A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells

Detalhes bibliográficos
Autor(a) principal: Morais, Ana Heloneida de Araújo
Data de Publicação: 2012
Outros Autores: Rabelo, Luciana, Monteiro, Norberto de Kássio Vieira, Serquiz, Raphael Paschoal, Santos, Paula, Oliveira, Ruth, Oliveira, Adeliana Silva de, Rocha, Hugo, Uchôa, Adriana Ferreira, Santos, Elizeu Antunes dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/58138
http://dx.doi.org/10.3390/md10040727
Resumo: Cancer represents a set of more than 100 diseases, including malignant tumors from different locations. Strategies inducing differentiation have had limited success in the treatment of established cancers. Marine sponges are a biological reservoir of bioactive molecules, especially lectins. Several animal and plant lectins were purified with antitumor activity, mitogenic, anti-inflammatory and antiviral, but there are few reports in the literature describing the mechanism of action of lectins purified from marine sponges to induce apoptosis in human tumor cells. In this work, a lectin purified from the marine sponge Cinachyrella apion (CaL) was evaluated with respect to its hemolytic, cytotoxic and antiproliferative properties, besides the ability to induce cell death in tumor cells. The antiproliferative activity of CaL was tested against HeLa, PC3 and 3T3 cell lines, with highest growth inhibition for HeLa, reducing cell growth at a dose dependent manner (0.5–10 µg/mL). Hemolytic activity and toxicity against peripheral blood cells were tested using the concentration of IC50 (10 µg/mL) for both trials and twice the IC50 for analysis in flow cytometry, indicating that CaL is not toxic to these cells. To assess the mechanism of cell death caused by CaL in HeLa cells, we performed flow cytometry and western blotting. Results showed that lectin probably induces cell death by apoptosis activation by pro-apoptotic protein Bax, promoting mitochondrial membrane permeabilization, cell cycle arrest in S phase and acting as both dependent and/or independent of caspases pathway. These results indicate the potential of CaL in studies of medicine for treating cancer
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spelling Morais, Ana Heloneida de AraújoRabelo, LucianaMonteiro, Norberto de Kássio VieiraSerquiz, Raphael PaschoalSantos, PaulaOliveira, RuthOliveira, Adeliana Silva deRocha, HugoUchôa, Adriana FerreiraSantos, Elizeu Antunes dos2024-04-12T21:25:01Z2024-04-12T21:25:01Z2012-03RABELO, Luciana; MONTEIRO, Norberto de Kássio Vieira; SERQUIZ, Raphael Paschoal; SANTOS, Paula; OLIVEIRA, Ruth; OLIVEIRA, Adeliana Silva de; ROCHA, Hugo; MORAIS, Ana Heloneida de Araújo; UCHÔA, Adriana Ferreira; SANTOS, Elizeu Antunes dos. A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells. Marine Drugs, [S.l.], v. 10, n. 12, p. 727-743, 28 mar. 2012. DOI: 10.3390/md10040727. Disponível em: https://www.mdpi.com/1660-3397/10/4/727. Acesso em: 11 abr. 2024.https://repositorio.ufrn.br/handle/123456789/58138http://dx.doi.org/10.3390/md10040727Marine DrugsAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessHeLaLectinAntitumorMarine spongeCinachyrella apionA lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCancer represents a set of more than 100 diseases, including malignant tumors from different locations. Strategies inducing differentiation have had limited success in the treatment of established cancers. Marine sponges are a biological reservoir of bioactive molecules, especially lectins. Several animal and plant lectins were purified with antitumor activity, mitogenic, anti-inflammatory and antiviral, but there are few reports in the literature describing the mechanism of action of lectins purified from marine sponges to induce apoptosis in human tumor cells. In this work, a lectin purified from the marine sponge Cinachyrella apion (CaL) was evaluated with respect to its hemolytic, cytotoxic and antiproliferative properties, besides the ability to induce cell death in tumor cells. The antiproliferative activity of CaL was tested against HeLa, PC3 and 3T3 cell lines, with highest growth inhibition for HeLa, reducing cell growth at a dose dependent manner (0.5–10 µg/mL). Hemolytic activity and toxicity against peripheral blood cells were tested using the concentration of IC50 (10 µg/mL) for both trials and twice the IC50 for analysis in flow cytometry, indicating that CaL is not toxic to these cells. To assess the mechanism of cell death caused by CaL in HeLa cells, we performed flow cytometry and western blotting. Results showed that lectin probably induces cell death by apoptosis activation by pro-apoptotic protein Bax, promoting mitochondrial membrane permeabilization, cell cycle arrest in S phase and acting as both dependent and/or independent of caspases pathway. These results indicate the potential of CaL in studies of medicine for treating cancerengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALLactoseBinding_Rabelo_2012.pdfLactoseBinding_Rabelo_2012.pdfapplication/pdf3031055https://repositorio.ufrn.br/bitstream/123456789/58138/1/LactoseBinding_Rabelo_2012.pdf5e66934fed4e90b9db65ff5e6b6c6a7cMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/58138/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/58138/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53123456789/581382024-04-12 18:25:02.551oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2024-04-12T21:25:02Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells
title A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells
spellingShingle A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells
Morais, Ana Heloneida de Araújo
HeLa
Lectin
Antitumor
Marine sponge
Cinachyrella apion
title_short A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells
title_full A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells
title_fullStr A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells
title_full_unstemmed A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells
title_sort A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells
author Morais, Ana Heloneida de Araújo
author_facet Morais, Ana Heloneida de Araújo
Rabelo, Luciana
Monteiro, Norberto de Kássio Vieira
Serquiz, Raphael Paschoal
Santos, Paula
Oliveira, Ruth
Oliveira, Adeliana Silva de
Rocha, Hugo
Uchôa, Adriana Ferreira
Santos, Elizeu Antunes dos
author_role author
author2 Rabelo, Luciana
Monteiro, Norberto de Kássio Vieira
Serquiz, Raphael Paschoal
Santos, Paula
Oliveira, Ruth
Oliveira, Adeliana Silva de
Rocha, Hugo
Uchôa, Adriana Ferreira
Santos, Elizeu Antunes dos
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Morais, Ana Heloneida de Araújo
Rabelo, Luciana
Monteiro, Norberto de Kássio Vieira
Serquiz, Raphael Paschoal
Santos, Paula
Oliveira, Ruth
Oliveira, Adeliana Silva de
Rocha, Hugo
Uchôa, Adriana Ferreira
Santos, Elizeu Antunes dos
dc.subject.por.fl_str_mv HeLa
Lectin
Antitumor
Marine sponge
Cinachyrella apion
topic HeLa
Lectin
Antitumor
Marine sponge
Cinachyrella apion
description Cancer represents a set of more than 100 diseases, including malignant tumors from different locations. Strategies inducing differentiation have had limited success in the treatment of established cancers. Marine sponges are a biological reservoir of bioactive molecules, especially lectins. Several animal and plant lectins were purified with antitumor activity, mitogenic, anti-inflammatory and antiviral, but there are few reports in the literature describing the mechanism of action of lectins purified from marine sponges to induce apoptosis in human tumor cells. In this work, a lectin purified from the marine sponge Cinachyrella apion (CaL) was evaluated with respect to its hemolytic, cytotoxic and antiproliferative properties, besides the ability to induce cell death in tumor cells. The antiproliferative activity of CaL was tested against HeLa, PC3 and 3T3 cell lines, with highest growth inhibition for HeLa, reducing cell growth at a dose dependent manner (0.5–10 µg/mL). Hemolytic activity and toxicity against peripheral blood cells were tested using the concentration of IC50 (10 µg/mL) for both trials and twice the IC50 for analysis in flow cytometry, indicating that CaL is not toxic to these cells. To assess the mechanism of cell death caused by CaL in HeLa cells, we performed flow cytometry and western blotting. Results showed that lectin probably induces cell death by apoptosis activation by pro-apoptotic protein Bax, promoting mitochondrial membrane permeabilization, cell cycle arrest in S phase and acting as both dependent and/or independent of caspases pathway. These results indicate the potential of CaL in studies of medicine for treating cancer
publishDate 2012
dc.date.issued.fl_str_mv 2012-03
dc.date.accessioned.fl_str_mv 2024-04-12T21:25:01Z
dc.date.available.fl_str_mv 2024-04-12T21:25:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv RABELO, Luciana; MONTEIRO, Norberto de Kássio Vieira; SERQUIZ, Raphael Paschoal; SANTOS, Paula; OLIVEIRA, Ruth; OLIVEIRA, Adeliana Silva de; ROCHA, Hugo; MORAIS, Ana Heloneida de Araújo; UCHÔA, Adriana Ferreira; SANTOS, Elizeu Antunes dos. A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells. Marine Drugs, [S.l.], v. 10, n. 12, p. 727-743, 28 mar. 2012. DOI: 10.3390/md10040727. Disponível em: https://www.mdpi.com/1660-3397/10/4/727. Acesso em: 11 abr. 2024.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/58138
dc.identifier.doi.none.fl_str_mv http://dx.doi.org/10.3390/md10040727
identifier_str_mv RABELO, Luciana; MONTEIRO, Norberto de Kássio Vieira; SERQUIZ, Raphael Paschoal; SANTOS, Paula; OLIVEIRA, Ruth; OLIVEIRA, Adeliana Silva de; ROCHA, Hugo; MORAIS, Ana Heloneida de Araújo; UCHÔA, Adriana Ferreira; SANTOS, Elizeu Antunes dos. A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells. Marine Drugs, [S.l.], v. 10, n. 12, p. 727-743, 28 mar. 2012. DOI: 10.3390/md10040727. Disponível em: https://www.mdpi.com/1660-3397/10/4/727. Acesso em: 11 abr. 2024.
url https://repositorio.ufrn.br/handle/123456789/58138
http://dx.doi.org/10.3390/md10040727
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http://creativecommons.org/licenses/by/3.0/br/
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