Tamarind multifunctional protein: safety and anti-inflammatory potential in intestinal mucosa and adipose tissue in a preclinical model of diet-induced obesity

• Autor(a) principal: Morais, Ana Heloneida de Araújo
• Data de Publicação: 2021
• Outros Autores: Lima, Vanessa C. O., Amarante, Maria do Socorro M., Lima, Maíra C. J. S., Carvalho, Fabiana M. C., Figueredo, Julia B. S., Santos, Pedro P. A., Camillo, Christina S., Ladd, Fernando V. L., Maciel, Bruna L. L., Uchôa, Adriana F.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRN
• Texto Completo: https://repositorio.ufrn.br/handle/123456789/54403
• topic: Obesity; trypsin inhibitor; intestine; toxicity; visceral fat · Imflammation

Introduction: Obesity has emerged as one of the main pub lic health problems. This condition triggers a series of hor monal and metabolic changes related to a low-grade chron ic inflammatory condition. The trypsin inhibitor purified from tamarind (TTIp) seeds is a promising anti-inflammatory molecule, but its safety needs to be evaluated. This study aimed to evaluate TTIp bioactive dose effects on organs in volved in its metabolism (liver and pancreas) and affected tissues (small intestine and perirenal adipose tissue) in an obesity model. Methods: Three groups of adult male Wistar rats were used (n = 5). Two of these groups had diet-induced obesity, and a third group was eutrophic. TTIp was adminis tered by gavage in one of the obese groups for 10 days, while the remaining groups received a vehicle. The chromato graphic profile and the inhibition assay corroded the purifi cation of the inhibitor. Physical and behavioral changes, liver enzymes, and stereological and histopathological analyses of tissues were evaluated. Results: TTIp did not cause visible signs of toxicity, nor caused changes in liver enzymes, the liver, and pancreatic tissues. TTIp did not cause changes in the intestinal mucosa, showing improvement in the villi’s histopathological characteristics compared to the group of animals with obesity without treatment with TTIp (p = 0.004). The analysis of perirenal adipose tissue showed that the av erage sectional area of animals with obesity that received TTIp did not differ from the control. There was a difference between the high glycemic load diet group and the group treated with the inhibitor (351.8 ± 55.5) (p = 0.016). In addi tion, the group that received TTIp had no inflammatory infil trates. Conclusion: Based on histological and stereological analysis, the use of TTIp is potentially safe and anti-inflam matory in the evaluated obesity model and can be investi gated as a possible adjuvant in obesity therapy.