First report of Rickettsia conorii in Hyalomma kumari ticks
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/259714 |
Resumo: | As a vector of wide range of pathogenic agents, ticks pose health threats to wild and domestic animals, and humans. Information is unavailable about the prevalence and spatial survey of Hyalomma kumari ticks and associated Rickettsia spp. in Pakistan. Concerning this knowledge gap, the present study aimed to molecularly detect Rickettsia species associated with H. kumari infesting small ruminants in Khyber Pakhtunkhwa (KP), Pakistan. A total of 409 H. kumari ticks were collected from 163/295 infested hosts with an infestation rate of 55.25%. A total of 204 females, 158 males, and 47 nymphs were collected. Goats were heavily infested by 224 ticks having an infestation rate of 58.33% (98/168), whereas sheep were infested by 185 ticks having a lesser infestation rate of 51.18% (65/127). Genomic DNA extracted from ticks was used for the amplification of tick (cox I, 16S rRNA, ITS-2) species and Rickettsia (gltA, ompA, and ompB) partial genes. Eighty-three ticks were subjected to PCR, and 8/83 (9.6%) were found positive for rickettsial agents. The cox I and 16S rRNA sequences of H. kumari showed 98.90–99.74% identity with H. kumari sequences reported from Pakistan, and phylogenetically clustered to the corresponding species reported from Pakistan and India. The obtained rickettsial gltA, ompA, and ompB sequences showed 100% identity with Rickettsia sp. of the Rickettsia conorii reported from Pakistan. In the phylogenetic trees, rickettsial sequences clustered with uncharacterized Rickettsia sp. from Pakistan and R. conorii from Israel, Russia, South Africa, and India. The present molecular based detection of H. kumari-associated R. conorii will facilitate effective surveillance in the region. |
id |
UFRGS-2_ecac8f88b5c21c9694bc74b458a3d11b |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/259714 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Ullah, ShafiAlouffi, AbdulazizAlmutairi, Mashal M.Islam, NabilaRehman, GauharIslam, Zia UlAhmed, HaroonVaz Junior, Itabajara da SilvaLabruna, Marcelo BahiaTanaka, TetsuyaAli, Abid2023-07-01T03:38:39Z20232076-2615http://hdl.handle.net/10183/259714001167984As a vector of wide range of pathogenic agents, ticks pose health threats to wild and domestic animals, and humans. Information is unavailable about the prevalence and spatial survey of Hyalomma kumari ticks and associated Rickettsia spp. in Pakistan. Concerning this knowledge gap, the present study aimed to molecularly detect Rickettsia species associated with H. kumari infesting small ruminants in Khyber Pakhtunkhwa (KP), Pakistan. A total of 409 H. kumari ticks were collected from 163/295 infested hosts with an infestation rate of 55.25%. A total of 204 females, 158 males, and 47 nymphs were collected. Goats were heavily infested by 224 ticks having an infestation rate of 58.33% (98/168), whereas sheep were infested by 185 ticks having a lesser infestation rate of 51.18% (65/127). Genomic DNA extracted from ticks was used for the amplification of tick (cox I, 16S rRNA, ITS-2) species and Rickettsia (gltA, ompA, and ompB) partial genes. Eighty-three ticks were subjected to PCR, and 8/83 (9.6%) were found positive for rickettsial agents. The cox I and 16S rRNA sequences of H. kumari showed 98.90–99.74% identity with H. kumari sequences reported from Pakistan, and phylogenetically clustered to the corresponding species reported from Pakistan and India. The obtained rickettsial gltA, ompA, and ompB sequences showed 100% identity with Rickettsia sp. of the Rickettsia conorii reported from Pakistan. In the phylogenetic trees, rickettsial sequences clustered with uncharacterized Rickettsia sp. from Pakistan and R. conorii from Israel, Russia, South Africa, and India. The present molecular based detection of H. kumari-associated R. conorii will facilitate effective surveillance in the region.application/pdfengAnimals. Basel. Vol. 13, no. 9 (May 2023), 1488, 18 p.RickettsiaHyalomma kumariOvinosCaprinosPaquistãoDiagnostico molecularFilogeniaIxodidaeRickettsia conoriiSmall ruminantsPakistanFirst report of Rickettsia conorii in Hyalomma kumari ticksEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001167984.pdf.txt001167984.pdf.txtExtracted Texttext/plain55605http://www.lume.ufrgs.br/bitstream/10183/259714/2/001167984.pdf.txt352e98fd3067f833ca557ca4291c4a60MD52ORIGINAL001167984.pdfTexto completo (inglês)application/pdf3386604http://www.lume.ufrgs.br/bitstream/10183/259714/1/001167984.pdf9d40de8b0b6d6754c41550fb74371ba0MD5110183/2597142023-07-02 03:41:13.352093oai:www.lume.ufrgs.br:10183/259714Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-07-02T06:41:13Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
First report of Rickettsia conorii in Hyalomma kumari ticks |
title |
First report of Rickettsia conorii in Hyalomma kumari ticks |
spellingShingle |
First report of Rickettsia conorii in Hyalomma kumari ticks Ullah, Shafi Rickettsia Hyalomma kumari Ovinos Caprinos Paquistão Diagnostico molecular Filogenia Ixodidae Rickettsia conorii Small ruminants Pakistan |
title_short |
First report of Rickettsia conorii in Hyalomma kumari ticks |
title_full |
First report of Rickettsia conorii in Hyalomma kumari ticks |
title_fullStr |
First report of Rickettsia conorii in Hyalomma kumari ticks |
title_full_unstemmed |
First report of Rickettsia conorii in Hyalomma kumari ticks |
title_sort |
First report of Rickettsia conorii in Hyalomma kumari ticks |
author |
Ullah, Shafi |
author_facet |
Ullah, Shafi Alouffi, Abdulaziz Almutairi, Mashal M. Islam, Nabila Rehman, Gauhar Islam, Zia Ul Ahmed, Haroon Vaz Junior, Itabajara da Silva Labruna, Marcelo Bahia Tanaka, Tetsuya Ali, Abid |
author_role |
author |
author2 |
Alouffi, Abdulaziz Almutairi, Mashal M. Islam, Nabila Rehman, Gauhar Islam, Zia Ul Ahmed, Haroon Vaz Junior, Itabajara da Silva Labruna, Marcelo Bahia Tanaka, Tetsuya Ali, Abid |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ullah, Shafi Alouffi, Abdulaziz Almutairi, Mashal M. Islam, Nabila Rehman, Gauhar Islam, Zia Ul Ahmed, Haroon Vaz Junior, Itabajara da Silva Labruna, Marcelo Bahia Tanaka, Tetsuya Ali, Abid |
dc.subject.por.fl_str_mv |
Rickettsia Hyalomma kumari Ovinos Caprinos Paquistão Diagnostico molecular Filogenia |
topic |
Rickettsia Hyalomma kumari Ovinos Caprinos Paquistão Diagnostico molecular Filogenia Ixodidae Rickettsia conorii Small ruminants Pakistan |
dc.subject.eng.fl_str_mv |
Ixodidae Rickettsia conorii Small ruminants Pakistan |
description |
As a vector of wide range of pathogenic agents, ticks pose health threats to wild and domestic animals, and humans. Information is unavailable about the prevalence and spatial survey of Hyalomma kumari ticks and associated Rickettsia spp. in Pakistan. Concerning this knowledge gap, the present study aimed to molecularly detect Rickettsia species associated with H. kumari infesting small ruminants in Khyber Pakhtunkhwa (KP), Pakistan. A total of 409 H. kumari ticks were collected from 163/295 infested hosts with an infestation rate of 55.25%. A total of 204 females, 158 males, and 47 nymphs were collected. Goats were heavily infested by 224 ticks having an infestation rate of 58.33% (98/168), whereas sheep were infested by 185 ticks having a lesser infestation rate of 51.18% (65/127). Genomic DNA extracted from ticks was used for the amplification of tick (cox I, 16S rRNA, ITS-2) species and Rickettsia (gltA, ompA, and ompB) partial genes. Eighty-three ticks were subjected to PCR, and 8/83 (9.6%) were found positive for rickettsial agents. The cox I and 16S rRNA sequences of H. kumari showed 98.90–99.74% identity with H. kumari sequences reported from Pakistan, and phylogenetically clustered to the corresponding species reported from Pakistan and India. The obtained rickettsial gltA, ompA, and ompB sequences showed 100% identity with Rickettsia sp. of the Rickettsia conorii reported from Pakistan. In the phylogenetic trees, rickettsial sequences clustered with uncharacterized Rickettsia sp. from Pakistan and R. conorii from Israel, Russia, South Africa, and India. The present molecular based detection of H. kumari-associated R. conorii will facilitate effective surveillance in the region. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-07-01T03:38:39Z |
dc.date.issued.fl_str_mv |
2023 |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/259714 |
dc.identifier.issn.pt_BR.fl_str_mv |
2076-2615 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001167984 |
identifier_str_mv |
2076-2615 001167984 |
url |
http://hdl.handle.net/10183/259714 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Animals. Basel. Vol. 13, no. 9 (May 2023), 1488, 18 p. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/259714/2/001167984.pdf.txt http://www.lume.ufrgs.br/bitstream/10183/259714/1/001167984.pdf |
bitstream.checksum.fl_str_mv |
352e98fd3067f833ca557ca4291c4a60 9d40de8b0b6d6754c41550fb74371ba0 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
|
_version_ |
1801225090489122816 |