Comparative analysis of the immunohistochemical expression of collagen IV, MMP-9, and TIMP-2 in odontogenic cysts and tumors
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/123456789/21761 |
Resumo: | Objective. The aim of this study was to evaluate the immunohistochemical expression of collagen IV, matrix metalloproteinase (MMP) 9 and tissue inhibitor of MMP (TIMP) 2 in dentigerous cysts (DCs), radicular cysts (RCs), keratocystic odontogenic tumors (KOTs), and ameloblastomas. Study design. Twenty cases of DCs, 20 RCs, 20 KOTs, and 20 ameloblastomas were selected and analyzed by immunohistochemistry. Results. Most DCs and RCs showed continuous and 50% staining for collagen IV in the basement membrane of the epithelium, whereas predominantly discontinuous thin and 50% staining was observed in KOTs and ameloblastomas, with a significant difference in staining percentage (P .001). MMP-9 was diffusely distributed and localized in both epithelial and mesenchymal cells of all of the lesions analyzed. The staining percentage was higher in the epithelium (P .058) and mesenchyme (P .005) of KOTs and ameloblastomas. Moreover, the distribution pattern, location, and percentage of expression of TIMP-2 were similar in the lesions studied, except for ameloblastoma, with a significant difference in staining percentage (P .001). Conclusion. These results demonstrate that the interaction between collagen IV, MMP-9, and TIMP-2 is an important factor for the establishment of differences in the biologic behavior of the odontogenic cysts and tumors studied. |
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Henriques, Águida Cristina GomesVasconcelos, Marcelo GadellaGalvão, Hebel CavalcantiSouza, Lelia Batista deFreitas, Roseana de Almeida2017-01-26T13:47:53Z2017-01-26T13:47:53Z2011HENRIQUES, Águida Cristina Gomes et al. Comparative analysis of the immunohistochemical expression of collagen IV, MMP-9, and TIMP-2 in odontogenic cysts and tumors. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics, v. 112, n. 4, p. 468-475, 2011.https://repositorio.ufrn.br/jspui/handle/123456789/21761engImmunohistochemistryOdontogenic cystsCollagen diseasesMMP-9TIMP-2Comparative analysis of the immunohistochemical expression of collagen IV, MMP-9, and TIMP-2 in odontogenic cysts and tumorsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleObjective. The aim of this study was to evaluate the immunohistochemical expression of collagen IV, matrix metalloproteinase (MMP) 9 and tissue inhibitor of MMP (TIMP) 2 in dentigerous cysts (DCs), radicular cysts (RCs), keratocystic odontogenic tumors (KOTs), and ameloblastomas. Study design. Twenty cases of DCs, 20 RCs, 20 KOTs, and 20 ameloblastomas were selected and analyzed by immunohistochemistry. Results. Most DCs and RCs showed continuous and 50% staining for collagen IV in the basement membrane of the epithelium, whereas predominantly discontinuous thin and 50% staining was observed in KOTs and ameloblastomas, with a significant difference in staining percentage (P .001). MMP-9 was diffusely distributed and localized in both epithelial and mesenchymal cells of all of the lesions analyzed. The staining percentage was higher in the epithelium (P .058) and mesenchyme (P .005) of KOTs and ameloblastomas. Moreover, the distribution pattern, location, and percentage of expression of TIMP-2 were similar in the lesions studied, except for ameloblastoma, with a significant difference in staining percentage (P .001). Conclusion. These results demonstrate that the interaction between collagen IV, MMP-9, and TIMP-2 is an important factor for the establishment of differences in the biologic behavior of the odontogenic cysts and tumors studied.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALComparativeAnalysisImmunohistochemical_Henriques_2011.pdfComparativeAnalysisImmunohistochemical_Henriques_2011.pdfhttp://www.sciencedirect.com/science/article/pii/S1079210411003623application/pdf3175255https://repositorio.ufrn.br/bitstream/123456789/21761/1/ComparativeAnalysisImmunohistochemical_Henriques_2011.pdf18891bc6d0a92061e0e898e0797d269cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81563https://repositorio.ufrn.br/bitstream/123456789/21761/2/license.txt2fca3d993fd069474a9dfb5156c39499MD52TEXTComparative analysis of the immunohistochemical expression_`2011.pdf.txtComparative analysis of the immunohistochemical expression_`2011.pdf.txtExtracted texttext/plain33714https://repositorio.ufrn.br/bitstream/123456789/21761/5/Comparative%20analysis%20of%20the%20immunohistochemical%20expression_%602011.pdf.txtdda048a495ece9d450fe493069ae3aa3MD55THUMBNAILComparative analysis of the immunohistochemical expression_`2011.pdf.jpgComparative analysis of the immunohistochemical expression_`2011.pdf.jpgIM Thumbnailimage/jpeg9068https://repositorio.ufrn.br/bitstream/123456789/21761/6/Comparative%20analysis%20of%20the%20immunohistochemical%20expression_%602011.pdf.jpgc916488d00c32473a2ca8033b7bd9d90MD56123456789/217612021-12-21 14:04:40.72oai:https://repositorio.ufrn.br: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ório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-12-21T17:04:40Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Comparative analysis of the immunohistochemical expression of collagen IV, MMP-9, and TIMP-2 in odontogenic cysts and tumors |
title |
Comparative analysis of the immunohistochemical expression of collagen IV, MMP-9, and TIMP-2 in odontogenic cysts and tumors |
spellingShingle |
Comparative analysis of the immunohistochemical expression of collagen IV, MMP-9, and TIMP-2 in odontogenic cysts and tumors Henriques, Águida Cristina Gomes Immunohistochemistry Odontogenic cysts Collagen diseases MMP-9 TIMP-2 |
title_short |
Comparative analysis of the immunohistochemical expression of collagen IV, MMP-9, and TIMP-2 in odontogenic cysts and tumors |
title_full |
Comparative analysis of the immunohistochemical expression of collagen IV, MMP-9, and TIMP-2 in odontogenic cysts and tumors |
title_fullStr |
Comparative analysis of the immunohistochemical expression of collagen IV, MMP-9, and TIMP-2 in odontogenic cysts and tumors |
title_full_unstemmed |
Comparative analysis of the immunohistochemical expression of collagen IV, MMP-9, and TIMP-2 in odontogenic cysts and tumors |
title_sort |
Comparative analysis of the immunohistochemical expression of collagen IV, MMP-9, and TIMP-2 in odontogenic cysts and tumors |
author |
Henriques, Águida Cristina Gomes |
author_facet |
Henriques, Águida Cristina Gomes Vasconcelos, Marcelo Gadella Galvão, Hebel Cavalcanti Souza, Lelia Batista de Freitas, Roseana de Almeida |
author_role |
author |
author2 |
Vasconcelos, Marcelo Gadella Galvão, Hebel Cavalcanti Souza, Lelia Batista de Freitas, Roseana de Almeida |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Henriques, Águida Cristina Gomes Vasconcelos, Marcelo Gadella Galvão, Hebel Cavalcanti Souza, Lelia Batista de Freitas, Roseana de Almeida |
dc.subject.por.fl_str_mv |
Immunohistochemistry Odontogenic cysts Collagen diseases MMP-9 TIMP-2 |
topic |
Immunohistochemistry Odontogenic cysts Collagen diseases MMP-9 TIMP-2 |
description |
Objective. The aim of this study was to evaluate the immunohistochemical expression of collagen IV, matrix metalloproteinase (MMP) 9 and tissue inhibitor of MMP (TIMP) 2 in dentigerous cysts (DCs), radicular cysts (RCs), keratocystic odontogenic tumors (KOTs), and ameloblastomas. Study design. Twenty cases of DCs, 20 RCs, 20 KOTs, and 20 ameloblastomas were selected and analyzed by immunohistochemistry. Results. Most DCs and RCs showed continuous and 50% staining for collagen IV in the basement membrane of the epithelium, whereas predominantly discontinuous thin and 50% staining was observed in KOTs and ameloblastomas, with a significant difference in staining percentage (P .001). MMP-9 was diffusely distributed and localized in both epithelial and mesenchymal cells of all of the lesions analyzed. The staining percentage was higher in the epithelium (P .058) and mesenchyme (P .005) of KOTs and ameloblastomas. Moreover, the distribution pattern, location, and percentage of expression of TIMP-2 were similar in the lesions studied, except for ameloblastoma, with a significant difference in staining percentage (P .001). Conclusion. These results demonstrate that the interaction between collagen IV, MMP-9, and TIMP-2 is an important factor for the establishment of differences in the biologic behavior of the odontogenic cysts and tumors studied. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2017-01-26T13:47:53Z |
dc.date.available.fl_str_mv |
2017-01-26T13:47:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
HENRIQUES, Águida Cristina Gomes et al. Comparative analysis of the immunohistochemical expression of collagen IV, MMP-9, and TIMP-2 in odontogenic cysts and tumors. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics, v. 112, n. 4, p. 468-475, 2011. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/21761 |
identifier_str_mv |
HENRIQUES, Águida Cristina Gomes et al. Comparative analysis of the immunohistochemical expression of collagen IV, MMP-9, and TIMP-2 in odontogenic cysts and tumors. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics, v. 112, n. 4, p. 468-475, 2011. |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/21761 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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