NMDARs control object recognition memory destabilization and reconsolidation
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/handle/123456789/52229 |
Resumo: | Object recognition memory (ORM) allows identification of previously encountered items and is therefore crucial for remembering episodic information. In rodents, reactivation during recall in the presence of a novel object destabilizes ORM and initiates a Zif268 and protein synthesis-dependent reconsolidation process in the hippocampus that links the memory of this object to the reactivated recognition trace. Hippocampal NMDA receptors (NMDARs) modulate Zif268 expression and protein synthesis and regulate memory stability but their possible involvement in the ORM destabilization/reconsolidation cycle has yet to be analyzed in detail. We found that, in adult male Wistar rats, intra dorsal-CA1 administration of the non-subunit selective NMDAR antagonist AP5, or of the GluN2A subunit-containing NMDAR antagonist TCN201, 5 min after an ORM reactivation session in the presence of a novel object carried out 24 h post-training impaired retention 24 h later. In contrast, pre-reactivation administration of the GluN2B subunit-containing NMDAR antagonist RO25-6981 had no effect on ORM recall or retention but impeded the amnesia caused by Zif268 silencing and protein synthesis inhibition in dorsal CA1. Our results indicate that GluN2B-containing hippocampal NMDARs are necessary for ORM destabilization whereas GluN2A-containing NMDARs are involved in ORM reconsolidation, and suggest that modulation of the relative activity of these receptor subtypes during recall regulates ORM persistence |
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Rossato, Janine InezRadiske, AndressaGonzalez, Maria CarolinaApolinário, Gênedy Karielly da SilvaAraújo, Raquel Lúcia Souto deBevilaqua, Lia Rejane MullerCammarota, Martín Pablo2023-04-25T19:09:10Z2023-04-25T19:09:10Z2023-04ROSSATO, Janine I.; RADISKE, Andressa; GONZALEZ, Maria Carolina; APOLINÁRIO, Gênedy; ARAÚJO, Raquel L. S.; BEVILAQUA, Lia R. M.; CAMMAROTA, Martín. NMDARs control object recognition memory destabilization and reconsolidation. Brain Research Bulletin, [S. l.], v. 197, p. 42-48, jun. 2023. Doi: http://dx.doi.org/10.1016/j.brainresbull.2023.03.013. Disponível em: https://www.sciencedirect.com/science/article/pii/S0361923023000692?via%3Dihub. Acesso em: 25 abr. 2023.https://repositorio.ufrn.br/handle/123456789/5222910.1016/j.brainresbull.2023.03.013Elsevier BVAtribuição-NãoComercial-SemDerivados 3.0 Brasilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessHippocampusRecallTCN201AP5RO25–6981Memory consolidationNMDARs control object recognition memory destabilization and reconsolidationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleObject recognition memory (ORM) allows identification of previously encountered items and is therefore crucial for remembering episodic information. In rodents, reactivation during recall in the presence of a novel object destabilizes ORM and initiates a Zif268 and protein synthesis-dependent reconsolidation process in the hippocampus that links the memory of this object to the reactivated recognition trace. Hippocampal NMDA receptors (NMDARs) modulate Zif268 expression and protein synthesis and regulate memory stability but their possible involvement in the ORM destabilization/reconsolidation cycle has yet to be analyzed in detail. We found that, in adult male Wistar rats, intra dorsal-CA1 administration of the non-subunit selective NMDAR antagonist AP5, or of the GluN2A subunit-containing NMDAR antagonist TCN201, 5 min after an ORM reactivation session in the presence of a novel object carried out 24 h post-training impaired retention 24 h later. In contrast, pre-reactivation administration of the GluN2B subunit-containing NMDAR antagonist RO25-6981 had no effect on ORM recall or retention but impeded the amnesia caused by Zif268 silencing and protein synthesis inhibition in dorsal CA1. Our results indicate that GluN2B-containing hippocampal NMDARs are necessary for ORM destabilization whereas GluN2A-containing NMDARs are involved in ORM reconsolidation, and suggest that modulation of the relative activity of these receptor subtypes during recall regulates ORM persistenceengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALNMDARsControlObject_Cammarota_2023.pdfNMDARsControlObject_Cammarota_2023.pdfNMDARsControlObject_Cammarota_2023application/pdf1997932https://repositorio.ufrn.br/bitstream/123456789/52229/1/NMDARsControlObject_Cammarota_2023.pdf4c4b5679961cb3d0dabbcd54df96c6afMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.ufrn.br/bitstream/123456789/52229/2/license_rdfe39d27027a6cc9cb039ad269a5db8e34MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/52229/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53123456789/522292023-04-25 16:09:11.359oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2023-04-25T19:09:11Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
NMDARs control object recognition memory destabilization and reconsolidation |
title |
NMDARs control object recognition memory destabilization and reconsolidation |
spellingShingle |
NMDARs control object recognition memory destabilization and reconsolidation Rossato, Janine Inez Hippocampus Recall TCN201 AP5 RO25–6981 Memory consolidation |
title_short |
NMDARs control object recognition memory destabilization and reconsolidation |
title_full |
NMDARs control object recognition memory destabilization and reconsolidation |
title_fullStr |
NMDARs control object recognition memory destabilization and reconsolidation |
title_full_unstemmed |
NMDARs control object recognition memory destabilization and reconsolidation |
title_sort |
NMDARs control object recognition memory destabilization and reconsolidation |
author |
Rossato, Janine Inez |
author_facet |
Rossato, Janine Inez Radiske, Andressa Gonzalez, Maria Carolina Apolinário, Gênedy Karielly da Silva Araújo, Raquel Lúcia Souto de Bevilaqua, Lia Rejane Muller Cammarota, Martín Pablo |
author_role |
author |
author2 |
Radiske, Andressa Gonzalez, Maria Carolina Apolinário, Gênedy Karielly da Silva Araújo, Raquel Lúcia Souto de Bevilaqua, Lia Rejane Muller Cammarota, Martín Pablo |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Rossato, Janine Inez Radiske, Andressa Gonzalez, Maria Carolina Apolinário, Gênedy Karielly da Silva Araújo, Raquel Lúcia Souto de Bevilaqua, Lia Rejane Muller Cammarota, Martín Pablo |
dc.subject.por.fl_str_mv |
Hippocampus Recall TCN201 AP5 RO25–6981 Memory consolidation |
topic |
Hippocampus Recall TCN201 AP5 RO25–6981 Memory consolidation |
description |
Object recognition memory (ORM) allows identification of previously encountered items and is therefore crucial for remembering episodic information. In rodents, reactivation during recall in the presence of a novel object destabilizes ORM and initiates a Zif268 and protein synthesis-dependent reconsolidation process in the hippocampus that links the memory of this object to the reactivated recognition trace. Hippocampal NMDA receptors (NMDARs) modulate Zif268 expression and protein synthesis and regulate memory stability but their possible involvement in the ORM destabilization/reconsolidation cycle has yet to be analyzed in detail. We found that, in adult male Wistar rats, intra dorsal-CA1 administration of the non-subunit selective NMDAR antagonist AP5, or of the GluN2A subunit-containing NMDAR antagonist TCN201, 5 min after an ORM reactivation session in the presence of a novel object carried out 24 h post-training impaired retention 24 h later. In contrast, pre-reactivation administration of the GluN2B subunit-containing NMDAR antagonist RO25-6981 had no effect on ORM recall or retention but impeded the amnesia caused by Zif268 silencing and protein synthesis inhibition in dorsal CA1. Our results indicate that GluN2B-containing hippocampal NMDARs are necessary for ORM destabilization whereas GluN2A-containing NMDARs are involved in ORM reconsolidation, and suggest that modulation of the relative activity of these receptor subtypes during recall regulates ORM persistence |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-04-25T19:09:10Z |
dc.date.available.fl_str_mv |
2023-04-25T19:09:10Z |
dc.date.issued.fl_str_mv |
2023-04 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ROSSATO, Janine I.; RADISKE, Andressa; GONZALEZ, Maria Carolina; APOLINÁRIO, Gênedy; ARAÚJO, Raquel L. S.; BEVILAQUA, Lia R. M.; CAMMAROTA, Martín. NMDARs control object recognition memory destabilization and reconsolidation. Brain Research Bulletin, [S. l.], v. 197, p. 42-48, jun. 2023. Doi: http://dx.doi.org/10.1016/j.brainresbull.2023.03.013. Disponível em: https://www.sciencedirect.com/science/article/pii/S0361923023000692?via%3Dihub. Acesso em: 25 abr. 2023. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/handle/123456789/52229 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.brainresbull.2023.03.013 |
identifier_str_mv |
ROSSATO, Janine I.; RADISKE, Andressa; GONZALEZ, Maria Carolina; APOLINÁRIO, Gênedy; ARAÚJO, Raquel L. S.; BEVILAQUA, Lia R. M.; CAMMAROTA, Martín. NMDARs control object recognition memory destabilization and reconsolidation. Brain Research Bulletin, [S. l.], v. 197, p. 42-48, jun. 2023. Doi: http://dx.doi.org/10.1016/j.brainresbull.2023.03.013. Disponível em: https://www.sciencedirect.com/science/article/pii/S0361923023000692?via%3Dihub. Acesso em: 25 abr. 2023. 10.1016/j.brainresbull.2023.03.013 |
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https://repositorio.ufrn.br/handle/123456789/52229 |
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eng |
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eng |
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Atribuição-NãoComercial-SemDerivados 3.0 Brasil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
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openAccess |
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Elsevier BV |
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Elsevier BV |
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