Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/handle/123456789/57706 http://dx.doi.org/10.1017/s0007114522001271 |
Resumo: | Few studies have focused on nutrient-deficient diets and associated pathobiological dynamics of body composition and intestinal barrier function. This study evaluated the impact of a nutrient-deficient diet on physical development and intestinal morphofunctional barrier in mice. C57BL/6 (21 days of age) mice were fed a Northeastern Brazil regional basic diet (RBD) or a control diet for 21 d. The animals were subjected to bioimpedance analysis, lactulose test, morphometric analysis and quantitative reverse transcription-PCR to evaluate tight junctions and intestinal transporters. RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14. The intake of RBD reduced total body water, extracellular fluid, fat mass and fat-free mass from day 7 (P < 0·05). RBD induced changes in the jejunum, with an increase in the villus:crypt ratio on day 7, followed by reduction on days 14 and 21 (P < 0·05). Lactulose:mannitol ratio increased on day 14 (P < 0·05). Changes in intestinal barrier function on day 14 were associated with reductions in claudin-1 and occludin, and on day 21, there was a reduction in the levels of claudin-2 and occludin. SGLT-1 levels decreased on day 21. RBD compromises body composition and physical development with dynamic changes in intestinal barrier morphofunctional. RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic period |
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Maciel, Bruna Leal LimaRibeiro, Samilly AlbuquerqueRodrigues, Francisco Adelvane de PaulaClementino, Marco Antonio de FreitasVeras, Herlice do NascimentoSiqueira, Rômmulo Celly LimaMedeiros, Pedro Henrique Quintela Soares dePereira, Jeanine MoraisGuanabara Júnior, Márcio Flávio AraújoSousa, José Kleybson deSantos, Ana Karolina Silva2024-02-28T18:53:26Z2024-02-28T18:53:26Z2022-04RIBEIRO, Samilly Albuquerque; RODRIGUES, Francisco Adelvane de Paula; CLEMENTINO, Marco Antonio de Freitas; VERAS, Herlice do Nascimento; SIQUEIRA, Rômmulo Celly Lima; MEDEIROS, Pedro Henrique Quintela Soares de; PEREIRA, Jeanine Morais; GUANABARA JÚNIOR, Márcio Flávio Araújo; SOUSA, José Kleybson de; SANTOS, Ana Karolina Silva. Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice. British Journal of Nutrition, [S.l.], v. 129, n. 5, p. 745-758, 29 abr. 2022. DOI: 10.1017/s0007114522001271. Disponível em: https://www.cambridge.org/core/journals/british-journal-of-nutrition/article/abs/consumption-of-a-multideficient-diet-causes-dynamic-changes-in-the-intestinal-morphofunctional-barrier-body-composition-and-impaired-physical-development-in-postweaning-mice/8A0140363AAD61B20EF888DCF02E76B1. Acesso em: 6 fev. 2024.https://repositorio.ufrn.br/handle/123456789/57706http://dx.doi.org/10.1017/s0007114522001271British Journal of NutritionMalnutritionNortheastern Brazil regional basic dietIntestinal barrier functionBody compositionTight junction proteinsConsumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFew studies have focused on nutrient-deficient diets and associated pathobiological dynamics of body composition and intestinal barrier function. This study evaluated the impact of a nutrient-deficient diet on physical development and intestinal morphofunctional barrier in mice. C57BL/6 (21 days of age) mice were fed a Northeastern Brazil regional basic diet (RBD) or a control diet for 21 d. The animals were subjected to bioimpedance analysis, lactulose test, morphometric analysis and quantitative reverse transcription-PCR to evaluate tight junctions and intestinal transporters. RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14. The intake of RBD reduced total body water, extracellular fluid, fat mass and fat-free mass from day 7 (P < 0·05). RBD induced changes in the jejunum, with an increase in the villus:crypt ratio on day 7, followed by reduction on days 14 and 21 (P < 0·05). Lactulose:mannitol ratio increased on day 14 (P < 0·05). Changes in intestinal barrier function on day 14 were associated with reductions in claudin-1 and occludin, and on day 21, there was a reduction in the levels of claudin-2 and occludin. SGLT-1 levels decreased on day 21. RBD compromises body composition and physical development with dynamic changes in intestinal barrier morphofunctional. RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic periodengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/57706/2/license.txte9597aa2854d128fd968be5edc8a28d9MD52123456789/577062024-02-28 15:53:46.365oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2024-02-28T18:53:46Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice |
title |
Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice |
spellingShingle |
Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice Maciel, Bruna Leal Lima Malnutrition Northeastern Brazil regional basic diet Intestinal barrier function Body composition Tight junction proteins |
title_short |
Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice |
title_full |
Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice |
title_fullStr |
Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice |
title_full_unstemmed |
Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice |
title_sort |
Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice |
author |
Maciel, Bruna Leal Lima |
author_facet |
Maciel, Bruna Leal Lima Ribeiro, Samilly Albuquerque Rodrigues, Francisco Adelvane de Paula Clementino, Marco Antonio de Freitas Veras, Herlice do Nascimento Siqueira, Rômmulo Celly Lima Medeiros, Pedro Henrique Quintela Soares de Pereira, Jeanine Morais Guanabara Júnior, Márcio Flávio Araújo Sousa, José Kleybson de Santos, Ana Karolina Silva |
author_role |
author |
author2 |
Ribeiro, Samilly Albuquerque Rodrigues, Francisco Adelvane de Paula Clementino, Marco Antonio de Freitas Veras, Herlice do Nascimento Siqueira, Rômmulo Celly Lima Medeiros, Pedro Henrique Quintela Soares de Pereira, Jeanine Morais Guanabara Júnior, Márcio Flávio Araújo Sousa, José Kleybson de Santos, Ana Karolina Silva |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Maciel, Bruna Leal Lima Ribeiro, Samilly Albuquerque Rodrigues, Francisco Adelvane de Paula Clementino, Marco Antonio de Freitas Veras, Herlice do Nascimento Siqueira, Rômmulo Celly Lima Medeiros, Pedro Henrique Quintela Soares de Pereira, Jeanine Morais Guanabara Júnior, Márcio Flávio Araújo Sousa, José Kleybson de Santos, Ana Karolina Silva |
dc.subject.por.fl_str_mv |
Malnutrition Northeastern Brazil regional basic diet Intestinal barrier function Body composition Tight junction proteins |
topic |
Malnutrition Northeastern Brazil regional basic diet Intestinal barrier function Body composition Tight junction proteins |
description |
Few studies have focused on nutrient-deficient diets and associated pathobiological dynamics of body composition and intestinal barrier function. This study evaluated the impact of a nutrient-deficient diet on physical development and intestinal morphofunctional barrier in mice. C57BL/6 (21 days of age) mice were fed a Northeastern Brazil regional basic diet (RBD) or a control diet for 21 d. The animals were subjected to bioimpedance analysis, lactulose test, morphometric analysis and quantitative reverse transcription-PCR to evaluate tight junctions and intestinal transporters. RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14. The intake of RBD reduced total body water, extracellular fluid, fat mass and fat-free mass from day 7 (P < 0·05). RBD induced changes in the jejunum, with an increase in the villus:crypt ratio on day 7, followed by reduction on days 14 and 21 (P < 0·05). Lactulose:mannitol ratio increased on day 14 (P < 0·05). Changes in intestinal barrier function on day 14 were associated with reductions in claudin-1 and occludin, and on day 21, there was a reduction in the levels of claudin-2 and occludin. SGLT-1 levels decreased on day 21. RBD compromises body composition and physical development with dynamic changes in intestinal barrier morphofunctional. RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic period |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022-04 |
dc.date.accessioned.fl_str_mv |
2024-02-28T18:53:26Z |
dc.date.available.fl_str_mv |
2024-02-28T18:53:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RIBEIRO, Samilly Albuquerque; RODRIGUES, Francisco Adelvane de Paula; CLEMENTINO, Marco Antonio de Freitas; VERAS, Herlice do Nascimento; SIQUEIRA, Rômmulo Celly Lima; MEDEIROS, Pedro Henrique Quintela Soares de; PEREIRA, Jeanine Morais; GUANABARA JÚNIOR, Márcio Flávio Araújo; SOUSA, José Kleybson de; SANTOS, Ana Karolina Silva. Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice. British Journal of Nutrition, [S.l.], v. 129, n. 5, p. 745-758, 29 abr. 2022. DOI: 10.1017/s0007114522001271. Disponível em: https://www.cambridge.org/core/journals/british-journal-of-nutrition/article/abs/consumption-of-a-multideficient-diet-causes-dynamic-changes-in-the-intestinal-morphofunctional-barrier-body-composition-and-impaired-physical-development-in-postweaning-mice/8A0140363AAD61B20EF888DCF02E76B1. Acesso em: 6 fev. 2024. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/handle/123456789/57706 |
dc.identifier.doi.none.fl_str_mv |
http://dx.doi.org/10.1017/s0007114522001271 |
identifier_str_mv |
RIBEIRO, Samilly Albuquerque; RODRIGUES, Francisco Adelvane de Paula; CLEMENTINO, Marco Antonio de Freitas; VERAS, Herlice do Nascimento; SIQUEIRA, Rômmulo Celly Lima; MEDEIROS, Pedro Henrique Quintela Soares de; PEREIRA, Jeanine Morais; GUANABARA JÚNIOR, Márcio Flávio Araújo; SOUSA, José Kleybson de; SANTOS, Ana Karolina Silva. Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice. British Journal of Nutrition, [S.l.], v. 129, n. 5, p. 745-758, 29 abr. 2022. DOI: 10.1017/s0007114522001271. Disponível em: https://www.cambridge.org/core/journals/british-journal-of-nutrition/article/abs/consumption-of-a-multideficient-diet-causes-dynamic-changes-in-the-intestinal-morphofunctional-barrier-body-composition-and-impaired-physical-development-in-postweaning-mice/8A0140363AAD61B20EF888DCF02E76B1. Acesso em: 6 fev. 2024. |
url |
https://repositorio.ufrn.br/handle/123456789/57706 http://dx.doi.org/10.1017/s0007114522001271 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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openAccess |
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British Journal of Nutrition |
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British Journal of Nutrition |
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