Comparision of lipolytic activity of Sporothrix schenckii strains utilizing olive oil-rhodamine B and tween 80
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/187479 |
Resumo: | The aim of this work was to identify the lipase activity of Sporothrix schenckii strains using Rhodamine B as an indicator of triglyceride hydrolysis (triolein from olive oil) and to compare these results with the use of Tween 80 as substrate. Free fatty acids released from lipase activity were detected on agar plate complexed with Rhodamine B, when exposed to UVA light, through orange fluorescence and by precipitation haloes on Tween 80 agar. There were differences in lipolytic activity among strains and between the media utilized. The highest lipase activity (orange fluorescence) was observed on the biomass of 10 strains, with no fluorescent haloes around the colonies, whereas only six strains showed large precipitation haloes. These plate assays are suitable for the detection of lipase-producing strains of S. schenckii in the yeast phase, but it is still unknown whether different enzymes act upon the substrates or whether there are variations of the same enzyme with different specificities. |
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Corbellini, Valeriano AntonioScroferneker, Maria LuciaCarissimi, MarianaStopiglia, Cheila Denise OttonelliSouza, Thais Furtado de2018-12-28T04:01:23Z20071415-6229http://hdl.handle.net/10183/187479000627500The aim of this work was to identify the lipase activity of Sporothrix schenckii strains using Rhodamine B as an indicator of triglyceride hydrolysis (triolein from olive oil) and to compare these results with the use of Tween 80 as substrate. Free fatty acids released from lipase activity were detected on agar plate complexed with Rhodamine B, when exposed to UVA light, through orange fluorescence and by precipitation haloes on Tween 80 agar. There were differences in lipolytic activity among strains and between the media utilized. The highest lipase activity (orange fluorescence) was observed on the biomass of 10 strains, with no fluorescent haloes around the colonies, whereas only six strains showed large precipitation haloes. These plate assays are suitable for the detection of lipase-producing strains of S. schenckii in the yeast phase, but it is still unknown whether different enzymes act upon the substrates or whether there are variations of the same enzyme with different specificities.application/pdfengTecno-lógica. Santa Cruz do Sul, RS. Vol. 11, n. 1-2 (Jan./dez. 2007), p. 33-36Rodamina BLipaseSporothrix schenckiiRhodamine BLipaseTween 80Sporothrix schenckiiComparision of lipolytic activity of Sporothrix schenckii strains utilizing olive oil-rhodamine B and tween 80info:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000627500.pdf.txt000627500.pdf.txtExtracted Texttext/plain18708http://www.lume.ufrgs.br/bitstream/10183/187479/2/000627500.pdf.txtbee1d32e6e5c5652a53f4efa66817bb4MD52ORIGINAL000627500.pdfTexto completo (inglês)application/pdf323854http://www.lume.ufrgs.br/bitstream/10183/187479/1/000627500.pdff701987f91770df95270f081de72ddb0MD5110183/1874792018-12-29 04:12:25.590593oai:www.lume.ufrgs.br:10183/187479Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-12-29T06:12:25Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Comparision of lipolytic activity of Sporothrix schenckii strains utilizing olive oil-rhodamine B and tween 80 |
title |
Comparision of lipolytic activity of Sporothrix schenckii strains utilizing olive oil-rhodamine B and tween 80 |
spellingShingle |
Comparision of lipolytic activity of Sporothrix schenckii strains utilizing olive oil-rhodamine B and tween 80 Corbellini, Valeriano Antonio Rodamina B Lipase Sporothrix schenckii Rhodamine B Lipase Tween 80 Sporothrix schenckii |
title_short |
Comparision of lipolytic activity of Sporothrix schenckii strains utilizing olive oil-rhodamine B and tween 80 |
title_full |
Comparision of lipolytic activity of Sporothrix schenckii strains utilizing olive oil-rhodamine B and tween 80 |
title_fullStr |
Comparision of lipolytic activity of Sporothrix schenckii strains utilizing olive oil-rhodamine B and tween 80 |
title_full_unstemmed |
Comparision of lipolytic activity of Sporothrix schenckii strains utilizing olive oil-rhodamine B and tween 80 |
title_sort |
Comparision of lipolytic activity of Sporothrix schenckii strains utilizing olive oil-rhodamine B and tween 80 |
author |
Corbellini, Valeriano Antonio |
author_facet |
Corbellini, Valeriano Antonio Scroferneker, Maria Lucia Carissimi, Mariana Stopiglia, Cheila Denise Ottonelli Souza, Thais Furtado de |
author_role |
author |
author2 |
Scroferneker, Maria Lucia Carissimi, Mariana Stopiglia, Cheila Denise Ottonelli Souza, Thais Furtado de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Corbellini, Valeriano Antonio Scroferneker, Maria Lucia Carissimi, Mariana Stopiglia, Cheila Denise Ottonelli Souza, Thais Furtado de |
dc.subject.por.fl_str_mv |
Rodamina B Lipase Sporothrix schenckii |
topic |
Rodamina B Lipase Sporothrix schenckii Rhodamine B Lipase Tween 80 Sporothrix schenckii |
dc.subject.eng.fl_str_mv |
Rhodamine B Lipase Tween 80 Sporothrix schenckii |
description |
The aim of this work was to identify the lipase activity of Sporothrix schenckii strains using Rhodamine B as an indicator of triglyceride hydrolysis (triolein from olive oil) and to compare these results with the use of Tween 80 as substrate. Free fatty acids released from lipase activity were detected on agar plate complexed with Rhodamine B, when exposed to UVA light, through orange fluorescence and by precipitation haloes on Tween 80 agar. There were differences in lipolytic activity among strains and between the media utilized. The highest lipase activity (orange fluorescence) was observed on the biomass of 10 strains, with no fluorescent haloes around the colonies, whereas only six strains showed large precipitation haloes. These plate assays are suitable for the detection of lipase-producing strains of S. schenckii in the yeast phase, but it is still unknown whether different enzymes act upon the substrates or whether there are variations of the same enzyme with different specificities. |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007 |
dc.date.accessioned.fl_str_mv |
2018-12-28T04:01:23Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/187479 |
dc.identifier.issn.pt_BR.fl_str_mv |
1415-6229 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000627500 |
identifier_str_mv |
1415-6229 000627500 |
url |
http://hdl.handle.net/10183/187479 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Tecno-lógica. Santa Cruz do Sul, RS. Vol. 11, n. 1-2 (Jan./dez. 2007), p. 33-36 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
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UFRGS |
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Repositório Institucional da UFRGS |
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Repositório Institucional da UFRGS |
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