Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats

Detalhes bibliográficos
Autor(a) principal: Dantas, Anna Carolina Batista
Data de Publicação: 2010
Outros Autores: Batista Júnior, Francisco Fábio de Araújo, Macedo, Larissa Freitas, Mendes, Mariana Noronha Castro, Azevedo, Ítalo Medeiros, Medeiros, Aldo Cunha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/jspui/handle/1/6283
Resumo: Cyclophosphamide (CYP) is an antineoplastic agent used for the treatment of many neoplastic and inflammatory diseases. Hemorrhagic cystitis is a frequent side effect of CYP. Several studies show that simvastatin has important pleiotropic (anti-inflammatory and immunomodulatory) effects. The purpose of the study was to investigate the effect of simvastatin on bladder, ureter and kidney injury caused by CYP. Methods: Adult male Wistar rats were randomly divided into three groups. The CYP/SIM group received simvastatin microemulsion by gavage during 7 days (10 mg/kg body wt) before the administration of CYP and the CYP/SAL group rats received saline 0.9%. The control rats were not treated. After that, all rats were treated with a single dose of CYP 200 mg/kg body wt intraperitoneally. The rats were killed 24 h after CYP administration. Plasma cytokines (TNF-a, IL-1b, IL-6) were measured by ELISA. Macro and light microscopic study was performed in the bladder, kidney and ureter. Results: In the bladders of CYP/SIMV treated rats edema of lamina propria with epithelial and sub-epithelial hemorrhage were lower than in CYP/SAL treated rats. The scores for macroscopic and microscopic evaluation of bladder and ureter were significantly lower in CYP/SIMV rats than in CYP/SAL rats. The kidney was not affected. The expression of TNF-a, IL-1b and IL-6 was significatly lower in CF/SINV rats (164.8±22, 44.8±8 and 52.4±13) than in CF/SAL rats (378.5±66, 122.9±26 e 123.6±18), respectively. Conclusion: The results of the current study suggest that simvastatin pretreatment attenuated CYP-induced urotelium inflammation and decreased the activities of cytokines
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spelling Dantas, Anna Carolina BatistaBatista Júnior, Francisco Fábio de AraújoMacedo, Larissa FreitasMendes, Mariana Noronha CastroAzevedo, Ítalo MedeirosMedeiros, Aldo Cunha2013-09-26T14:07:53Z2013-09-26T14:07:53Z2010DANTAS, Anna Carolina Batista ; BATISTA JÚNIOR, Francisco Fábio de Araújo ; MACEDO, Larissa Freitas ; MENDES, Mariana Noronha Castro ; AZEVEDO, Ítalo Medeiros ; MEDEIROS, Aldo Cunha . Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats. Acta Cirurgica Brasileira (Impresso) , v. 25, p. 43-46, 2010. Disponível em:<http://www.scielo.br/scielo.php?pid=S0102-86502010000100011&script=sci_arttext> Acesso em: 25 set. 2013.1678-2674https://repositorio.ufrn.br/jspui/handle/1/6283Cyclophosphamide (CYP) is an antineoplastic agent used for the treatment of many neoplastic and inflammatory diseases. Hemorrhagic cystitis is a frequent side effect of CYP. Several studies show that simvastatin has important pleiotropic (anti-inflammatory and immunomodulatory) effects. The purpose of the study was to investigate the effect of simvastatin on bladder, ureter and kidney injury caused by CYP. Methods: Adult male Wistar rats were randomly divided into three groups. The CYP/SIM group received simvastatin microemulsion by gavage during 7 days (10 mg/kg body wt) before the administration of CYP and the CYP/SAL group rats received saline 0.9%. The control rats were not treated. After that, all rats were treated with a single dose of CYP 200 mg/kg body wt intraperitoneally. The rats were killed 24 h after CYP administration. Plasma cytokines (TNF-a, IL-1b, IL-6) were measured by ELISA. Macro and light microscopic study was performed in the bladder, kidney and ureter. Results: In the bladders of CYP/SIMV treated rats edema of lamina propria with epithelial and sub-epithelial hemorrhage were lower than in CYP/SAL treated rats. The scores for macroscopic and microscopic evaluation of bladder and ureter were significantly lower in CYP/SIMV rats than in CYP/SAL rats. The kidney was not affected. The expression of TNF-a, IL-1b and IL-6 was significatly lower in CF/SINV rats (164.8±22, 44.8±8 and 52.4±13) than in CF/SAL rats (378.5±66, 122.9±26 e 123.6±18), respectively. Conclusion: The results of the current study suggest that simvastatin pretreatment attenuated CYP-induced urotelium inflammation and decreased the activities of cytokinesengCyclophosphamideCystitisSimvastatinInflammationCytokineRatsProtective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in ratsEfeito protetor da sinvastatina na cistite hemorrágica induzida pela ciclofosfamida em ratosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-823898https://repositorio.ufrn.br/bitstream/1/6283/2/license_rdfe363e809996cf46ada20da1accfcd9c7MD52license_textlicense_texttext/html; charset=utf-80https://repositorio.ufrn.br/bitstream/1/6283/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_urllicense_urltext/plain; charset=utf-852https://repositorio.ufrn.br/bitstream/1/6283/4/license_url3d480ae6c91e310daba2020f8787d6f9MD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufrn.br/bitstream/1/6283/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD55ORIGINALProtectiveEffectSimvastatin_Dantas_2010.pdfProtectiveEffectSimvastatin_Dantas_2010.pdfapplication/pdf2277529https://repositorio.ufrn.br/bitstream/1/6283/1/ProtectiveEffectSimvastatin_Dantas_2010.pdf8d2334a546510c5a76529dc8428c8686MD51TEXTAldoCM_Protecttive_11.pdf.txtAldoCM_Protecttive_11.pdf.txtExtracted texttext/plain22878https://repositorio.ufrn.br/bitstream/1/6283/10/AldoCM_Protecttive_11.pdf.txtb4c33755b6520d59c81353bff511328bMD510THUMBNAILAldoCM_Protecttive_11.pdf.jpgAldoCM_Protecttive_11.pdf.jpgIM Thumbnailimage/jpeg11383https://repositorio.ufrn.br/bitstream/1/6283/11/AldoCM_Protecttive_11.pdf.jpgffe1553922d907799e0581a89f8d91d9MD5111/62832021-11-30 17:55:55.173oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-11-30T20:55:55Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats
dc.title.alternative.pt_BR.fl_str_mv Efeito protetor da sinvastatina na cistite hemorrágica induzida pela ciclofosfamida em ratos
title Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats
spellingShingle Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats
Dantas, Anna Carolina Batista
Cyclophosphamide
Cystitis
Simvastatin
Inflammation
Cytokine
Rats
title_short Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats
title_full Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats
title_fullStr Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats
title_full_unstemmed Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats
title_sort Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats
author Dantas, Anna Carolina Batista
author_facet Dantas, Anna Carolina Batista
Batista Júnior, Francisco Fábio de Araújo
Macedo, Larissa Freitas
Mendes, Mariana Noronha Castro
Azevedo, Ítalo Medeiros
Medeiros, Aldo Cunha
author_role author
author2 Batista Júnior, Francisco Fábio de Araújo
Macedo, Larissa Freitas
Mendes, Mariana Noronha Castro
Azevedo, Ítalo Medeiros
Medeiros, Aldo Cunha
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Dantas, Anna Carolina Batista
Batista Júnior, Francisco Fábio de Araújo
Macedo, Larissa Freitas
Mendes, Mariana Noronha Castro
Azevedo, Ítalo Medeiros
Medeiros, Aldo Cunha
dc.subject.por.fl_str_mv Cyclophosphamide
Cystitis
Simvastatin
Inflammation
Cytokine
Rats
topic Cyclophosphamide
Cystitis
Simvastatin
Inflammation
Cytokine
Rats
description Cyclophosphamide (CYP) is an antineoplastic agent used for the treatment of many neoplastic and inflammatory diseases. Hemorrhagic cystitis is a frequent side effect of CYP. Several studies show that simvastatin has important pleiotropic (anti-inflammatory and immunomodulatory) effects. The purpose of the study was to investigate the effect of simvastatin on bladder, ureter and kidney injury caused by CYP. Methods: Adult male Wistar rats were randomly divided into three groups. The CYP/SIM group received simvastatin microemulsion by gavage during 7 days (10 mg/kg body wt) before the administration of CYP and the CYP/SAL group rats received saline 0.9%. The control rats were not treated. After that, all rats were treated with a single dose of CYP 200 mg/kg body wt intraperitoneally. The rats were killed 24 h after CYP administration. Plasma cytokines (TNF-a, IL-1b, IL-6) were measured by ELISA. Macro and light microscopic study was performed in the bladder, kidney and ureter. Results: In the bladders of CYP/SIMV treated rats edema of lamina propria with epithelial and sub-epithelial hemorrhage were lower than in CYP/SAL treated rats. The scores for macroscopic and microscopic evaluation of bladder and ureter were significantly lower in CYP/SIMV rats than in CYP/SAL rats. The kidney was not affected. The expression of TNF-a, IL-1b and IL-6 was significatly lower in CF/SINV rats (164.8±22, 44.8±8 and 52.4±13) than in CF/SAL rats (378.5±66, 122.9±26 e 123.6±18), respectively. Conclusion: The results of the current study suggest that simvastatin pretreatment attenuated CYP-induced urotelium inflammation and decreased the activities of cytokines
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2013-09-26T14:07:53Z
dc.date.available.fl_str_mv 2013-09-26T14:07:53Z
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dc.identifier.citation.fl_str_mv DANTAS, Anna Carolina Batista ; BATISTA JÚNIOR, Francisco Fábio de Araújo ; MACEDO, Larissa Freitas ; MENDES, Mariana Noronha Castro ; AZEVEDO, Ítalo Medeiros ; MEDEIROS, Aldo Cunha . Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats. Acta Cirurgica Brasileira (Impresso) , v. 25, p. 43-46, 2010. Disponível em:<http://www.scielo.br/scielo.php?pid=S0102-86502010000100011&script=sci_arttext> Acesso em: 25 set. 2013.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/jspui/handle/1/6283
dc.identifier.issn.none.fl_str_mv 1678-2674
identifier_str_mv DANTAS, Anna Carolina Batista ; BATISTA JÚNIOR, Francisco Fábio de Araújo ; MACEDO, Larissa Freitas ; MENDES, Mariana Noronha Castro ; AZEVEDO, Ítalo Medeiros ; MEDEIROS, Aldo Cunha . Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats. Acta Cirurgica Brasileira (Impresso) , v. 25, p. 43-46, 2010. Disponível em:<http://www.scielo.br/scielo.php?pid=S0102-86502010000100011&script=sci_arttext> Acesso em: 25 set. 2013.
1678-2674
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