A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/handle/123456789/58138 http://dx.doi.org/10.3390/md10040727 |
Resumo: | Cancer represents a set of more than 100 diseases, including malignant tumors from different locations. Strategies inducing differentiation have had limited success in the treatment of established cancers. Marine sponges are a biological reservoir of bioactive molecules, especially lectins. Several animal and plant lectins were purified with antitumor activity, mitogenic, anti-inflammatory and antiviral, but there are few reports in the literature describing the mechanism of action of lectins purified from marine sponges to induce apoptosis in human tumor cells. In this work, a lectin purified from the marine sponge Cinachyrella apion (CaL) was evaluated with respect to its hemolytic, cytotoxic and antiproliferative properties, besides the ability to induce cell death in tumor cells. The antiproliferative activity of CaL was tested against HeLa, PC3 and 3T3 cell lines, with highest growth inhibition for HeLa, reducing cell growth at a dose dependent manner (0.5–10 µg/mL). Hemolytic activity and toxicity against peripheral blood cells were tested using the concentration of IC50 (10 µg/mL) for both trials and twice the IC50 for analysis in flow cytometry, indicating that CaL is not toxic to these cells. To assess the mechanism of cell death caused by CaL in HeLa cells, we performed flow cytometry and western blotting. Results showed that lectin probably induces cell death by apoptosis activation by pro-apoptotic protein Bax, promoting mitochondrial membrane permeabilization, cell cycle arrest in S phase and acting as both dependent and/or independent of caspases pathway. These results indicate the potential of CaL in studies of medicine for treating cancer |
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Morais, Ana Heloneida de AraújoRabelo, LucianaMonteiro, Norberto de Kássio VieiraSerquiz, Raphael PaschoalSantos, PaulaOliveira, RuthOliveira, Adeliana Silva deRocha, HugoUchôa, Adriana FerreiraSantos, Elizeu Antunes dos2024-04-12T21:25:01Z2024-04-12T21:25:01Z2012-03RABELO, Luciana; MONTEIRO, Norberto de Kássio Vieira; SERQUIZ, Raphael Paschoal; SANTOS, Paula; OLIVEIRA, Ruth; OLIVEIRA, Adeliana Silva de; ROCHA, Hugo; MORAIS, Ana Heloneida de Araújo; UCHÔA, Adriana Ferreira; SANTOS, Elizeu Antunes dos. A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells. Marine Drugs, [S.l.], v. 10, n. 12, p. 727-743, 28 mar. 2012. DOI: 10.3390/md10040727. Disponível em: https://www.mdpi.com/1660-3397/10/4/727. Acesso em: 11 abr. 2024.https://repositorio.ufrn.br/handle/123456789/58138http://dx.doi.org/10.3390/md10040727Marine DrugsAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessHeLaLectinAntitumorMarine spongeCinachyrella apionA lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCancer represents a set of more than 100 diseases, including malignant tumors from different locations. Strategies inducing differentiation have had limited success in the treatment of established cancers. Marine sponges are a biological reservoir of bioactive molecules, especially lectins. Several animal and plant lectins were purified with antitumor activity, mitogenic, anti-inflammatory and antiviral, but there are few reports in the literature describing the mechanism of action of lectins purified from marine sponges to induce apoptosis in human tumor cells. In this work, a lectin purified from the marine sponge Cinachyrella apion (CaL) was evaluated with respect to its hemolytic, cytotoxic and antiproliferative properties, besides the ability to induce cell death in tumor cells. The antiproliferative activity of CaL was tested against HeLa, PC3 and 3T3 cell lines, with highest growth inhibition for HeLa, reducing cell growth at a dose dependent manner (0.5–10 µg/mL). Hemolytic activity and toxicity against peripheral blood cells were tested using the concentration of IC50 (10 µg/mL) for both trials and twice the IC50 for analysis in flow cytometry, indicating that CaL is not toxic to these cells. To assess the mechanism of cell death caused by CaL in HeLa cells, we performed flow cytometry and western blotting. Results showed that lectin probably induces cell death by apoptosis activation by pro-apoptotic protein Bax, promoting mitochondrial membrane permeabilization, cell cycle arrest in S phase and acting as both dependent and/or independent of caspases pathway. These results indicate the potential of CaL in studies of medicine for treating cancerengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALLactoseBinding_Rabelo_2012.pdfLactoseBinding_Rabelo_2012.pdfapplication/pdf3031055https://repositorio.ufrn.br/bitstream/123456789/58138/1/LactoseBinding_Rabelo_2012.pdf5e66934fed4e90b9db65ff5e6b6c6a7cMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/58138/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/58138/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53123456789/581382024-04-12 18:25:02.551oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2024-04-12T21:25:02Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells |
title |
A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells |
spellingShingle |
A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells Morais, Ana Heloneida de Araújo HeLa Lectin Antitumor Marine sponge Cinachyrella apion |
title_short |
A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells |
title_full |
A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells |
title_fullStr |
A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells |
title_full_unstemmed |
A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells |
title_sort |
A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells |
author |
Morais, Ana Heloneida de Araújo |
author_facet |
Morais, Ana Heloneida de Araújo Rabelo, Luciana Monteiro, Norberto de Kássio Vieira Serquiz, Raphael Paschoal Santos, Paula Oliveira, Ruth Oliveira, Adeliana Silva de Rocha, Hugo Uchôa, Adriana Ferreira Santos, Elizeu Antunes dos |
author_role |
author |
author2 |
Rabelo, Luciana Monteiro, Norberto de Kássio Vieira Serquiz, Raphael Paschoal Santos, Paula Oliveira, Ruth Oliveira, Adeliana Silva de Rocha, Hugo Uchôa, Adriana Ferreira Santos, Elizeu Antunes dos |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Morais, Ana Heloneida de Araújo Rabelo, Luciana Monteiro, Norberto de Kássio Vieira Serquiz, Raphael Paschoal Santos, Paula Oliveira, Ruth Oliveira, Adeliana Silva de Rocha, Hugo Uchôa, Adriana Ferreira Santos, Elizeu Antunes dos |
dc.subject.por.fl_str_mv |
HeLa Lectin Antitumor Marine sponge Cinachyrella apion |
topic |
HeLa Lectin Antitumor Marine sponge Cinachyrella apion |
description |
Cancer represents a set of more than 100 diseases, including malignant tumors from different locations. Strategies inducing differentiation have had limited success in the treatment of established cancers. Marine sponges are a biological reservoir of bioactive molecules, especially lectins. Several animal and plant lectins were purified with antitumor activity, mitogenic, anti-inflammatory and antiviral, but there are few reports in the literature describing the mechanism of action of lectins purified from marine sponges to induce apoptosis in human tumor cells. In this work, a lectin purified from the marine sponge Cinachyrella apion (CaL) was evaluated with respect to its hemolytic, cytotoxic and antiproliferative properties, besides the ability to induce cell death in tumor cells. The antiproliferative activity of CaL was tested against HeLa, PC3 and 3T3 cell lines, with highest growth inhibition for HeLa, reducing cell growth at a dose dependent manner (0.5–10 µg/mL). Hemolytic activity and toxicity against peripheral blood cells were tested using the concentration of IC50 (10 µg/mL) for both trials and twice the IC50 for analysis in flow cytometry, indicating that CaL is not toxic to these cells. To assess the mechanism of cell death caused by CaL in HeLa cells, we performed flow cytometry and western blotting. Results showed that lectin probably induces cell death by apoptosis activation by pro-apoptotic protein Bax, promoting mitochondrial membrane permeabilization, cell cycle arrest in S phase and acting as both dependent and/or independent of caspases pathway. These results indicate the potential of CaL in studies of medicine for treating cancer |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-03 |
dc.date.accessioned.fl_str_mv |
2024-04-12T21:25:01Z |
dc.date.available.fl_str_mv |
2024-04-12T21:25:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RABELO, Luciana; MONTEIRO, Norberto de Kássio Vieira; SERQUIZ, Raphael Paschoal; SANTOS, Paula; OLIVEIRA, Ruth; OLIVEIRA, Adeliana Silva de; ROCHA, Hugo; MORAIS, Ana Heloneida de Araújo; UCHÔA, Adriana Ferreira; SANTOS, Elizeu Antunes dos. A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells. Marine Drugs, [S.l.], v. 10, n. 12, p. 727-743, 28 mar. 2012. DOI: 10.3390/md10040727. Disponível em: https://www.mdpi.com/1660-3397/10/4/727. Acesso em: 11 abr. 2024. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/handle/123456789/58138 |
dc.identifier.doi.none.fl_str_mv |
http://dx.doi.org/10.3390/md10040727 |
identifier_str_mv |
RABELO, Luciana; MONTEIRO, Norberto de Kássio Vieira; SERQUIZ, Raphael Paschoal; SANTOS, Paula; OLIVEIRA, Ruth; OLIVEIRA, Adeliana Silva de; ROCHA, Hugo; MORAIS, Ana Heloneida de Araújo; UCHÔA, Adriana Ferreira; SANTOS, Elizeu Antunes dos. A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells. Marine Drugs, [S.l.], v. 10, n. 12, p. 727-743, 28 mar. 2012. DOI: 10.3390/md10040727. Disponível em: https://www.mdpi.com/1660-3397/10/4/727. Acesso em: 11 abr. 2024. |
url |
https://repositorio.ufrn.br/handle/123456789/58138 http://dx.doi.org/10.3390/md10040727 |
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eng |
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eng |
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Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution 3.0 Brazil http://creativecommons.org/licenses/by/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Marine Drugs |
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Marine Drugs |
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