Bioproduction and characterization of sodium, potassium, and calcium lactobionates
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/178786 |
Resumo: | Lactobionic acid and its salts are substances that have several applications in the pharmaceutical area. These products were obtained by bioconversion through the enzymatic complex glucose-fructose-oxidoreductase (GFOR)/gluconolactonase (GL) present in calcium alginate immobilized cells of Zymomonas mobilis. In the reactions catalyzed by this enzyme system, the medium pH must be controlled at slightly acid values. For this purpose, NaOH, KOH, or Ca(OH)2 were used, and as a result, the respective salts were formed. The kinetic study on the formation of sodium, potassium and calcium lactobionates was followed by the steps of purification and characterization aiming the potential use of these compounds in the pharmaceutical area. In the assays for the bioproduction of sodium, potassium or calcium lactobionates, yields of 74, 77 and 84% were obtained, respectively. In the salts purification step, purity levels of approximately 95% were achieved. The structural identities of the lactobionate salts were determined by high resolution mass spectrometry, in addition to the 13C and 1H NMR analysis. The characterization demonstrates the selectivity of the enzymatic reaction of GFOR/GL of Z. mobilis, in the production of lactobionic acid and its salts. |
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Delagustin, Maria GabrieleGonçalves, EduardaCarra, SabrinaSilva, Thiago Barcellos daBassani, Valquiria LinckSilveira, Maurício Moura daMalvessi, Eloane2018-05-25T11:11:27Z20170100-4042http://hdl.handle.net/10183/178786001068319Lactobionic acid and its salts are substances that have several applications in the pharmaceutical area. These products were obtained by bioconversion through the enzymatic complex glucose-fructose-oxidoreductase (GFOR)/gluconolactonase (GL) present in calcium alginate immobilized cells of Zymomonas mobilis. In the reactions catalyzed by this enzyme system, the medium pH must be controlled at slightly acid values. For this purpose, NaOH, KOH, or Ca(OH)2 were used, and as a result, the respective salts were formed. The kinetic study on the formation of sodium, potassium and calcium lactobionates was followed by the steps of purification and characterization aiming the potential use of these compounds in the pharmaceutical area. In the assays for the bioproduction of sodium, potassium or calcium lactobionates, yields of 74, 77 and 84% were obtained, respectively. In the salts purification step, purity levels of approximately 95% were achieved. The structural identities of the lactobionate salts were determined by high resolution mass spectrometry, in addition to the 13C and 1H NMR analysis. The characterization demonstrates the selectivity of the enzymatic reaction of GFOR/GL of Z. mobilis, in the production of lactobionic acid and its salts.application/pdfengQuímica nova. Vol. 40, no. 9 (2017), p. 1003-1008Zymomonas mobilisFarmáciaÁcido lactobiônicoEspectrometria de massaZymomonas mobilisLactobionateLactobionic acidCharacterizationMass spectrometry13C and 1H NMR analysisBioproduction and characterization of sodium, potassium, and calcium lactobionatesinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001068319.pdf001068319.pdfTexto completo (inglês)application/pdf513764http://www.lume.ufrgs.br/bitstream/10183/178786/1/001068319.pdf0688e306e5869b06f78a1fcf47757612MD51TEXT001068319.pdf.txt001068319.pdf.txtExtracted Texttext/plain27965http://www.lume.ufrgs.br/bitstream/10183/178786/2/001068319.pdf.txt054384d776b36ca10e11a9cc716df027MD5210183/1787862019-07-25 02:32:01.262892oai:www.lume.ufrgs.br:10183/178786Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-07-25T05:32:01Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Bioproduction and characterization of sodium, potassium, and calcium lactobionates |
title |
Bioproduction and characterization of sodium, potassium, and calcium lactobionates |
spellingShingle |
Bioproduction and characterization of sodium, potassium, and calcium lactobionates Delagustin, Maria Gabriele Zymomonas mobilis Farmácia Ácido lactobiônico Espectrometria de massa Zymomonas mobilis Lactobionate Lactobionic acid Characterization Mass spectrometry 13C and 1H NMR analysis |
title_short |
Bioproduction and characterization of sodium, potassium, and calcium lactobionates |
title_full |
Bioproduction and characterization of sodium, potassium, and calcium lactobionates |
title_fullStr |
Bioproduction and characterization of sodium, potassium, and calcium lactobionates |
title_full_unstemmed |
Bioproduction and characterization of sodium, potassium, and calcium lactobionates |
title_sort |
Bioproduction and characterization of sodium, potassium, and calcium lactobionates |
author |
Delagustin, Maria Gabriele |
author_facet |
Delagustin, Maria Gabriele Gonçalves, Eduarda Carra, Sabrina Silva, Thiago Barcellos da Bassani, Valquiria Linck Silveira, Maurício Moura da Malvessi, Eloane |
author_role |
author |
author2 |
Gonçalves, Eduarda Carra, Sabrina Silva, Thiago Barcellos da Bassani, Valquiria Linck Silveira, Maurício Moura da Malvessi, Eloane |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Delagustin, Maria Gabriele Gonçalves, Eduarda Carra, Sabrina Silva, Thiago Barcellos da Bassani, Valquiria Linck Silveira, Maurício Moura da Malvessi, Eloane |
dc.subject.por.fl_str_mv |
Zymomonas mobilis Farmácia Ácido lactobiônico Espectrometria de massa |
topic |
Zymomonas mobilis Farmácia Ácido lactobiônico Espectrometria de massa Zymomonas mobilis Lactobionate Lactobionic acid Characterization Mass spectrometry 13C and 1H NMR analysis |
dc.subject.eng.fl_str_mv |
Zymomonas mobilis Lactobionate Lactobionic acid Characterization Mass spectrometry 13C and 1H NMR analysis |
description |
Lactobionic acid and its salts are substances that have several applications in the pharmaceutical area. These products were obtained by bioconversion through the enzymatic complex glucose-fructose-oxidoreductase (GFOR)/gluconolactonase (GL) present in calcium alginate immobilized cells of Zymomonas mobilis. In the reactions catalyzed by this enzyme system, the medium pH must be controlled at slightly acid values. For this purpose, NaOH, KOH, or Ca(OH)2 were used, and as a result, the respective salts were formed. The kinetic study on the formation of sodium, potassium and calcium lactobionates was followed by the steps of purification and characterization aiming the potential use of these compounds in the pharmaceutical area. In the assays for the bioproduction of sodium, potassium or calcium lactobionates, yields of 74, 77 and 84% were obtained, respectively. In the salts purification step, purity levels of approximately 95% were achieved. The structural identities of the lactobionate salts were determined by high resolution mass spectrometry, in addition to the 13C and 1H NMR analysis. The characterization demonstrates the selectivity of the enzymatic reaction of GFOR/GL of Z. mobilis, in the production of lactobionic acid and its salts. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2018-05-25T11:11:27Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
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dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/178786 |
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0100-4042 |
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001068319 |
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0100-4042 001068319 |
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http://hdl.handle.net/10183/178786 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Química nova. Vol. 40, no. 9 (2017), p. 1003-1008 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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