Phylogenetic analysis and identification of a cellulase producing Bacillus Sp. Strain by 16s RRNA sequencing

• Autor(a) principal: Santos, Yago Queiroz dos
• Data de Publicação: 2019
• Outros Autores: França, Anderson Felipe Jácome de, Veras, Bruno Oliveira de, Carelli, Gabriella Silva Campos, Moura, Geovanna Maria Medeiros, Cruz, Joelton Igor Oliveira da, Oliveira, Fernanda Granja da Silva, Oliveira, João Ricardhis Saturnino de, Amorim, Luciclaudio Cassimiro de, Santos, Elizeu Antunes dos
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRN
• Texto Completo: https://repositorio.ufrn.br/handle/123456789/32805
• topic: Endoglucanase; Marine bacteria; rRNA

The microorganisms belonging to genus Bacillus include a wide spectra and ubiquitous group of bacteria that can be found from the forest soil, to marine ecosystems occurring in association with a variety of aquatic organisms such as scleractinian corals present in the intertidal boulders. Therefore, these microorganisms are exposed to various abiotic stresses that cause ecological selection for a physiologically adapted microbiota to such extremes of temperature and salinity. In this work, we isolated and characterized a cellulase from a marine bacterial strain and indentified its specie by 16S rRNA sequencing followed by a BLAST analysis. The cellulolytic strain called SR22 showed to be a gram-positive spore-forming bacilli, facultative anaerobe, and catalase positive, as well as negative for indole, H2S production, and citrate utilization; those findings led us to consider the isolate belonging to the genus Bacillus, which was confirmed by the phylogenetic analysis, which revealed that the SR22 strain formed a clade with Bacillus subtilis. Its nucleotide sequence was deposited in GenBank as Accession No. MH119099 and the degree of sequence similarity of strain SR22 to Bacillus sp. was 99%. Taken together, the present data indicate the present celulase-positive indentified strain as a potential and useful candidate for industrial applications that employs celulase degrading processes like second-generation bioethanol and paper industries being still necessary further studies to complete characterize this microorganism secretome