Operational intensification by direct product sequestration from cell disruptates: application of a pellicular adsorbent in a mechanically integrated disruption-fluidised bed adsorption process

• Autor(a) principal: Santos, Everaldo Silvino dos
• Data de Publicação: 2002
• Outros Autores: Jahanshahi, Mohsen, Sun, Yan, Pacek, Andrzej, Franco, Telma Teixera, Nienow, Alvin, Lyddiatt, Andrew
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRN
• Texto Completo: https://repositorio.ufrn.br/handle/123456789/32675
• topic: Pellicular adsorbents; Fluidised bed adsorption; Process intensification; Process integration

A novel prototype adsorbent, designed for in-tensi®ed ¯uidised bed adsorption processes, was as-sembled by the emulsi®cation coating of 4% (w/v)porous agarose upon a zirconia-silica solid core. Theadsorbent, designated ZSA (particle density 1.75 g/ml,maximum pellicle depth 40 lm), was subjected tophysical and biochemical comparison with the perfor-mance of two commercial adsorbents (Streamline andMacrosorb K4AX). Bed expansion qualities and hydro-dynamic characteristics (N, Daxland Bo) of ZSA demon-strated a marked robustness in the face of elevatedvelocities (up to 550 cm/h) and biomass loading (up to30% (ww/v)) disrupted yeast cells. Cibracron Blue deriv-atives of the pellicular prototype (ZSA-CB), evaluated inthe batch and ¯uidised bed recovery of glyceraldehyde3-phosphate dehydrogenase (G3PDH) from unclari®edyeast disruptates, exhibited superior capacities and ad-sorption/desorption performance to the commercial derivatives. These advanced physical and biochemicalproperties facilitated a demonstration of the direct, mechanical coupling of bead-milling and ¯uidised bed ad-sorption in a fully integrated process for the acceleratedrecovery of G3PDH from yeast. The generic applicationof such pellicular adsorbents and integrated processesto the recovery of labile, intracellular products is dis-cussed