Lipase production by Aspergillus brasiliensis in solid-state cultivation of malt bagasse in different bioreactors configurations
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/263906 |
Resumo: | We evaluated the conditions to produce lipase in solid-state cultivation using a recently isolated strain of Aspergillus brasiliensis 157f in bioreactors of different confi gurations: static fl at-bed, plugged-fl ow bed with forced water-saturated aeration, and pilot-scale rotating drum bioreactor, using malt bagasse as substrate. Lipase production was optimized applying experimental design analysis, which showed optima parameters defi ned as pH 7.7, addition of 11.3 % of soybean oil to the medium, and culture temperature of 32.7 o C, in static fl at-bed. The highest enzyme activity (9.8 U.g-1 substrate) was obtained in the plugged-fl ow bed with forced water-saturated aeration. The fermented culture medium was lyophilized to create a solid enzymatic preparation (SEP), which was used to test the possibility of using this cheap biocatalyst in bioreactors to mediate esterifi cation and transesterifi cation reactions. SEP presented lipase activities of 7.35 U.g-1 substrate, indicating the possibility of further enhancing aspects of the use of such biocatalyst. |
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Eichler, PauloBastiani, Daniela deSantos, Fernando AlmeidaAyub, Marco Antônio Záchia2023-08-23T03:30:23Z20200001-3765http://hdl.handle.net/10183/263906001174150We evaluated the conditions to produce lipase in solid-state cultivation using a recently isolated strain of Aspergillus brasiliensis 157f in bioreactors of different confi gurations: static fl at-bed, plugged-fl ow bed with forced water-saturated aeration, and pilot-scale rotating drum bioreactor, using malt bagasse as substrate. Lipase production was optimized applying experimental design analysis, which showed optima parameters defi ned as pH 7.7, addition of 11.3 % of soybean oil to the medium, and culture temperature of 32.7 o C, in static fl at-bed. The highest enzyme activity (9.8 U.g-1 substrate) was obtained in the plugged-fl ow bed with forced water-saturated aeration. The fermented culture medium was lyophilized to create a solid enzymatic preparation (SEP), which was used to test the possibility of using this cheap biocatalyst in bioreactors to mediate esterifi cation and transesterifi cation reactions. SEP presented lipase activities of 7.35 U.g-1 substrate, indicating the possibility of further enhancing aspects of the use of such biocatalyst.application/pdfengAnais da Academia Brasileira de Ciências. Rio de Janeiro. Vol. 92, suppl. 2 (2020), e20180856, 15 p.AspergillusLipaseMalteTransesterificaçãoAspergillus brasiliensisBrewery’s spent maltLipase productionSolid-state cultivationTransesterification reactionLipase production by Aspergillus brasiliensis in solid-state cultivation of malt bagasse in different bioreactors configurationsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001174150.pdf.txt001174150.pdf.txtExtracted Texttext/plain0http://www.lume.ufrgs.br/bitstream/10183/263906/2/001174150.pdf.txtd41d8cd98f00b204e9800998ecf8427eMD52ORIGINAL001174150.pdfTexto completo (inglês)application/pdf3224880http://www.lume.ufrgs.br/bitstream/10183/263906/1/001174150.pdf7e4d2e7a3f0d3e7eff5b9c164148fa2dMD5110183/2639062023-08-24 03:34:34.088689oai:www.lume.ufrgs.br:10183/263906Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-08-24T06:34:34Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Lipase production by Aspergillus brasiliensis in solid-state cultivation of malt bagasse in different bioreactors configurations |
title |
Lipase production by Aspergillus brasiliensis in solid-state cultivation of malt bagasse in different bioreactors configurations |
spellingShingle |
Lipase production by Aspergillus brasiliensis in solid-state cultivation of malt bagasse in different bioreactors configurations Eichler, Paulo Aspergillus Lipase Malte Transesterificação Aspergillus brasiliensis Brewery’s spent malt Lipase production Solid-state cultivation Transesterification reaction |
title_short |
Lipase production by Aspergillus brasiliensis in solid-state cultivation of malt bagasse in different bioreactors configurations |
title_full |
Lipase production by Aspergillus brasiliensis in solid-state cultivation of malt bagasse in different bioreactors configurations |
title_fullStr |
Lipase production by Aspergillus brasiliensis in solid-state cultivation of malt bagasse in different bioreactors configurations |
title_full_unstemmed |
Lipase production by Aspergillus brasiliensis in solid-state cultivation of malt bagasse in different bioreactors configurations |
title_sort |
Lipase production by Aspergillus brasiliensis in solid-state cultivation of malt bagasse in different bioreactors configurations |
author |
Eichler, Paulo |
author_facet |
Eichler, Paulo Bastiani, Daniela de Santos, Fernando Almeida Ayub, Marco Antônio Záchia |
author_role |
author |
author2 |
Bastiani, Daniela de Santos, Fernando Almeida Ayub, Marco Antônio Záchia |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Eichler, Paulo Bastiani, Daniela de Santos, Fernando Almeida Ayub, Marco Antônio Záchia |
dc.subject.por.fl_str_mv |
Aspergillus Lipase Malte Transesterificação |
topic |
Aspergillus Lipase Malte Transesterificação Aspergillus brasiliensis Brewery’s spent malt Lipase production Solid-state cultivation Transesterification reaction |
dc.subject.eng.fl_str_mv |
Aspergillus brasiliensis Brewery’s spent malt Lipase production Solid-state cultivation Transesterification reaction |
description |
We evaluated the conditions to produce lipase in solid-state cultivation using a recently isolated strain of Aspergillus brasiliensis 157f in bioreactors of different confi gurations: static fl at-bed, plugged-fl ow bed with forced water-saturated aeration, and pilot-scale rotating drum bioreactor, using malt bagasse as substrate. Lipase production was optimized applying experimental design analysis, which showed optima parameters defi ned as pH 7.7, addition of 11.3 % of soybean oil to the medium, and culture temperature of 32.7 o C, in static fl at-bed. The highest enzyme activity (9.8 U.g-1 substrate) was obtained in the plugged-fl ow bed with forced water-saturated aeration. The fermented culture medium was lyophilized to create a solid enzymatic preparation (SEP), which was used to test the possibility of using this cheap biocatalyst in bioreactors to mediate esterifi cation and transesterifi cation reactions. SEP presented lipase activities of 7.35 U.g-1 substrate, indicating the possibility of further enhancing aspects of the use of such biocatalyst. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020 |
dc.date.accessioned.fl_str_mv |
2023-08-23T03:30:23Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/263906 |
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0001-3765 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001174150 |
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0001-3765 001174150 |
url |
http://hdl.handle.net/10183/263906 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Anais da Academia Brasileira de Ciências. Rio de Janeiro. Vol. 92, suppl. 2 (2020), e20180856, 15 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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