Morphological and Molecular Identification of Entomopathogenic Fungi from Agricultural and Forestry Crops

Detalhes bibliográficos
Autor(a) principal: Bich,Gustavo Angel
Data de Publicação: 2021
Outros Autores: Castrillo,María Lorena, Kramer,Fernando Luis, Villalba,Laura Lidia, Zapata,Pedro Darío
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Floresta e Ambiente
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2179-80872021000200311
Resumo: Abstract A promising alternative for insect pest control in agriculture is the use of entomopathogenic fungi. The objectives of this work were to isolate and identify morphologically and molecularly strains of entomopathogenic fungi from crops of agricultural and forestry interest for the development of insect biocontrol technologies in Argentina. Three strains of Beauveria bassiana, and two strains of Metarhizium anisopliae were isolated and morphologically identified. Identity values greater than 99% with strains of B. bassiana and M. anisopliae were obtained in molecular databases. It was also confirmed by phylogenetic methods that the sequences obtained from these strains were positioned within the clade that contained the sequences of reference of B. bassiana and M. anisopliae respectively. These results deepen the knowledge of the mycobiota of Argentinian crops as a basis for the development of biotechnological products in the area of biological control.
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spelling Morphological and Molecular Identification of Entomopathogenic Fungi from Agricultural and Forestry CropsBeauveriaMetarhiziumMicroscopyrDNAPhylogenyAbstract A promising alternative for insect pest control in agriculture is the use of entomopathogenic fungi. The objectives of this work were to isolate and identify morphologically and molecularly strains of entomopathogenic fungi from crops of agricultural and forestry interest for the development of insect biocontrol technologies in Argentina. Three strains of Beauveria bassiana, and two strains of Metarhizium anisopliae were isolated and morphologically identified. Identity values greater than 99% with strains of B. bassiana and M. anisopliae were obtained in molecular databases. It was also confirmed by phylogenetic methods that the sequences obtained from these strains were positioned within the clade that contained the sequences of reference of B. bassiana and M. anisopliae respectively. These results deepen the knowledge of the mycobiota of Argentinian crops as a basis for the development of biotechnological products in the area of biological control.Instituto de Florestas da Universidade Federal Rural do Rio de Janeiro2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2179-80872021000200311Floresta e Ambiente v.28 n.2 2021reponame:Floresta e Ambienteinstname:Universidade Federal do Rio de Janeiro (UFRJ)instacron:UFRJ10.1590/2179-8087-floram-2018-0086info:eu-repo/semantics/openAccessBich,Gustavo AngelCastrillo,María LorenaKramer,Fernando LuisVillalba,Laura LidiaZapata,Pedro Daríoeng2021-01-18T00:00:00Zoai:scielo:S2179-80872021000200311Revistahttps://www.floram.org/PUBhttps://old.scielo.br/oai/scielo-oai.phpfloramjournal@gmail.com||floram@ufrrj.br||2179-80871415-0980opendoar:2021-01-18T00:00Floresta e Ambiente - Universidade Federal do Rio de Janeiro (UFRJ)false
dc.title.none.fl_str_mv Morphological and Molecular Identification of Entomopathogenic Fungi from Agricultural and Forestry Crops
title Morphological and Molecular Identification of Entomopathogenic Fungi from Agricultural and Forestry Crops
spellingShingle Morphological and Molecular Identification of Entomopathogenic Fungi from Agricultural and Forestry Crops
Bich,Gustavo Angel
Beauveria
Metarhizium
Microscopy
rDNA
Phylogeny
title_short Morphological and Molecular Identification of Entomopathogenic Fungi from Agricultural and Forestry Crops
title_full Morphological and Molecular Identification of Entomopathogenic Fungi from Agricultural and Forestry Crops
title_fullStr Morphological and Molecular Identification of Entomopathogenic Fungi from Agricultural and Forestry Crops
title_full_unstemmed Morphological and Molecular Identification of Entomopathogenic Fungi from Agricultural and Forestry Crops
title_sort Morphological and Molecular Identification of Entomopathogenic Fungi from Agricultural and Forestry Crops
author Bich,Gustavo Angel
author_facet Bich,Gustavo Angel
Castrillo,María Lorena
Kramer,Fernando Luis
Villalba,Laura Lidia
Zapata,Pedro Darío
author_role author
author2 Castrillo,María Lorena
Kramer,Fernando Luis
Villalba,Laura Lidia
Zapata,Pedro Darío
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Bich,Gustavo Angel
Castrillo,María Lorena
Kramer,Fernando Luis
Villalba,Laura Lidia
Zapata,Pedro Darío
dc.subject.por.fl_str_mv Beauveria
Metarhizium
Microscopy
rDNA
Phylogeny
topic Beauveria
Metarhizium
Microscopy
rDNA
Phylogeny
description Abstract A promising alternative for insect pest control in agriculture is the use of entomopathogenic fungi. The objectives of this work were to isolate and identify morphologically and molecularly strains of entomopathogenic fungi from crops of agricultural and forestry interest for the development of insect biocontrol technologies in Argentina. Three strains of Beauveria bassiana, and two strains of Metarhizium anisopliae were isolated and morphologically identified. Identity values greater than 99% with strains of B. bassiana and M. anisopliae were obtained in molecular databases. It was also confirmed by phylogenetic methods that the sequences obtained from these strains were positioned within the clade that contained the sequences of reference of B. bassiana and M. anisopliae respectively. These results deepen the knowledge of the mycobiota of Argentinian crops as a basis for the development of biotechnological products in the area of biological control.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2179-80872021000200311
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2179-80872021000200311
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/2179-8087-floram-2018-0086
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Florestas da Universidade Federal Rural do Rio de Janeiro
publisher.none.fl_str_mv Instituto de Florestas da Universidade Federal Rural do Rio de Janeiro
dc.source.none.fl_str_mv Floresta e Ambiente v.28 n.2 2021
reponame:Floresta e Ambiente
instname:Universidade Federal do Rio de Janeiro (UFRJ)
instacron:UFRJ
instname_str Universidade Federal do Rio de Janeiro (UFRJ)
instacron_str UFRJ
institution UFRJ
reponame_str Floresta e Ambiente
collection Floresta e Ambiente
repository.name.fl_str_mv Floresta e Ambiente - Universidade Federal do Rio de Janeiro (UFRJ)
repository.mail.fl_str_mv floramjournal@gmail.com||floram@ufrrj.br||
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