Fusarium solani keratitis : role of antifungal susceptibility testing and identification to the species level for proper management

Detalhes bibliográficos
Autor(a) principal: Rosa, Priscila Dallé da
Data de Publicação: 2019
Outros Autores: Scheid, Karla Linck, Locatelli, Claudete Inês, Marinho, Diane Ruschel, Goldani, Luciano Zubaran
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/216333
Resumo: We report a patient with fungal keratitis caused by a multiresistant Fusarium solani in a tertiary care hospital located in southern Brazil. A 55-year-old man with a history of ocular trauma presented with keratitis in left eye. The patient has a complicated clinical course and failed to respond to local and systemic antifungal treatment, and required eye enucleation. Despite multiple topical, intraocular and systemic antifungal treatments, hyphal infiltration persisted in the corneal transplant causing continuous recurrences. The cultures of corneal biopsy scrapings were positive for Fusarium spp. The organism was identified to species level by multi-locus sequencing for translation elongation factor 1 alpha (EF-1), and RNA polymerase II subunit (RPB2). In vitro antifungal susceptibility testing of the isolate by the broth microdilution method, according to CLSI M38-A2, disclosed susceptibility to natamycin and resistance to amphotericin B, voriconazole, itraconazole and fluconazole. Considering previous unsuccessful antifungal treatments due to multiple drug resistance, the eye was enucleated. Our case report illustrates that management of fungal keratitis remains a therapeutic challenge. Optimal treatment for F. solani infection has not yet been established and should include susceptibility testing for different antifungal agents.
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spelling Rosa, Priscila Dallé daScheid, Karla LinckLocatelli, Claudete InêsMarinho, Diane RuschelGoldani, Luciano Zubaran2020-12-11T04:11:13Z20191413-8670http://hdl.handle.net/10183/216333001119241We report a patient with fungal keratitis caused by a multiresistant Fusarium solani in a tertiary care hospital located in southern Brazil. A 55-year-old man with a history of ocular trauma presented with keratitis in left eye. The patient has a complicated clinical course and failed to respond to local and systemic antifungal treatment, and required eye enucleation. Despite multiple topical, intraocular and systemic antifungal treatments, hyphal infiltration persisted in the corneal transplant causing continuous recurrences. The cultures of corneal biopsy scrapings were positive for Fusarium spp. The organism was identified to species level by multi-locus sequencing for translation elongation factor 1 alpha (EF-1), and RNA polymerase II subunit (RPB2). In vitro antifungal susceptibility testing of the isolate by the broth microdilution method, according to CLSI M38-A2, disclosed susceptibility to natamycin and resistance to amphotericin B, voriconazole, itraconazole and fluconazole. Considering previous unsuccessful antifungal treatments due to multiple drug resistance, the eye was enucleated. Our case report illustrates that management of fungal keratitis remains a therapeutic challenge. Optimal treatment for F. solani infection has not yet been established and should include susceptibility testing for different antifungal agents.application/pdfengThe Brazilian journal of infectious diseases. Vol. 23, no. 3 (2019), p. 197-199Úlcera da córneaCeratiteFusariumFarmacorresistência fúngicaKeratitisCorneal ulcerFusarium solaniAntifungal agents resistanceFusarium solani keratitis : role of antifungal susceptibility testing and identification to the species level for proper managementinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001119241.pdf.txt001119241.pdf.txtExtracted Texttext/plain9785http://www.lume.ufrgs.br/bitstream/10183/216333/2/001119241.pdf.txt4b45697fa4a4d5c4e32f9c148e312406MD52ORIGINAL001119241.pdfTexto completo (inglês)application/pdf695455http://www.lume.ufrgs.br/bitstream/10183/216333/1/001119241.pdf501d0d44e0adcbec4ffb49bad900b0c1MD5110183/2163332021-05-07 04:35:12.543518oai:www.lume.ufrgs.br:10183/216333Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-05-07T07:35:12Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Fusarium solani keratitis : role of antifungal susceptibility testing and identification to the species level for proper management
title Fusarium solani keratitis : role of antifungal susceptibility testing and identification to the species level for proper management
spellingShingle Fusarium solani keratitis : role of antifungal susceptibility testing and identification to the species level for proper management
Rosa, Priscila Dallé da
Úlcera da córnea
Ceratite
Fusarium
Farmacorresistência fúngica
Keratitis
Corneal ulcer
Fusarium solani
Antifungal agents resistance
title_short Fusarium solani keratitis : role of antifungal susceptibility testing and identification to the species level for proper management
title_full Fusarium solani keratitis : role of antifungal susceptibility testing and identification to the species level for proper management
title_fullStr Fusarium solani keratitis : role of antifungal susceptibility testing and identification to the species level for proper management
title_full_unstemmed Fusarium solani keratitis : role of antifungal susceptibility testing and identification to the species level for proper management
title_sort Fusarium solani keratitis : role of antifungal susceptibility testing and identification to the species level for proper management
author Rosa, Priscila Dallé da
author_facet Rosa, Priscila Dallé da
Scheid, Karla Linck
Locatelli, Claudete Inês
Marinho, Diane Ruschel
Goldani, Luciano Zubaran
author_role author
author2 Scheid, Karla Linck
Locatelli, Claudete Inês
Marinho, Diane Ruschel
Goldani, Luciano Zubaran
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Rosa, Priscila Dallé da
Scheid, Karla Linck
Locatelli, Claudete Inês
Marinho, Diane Ruschel
Goldani, Luciano Zubaran
dc.subject.por.fl_str_mv Úlcera da córnea
Ceratite
Fusarium
Farmacorresistência fúngica
topic Úlcera da córnea
Ceratite
Fusarium
Farmacorresistência fúngica
Keratitis
Corneal ulcer
Fusarium solani
Antifungal agents resistance
dc.subject.eng.fl_str_mv Keratitis
Corneal ulcer
Fusarium solani
Antifungal agents resistance
description We report a patient with fungal keratitis caused by a multiresistant Fusarium solani in a tertiary care hospital located in southern Brazil. A 55-year-old man with a history of ocular trauma presented with keratitis in left eye. The patient has a complicated clinical course and failed to respond to local and systemic antifungal treatment, and required eye enucleation. Despite multiple topical, intraocular and systemic antifungal treatments, hyphal infiltration persisted in the corneal transplant causing continuous recurrences. The cultures of corneal biopsy scrapings were positive for Fusarium spp. The organism was identified to species level by multi-locus sequencing for translation elongation factor 1 alpha (EF-1), and RNA polymerase II subunit (RPB2). In vitro antifungal susceptibility testing of the isolate by the broth microdilution method, according to CLSI M38-A2, disclosed susceptibility to natamycin and resistance to amphotericin B, voriconazole, itraconazole and fluconazole. Considering previous unsuccessful antifungal treatments due to multiple drug resistance, the eye was enucleated. Our case report illustrates that management of fungal keratitis remains a therapeutic challenge. Optimal treatment for F. solani infection has not yet been established and should include susceptibility testing for different antifungal agents.
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2020-12-11T04:11:13Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/216333
dc.identifier.issn.pt_BR.fl_str_mv 1413-8670
dc.identifier.nrb.pt_BR.fl_str_mv 001119241
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv The Brazilian journal of infectious diseases. Vol. 23, no. 3 (2019), p. 197-199
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reponame_str Repositório Institucional da UFRGS
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