Influence of caries activity and number of saliva donors : mineral and microbiological responses in a microcosm biofilm model

• Autor(a) principal: Viana, Chayane De Souza
• Data de Publicação: 2021
• Outros Autores: Maske, Tamires Timm, Signori, Cácia, Leite, Françoise Hélène van de Sande, Oliveira, Elenara Ferreira de, Cenci, Maximiliano Sérgio
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/239861
• topic: Biofilmes; Cárie dentária; Desmineralização do dente; Saliva; Biofilm; Microcosm; Dental caries; Demineralization; Saliva; Caries activity

Objective: this study evaluated the mineral and microbiological response of biofilms originating from different types of saliva inoculum with distinct levels of caries activity. Methodology: the biofilms grown over enamel specimens originated from saliva collected from a single donor or five donors with two distinct levels of caries activity (caries-active and caries-free) or from pooling saliva from ten donors (five caries-active and five caries-free). The percentage surface hardness change (%SHC) and microbiological counts served as outcome variables. Results: the caries activity of donors did not affect the %SHC values. Inoculum from five donors compared to a single donor showed higher %SHC values (p=0.019). Higher lactobacilli counts were observed when saliva from caries-active donors was used as the inoculum (p=0.017). Pooled saliva from both caries activity levels showed higher mutans streptococci counts (p<0.017). Conclusion: Overall, pooled saliva increased the mineral response of the derived biofilms, but all the inoculum conditions formed cariogenic biofilms and caries lesions independently of caries activity.