in The State of Rio Grande Do Sul, Brazil |
title_full_unstemmed |
Cryptococcus spp., identification and susceptibility to antifungals in isolated in laboratory of reference in The State of Rio Grande Do Sul, Brazil |
title_sort |
Cryptococcus spp., identification and susceptibility to antifungals in isolated in laboratory of reference in The State of Rio Grande Do Sul, Brazil |
author |
Silva, Leonardo de Paulo Recuero da |
author_facet |
Silva, Leonardo de Paulo Recuero da Garcia, Emily dos Santos Charles, Fernanda Costa Martinez, Karina de Vargas Leandro, Renata Neves Giudice, Victoria Marcon Nascimento, Caroline Gabriela Santos do Pereira, Patrícia Abreu Borges, Júlia Spier Gonçalves, Stela Maris Bottin Rodrigues, Diana Mara Garcia Picanço, João Marcelo Astolfi Calil, Luciane Noal Mezzari, Adelina |
author_role |
author |
author2 |
Garcia, Emily dos Santos Charles, Fernanda Costa Martinez, Karina de Vargas Leandro, Renata Neves Giudice, Victoria Marcon Nascimento, Caroline Gabriela Santos do Pereira, Patrícia Abreu Borges, Júlia Spier Gonçalves, Stela Maris Bottin Rodrigues, Diana Mara Garcia Picanço, João Marcelo Astolfi Calil, Luciane Noal Mezzari, Adelina |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Leonardo de Paulo Recuero da Garcia, Emily dos Santos Charles, Fernanda Costa Martinez, Karina de Vargas Leandro, Renata Neves Giudice, Victoria Marcon Nascimento, Caroline Gabriela Santos do Pereira, Patrícia Abreu Borges, Júlia Spier Gonçalves, Stela Maris Bottin Rodrigues, Diana Mara Garcia Picanço, João Marcelo Astolfi Calil, Luciane Noal Mezzari, Adelina |
dc.subject.por.fl_str_mv |
Farmácia Cryptococcus |
topic |
Farmácia Cryptococcus Cryptococcus spp. Identification Susceptibility Rio Grande do Sul |
dc.subject.eng.fl_str_mv |
Cryptococcus spp. Identification Susceptibility Rio Grande do Sul |
description |
Introduction: The Cryptococcus spp., fungus with opportunistic behavior, present in environmental sources, bird excreta, remains of trees, among others. It is inhaled by the individual, causing infection that can spread to other organs and systems with tropism through the central nervous system, causing meningoencephalitis. It is estimated that C. neoformans causes more than one million new cases of cryptococcosis per year, about 625,000 deaths. Meningoencephalitis, the most common clinical manifestation, is estimated to cause 120,000 to 240,000 deaths per year worldwide. Methods: This study was conducted from January 2012 to June 2019. Biological materials were processed, mainly the cerebrospinal fluid of patients with suspected cryptococcosis. All the phenotypically identified isolates were confirmed by the Polymerase Chain Reaction (PCR) molecular technique. The antifungals fluconazole, itraconazole and amphotericin B were also tested to verify the sensitivity profile of the isolates. Conclusions and discussion: In this study it was possible to observe and confirm a higher prevalence of Cryptococcus neoformans species than C. gattii. Azole resistance was also observed by the fungus Cryptococcus spp., suggesting a possible synergism of azoles with anti-inflammatory substances, but further studies are needed both in vitro and in vivo. The imminent biological risk from the presence of the two fungal species in the environment, including the confirmation of antifungal resistant strains, corroborates the need for continuous environmental monitoring, including the presence of new pathogenic species and consequently how to conduct treatment in the various clinic presentations by this yeast. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-08-04T03:39:12Z |
dc.date.issued.fl_str_mv |
2020 |
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Estrangeiro info:eu-repo/semantics/article |
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International journal of pharmacy and biological sciences. Índia. Vol. 10, n. 2 (Apr. 2020), p. 128-134 |
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