Absence of A3Z3-related hypermutations in the env and vif proviral genes in FIV naturally infected cats

Detalhes bibliográficos
Autor(a) principal: Cano Ortiz, Lucía
Data de Publicação: 2018
Outros Autores: Junqueira, Dennis Maletich, Comerlato, Juliana, Zani, André Luis da Silva, Costa, Cristina Santos da, Roehe, Paulo Michel, Franco, Ana Claudia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/179798
Resumo: Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 (APOBEC3; A3) proteins comprise an important family of restriction factors that produce hypermutations on proviral DNA and are able to limit virus replication. Vif, an accessory protein present in almost all lentiviruses, counteracts the antiviral A3 activity. Seven haplotypes of APOBEC3Z3 (A3Z3) were described in domestic cats (hap I–VII), and in-vitro studies have demonstrated that these proteins reduce infectivity of vif-defective feline immunodeficiency virus (FIV). Moreover, hap V is resistant to vif-mediated degradation. However, studies on the effect of A3Z3 in FIV-infected cats have not been developed. Here, the correlation between APOBEC A3Z3 haplotypes in domestic cats and the frequency of hypermutations in the FIV vif and env genes were assessed in a retrospective cohort study with 30 blood samples collected between 2012 and 2016 from naturally FIV-infected cats in Brazil. The vif and env sequences were analyzed and displayed low or undetectable levels of hypermutations, and could not be associated with any specific A3Z3 haplotype.
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spelling Cano Ortiz, LucíaJunqueira, Dennis MaletichComerlato, JulianaZani, André Luis da SilvaCosta, Cristina Santos daRoehe, Paulo MichelFranco, Ana Claudia2018-06-27T02:34:38Z20181999-4915http://hdl.handle.net/10183/179798001070263Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 (APOBEC3; A3) proteins comprise an important family of restriction factors that produce hypermutations on proviral DNA and are able to limit virus replication. Vif, an accessory protein present in almost all lentiviruses, counteracts the antiviral A3 activity. Seven haplotypes of APOBEC3Z3 (A3Z3) were described in domestic cats (hap I–VII), and in-vitro studies have demonstrated that these proteins reduce infectivity of vif-defective feline immunodeficiency virus (FIV). Moreover, hap V is resistant to vif-mediated degradation. However, studies on the effect of A3Z3 in FIV-infected cats have not been developed. Here, the correlation between APOBEC A3Z3 haplotypes in domestic cats and the frequency of hypermutations in the FIV vif and env genes were assessed in a retrospective cohort study with 30 blood samples collected between 2012 and 2016 from naturally FIV-infected cats in Brazil. The vif and env sequences were analyzed and displayed low or undetectable levels of hypermutations, and could not be associated with any specific A3Z3 haplotype.application/pdfengViruses. Basel, Switzerland. Vol. 10, no. 6, (2018), 296Vírus da imunodeficiência felinaGatosHipermutação somática de imunoglobulinaAPOBEC3FIVHypermutationsCatsAbsence of A3Z3-related hypermutations in the env and vif proviral genes in FIV naturally infected catsEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001070263.pdf001070263.pdfTexto completo (inglês)application/pdf343244http://www.lume.ufrgs.br/bitstream/10183/179798/1/001070263.pdf7970f9f2e545e2c32e07b937db4c1b08MD51TEXT001070263.pdf.txt001070263.pdf.txtExtracted Texttext/plain25699http://www.lume.ufrgs.br/bitstream/10183/179798/2/001070263.pdf.txt967ad9ee3b728dfb5f3521cf584b1dbdMD5210183/1797982018-06-28 02:28:17.894057oai:www.lume.ufrgs.br:10183/179798Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-06-28T05:28:17Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Absence of A3Z3-related hypermutations in the env and vif proviral genes in FIV naturally infected cats
title Absence of A3Z3-related hypermutations in the env and vif proviral genes in FIV naturally infected cats
spellingShingle Absence of A3Z3-related hypermutations in the env and vif proviral genes in FIV naturally infected cats
Cano Ortiz, Lucía
Vírus da imunodeficiência felina
Gatos
Hipermutação somática de imunoglobulina
APOBEC3
FIV
Hypermutations
Cats
title_short Absence of A3Z3-related hypermutations in the env and vif proviral genes in FIV naturally infected cats
title_full Absence of A3Z3-related hypermutations in the env and vif proviral genes in FIV naturally infected cats
title_fullStr Absence of A3Z3-related hypermutations in the env and vif proviral genes in FIV naturally infected cats
title_full_unstemmed Absence of A3Z3-related hypermutations in the env and vif proviral genes in FIV naturally infected cats
title_sort Absence of A3Z3-related hypermutations in the env and vif proviral genes in FIV naturally infected cats
author Cano Ortiz, Lucía
author_facet Cano Ortiz, Lucía
Junqueira, Dennis Maletich
Comerlato, Juliana
Zani, André Luis da Silva
Costa, Cristina Santos da
Roehe, Paulo Michel
Franco, Ana Claudia
author_role author
author2 Junqueira, Dennis Maletich
Comerlato, Juliana
Zani, André Luis da Silva
Costa, Cristina Santos da
Roehe, Paulo Michel
Franco, Ana Claudia
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cano Ortiz, Lucía
Junqueira, Dennis Maletich
Comerlato, Juliana
Zani, André Luis da Silva
Costa, Cristina Santos da
Roehe, Paulo Michel
Franco, Ana Claudia
dc.subject.por.fl_str_mv Vírus da imunodeficiência felina
Gatos
Hipermutação somática de imunoglobulina
topic Vírus da imunodeficiência felina
Gatos
Hipermutação somática de imunoglobulina
APOBEC3
FIV
Hypermutations
Cats
dc.subject.eng.fl_str_mv APOBEC3
FIV
Hypermutations
Cats
description Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 (APOBEC3; A3) proteins comprise an important family of restriction factors that produce hypermutations on proviral DNA and are able to limit virus replication. Vif, an accessory protein present in almost all lentiviruses, counteracts the antiviral A3 activity. Seven haplotypes of APOBEC3Z3 (A3Z3) were described in domestic cats (hap I–VII), and in-vitro studies have demonstrated that these proteins reduce infectivity of vif-defective feline immunodeficiency virus (FIV). Moreover, hap V is resistant to vif-mediated degradation. However, studies on the effect of A3Z3 in FIV-infected cats have not been developed. Here, the correlation between APOBEC A3Z3 haplotypes in domestic cats and the frequency of hypermutations in the FIV vif and env genes were assessed in a retrospective cohort study with 30 blood samples collected between 2012 and 2016 from naturally FIV-infected cats in Brazil. The vif and env sequences were analyzed and displayed low or undetectable levels of hypermutations, and could not be associated with any specific A3Z3 haplotype.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-06-27T02:34:38Z
dc.date.issued.fl_str_mv 2018
dc.type.driver.fl_str_mv Estrangeiro
info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/179798
dc.identifier.issn.pt_BR.fl_str_mv 1999-4915
dc.identifier.nrb.pt_BR.fl_str_mv 001070263
identifier_str_mv 1999-4915
001070263
url http://hdl.handle.net/10183/179798
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Viruses. Basel, Switzerland. Vol. 10, no. 6, (2018), 296
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRGS
instname:Universidade Federal do Rio Grande do Sul (UFRGS)
instacron:UFRGS
instname_str Universidade Federal do Rio Grande do Sul (UFRGS)
instacron_str UFRGS
institution UFRGS
reponame_str Repositório Institucional da UFRGS
collection Repositório Institucional da UFRGS
bitstream.url.fl_str_mv http://www.lume.ufrgs.br/bitstream/10183/179798/1/001070263.pdf
http://www.lume.ufrgs.br/bitstream/10183/179798/2/001070263.pdf.txt
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