Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/1/6283 |
Resumo: | Cyclophosphamide (CYP) is an antineoplastic agent used for the treatment of many neoplastic and inflammatory diseases. Hemorrhagic cystitis is a frequent side effect of CYP. Several studies show that simvastatin has important pleiotropic (anti-inflammatory and immunomodulatory) effects. The purpose of the study was to investigate the effect of simvastatin on bladder, ureter and kidney injury caused by CYP. Methods: Adult male Wistar rats were randomly divided into three groups. The CYP/SIM group received simvastatin microemulsion by gavage during 7 days (10 mg/kg body wt) before the administration of CYP and the CYP/SAL group rats received saline 0.9%. The control rats were not treated. After that, all rats were treated with a single dose of CYP 200 mg/kg body wt intraperitoneally. The rats were killed 24 h after CYP administration. Plasma cytokines (TNF-a, IL-1b, IL-6) were measured by ELISA. Macro and light microscopic study was performed in the bladder, kidney and ureter. Results: In the bladders of CYP/SIMV treated rats edema of lamina propria with epithelial and sub-epithelial hemorrhage were lower than in CYP/SAL treated rats. The scores for macroscopic and microscopic evaluation of bladder and ureter were significantly lower in CYP/SIMV rats than in CYP/SAL rats. The kidney was not affected. The expression of TNF-a, IL-1b and IL-6 was significatly lower in CF/SINV rats (164.8±22, 44.8±8 and 52.4±13) than in CF/SAL rats (378.5±66, 122.9±26 e 123.6±18), respectively. Conclusion: The results of the current study suggest that simvastatin pretreatment attenuated CYP-induced urotelium inflammation and decreased the activities of cytokines |
id |
UFRN_053f5a16d06902052c8e39dffda649b4 |
---|---|
oai_identifier_str |
oai:https://repositorio.ufrn.br:1/6283 |
network_acronym_str |
UFRN |
network_name_str |
Repositório Institucional da UFRN |
repository_id_str |
|
spelling |
Dantas, Anna Carolina BatistaBatista Júnior, Francisco Fábio de AraújoMacedo, Larissa FreitasMendes, Mariana Noronha CastroAzevedo, Ítalo MedeirosMedeiros, Aldo Cunha2013-09-26T14:07:53Z2013-09-26T14:07:53Z2010DANTAS, Anna Carolina Batista ; BATISTA JÚNIOR, Francisco Fábio de Araújo ; MACEDO, Larissa Freitas ; MENDES, Mariana Noronha Castro ; AZEVEDO, Ítalo Medeiros ; MEDEIROS, Aldo Cunha . Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats. Acta Cirurgica Brasileira (Impresso) , v. 25, p. 43-46, 2010. Disponível em:<http://www.scielo.br/scielo.php?pid=S0102-86502010000100011&script=sci_arttext> Acesso em: 25 set. 2013.1678-2674https://repositorio.ufrn.br/jspui/handle/1/6283Cyclophosphamide (CYP) is an antineoplastic agent used for the treatment of many neoplastic and inflammatory diseases. Hemorrhagic cystitis is a frequent side effect of CYP. Several studies show that simvastatin has important pleiotropic (anti-inflammatory and immunomodulatory) effects. The purpose of the study was to investigate the effect of simvastatin on bladder, ureter and kidney injury caused by CYP. Methods: Adult male Wistar rats were randomly divided into three groups. The CYP/SIM group received simvastatin microemulsion by gavage during 7 days (10 mg/kg body wt) before the administration of CYP and the CYP/SAL group rats received saline 0.9%. The control rats were not treated. After that, all rats were treated with a single dose of CYP 200 mg/kg body wt intraperitoneally. The rats were killed 24 h after CYP administration. Plasma cytokines (TNF-a, IL-1b, IL-6) were measured by ELISA. Macro and light microscopic study was performed in the bladder, kidney and ureter. Results: In the bladders of CYP/SIMV treated rats edema of lamina propria with epithelial and sub-epithelial hemorrhage were lower than in CYP/SAL treated rats. The scores for macroscopic and microscopic evaluation of bladder and ureter were significantly lower in CYP/SIMV rats than in CYP/SAL rats. The kidney was not affected. The expression of TNF-a, IL-1b and IL-6 was significatly lower in CF/SINV rats (164.8±22, 44.8±8 and 52.4±13) than in CF/SAL rats (378.5±66, 122.9±26 e 123.6±18), respectively. Conclusion: The results of the current study suggest that simvastatin pretreatment attenuated CYP-induced urotelium inflammation and decreased the activities of cytokinesengCyclophosphamideCystitisSimvastatinInflammationCytokineRatsProtective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in ratsEfeito protetor da sinvastatina na cistite hemorrágica induzida pela ciclofosfamida em ratosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-823898https://repositorio.ufrn.br/bitstream/1/6283/2/license_rdfe363e809996cf46ada20da1accfcd9c7MD52license_textlicense_texttext/html; charset=utf-80https://repositorio.ufrn.br/bitstream/1/6283/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_urllicense_urltext/plain; charset=utf-852https://repositorio.ufrn.br/bitstream/1/6283/4/license_url3d480ae6c91e310daba2020f8787d6f9MD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufrn.br/bitstream/1/6283/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD55ORIGINALProtectiveEffectSimvastatin_Dantas_2010.pdfProtectiveEffectSimvastatin_Dantas_2010.pdfapplication/pdf2277529https://repositorio.ufrn.br/bitstream/1/6283/1/ProtectiveEffectSimvastatin_Dantas_2010.pdf8d2334a546510c5a76529dc8428c8686MD51TEXTAldoCM_Protecttive_11.pdf.txtAldoCM_Protecttive_11.pdf.txtExtracted texttext/plain22878https://repositorio.ufrn.br/bitstream/1/6283/10/AldoCM_Protecttive_11.pdf.txtb4c33755b6520d59c81353bff511328bMD510THUMBNAILAldoCM_Protecttive_11.pdf.jpgAldoCM_Protecttive_11.pdf.jpgIM Thumbnailimage/jpeg11383https://repositorio.ufrn.br/bitstream/1/6283/11/AldoCM_Protecttive_11.pdf.jpgffe1553922d907799e0581a89f8d91d9MD5111/62832021-11-30 17:55:55.173oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-11-30T20:55:55Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats |
dc.title.alternative.pt_BR.fl_str_mv |
Efeito protetor da sinvastatina na cistite hemorrágica induzida pela ciclofosfamida em ratos |
title |
Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats |
spellingShingle |
Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats Dantas, Anna Carolina Batista Cyclophosphamide Cystitis Simvastatin Inflammation Cytokine Rats |
title_short |
Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats |
title_full |
Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats |
title_fullStr |
Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats |
title_full_unstemmed |
Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats |
title_sort |
Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats |
author |
Dantas, Anna Carolina Batista |
author_facet |
Dantas, Anna Carolina Batista Batista Júnior, Francisco Fábio de Araújo Macedo, Larissa Freitas Mendes, Mariana Noronha Castro Azevedo, Ítalo Medeiros Medeiros, Aldo Cunha |
author_role |
author |
author2 |
Batista Júnior, Francisco Fábio de Araújo Macedo, Larissa Freitas Mendes, Mariana Noronha Castro Azevedo, Ítalo Medeiros Medeiros, Aldo Cunha |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Dantas, Anna Carolina Batista Batista Júnior, Francisco Fábio de Araújo Macedo, Larissa Freitas Mendes, Mariana Noronha Castro Azevedo, Ítalo Medeiros Medeiros, Aldo Cunha |
dc.subject.por.fl_str_mv |
Cyclophosphamide Cystitis Simvastatin Inflammation Cytokine Rats |
topic |
Cyclophosphamide Cystitis Simvastatin Inflammation Cytokine Rats |
description |
Cyclophosphamide (CYP) is an antineoplastic agent used for the treatment of many neoplastic and inflammatory diseases. Hemorrhagic cystitis is a frequent side effect of CYP. Several studies show that simvastatin has important pleiotropic (anti-inflammatory and immunomodulatory) effects. The purpose of the study was to investigate the effect of simvastatin on bladder, ureter and kidney injury caused by CYP. Methods: Adult male Wistar rats were randomly divided into three groups. The CYP/SIM group received simvastatin microemulsion by gavage during 7 days (10 mg/kg body wt) before the administration of CYP and the CYP/SAL group rats received saline 0.9%. The control rats were not treated. After that, all rats were treated with a single dose of CYP 200 mg/kg body wt intraperitoneally. The rats were killed 24 h after CYP administration. Plasma cytokines (TNF-a, IL-1b, IL-6) were measured by ELISA. Macro and light microscopic study was performed in the bladder, kidney and ureter. Results: In the bladders of CYP/SIMV treated rats edema of lamina propria with epithelial and sub-epithelial hemorrhage were lower than in CYP/SAL treated rats. The scores for macroscopic and microscopic evaluation of bladder and ureter were significantly lower in CYP/SIMV rats than in CYP/SAL rats. The kidney was not affected. The expression of TNF-a, IL-1b and IL-6 was significatly lower in CF/SINV rats (164.8±22, 44.8±8 and 52.4±13) than in CF/SAL rats (378.5±66, 122.9±26 e 123.6±18), respectively. Conclusion: The results of the current study suggest that simvastatin pretreatment attenuated CYP-induced urotelium inflammation and decreased the activities of cytokines |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010 |
dc.date.accessioned.fl_str_mv |
2013-09-26T14:07:53Z |
dc.date.available.fl_str_mv |
2013-09-26T14:07:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
DANTAS, Anna Carolina Batista ; BATISTA JÚNIOR, Francisco Fábio de Araújo ; MACEDO, Larissa Freitas ; MENDES, Mariana Noronha Castro ; AZEVEDO, Ítalo Medeiros ; MEDEIROS, Aldo Cunha . Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats. Acta Cirurgica Brasileira (Impresso) , v. 25, p. 43-46, 2010. Disponível em:<http://www.scielo.br/scielo.php?pid=S0102-86502010000100011&script=sci_arttext> Acesso em: 25 set. 2013. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/1/6283 |
dc.identifier.issn.none.fl_str_mv |
1678-2674 |
identifier_str_mv |
DANTAS, Anna Carolina Batista ; BATISTA JÚNIOR, Francisco Fábio de Araújo ; MACEDO, Larissa Freitas ; MENDES, Mariana Noronha Castro ; AZEVEDO, Ítalo Medeiros ; MEDEIROS, Aldo Cunha . Protective effect of simvastatin in the cyclophosphamide-induced hemohrragic cystitis in rats. Acta Cirurgica Brasileira (Impresso) , v. 25, p. 43-46, 2010. Disponível em:<http://www.scielo.br/scielo.php?pid=S0102-86502010000100011&script=sci_arttext> Acesso em: 25 set. 2013. 1678-2674 |
url |
https://repositorio.ufrn.br/jspui/handle/1/6283 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRN instname:Universidade Federal do Rio Grande do Norte (UFRN) instacron:UFRN |
instname_str |
Universidade Federal do Rio Grande do Norte (UFRN) |
instacron_str |
UFRN |
institution |
UFRN |
reponame_str |
Repositório Institucional da UFRN |
collection |
Repositório Institucional da UFRN |
bitstream.url.fl_str_mv |
https://repositorio.ufrn.br/bitstream/1/6283/2/license_rdf https://repositorio.ufrn.br/bitstream/1/6283/3/license_text https://repositorio.ufrn.br/bitstream/1/6283/4/license_url https://repositorio.ufrn.br/bitstream/1/6283/5/license.txt https://repositorio.ufrn.br/bitstream/1/6283/1/ProtectiveEffectSimvastatin_Dantas_2010.pdf https://repositorio.ufrn.br/bitstream/1/6283/10/AldoCM_Protecttive_11.pdf.txt https://repositorio.ufrn.br/bitstream/1/6283/11/AldoCM_Protecttive_11.pdf.jpg |
bitstream.checksum.fl_str_mv |
e363e809996cf46ada20da1accfcd9c7 d41d8cd98f00b204e9800998ecf8427e 3d480ae6c91e310daba2020f8787d6f9 8a4605be74aa9ea9d79846c1fba20a33 8d2334a546510c5a76529dc8428c8686 b4c33755b6520d59c81353bff511328b ffe1553922d907799e0581a89f8d91d9 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN) |
repository.mail.fl_str_mv |
|
_version_ |
1814832848710926336 |